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v-Fos transformation effector binds with CD2 cytoplasmic tail

Chinese Science Bulletin 2006年第1期51卷 38-47页

作者： LI Ming ZHANG Weilun liu shilian liu Yanxin ZHENG Dexian National Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100005 China

We previously reported that v-Fos transformation effector (Fte-1) is a novel CD2 binding protein identified by yeast two hybrid system. In the present study, we further characterize the molecular properties and biological activity of the Fte-1. In vitro interaction analysis by an IAsys Resonant Mirror Bio- sensor further demonstrated that Fte-1 interacts with CD2 specifically, and the dissociation constant (Kd) of Fte-1-CD2 is 10?7 mol. The molecular section analysis showed that protein kinase C (PKC) phos- phorylation site at Ser238 of Fte-1, as confirmed by in vitro phosphorylation, is essential for the specific in- teraction with CD2. Jurkat T cells transfected with expression vector encoding for EGFP-Fte-1 fusion protein showed that Fte-1 displays a clustering dis- tribution in the cells. Upon stimulation by CD2 mono- clonal antibody T11, Fte-1 lost the character of clus- tering distribution and translocation to the plasma membrane, which were disrupted by mutation of Fte-1 (Ser238Gly), suggesting that Fte-1 could be co-localized with CD2 on the membrane, and Fte-1 phosphorylation at Ser238 is a crucial factor in CD2-mediated interaction with Fte-1. Suppression of Fte-1 expression by small interference RNA (siRNA) diminished the susceptibility of Jurkat T cells to apoptosis triggered by phorbol 12-myristate 13-ace- tate (PMA) and ionomycin, indicating that the bio- logical function of Fte-1 may be involved in the regu- lation of activation and apoptosis of T cells. These results provide further evidence that Fte-1 is a novel binding protein of CD2, and may function as a down- stream molecule in the CD2-mediated events.

关键词：磷酸化 Fte-1 CD2 结合蛋白 PKC

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Z-ajoene induces tumor cells to die by apoptosis

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Chinese Science Bulletin 1998年第13期43卷 1135-1140页

作者： Dexian Zheng Siqing Zhang Song Zhao Jimei Min Kui Wang shilian liu Chinese Acad Med Sci Inst Basic Med Sci State Key Lab Med Mol Biol New York NY 10005 USA Peking Union Med Coll New York NY 10005 USA Beijing Med Univ State Key Lab Nat & Biomimet Drugs Beijing 100083 Peoples R China Chinese Acad Med Sci Inst Hematol State Key Lab Expt Hematol Tianjin 300020 Peoples R China Peking Union Med Coll Tianjin 300020 Peoples R China

To unravel the pharmacological actions of garlic, a simple thiocompound, zajoene, was purified from the fresh cloves. Three tumor cell lines were subjected to treatment with zajoene at different concentrations in vitro and the morphological changes, DNA content of the cells and chromosome DNA fragmentation were observed by light microscopy, flow cytometry and agarose gel electrophoresis respectively. It was found that zajoene induced cell death by apoptosis in human HL60 promyelocytic leukemia cells, MGc803 gastric mucoid adenocarcinoma cells and Molt4 T lymphocyte leukemia cells. Western blot assay showed that z ajoene inhibited protooncogene bcl2 expression in the three different kinds of tumor cells, suggesting that zajoene may be a potential agent for tumor chemotherapy.

关键词： apoptosis of tumor cells z ajoene gene expression of bcl2.

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Cloning and identification of a novel binding protein of CD2 cytoplasmic domain

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Chinese Science Bulletin 2000年第24期45卷 2263-2267页

作者： SHAO Yuan XIAO Sheng ZHAI Wenjing LI Dong liu Yanxin liu shilian ZHENG Dexian State Key Laboratory of Experimental Hematology Institute of HematologyChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300020China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China

In order to understand why CD2 has a dual action of transduction of activation or apoptosis signals in T cells under different experimental conditions, we employed a yeast two-hybrid system to look for a binding protein of the cytoplasmic domain of CD2 which may be involved in this issue. A human T cell cDNA library was screened by a cDNA encoding the cytoplasmic domain of CD2 (Thr211-Gln336). The specificity of protein-protein interaction was verified by co-immunoprecipitation. The binding protein obtained, designated CD2cBP, was found to be homologous to v-fos transformation effector protein (Fte-1). As Fte-1 plays a role in cell transformation, growth, protein synthesis and protein-import into mitochondria, this result suggests that CD2cBP may be putatively involved in CD2-mediated signaling.

关键词： CD2 binding protein cytoplasmic domain yeast two-hybrid system signal transduction.

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PRMT5 suppresses DR4-mediated CCL20 release via NF-κB pathway

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Chinese Science Bulletin 2012年第9期57卷 4351-4355页

作者： WANG DongSheng liu Dan GAO Jing liu Min liu shilian liu YanXin ZHENG DeXian National Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100005China Department of Laboratory Medicine Affiliated Hospital of North Sichuan Medical CollegeNanchong 637000China

Construct expression vectors of pCMV-DR4-HA and pCMV-PRMT5-Flag,and transfect them into HEK293 cells to identify the interaction between TRAIL-R1 and PRMT5 and the molecular mechanism underlying DR4-mediated inhibition of chemokine CCL20 release via TRAIL receptor 1(DR4).Inflammatory cytokine was detected by RT-PCR and ELISA after TRAIL-R1 and/or PRMT5 transfection,***-κB activity was detected by Dual Luciferase Reporter Gene ***1/2 phosphorylation was analyzed by Western ***5 could inhibit DR4-activated NF-κB activity and ERK1/2 ***5 could inhibit NF-κB activition,ERK1/2 phosphorylation as well as CCL20 secretion via binding with DR4 in HEK293 cell,suggesting that PRMT5 may involve in DR4 dependent immune regulation.

关键词：介导 HEK293细胞 ELISA检测 RT-PCR 炎性细胞因子 blot分析免疫调节作用活性检测

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PI3K/Akt signaling pathway involved in regulation of T lymphocyte activation and apoptosis mediated by CD3ε

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Chinese Science Bulletin 2001年第7期46卷 568-570页

作者： ZHANG Jing, liu Yanxin, liu shilian & ZHENG DexianNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China National Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Beijing China

To study the expression and kinase activity of phosphatidylinositol 3’ -kinase (PI3K) and protein kinase B (PKB or Akt) during activation and apoptosis of human Jur-kat T lymphocytes (TJK) with stable expression of CD8ε chimera fused human CD8α extracellular and transmembrane domains to intracellular domain of mouse CD3ε, Western blot, kinase activities detection and immunoprecipitation were carried out It was shown that Jurkat cells with expression of wild type chimera CDSε died by apoptosis after continuous stimulation of anti-CD8 monoclonal antibody. The expressions of PI3K and Akt, and the kinase activity of Akt remarkably increased during the process. However, this phenomenon did not occur in the Jurkat cells (T1JK) with expression of the mutant of CD8ε chimera (Y170F), suggesting that PI3K/Akt signaling pathway is involved in activation and apoptosis of T lymphocyte mediated by CD3ε.

关键词： apoptosis CD8ε chimera T lymphocyte PI3K Akt.

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g-C_(3)N_(4)nanosheets coupled with CoSe_(2)as co-catalyst for efficient photooxidation of xylose to xylonic acid

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Green Energy & Environment 2025年第1期10卷 231-238页

作者： Qi Hao Yijun liu Ren Zou Ge Shi shilian Yang Linxin Zhong Wu Yang Xiao Chi Yunpeng liu Shimelis Admassie Xinwen Peng State Key Laboratory of Pulp and Paper Engineering South China University of TechnologyGuangzhou510641GuangdongChina Hainan Key Laboratory of Storage&Processing of Fruits and Vegetables Agricultural Products Processing Research InstituteChinese Academy of Tropical Agricultural SciencesZhanjiangGuangdong524001China Department of Chemistry and Centre for Advanced 2D Materials(CA2DM)National University of Singapore 3 Science Drive 3 Singapore117543Singapore School of Materials Science and Hydrogen Energy Foshan UniversityFoshan528231GuangdongChina Department of Chemistry Addis Ababa UniversityPO BOX 1176Addis AbabaEthiopia

Photocatalysis has emerged as an effective approach to sustainably convert biomass into value-added ***_(2)is a promising nonprecious,efficient cocatalyst for photooxidation,which can facilitate the separation of photogenerated electron–holes,increase the reaction rates,and enhance photocatalytic *** this work,we synthesized a stable and efficient photocatalysis system of CoSe_(2)/g-C_(3)N_(4)through attaching CoSe_(2)on g-C_(3)N_(4)sheets,with a yield of 50.12%for the selective photooxidation of xylose to xylonic *** light illumination,the photogenerated electrons were prone to migrating from g-C_(3)N_(4)to CoSe_(2)due to the higher work function of CoSe_(2),resulting in the accelerated separation of photogenerated electron–holes and the promoted ***,this study reveals the unique function of CoSe_(2),which can significantly promote oxygen adsorption,work as an electron sink and accelerate the generation of ·O_(2)^(-),thereby improving the selectivity toward xylonic acid over other *** work provides useful insights into the design of selective photocatalysts by engineering g-C_(3)N_(4)for biomass high-value utilization.

关键词： Biomass photooxidation Hemicelluloses Xylose g-C_(3)N_(4) Xylonic acid

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A novel Anti-DR5 Chimeric Antibody and Epirubicin Suppress Tumor Growth Synergizely

A novel Anti-DR5 Chimeric Antibody and Epirubicin Suppress T...

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第八届全国免疫学学术大会

作者： Yuhe Qiu Zhixin Zhang Juan Shi shilian liu Yanxin liu Dexian Zheng National Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences & Peking Union Medical College Beijing Red Cross Blood Center

Tumor necrosis factor(TNF)-related apoptosis-inducing ligand(TRAIL)induces apoptosis in a variety of tumor *** receptor 2(DR5)is detected at high-level expression in tumor and transformed cells and clinical tumor specimens,but at low-level expression in most normal cells and tissues,thus regards as an attractive target for cancer *** the present study,a novel mouse-human chimeric antibody based on AD5-10 was established,named as HMCAZ5,and stably expressed in *** purified HMCAZ5 possesses a high affinity equal to the parental mouse antibody,induces apoptosis in various cancer

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RIP1 Expression and Ubiquitination Modulates TRAILinduced Apoptosis and Autophagy in Monocytes/Macrophages

RIP1 Expression and Ubiquitination Modulates TRAILinduced Ap...

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第八届全国免疫学学术大会

作者： Zhengyu Yao Peng Zhang Yanxin liu Juan Shi shilian liu Dexian Zheng National Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences & Peking Union Medical College

Monocyte/macrophage,responsible for defending against diverse pathogens,plays a crucial role in the innate immune *** lifespan of monocyte/macrophage is determined by the homeostatic balance between survival and *** the present study,we report that tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)-triggered both apoptosis and autophagy in the monocyte/*** protected

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TRAIL suppresses CXCR4-mediated human breast cancer metastasis by upregulation of miR-146a expression

TRAIL suppresses CXCR4-mediated human breast cancer metastas...

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第八届全国免疫学学术大会

作者： Dongsheng Wang Dan liu Jing Gao Min liu shilian liu Minghong Jiang Yanxin liu Dexian Zheng National Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences & Peking Union Medical College Department of Laboratory Medicine the Affiliated Hospital of North Sichuan Medical College

Tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)is considered as a promising agent for cancer therapy,since it induces apoptosis specifically in various cancer cells without toxic side effects in most of normal *** from apoptosis,TRAIL can also induce non-apoptotic signals,such as proliferation and metastasis of cancer *** the present study,we observed that TRAIL suppressed CXCR4-mediated human breast cancer metastasis through upregulating miR-146a expression controlled by NF-κ*** receptor

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Two tyrosines in CD3ε-ITAM are required to induce T lymphocyte apoptosis

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Chinese Science Bulletin 1998年第17期43卷 1480-1485页

作者： ZHENG Dexian 1,2, HE Yiping+1, liu Yanxin+1, ZHENG Yong,XIAO Sheng+1, liu Yin+1 and liu shilian+1 1. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Beijing China State Key Laboratory of Experimental Hematology Institute of Hematology Chinese Academy of Medical Sciences and Peking Union Medical College Tinjin China

CD3ε of T cell antigen receptor complex (TCR/CD3) plays an important role in the resembling of the complex and activation signaling through its conservative immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic tail. Previous study showed that a chimera molecule, consisting of the extracellular-transmembrane domain of human CD8α fused to the cytoplasmic domain of CD3ε, induced apoptosis of T lymphocytes, indicating that apoptotic signals were transduced through the CD3ε- ITAM. To elineate involvement of the two tyrosines in apoptotic signaling pathway, cDNAs with mutations at Y170F, Y181F and Y170F/Y181F in CD8-ε-ITAM were made by point mutation and PCR, and then cloned into pcDNA3 eukaryotic expression vectors. Stable expression cell lines were established after transfection of the expression vectors into CD8+- Jurkat T lymphocytes. Stimulation of these cell lines with anti-CD8 monoclonal antibody showed that only the cells with expression of wild type chimera CD8-ε died by apoptosis, but not those cells with expressions of mutated CD8-ε chimera, indicating that the two tyrosines in CD3ε-ITAM were required for the apoptotic signal transduction in T lymphocytes.

关键词： apoptosis CD3ε-ITAM tyrosine.

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