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Journal of Integrative Agriculture 2017年第10期16卷 2264-2273页

作者： liu ren-qiang GE Jin-ying WANG Jin-ling SHAO Yu ZHANG Hui-lei WANG Jin-liang WEN Zhi-yuan BU Zhi-gao State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150069 P.R.China College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot 010018 P.R. China

Middle East respiratory syndrome coronavirus （MERS-CoV）, a member of the Coronavifidae family, is the causative pathogen for MERS that is characterized by high fever, pneumonia, acute respiratory distress syndrome （ARDS）, as well as extrapul- monary manifestations. Currently, there are no approved treatment regimens or vaccines for MERS. Here~ we generated recombinant nonvirulent Newcastle disease virus （NDV） LaSota strain expressing MERS-CoV S protein （designated as rLa- MERS-S）, and evaluated its immunogenicity in mice and Bactrian camels. The results revealed that rLa-MERS-S showed similar growth properties to those of LaSota in embryonated chicken eggs, while animal immunization studies showed that rLa-MERS-S induced MERS-CoV neutralizing antibodies in mice and camels. Our findings suggest that recombinant rLa- MERS-S may be a potential MERS-CoV veterinary vaccine candidate for camels and other animals affected by MERS.

关键词： Newcastle disease virus MERS-CoV neutralizing antibodies camels

Generation of recombinant rabies virus ERA strain applied to virus tracking in cell infection

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Journal of Integrative Agriculture 2019年第10期18卷 2361-2368页

作者： ZHAO Dan-dan SHUAI Lei GE Jin-ying WANG Jin-liang WEN Zhi-yuan liu ren-qiang WANG Chong WANG Xi-jun BU Zhi-gao State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009P.R.China

The mechanism of rabies virus (RABV) infection still needs to be further characterized. RABV particle with self-fluorescent is a powerful viral model to visualize the viral infection process in cells. Herein, based on a reverse genetic system of the Evelyn-Rokitnicki-Abelseth (rERA) strain, we generated a recombinant RABV rERA-N/mCherry strain that stably expresses an additional ERA nucleoprotein that fuses with the red fluorescent protein mCherry (N/mCherry). The rERA-N/mCherry strain retained growth property similar to the parent strain rERA in vitro. The N/mCherry expression showed genetic stability during passage into mouse neuroblastoma (NA) cells and did not change the virulence of the vector. The rERA-N/mCherry strain was then utilized as a visual viral model to study the RABV-cell binding and internalization. We directly observed the red self-fluorescence of rERA-N/mCherry particles binding to the cell surface, and further co-localizing with clathrin in the early stage of infection in NA cells by fluorescence microscopy. Our results showed that the rERA-N/mCherry strain uses clathrin-dependent endocytosis to enter cells, which is consistent with the well-known mechanism of RABV invasion. The recombinant RABV rERA-N/mCherry thus appears to have the potential to be an effective viral model to further explore the fundamental molecular mechanism of rabies neuropathogenesis.

关键词： rabies virus self-fluorescence binding internalization

Commentary on -＂Comparison of the efficacy and safety of once-daily dosing and on-demand use of udenafil for type 2 diabetic patients with erectile dysfunction＂

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Asian Journal of Andrology 2015年第5期17卷 854-854页

作者： De-Hong Cao Liang-ren liu qiang Wei Department of Urology West China Hospital Sichuan University ChengduSichuan Province China

Dear Editor, We read with interest of the article written by Park et al. They performed a randomized controlled trial to compare efficacy and safety between 50 mg once-daily and 200 mg on-demand dosing use of udenafil for the treatment of type 2 diabetic patients with erectile dysfunction （ED）. Their findings indicated that the most common drug-related adverse events （AEs） were flushing （8,9% vs 2,5%） and headache （3.8% vs 1.3%） between the on-demand group and the daily-dosed group. Although the authors believed that the udenafil was well-tolerated in both groups, unfortunately, they failed report the total incidence of AEs to assess the safety of the two groups.

关键词： daily-dosed compare well-tolerated

Optimizing the key parameter to accelerate the recovery of AMOC under a rapid increase of greenhouse gas forcing

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 39-45页

作者： Haolan ren Fei Zheng Tingwei Cao qiang Wang International Center for Climate and Environment Science(ICCES) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina College of Oceanography Hohai UniversityNanjingChina CAS Key Laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of Sciences and Pilot National Laboratory for Marine Science and Technology(Qingdao)QingdaoChina

Atlantic Meridional Overturning Circulation(AMOC)plays a central role in long-term climate variations through its heat and freshwater transports,which can collapse under a rapid increase of greenhouse gas forcing in climate *** studies have suggested that the deviation of model parameters is one of the major factors in inducing inaccurate AMOC *** this work,with a low-resolution earth system model,the authors try to explore whether a reasonable adjustment of the key model parameter can help to re-establish the AMOC after its *** a new optimization strategy,the extra freshwater flux(FWF)parameter is determined to be the dominant one affecting the AMOC’s *** traditional ensemble optimal interpolation(EnOI)data assimilation and new machine learning methods are adopted to optimize the FWF parameter in an abrupt 4×CO_(2) forcing experiment to improve the adaptability of model parameters and accelerate the recovery of *** results show that,under an abrupt 4×CO_(2) forcing in millennial simulations,the AMOC will first collapse and then re-establish by the default FWF parameter ***,during the parameter adjustment process,the saltier and colder sea water over the North Atlantic region are the dominant factors in usefully improving the adaptability of the FWF parameter and accelerating the recovery of AMOC,according to their physical relationship with FWF on the interdecadal timescale.

关键词： Recovery of AMOC 4×CO_(2)forcing Key parameter Parameter estimation Data assimilation Machine learning

一次切断低压诱发的暖区深对流与异常副热带锋及其平流层-对流层交换

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地球物理学报 2014年第1期57卷 21-30页

作者：施春华李慧郑彬郭栋刘仁强南京信息工程大学大气科学学院气象灾害省部共建重点实验室南京210044 南京信息工程大学语言文化学院南京210044 中国气象局广州热带海洋气象研究所热带季风重点开放实验室广州510080

采用Cloudsat/CPR云雷达,FY2C/TBB亮温,Aura/MLS大气成分等卫星遥感资料,结合ECMWF气象分析资料和HYSPLIT4轨迹模式,研究了2009年6月一次东亚切断低压的暖区深对流和异常副热带锋面的结构和演变.分析表明,由于低压切断前的旧槽背景,在... 详细信息

采用Cloudsat/CPR云雷达,FY2C/TBB亮温,Aura/MLS大气成分等卫星遥感资料,结合ECMWF气象分析资料和HYSPLIT4轨迹模式,研究了2009年6月一次东亚切断低压的暖区深对流和异常副热带锋面的结构和演变.分析表明,由于低压切断前的旧槽背景,在低涡的近成熟期,内部冷、暖锋降水偏弱,边沿的高空副热带锋面异常发展到对流层底部,低空西南暖湿水汽在副热带锋前聚集,形成千公里长的暖区深对流降水带.随着该锋面的快速东移,副热带锋区进入原暖区雨带,锋区热力间接次级环流的强上升支,加强了锋下冷侧(原暖湿区)的深对流,但该锋面阻挡了来自暖侧的水汽补充,降水结束.该异常副热带锋区还发生了强烈的平流层-对流层相互交换,在高空急流出口区的下方,平流层1.5PVU等位涡线向下入侵可达5.5km(约500hPa)处,锋下向上的深对流注入可达10km,在入侵-注入混合区,臭氧和水汽的散点图上出现了二者浓度双高和双低的特殊气团.

关键词：切断低压深对流副热带锋平流层-对流层交换暖区

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光纤传感技术测量曲率的研究进展

传感器与微系统 2011年第6期30卷 5-7页

作者：狄海廷刘仁强东北林业大学工程技术学院黑龙江哈尔滨150040 上海交通大学机械与动力工程学院上海200240

光纤传感器具有抗电磁干扰、体积小、耐腐蚀及可集成等诸多优点,已被国内外公认为最具有发展前途的高新技术产业之一。在材料结构领域中,负载结构下的变形曲率是一个非常重要的结构参数。在分析传统曲率测量技术的局限性和光纤传感技术... 详细信息

光纤传感器具有抗电磁干扰、体积小、耐腐蚀及可集成等诸多优点,已被国内外公认为最具有发展前途的高新技术产业之一。在材料结构领域中,负载结构下的变形曲率是一个非常重要的结构参数。在分析传统曲率测量技术的局限性和光纤传感技术特点的基础上,重点介绍了不同类型光纤传感器测量曲率的研究现状,并分析了它们各自的特性。最后,提出高集成化和智能化是光纤传感器的发展趋势。

关键词：光纤传感器曲率测量变形

基于小波变换和LSTM神经网络的格陵兰岛近海海域海平面变化预测

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海洋科学 2023年第8期47卷 7-16页

作者：赵健刘仁强中国石油大学(华东)海洋与空间信息学院山东青岛266580

海平面变化包含多种不同时间尺度信息,传统的预测方法仅对海平面变化趋势项、周期项进行拟合,难以利用海平面变化的不同时间尺度信号,使得预测精度不高。本文基于深度学习的预测模型,提出一种融合小波变换(wavelet transform,WT)与LSTM(... 详细信息

海平面变化包含多种不同时间尺度信息,传统的预测方法仅对海平面变化趋势项、周期项进行拟合,难以利用海平面变化的不同时间尺度信号,使得预测精度不高。本文基于深度学习的预测模型,提出一种融合小波变换(wavelet transform,WT)与LSTM(long short-term memory,LSTM)神经网络的海平面异常组合预测模型。首先利用小波分解得到反映海平面变化总体趋势的低频分量和刻画主要细节信息的高频分量;然后通过LSTM神经网络对代表不同时间尺度的各个分量预测和重构,实现海平面变化的非线性预测。基于该模型的海平面变化预测的均方根误差、平均绝对误差和相关系数分别为12.76 mm、9.94 mm和0.937,预测精度均优于LSTM和EEMD-LSTM预测模型,WT-LSTM组合模型对区域海平面变化预测具有较好的应用价值。

关键词：海平面异常小波变换长短时记忆网络海平面变化预测

The emerging role of nitric oxide in the synaptic dysfunction of vascular dementia

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Neural Regeneration Research 2025年第2期20卷 402-415页

作者： Xiaorong Zhang Zhiying Chen Yinyi Xiong Qin Zhou Ling-qiang Zhu Dan liu Department of Pathology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Jiujiang Clinical Precision Medicine Research Center JiujiangJiangxi ProvinceChina Center for Cognitive Science and Transdisciplinary Studies Jiujiang UniversityJiangxi ProvinceChina Department of Neurology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Rehabilitation Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

With an increase in global aging,the number of people affected by cerebrovascular diseases is also increasing,and the incidence of vascular dementia-closely related to cerebrovascular risk-is increasing at an epidemic ***,few therapeutic options exist that can markedly improve the cognitive impairment and prognosis of vascular dementia *** in Alzheimer’s disease and other neurological disorders,synaptic dysfunction is recognized as the main reason for cognitive *** oxide is one of the ubiquitous gaseous cellular messengers involved in multiple physiological and pathological processes of the central nervous ***,nitric oxide has been implicated in regulating synaptic plasticity and plays an important role in the pathogenesis of vascular *** review introduces in detail the emerging role of nitric oxide in physiological and pathological states of vascular dementia and summarizes the diverse effects of nitric oxide on different aspects of synaptic dysfunction,neuroinflammation,oxidative stress,and blood-brain barrier dysfunction that underlie the progress of vascular ***,we propose that targeting the nitric oxide-sGC-cGMP pathway using certain specific approaches may provide a novel therapeutic strategy for vascular dementia.

关键词： endoplasmic reticulum stress endothelial nitric oxide synthase gene therapy nitric oxide NO-sGC-cGMP pathway synaptic dysfunction vascular dementia

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基于激光扫描的大锻件三维尺寸测量

机械设计与研究 2011年第3期27卷 82-85页

作者：代杰赵现朝刘仁强上海交通大学机械系统与振动国家重点实验室上海200240

锻压过程中对大锻件的准确快速测量能提高大锻件的制造能力和减少资源浪费。本文介绍了基于双轴直角坐标运动执行机构和激光扫描测距传感器的测量系统,实现热态锻件尺寸的快速准确测量,采用椭圆的最小二乘法拟合锻件截面并获取锻件关键... 详细信息

锻压过程中对大锻件的准确快速测量能提高大锻件的制造能力和减少资源浪费。本文介绍了基于双轴直角坐标运动执行机构和激光扫描测距传感器的测量系统,实现热态锻件尺寸的快速准确测量,采用椭圆的最小二乘法拟合锻件截面并获取锻件关键尺寸,并计算锻件轴线直线度并进行误差分析。通过实验验证该测量方案的可行性和重复性。

关键词：锻件测量激光扫描最小二乘法椭圆拟合直线度误差

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运用“四参数法”判别低效无效注水循环体

大庆石油地质与开发 2009年第3期28卷 74-78页

作者：付艳波刘仁强大庆油田有限责任公司第七采油厂黑龙江大庆163517 大庆油田有限责任公司勘探开发研究院黑龙江大庆163712

正确识别低效无效注水循环体，才能有针对性地采取油水井调整措施。通过开展低效无效循环体特征研究，建立了“四参数判别体系”方法判断低效无效注采循环体的可疑井组，结合高精度注入、产出剖面测试资料解释结果进行分析，判断低效无... 详细信息

正确识别低效无效注水循环体，才能有针对性地采取油水井调整措施。通过开展低效无效循环体特征研究，建立了“四参数判别体系”方法判断低效无效注采循环体的可疑井组，结合高精度注入、产出剖面测试资料解释结果进行分析，判断低效无效循环层。同时利用静态资料，对可疑的低效无效循环层位加以确认，利用油水井动态资料判断循环体形成的时间，为采取措施减缓低效无效循环、提高注入水利用率和驱油效率提供一定的依据。通过实例分析表明，建立在动静态资料基础上的“四参数判别体系”方法能够在一个开发区大量的油水井中圈定低效无效注水循环的可疑井组，为进一步寻找具体层位指明了方向，是识别油田注水开发中低效无效注水循环体的一种有效实用的方法。

关键词：低效无效循环高渗透条带注入水利用率驱油效率