检索结果-南通市图书馆

Agricultural Biotechnology 2012年第1期1卷 34-36,50页

作者： Zhiqiang mei Chunhong LI Chenggang DUAN Xiaoyan liu Research Center for Preclinical Medicine Luzhou Medical College Research Center for Drug and Functional Food Luzhou Medical College

[ Objective] This study aimed to explore a modified method for improving the efficiency of RAPD technology. [ Method] Longans collected from Si- chuan, Fujian, Guangxi, Guangdong and Hainan were used as experimental materials. Total genomic DNA was extracted from langan leaves with modified CTAB method. 18 random primers were selected for RAPD amplification with ramp rates of 0.3 and 3.0℃/s, respectively. Effects of different ramp rates on RAPD am- plification were compared and the relationship among longan samples was analyzed. [ Result] The resolution and yield could be improved significantly by declining the ramp rate from 3.0 to 0.3℃/s. Cluster analysis showed that similarity coefficient of the five varieties of longan ranged from 0.69 to 0.76. [ Conclusion] This study had confirmed that reducing ramp rate could significantly increase the resolution and yield of RAPD technology, which had provided a new method to improve the efficiency of RAPD.

关键词： RAPD Ramp Longan Efficiency

Development and utilization of a new chemically-induced soybean library with a high mutation density

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2017年第1期59卷 60-74页

作者： Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong liu Jinhui Lei Ying liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming liu Nang Myint Phyu Sin Htwe Lin liu Bingfu Guo Jian Song Bing Tan Guifeng liu Maiquan Li Xianli Zhang Bo liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan qiu National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural Science Soybean Research Institute Heilongjiang Academy of Agricultural Sciences College of Agriculture Yangtze University

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate（EMS）-induced soybean（Glycine max） population,consisting of 21,600 independent M_2 lines, was *** 1,000 M_（4（5））families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll（Chl） content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene（ChlI1a） results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

关键词： soybean mutant yellow chemically golden phenotype genomics mutation Glycine chloroplast

Long-term outcomes of high-risk percutaneous coronary interventions under extracorporeal membrane oxygenation support:An observational study

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第16期10卷 5266-5274页

作者： Yi-Xiong Huang Zheng-Ming Xu Li Zhao Yi Cao Yu Chen Yi-Gang qiu Ying-Ming liu Peng-Yu Zhang Jiang-Chun He Tian-Chang Li Medical School of Chinese People’s Liberation Army Beijing 100853China Department of Cardiology Sixth Medical Center of Chinese People’s Liberation Army General HospitalBeijing 100048China

BACKGROUND Venoarterial extracorporeal membrane oxygenation(VA-ECMO)offers hemodynamic support for patients undergoing high-risk percutaneous coronary interventions(PCIs).However,long-term outcomes associated with VA-ECMO have not previously been *** To explore long-term outcomes in high-risk cases undergoing PCI supported by *** In the present observational cohort study,61 patients who received VA-ECMOsupported high-risk PCI between April 2012 and January 2020 at the Sixth Medical Center of Chinese People’s Liberation Army General Hospital were *** endpoint characteristics such as all-cause mortality,repeated cardiovascular diseases,and cardiac death were *** Among 61 patients,three failed stent implantation due to chronic total occlusions with severely calcified *** patient showed VA-ECMO intolerance because of high left ventricular *** was successfully performed in 57 patients(93.4%).The in-hospital mortality was 23.0%,and the overall survival was 45.9%,with a median follow-up period of 38.6(8.6-62.1)*** VA-ECMO can be used as a support in patients undergoing high-risk PCI as it is associated with favorable long-term patient survival.

关键词： High-risk percutaneous coronary intervention Venoarterial extracorporeal membrane oxygenation Overall survival Long-term survival

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于灰度分布特征的层次掌纹识别方法

电子与信息学报 2007年第8期29卷 1821-1825页

作者：吴介裘正定孙冬梅北京交通大学信息所北京100044

目前掌纹识别算法主要集中在对掌纹图像所切取的ROI区域的研究,而对原始手部图像的灰度分布特征则讨论较少。在一定光照条件下,掌部不同位置的主线、皱纹和表层皮肤颜色的深浅在灰度图像上各自对应了不同的灰度级。该文提出一种利用手... 详细信息

目前掌纹识别算法主要集中在对掌纹图像所切取的ROI区域的研究,而对原始手部图像的灰度分布特征则讨论较少。在一定光照条件下,掌部不同位置的主线、皱纹和表层皮肤颜色的深浅在灰度图像上各自对应了不同的灰度级。该文提出一种利用手部尺寸和角度信息完成粗分类,借助单元信息熵的概念来分析手部图像的灰度分布特征从而完成细分类的层次掌纹识别方法。该方法不同于传统的对ROI区域进行特征提取的方法,直接利用整幅人手图像完成分类识别。在99类共990幅手部图像的数据库上进行的实验结果与PCA和LDA算法的对比表明,该算法具有比传统算法更高的鲁棒性,识别率也得到了较大幅度的提高。

关键词：掌纹识别手部尺度灰度分布单元信息熵层次

应用基于GPU的SPH方法模拟二维楔形体入水砰击问题

在线全文

学校读者我要写书评

暂无评论

计算力学学报 2013年第S1期30卷 174-177页

作者：王珏邱流潮中国农业大学应用力学系北京100083

光滑粒子流体动力学(SPH)法是一种无网格的拉格朗日数值方法,广泛应用于计算流体领域模拟复杂自由表面流问题.SPH方法的主要缺点就是计算量过大,而基于GPU的并行计算方法可使SPH计算得到有效加速.本文应用基于GPU的SPH并行计算方法研究... 详细信息

光滑粒子流体动力学(SPH)法是一种无网格的拉格朗日数值方法,广泛应用于计算流体领域模拟复杂自由表面流问题.SPH方法的主要缺点就是计算量过大,而基于GPU的并行计算方法可使SPH计算得到有效加速.本文应用基于GPU的SPH并行计算方法研究了二维楔形体的入水砰击问题。数值计算结果与文献中对应的解析解比较一致,验证了基于GPU的SPH方法的精度和可靠性.仿真结果同时显示基于GPU的并行计算方法可使SPH计算速度得到显著提高.

关键词：光滑粒子流体动力学 GPU 楔形体入水砰击问题数值模拟

在线全文

学校读者我要写书评

暂无评论

技术创新扩散的人力资本因素分析

社会科学家 2011年第5期26卷 59-62,67页

作者：赵如邱成梅四川大学经济学院四川成都610068

技术创新扩散是一国、地区或企业获得新技术的重要渠道,技术创新的重要意义也在于实现扩散,从而改变人们的生产生活方式。技术创新扩散总是在由多种因素构成的环境中进行,其中人力资本在推动技术创新扩散中扮演着重要角色,包括企业家的... 详细信息

技术创新扩散是一国、地区或企业获得新技术的重要渠道,技术创新的重要意义也在于实现扩散,从而改变人们的生产生活方式。技术创新扩散总是在由多种因素构成的环境中进行,其中人力资本在推动技术创新扩散中扮演着重要角色,包括企业家的决策作用,技术人员提供的技术支持以及销售人员与市场的沟通等方面。

关键词：技术创新扩散企业家技术人员销售人员

Study on the Variation in Main Chemical Composition of Blumea balsamifera in Different Growth Stages

在线全文

学校读者我要写书评

暂无评论

Medicinal Plant 2017年第4期8卷 6-9页

作者： mei HUANG Ce CHEN Liwei liu Zhenxia CHEN Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China Hainan Provincial Engineering Research Center for Blumea Balsamifera

[Objectives]To study the variation in main chemical composition of Blumea balsamifera in different growth stages,and to provide a theoretical basis to determine appropriate harvest period.[Methods]The l-borneol of the plant was determined by gas chromatography( GC)with methyl salicylate as internal standard,and total flavonoids were determined by the Aluminum nitrate colorimetry method of Ultraviolet-Visible spectrophotometry. The analysis of variance was used for statistical analysis of the l-borneol and total flavonoids of B. balsamifera in different months and different plant age.[Results]There were differences in chemical ingredients of B. balsamifera in different months of the *** content of l-borneol in October was the highest,but there was no significant difference from August to December in l-borneol content( P >0. 05). l-borneol content of B. balsamifera in three ages was the highest compared with other ages,and there was a significant difference with B. balsamifera in one year or less( P < 0. 05). Relatively speaking,total flavonoids in June,August and November were higher; total flavonoids content of B. balsamifera in two ages was the highest compared with other ages,and there was a significant difference with other ages( P <0. 05).[Conclusions]If l-borneol is taken as an indicator,for extracting Aipan( l-borneol),it can be harvested from September to midDecember,but October is the best. And it can be harvested at least three years. If the content of total flavonoids is taken as an indicator,it should be harvested in November. And it can be harvested 2-3 years.

关键词： Blumea balsamifera l-borneol Total flavones Different growth ages

Characterization of microstructural length scales in directionally solidified Sn-36%Ni peritectic alloy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transactions of Nonferrous Metals Society of China 2013年第8期23卷 2446-2453页

作者： Peng PENG Xin-zhong LI Yan-qing SU Dong-mei liu Jing-jie GUO Heng-zhi FU Harbin Inst Technol Sch Mat Sci & Engn Harbin 150001 Peoples R China

Sn-36%Ni peritectie alloys were directionally solidified at different growth rates under a constant temperature gradient （20 K/mm）, the dependences of microstructural characteristic length scales on the growth rate were investigated. Experimental results are presented, including primary and higher order dendrite arm spacings 21, 22, 23 and dendrite tip radius R of primary NisSn2 phase. Comparisons between the theoretical predictions and the experimental results show that, for the primary dendrites, 21=335.882v-0.21, which is in agreement with the Kurz-Fisher model; for the secondary dendrites, λ2=44.957v-0.277, which is consistent with the Bouchard-Kirkaldy model; for the tertiary dendrites, λ3=40.512v-0.274; for the dendrite tip radius, R=22.7v-0.36. The experimental results also show that the 21/22 changes greatly with increasing growth rate while the 21/23 has no significant change, indicating that tertiary dendrite arms have a more similar growth characteristics to primary dendrites compared with secondary dendrites. The λ1/R ranges from 2 to 2.3 with the increase of growth rate. Key words： Sn-Ni alloy; directional solidification; dendrite arm spacing; dendrite tip radius

关键词： Sn-Ni alloy directional solidification dendrite arm spacing dendrite tip radius

Relation Entropy and Transferable Entropy Think of Aggregation on Group Decision Making

在线全文

学校读者我要写书评

暂无评论

Systems Science and Systems Engineering 2002年第1期11卷 13-18页

作者： CHENG Qi-yue, qiu Wan-hua, liu Xiao-feng School of Management, Beijing University of Aeronautics and Astronautics, Beijing 100083, China School of Management Beijing University of Aeronautics and Astronautics Beijing 100083 China

In this paper, aggregation question based on group decision making and a single decision making is studied. The theory of entropy is applied to the sets pair analysis. The system of relation entropy and the transferable entropy notion are put. The character is studied. An potential by the relation entropy and transferable entropy are defined. It is the consistency measure on the group between a single decision making. We gained a new aggregation effective definition on the group misjudge.

关键词： group decision making relation entropy transferable entropy