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Journal of Integrative Agriculture 2017年第1期16卷 36-46页

作者： JIA Li-cong ZHAI Hong HE Shao-zhen YANG Yu-feng liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/China Agricultural University Beijing 100193 P.R. China

The somatic hybrid KT1 was previously obtained from protoplast fusion between sweetpotato （Ipomoea batatas （L.） Lam.） cv. Kokei No. 14 and its wild relative I. triloba L. However, its genetic and epigenetic variations have not been investigated. This study showed that KT1 exhibited significantly higher drought tolerance compared to the cultivated parent Kokei No. 14. The content of proline and activities of superoxide dismutase （SOD） and photosynthesis were significantly increased, while malonaldehyde （MDA） content was significantly decreased compared to Kokei No. 14 under drought stress. KT1 also showed higher expression level of well-known drought stress-responsive genes compared to Kokei No. 14 under drought stress. Amplified fragment length polymorphism （AFLP） and methylation-sensitive amplified polymorphism （MSAP） analyses indicated that KT1 had AFLP and MSAP band patterns consisting of both parent specific bands and changed bands. Fur- ther analysis demonstrated that in KT1. the proportions of Kokei No. 14 specific genome components and methylation sites were much greater than those of I. triloba. KT1 had the same chloroplast and mitochondrial genomes as Kokei No. 14. These results will aid in developing the useful genes ofI. triloba and understanding the evolution and phylogeny of the cultivated sweetpotato.

关键词： drought tolerance epigenetic variation genetic variation Ipomoea batatas (L.) Lain. I. triloba L. somatic hybrid

A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato

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Journal of Integrative Agriculture 2016年第9期15卷 1968-1982页

作者： WANG Yan-nan LI Yan ZHANG Huan ZHAI Hong liu qing-chang HE Shao-zhen College of Agronomy & Biotechnology China Agricultural University

A plastidic adenosine triphosphate（ATP）/adenosine diphosphate（ADP） transporter（AATP） is responsible for importing ATP from the cytosol into plastids. In dicotyledonous plants, increasing ATP supply is a potential way to facilitate anabolic synthesis in heterotrophic plastids. In this study, a gene encoding the AATP protein, named Ib AATP, was isolated from sweetpotato（Ipomoea batatas（L.） Lam.）. Transcripts of Ib AATP were predominantly detected in the storage roots and leaves and were induced by exogenous sucrose and subjected to circadian rhythm. Transient expression of Ib AATP in tobacco and onion epidermal cells revealed the plastidic localization of Ib AATP. The overexpression of Ib AATP in sweetpotato significantly increased the starch and amylose contents and led to enlarged starch granules. The IbA ATP-overexpressing plants showed altered fine structure of amylopectin, which contained an increased proportion of chains with a degree of polymerization（DP） of 10–23 and a reduced number of chains with a DP of 5–9 and 24–40. In addition, starch from the transgenic plants exhibited different pasting properties. The transcript levels of starch biosynthetic genes, including Ib AGP, Ib GBSSI, Ib SSIIV, and Ib SBE, were differentially regulated in the transgenic plants. These results revealed the explicit role of Ib AATP in the starch biosynthesis of sweetpotato and indicated that this gene has the potential to be used to improve starch content and quality in sweetpotato and other plants.

关键词： sweetpotato Ib AATP starch content and composition starch granule size starch structure pasting properties

SSR fingerprinting of 203 sweetpotato (Ipomoea batatas (L.) Lam.) varieties

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Journal of Integrative Agriculture 2018年第1期17卷 86-93页

作者： MENG Yu-sha ZHAO Ning LI Hui ZHAI Hong HE Shao-zhen liu qing-chang Key Laboratory of Sweetpotate Biology and Biotechnolog Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization of Ministry of Education China Agricultural University Beijing 100193 P.R. China

Simple sequence repeat （SSR） markers have been shown to be a powerful tool for varieties identification in plants. How- ever, SSR fingerprinting of sweetpotato varieties has been a little reported. In this study, a total of 1 294 SSIR primer pairs, including 1 215 genomic-SSR and 79 expressed sequence tag （EST）-SSR primer pairs, were screened with sweetpotato varieties Zhengshu 20 and Luoxushu 8 and their 2 F1 individuals randomly sampled, and 273 and 38 of them generated polymorphic bands, respectively. Four genomic-SSR and 3 EST-SSR primer pairs, which showed good polymorphism, were selected to amplify 203 sweetpotato varieties and gave a total of 172 bands, 85 （49.42%） of which were polymorphic. All of the 203 sweetpotato varieties showed unique fingerprint patterns, indicating the utility of SSR markers in variety iden- tification of this crop. Polymorphism information content （PIC） ranged from 0.5824 to 0.9322 with an average of 0.8176. SSR-based genetic distances varied from 0.0118 to 0.6353 with an average of 0.3100 among these varieties. Thus, these sweetpotato varieties exhibited high levels of genetic similarity and had distinct fingerprint profiles. The SSR fingerprints of the 203 sweetpotato varieties have been successfully constructed. The highly polymorphic SSR primer pairs developed in this study have the potential to be used as core primer pairs for variety identification, genetic diversity assessment and linkage map construction in sweetpotato and other plants.

关键词： EST-SSR fingerprinting genetic distance genomic-SSR sweetpotato

Transcriptome profiling reveals insights into the molecular mechanism of drought tolerance in sweetpotato

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Journal of Integrative Agriculture 2019年第1期18卷 9-23页

作者： ZHU Hong ZHOU Yuan-yuan ZHAI Hong HE Shao-zhen ZHAO Ning liu qing-chang Key Laboratory of Sweetpotato Biology and Biotechnology Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of Education/College of Agronomy & BiotechnologyChina Agricultural University

Sweetpotato,Ipomoea batatas(L.) Lam.,is a globally important food crop and usually grown on arid-and semi-arid ***,investigating the molecular mechanism of drought tolerance will provide important information for the improvement of drought tolerance in this *** this study,transcriptome analysis of the drought-tolerant sweetpotato line Xushu 55-2 was conducted on Illumina HiSeq 2500 platform.A total of 86.69 Gb clean data were generated and assembled into 2 671 693 contigs,222 073 transcripts,and 73 636 *** total,11 359 differentially expressed genes(DEGs) were identified after PEG6000 treatment,in which 7 666 were up-regulated and 3 693 were *** the 11 359 DEGs,10 192 DEGs were annotated in at least one database,and the remaining 1 167 DEGs were *** acid(ABA),ethylene(ETH),and jasmonic acid(JA) signalling pathways play a major role in drought tolerance of ***-inducible transcription factors were identified,some of which have been reported to be associated with drought tolerance and others are unknown in *** addition,7 643 SSRs were *** study not only reveals insights into the molecular mechanism of drought tolerance in sweetpotato but also provides the candidate genes involved in drought tolerance of this crop.

关键词： sweetpotato Ipomoea batatas (L.) Lam. transcriptome drought tolerance molecular mechanism SSR markers

Analysis of genetic diversity and population structure in sweetpotato using SSR markers

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Journal of Integrative Agriculture 2023年第11期22卷 3408-3415页

作者： liu Cheng ZHAO Ning JIANG Zhi-cheng ZHANG Huan ZHAI Hong HE Shao-zhen GAO Shao-pei liu qing-chang Key Laboratory of Sweetpotato Biology and Biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of Education/College of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China

Sweetpotato, Ipomoea batatas(L.) Lam., is an important food crop worldwide. Large scale evaluation of sweetpotato germplasm for genetic diversity is necessary to determine the genetic relationships between them and effectively use them in the genetic improvement. In this study, the genetic diversity of 617 sweetpotato accessions, including 376landraces and 162 bred varieties from China and 79 introduced varieties from 11 other countries, was assessed using 30 simple sequence repeat(SSR) primer pairs with high polymorphism. Based on the population structure analysis,these sweetpotato accessions were divided into three groups, Group 1, Group 2 and Group 3, which included 228, 136and 253 accessions, respectively. Consistent results were obtained by phylogenic analysis and principal coordinate analysis(PCoA). Of the three groups, Group 2 showed the highest level of genetic diversity and its accessions were mainly distributed in low-latitude regions. The accessions from South China exhibited the highest level of genetic diversity, which supports the hypothesis that Fujian and Guangdong were the first regions where sweetpotato was introduced to China. Analysis of molecular variance(AMOVA) indicated significant genetic differentiations between the different groups, but low levels of genetic differentiation existed between the different origins and accession *** results provide valuable information for the better utilization of these accessions in sweetpotato breeding.

关键词： sweetpotato genetic diversity population structure SsR

Overexpression of IbSnRK1 enhances nitrogen uptake and carbon assimilation in transgenic sweetpotato

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Journal of Integrative Agriculture 2018年第2期17卷 296-305页

作者： REN Zhi-tong ZHAO Hong-yuan HE Shao-zhen ZHAI Hong ZHAO Ning liu qing-chang Key Laboratory of Sweetpotato Biology and Biotechnology Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/China Agricultural University

Nitrogen is an important nutrient for plant development. Nitrogen and carbon metabolisms are tightly linked to physiological functions in plants. In this study, we found that the IbSnRK1 gene was induced by Ca（NO3）2. Its overexpression enhanced nitrogen uptake and carbon assimilation in transgenic sweetpotato. After Ca（^15NO3）2 treatment, the -（15）N atom excess, -（15）N and total N content and nitrogen uptake efficiency（NUE） were significantly increased in the roots, stems, and leaves of transgenic plants compared with wild type（WT） and empty vector control（VC）. After Ca（NO3）2 treatment, the increased nitrate N content, nitrate reductase（NR） activity, free amino acid content, and soluble protein content were found in the roots or leaves of transgenic plants. The photosynthesis and carbon assimilation were enhanced. These results suggest that the IbSnRK1 gene play a important role in nitrogen uptake and carbon assimilation of sweetpotato. This gene has the potential to be used for improving the yield and quality of sweetpotato.

关键词： carbon assimilation IbSnRK1 nitrogen uptake sweetpotato

Overexpression of IbMIPS1 gene enhances salt tolerance in transgenic sweetpotato

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Journal of Integrative Agriculture 2016年第2期15卷 271-281页

作者： WANG Fei-bing ZHAI Hong AN Yan-yan SI Zeng-zhi HE Shao-zhen liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of EducationChina Agricultural University

Myo-inositol-1-phosphate synthase（MIPS） is a key rate limiting enzyme in the de novo biosynthesis of myo-inositol in *** the present study,the IbMIPS1 gene was introduced into sweetpotato cultivar Xushu 18 and the transgenic plants exhibited significantly enhanced salt tolerance compared with the wild-type（WT）.Overexpression of IbMIPSI up-regulated the salt stress responsive genes,including myo-inositol monophosphatase（MIPP）,pyrroline-5-carboxylate synthase（P5CS）,pyrroline-5-carboxylate reductase（P5CR）,psbA,phosphoribulokinase（PRK）,and superoxide dismutase（SOD） genes,under salt *** and proline content,SOD and photosynthesis activities were significantly increased,whereas malonaldehyde（MDA） and H_2O_2 contents were significantly decreased in the transgenic *** findings suggest that the IbMIPS1 gene may enhance salt tolerance of sweetpotato by regulating the expression of salt stress responsive genes,increasing the content of inositol and proline and enhancing the activity of photosynthesis.

关键词： IbMIPS1 overexpression salt tolerance sweetpotato

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新疆焉耆垦区防护林主要树种适宜性评价研究

西北林学院学报 2024年第4期39卷 189-199页

作者：张英凡楚光明刘长青王小兵王俊刚杨振安吕露雨石河子大学农学院新疆石河子832000 新疆生产建设兵团林业和草原资源监测中心新疆乌鲁木齐830000 新疆生产建设兵团第二师林业工作管理站新疆铁门关841007 石河子大学理学院新疆石河子832000

从焉耆垦区生态环境建设和防护林体系保护的实际需求出发,通过林地资源监测和实地调查,以树种的生态适应性、生态功能、综合效益和观赏价值作为防护林树种选择的主要评价准则,选取抗寒能力、抗旱能力、抗蛀干害虫能力、防风能力、经济... 详细信息

从焉耆垦区生态环境建设和防护林体系保护的实际需求出发,通过林地资源监测和实地调查,以树种的生态适应性、生态功能、综合效益和观赏价值作为防护林树种选择的主要评价准则,选取抗寒能力、抗旱能力、抗蛀干害虫能力、防风能力、经济效益等15个评价因子作为指标。利用层次分析法建立3级评价体系,运用多目标线性加权和法得出该区域64种主要防护林树种的树种适应性评价的总体得分值(Y),并根据该数值对防护林树种的适宜性进行等级划分。分析得到,Ⅰ级(Y≥4.0)适宜性优良的树种有泓森槐、胡杨、新疆杨等8种,Ⅱ级(3.6≤Y<4.0)适宜性较好的树种有刺槐、沙枣、山楂、美国黑核桃等19种,Ⅲ级(3.2≤Y<3.6)适宜性一般的树种有樟子松、大果榛子、臭椿、苹果等19种,Ⅳ级(2.8≤Y<3.2)适宜性较差的树种有梣叶槭、白榆、垂柳等13种,Ⅴ级(Y<2.8)适宜性极差的树种有箭杆杨、黑杨、竹柳等5种。

关键词：树种选择生态经济型抗蛀干害虫型层次分析法防护林评价

维普期刊数据库博看期刊

Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

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Journal of Integrative Agriculture 2013年第3期12卷 414-419页

作者： ZHAO Ning ZHAI Hong YU Xiao-xia liu Zhe-sheng HE Shao-zhen LI Qiang MA Dai-fu liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/China Agricultural University Xuzhou Sweetpotato Research Center

Sequence-related amplification polymorphism （SRAP） markers closely linked to stem nematode resistance gene were developed in sweetpotato, lpomoea batatas （L.） Lam. Using bulked segregant analysis （BSA）, 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781xsusceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nspl, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.

关键词： Ipomoea batatas (L.) Lam. SRAP marker stem nematode

Characterization of salt tolerance and Fusarium wilt resistance of a sweetpotato mutant

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Journal of Integrative Agriculture 2017年第9期16卷 1946-1955页

作者： ZHANG Huan ZHANG Qian WANG Yan-nan LI Yan ZHAI Hong liu qing-chang HE Shao-zhen Key Laboratory of Sweetpotato Biology and Biotechnology Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop He terosis and UtilizationMinistry of Education/China Agricultural UniversityBeijing 100193P.R.China

The variant LM1 was previously obtained using embryogenic cell suspension cultures of sweetpotato variety Lizixiang by gamma-ray induced mutation, and then its characteristics were stably inherited through six clonal generations, thus this mutant was named LM1. In this study, systematic characterization of salt tolerance and Fusarium wilt resistance were performed between Lizixiang and mutant LM1. LM1 exhibited significantly higher salt tolerance compared to Lizixiang. The content of proline and activities of superoxide dismutase（SOD） and photosynthesis were significantly increased, while malonaldehyde（MDA） and H_2O_2 contents were significantly decreased compared to that of Lizixiang under salt stress. The inoculation test with Fusarium wilt showed that its Fusarium wilt resistance was also improved. The lignin, total phenolic, jasmonic acid（JA） contents and SOD activity were significantly higher, while H_2O_2 content was significantly lower in LM1 than that in Lizixiang. The expression level of salt stress-responsive and disease resistance-related genes was significantly higher in LM1 than that in Lizixiang under salt and Fusarium wilt stresses, respectively. This result provides a novel and valuable material for improving the salt tolerance and Fusarium wilt resistance of sweetpotato.

关键词： Fusarium wilt resistance mutant salt tolerance sweetpotato