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Seedling Petri-dish inoculation method:A robust,easy-to-use and reliable assay for studying plant-Ralstonia solanacearum interactions

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Journal of Integrative Agriculture 2023年第12期22卷 3709-3719页

作者： CAO Peng CHEN Jia-lan LI Ning-ning ZHANG Shuang-xi WANG Rong-bo LI Ben-jin liu pei-qing AN Yu-yan ZHANG Mei-xiang National Engineering Laboratory for Endangered Medicinal Resource Development in Northwest China/Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry of Ministry of Education College of Life SciencesShaanxi Normal UniversityXi’an 710119P.R.China Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095P.R.China Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests Institute of Plant ProtectionFujian Academy of Agricultural SciencesFuzhou 350013P.R.China

Ralstonia solanacearum causes a lethal bacterial wilt disease in many crops,leading to huge losses in crop production every *** of *** interactions will aid to develop efficient strategies to control the *** a soilborne pathogen,*** naturally infects plants via roots.A huge limitation in studying *** interactions is the large variation of *** infection assay due to the variable soil conditions and uneven inoculum ***,we developed a robust and reliable Petri-dish inoculation method which allows consistent and stable infection in young plant *** method is easy to use,takes about only 10 days from seed germination to the completion of inoculation assay,and requires less inoculum of bacteria as well as growth chamber *** proved the efficacy of the seedling Petri-dish inoculation method by analyzing plant defense primed by molecular patterns,resistance of defense-related plant mutants,and virulence of *** ***,we demonstrated that the seedling Petri-dish inoculation method can be applied to other host plants such as tobacco and has great potential for high-throughput screening of resistant plant germplasms to bacterial wilt in the future.

关键词： bacterial wilt Ralstonia solanacearum Petri-dish inoculation method virulence resistance defense priming

U-SEQUENCE AND PERIOD-TRIPLING PHENOMENA IN A FORCED NONLINEAR CSCILLATOR

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Chinese Physics Letters 1985年第5期2卷 213-216页

作者： WU Shu-xian pei liu-qing GUO Fen Department of Electronics Beijing Normal UniversityBeijing

Experiments on a nonlinear oscillator reveal the same periodic structure as that in one-dimensional unimodal maps and a simple method is found to assign words from a symbolic dynamics of two letters to the periods observed in *** paper gives plentiful patterns of U-sequence,including period-tripling patterns R*RL,RL*RL and RL^(2)**** analysis gives a confirmation of the patterns.

关键词： patterns. assign symbolic

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高拱坝挑跌流非碰撞水垫塘消能形式研究

水利学报 2010年第7期40卷 841-848页

作者：刘沛清许唯临流体力学教育部重点实验室北京航空航天大学北京100191 四川大学水力学与山区河流开发保护国家重点实验室四川成都610065

在峡谷区修建高坝工程,坝身高水头、大泄量的消能防护是关键技术难题之一。本文通过模型试验和数值模拟,研究了收缩式消能工的作用和分层射流在水垫塘内流动特征与消能机理,论述了坝身分层、多孔、分散泄洪的可能型式及其优缺点。为避... 详细信息

在峡谷区修建高坝工程,坝身高水头、大泄量的消能防护是关键技术难题之一。本文通过模型试验和数值模拟,研究了收缩式消能工的作用和分层射流在水垫塘内流动特征与消能机理,论述了坝身分层、多孔、分散泄洪的可能型式及其优缺点。为避免射流水舌碰撞分散引起的溅水雾化问题,通过对坝身表、深孔的不同布置形式,创造性地提出表孔、深孔水舌分层入水非碰撞布局的水垫塘消能方案,和采用收缩式消能工,使表、深孔在横向交错布置,在立面分层泄流,深孔水舌穿过表孔水舌之间的空隙处射出,形成交错入水非碰撞格局的水垫塘消能方案。

关键词：射流碰撞消能型式

非共面近距耦合鸭式布局鸭翼展向脉冲吹气增升特性

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实验流体力学 2011年第4期25卷 37-41页

作者：刘杰刘沛清曹硕成都飞机设计研究所成都610041 北京航空航天大学流体力学研究所北京100191

对40°前缘后掠角的主翼和40°前缘后掠角的鸭翼所构成的近距耦合鸭式布局简化模型进行了风洞测力、测压实验,系统研究了鸭翼展向脉冲吹气的增升效果,给出脉冲吹气频率以及脉冲宽度与布局升力之间的变化关系。测力结果表明,鸭翼展向吹... 详细信息

对40°前缘后掠角的主翼和40°前缘后掠角的鸭翼所构成的近距耦合鸭式布局简化模型进行了风洞测力、测压实验,系统研究了鸭翼展向脉冲吹气的增升效果,给出脉冲吹气频率以及脉冲宽度与布局升力之间的变化关系。测力结果表明,鸭翼展向吹气提高了该布局在大迎角时的升力,延迟了失速。测压结果表明,鸭翼展向脉冲吹气改善了中大迎角时主翼翼面流态,增加了翼面吸力峰值,延缓了涡的破裂。这说明利用鸭翼展向脉冲吹气涡控技术,可以直接改善鸭翼流场,继而间接改善主翼流场。

关键词：非共面鸭式布局展向吹气间接涡控制技术测力测压

Exploring the Variety of Meaning in the Main Characters of Frankenstein

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海外英语 2016年第20期 151-152页

作者：刘佩晴国防科学技术大学

Frankenstein is the representative work of Mary Shelley. In this work, there are three main characters: Victor Frankenstein, the Creature and Walton. This essay will explore the variety of meaning of the three main ch... 详细信息

关键词： Frankenstein Mary Shelley

中等后掠角三角翼前缘双涡结构的形成机理数值研究

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空气动力学学报 2012年第6期30卷 767-771页

作者：刘杰刘沛清闫指江北京航空航天大学流体力学教育部重点实验室北京100191 成都飞机设计研究所四川成都610091

采用数值计算的方法,对前缘后掠角为50°的三角翼布局在不同迎角下的绕流结构及其演化进行了研究。在不同迎角下,计算的压力分布与实验结果吻合较好。对数值计算的流场结构分析发现:对于中等后掠角三角翼,在一定的雷诺数和迎角下,在机... 详细信息

采用数值计算的方法,对前缘后掠角为50°的三角翼布局在不同迎角下的绕流结构及其演化进行了研究。在不同迎角下,计算的压力分布与实验结果吻合较好。对数值计算的流场结构分析发现:对于中等后掠角三角翼,在一定的雷诺数和迎角下,在机翼翼面上会出现前缘双涡结构。其形成取决于三角翼前缘附近三维分离产生的自由剪切层强度和涡轴方向的速度分量之间的大小关系。一般而言,当涡轴的速度大到足以将剪切层涡量带走时,在机翼前缘常常会表现为单涡结构;反之将导致剪切层分叉,由此形成双涡结构。

关键词：双涡结构三角翼旋涡流场

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地效飞行器地面巡航气动性能数值模拟及分析

航空学报 2006年第1期27卷 16-22页

作者：屈秋林刘沛清北京航空航天大学航空科学与工程学院北京100083

采用有限体积方法求解定常可压缩流动的质量加权平均N-S方程和标准k-ε湍流模型,模拟地效飞行器地面巡航全机流场。计算结果给出了地效飞行器气动性能随飞行高度和迎角的变化规律;采用镜像法理想涡模型分析了地面效应对翼尖涡位置和气... 详细信息

采用有限体积方法求解定常可压缩流动的质量加权平均N-S方程和标准k-ε湍流模型,模拟地效飞行器地面巡航全机流场。计算结果给出了地效飞行器气动性能随飞行高度和迎角的变化规律;采用镜像法理想涡模型分析了地面效应对翼尖涡位置和气流下洗角的影响;详细阐明了地面效应对诱导阻力的影响规律。

关键词：地效飞行器翼尖涡下洗角诱导阻力数值模拟理想涡模型

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无压瞬变流的改进断波法及其应用

长江科学院院报 2001年第3期18卷 3-6页

作者：王刚刘沛清北京航空航天大学流体力学研究所北京100083

对动力渠道瞬变流计算中的断波法进行了改进 ,一是构造关联方程组 ,二是采用新的迭代法 ,使不收敛的方程组在给定精度条件下可以进行迭代计算。该迭代法准确性高且迭代速度非常快。用该方法对金水街水电站在甩全负荷的情况下动力渠道中... 详细信息

对动力渠道瞬变流计算中的断波法进行了改进 ,一是构造关联方程组 ,二是采用新的迭代法 ,使不收敛的方程组在给定精度条件下可以进行迭代计算。该迭代法准确性高且迭代速度非常快。用该方法对金水街水电站在甩全负荷的情况下动力渠道中的涌波过程进行了计算 ,并与原电站设计进行了比较 ,结果表明改进断波法是切实可行的。

关键词：动力明渠瞬变流断波法数学模型

Comparative proteomic analysis of differentially expressed proteins between K562 and K562/ADM cells

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Chinese Medical Journal 2008年第5期121卷 463-468页

作者： SHEN Shao-hua GU Long-jun liu pei-qing YE Xin CHANG Wei-shan LI Ben-shang Shanghai Xinhua Hospital Shanghai Children's Medical Center Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200127 China Laboratory of Pharmacology and Toxicology School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou Guangdong 510080 China Taian Central Hospital Taian Shandong 271000 China Vaccine and Infectious Disease Organization Saskatoon S7N5E3 Canada

Background Multidrug resistance to chemotherapeutic agents is an important clinical problem during the treatment of leukemia. The resistance process is multifactorial. To realize the total factors involved in multidrug resistance, we analyzed the differentially expressed proteins of K562 and K562/ADM cells and we investigated one of the up-regulated proteins （CRKL） using siRNA to determine its role in K562/ADM cells. Methods Altered protein expressions between K562/S （K562 ADM-sensitive cell line） and K562/ADM （K562 multidrug resistant cell line induced by adriamycin） were identified by 2D-DIGE coupled with mass spectrometry. Meanwhile, we confirmed the differential expression of CRKL and Stathmin in both K562 and K562/ADM cells by Western blot analysis. Furthermore, we used RNA interference to silence the CRKL gene expression. Results Among the 9 differentially expressed proteins, 3 were up-regulated in K562/ADM cells, while 6 were down-regulated in the K562/ADM cells compared with its parent cell line. The expression of CRKL was up-regulated significantly in K562/ADM cells, and it can be decreased by recombinant lentivirus. Moreover, the multidrug resistance of K562/ADM cells was efficiently reversed by silence of CRKL gene expression. Conclusions The data provided the differentially expressed proteins in K562 and its resistant cell line and highlights the power of 2D-DIGE for the discovery of resistance markers in cancer. We found CRKL may be a new protein involved in the multidrug resistanse of leukaemia cells.

关键词： multidrug resistance leukemia adriamycin proteomics