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In vitro study of micro-dystrophin gene-modified mesenchymal stem cells

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Neural Regeneration Research 2010年第7期5卷 496-501页

作者： Daidi Zhao Zhiyun Lian Libin liu Li luo Huiying Li Ju liu Hongyu Zhou Department of Neurology West China Hospital of Sichuan University Chengdu 610041 Sichuan Province China

BACKGROUND： Studies have shown that the transplantation of mesenchymal stem cells （MSCs） improves dystrophin expression in muscle cell membrane of mdx mice and plays a role in ameliorating sport injuries to the myocyte. In addition, dystrophin gene plasmid injection exhibits therapeutic effect in mdx mice. However, these two methods exhibit shortcomings, such as low rate of post-transplantation expression. Therefore, the present study determined the combinatorial effects of these two methods. OBJECTIVE： To transfect and observe effects of pSL139 plasmid carrying the micro-dystrophin gene into MSCs, as well as in vitro micro-dystrophin gene expression in transfected MSCs. DESIGN, TIME AND SETTING： A comparative, molecular biology study was performed at the Laboratory of Tissue Engineering, West China Medical Center, Sichuan University from March 2007 to February 2008. MATERIALS： The pSL139 plasmid was cloned and provided by the Department of Neurology, Washington University, USA. Lipofectamine 2000 was purchased from Invitrogen, USA. Mouse anti-human dystrophin N-based terminal monoclonal antibody was purchased from Chemicon, USA. METHODS： Differential velocity adherent technique and density gradient centrifugation were combined to separate and culture MSCs from C57/BL10 mice. The cells were induced to trans-differentiate into osteoblasts. Subsequently, the Lipofectamine 2000 method was used to mediate transfection of plasmid pSL139 into third generation MSCs. MAIN OUTCOME MEASURES： Semi-quantitative reverse transcription polymerase-chain reaction and immunofluorescence were respectively employed to detect micro-dystrophin mRNA and protein expressions in MSCs. RESULTS： At 48 hours after MSC transfection with plasmid pSL139, a 379-kb target band was observed by agarose gel electrophoresis. Immunofluorescence revealed micro-dystrophin expression up to 45%-55%. CONCLUSION： Micro-dystrophin mRNA and protein were highly expressed in pSL139-transfected MSCs, which provided

关键词： Duchenne muscular dystrophy mesenchymal stem cells micro-dystrophin gene modification

Liposomes as versatile agents for the management of traumatic and nontraumatic central nervous system disorders:drug stability,targeting efficiency,and safety

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Neural Regeneration Research 2025年第7期20卷 1883-1899页

作者： Mingyu Zhang Chunyu Xiang Renrui Niu Xiaodong He Wenqi luo Wanguo liu Rui Gu Department of Orthopedic Surgery China-Japan Union Hospital of Jilin UniversityChangchunJilin ProvinceChina

Various nanoparticle-based drug delivery systems for the treatment of neurological disorders have been widely ***,their inability to cross the blood–brain barrier hampers the clinical translation of these therapeutic *** are nanoparticles composed of lipid bilayers,which can effectively encapsulate drugs and improve drug delivery across the blood–brain barrier and into brain tissue through their targeting and ***,they can potentially treat traumatic and nontraumatic central nervous system *** this review,we outlined the common properties and preparation methods of liposomes,including thin-film hydration,reverse-phase evaporation,solvent injection techniques,detergent removal methods,and microfluidics ***,we comprehensively discussed the current applications of liposomes in central nervous system diseases,such as Alzheimer's disease,Parkinson's disease,Huntington's disease,amyotrophic lateral sclerosis,traumatic brain injury,spinal cord injury,and brain *** studies related to liposomes are still in the laboratory stage and have not yet entered clinical ***,their application as drug delivery systems in clinical practice faces challenges such as drug stability,targeting efficiency,and ***,we proposed development strategies related to liposomes to further promote their development in neurological disease research.

关键词： Alzheimer's disease amyotrophic lateral sclerosis brain tumors central nervous system Huntington's disease liposome drug delivery neurological disorders Parkinson's disease spinal cord injury traumatic brain injury

Stability of gyrotheodolite

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中国有色金属学会会刊：英文版 2005年第S1期15卷 17-21页

作者： liu Xiao-sheng, luo Ren-xiu (Jiangxi University of Science and Technology, Ganzhou 341000, China) Jiangxi Univ Sci & Technol Ganzhou 341000 Peoples R China

The checking methods of gyrotheodolite stability were proposed. The main factors estimating the stability of gyrotheodolite were determined. They are the stability of the mean value of oscillating, zero position and the quality of magnet shield. The evaluation mathematical model for gyrotheodolite stability was established based upon the principle of fuzzy mathematics and an effective method of quantitative analysis of the gyrotheodolite stability was presented. By taking JT15 and other gyrotheodolites for examples, the effect of voltage and frequency on the rotational speed of gyro-motor and on the stability of the mean value of oscillating of gyro, the influence of temperature, magnetic field, vibration on stability of gyrotheodolite was studied. At last some concrete measurements for enhancing the stability of orientating precision of the gyro were suggested.

关键词： gyrotheodolite stability orientation mean value of oscillating zero position

Genetic variation of Porphyra yezoensis by using AFLP1

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Acta Oceanologica Sinica 2003年第3期22卷 453-457页

作者： Rui Yang, Biqian liu, Qijun luo, Yajun Wang, Jiamei Bao Marine Biotechnology Institution, Ningbo University,Ningbo 315211,China.E-mail:yangrui @ *** Ningbo Univ Marine Biotechnol Inst Ningbo 315211 Peoples R China

Genetic variation of 11 lines of Porphyra yezoensis from the coastline of Kagoshima of Japan, Qingdao, Nantong, Putuo and Nanji Islands of China were studied by using amplified fragment length polymorphism (AFLP). 778 bands were obtained with AFLP analysis of 16 primer combinations, among which 15 were unique, about 98.07% were polymorphic. The AFLP data showed that the closest genetic distance was 0 - 180 between two Kagoshima samples, and the farthest one was 0.397 between Kagoshima No. 1 and Nantong No - 9 line. The genetic distance showed that the variation was within the inner species scope. Neighbor-joining cluster and UPGMA cluster indicated that samples from Kagoshima and Qingdao were with high similarity and either with the samples of Nantong, Putuo and Nanji Islands. P. yezoensis in China shared high genetic diversity, and the genetic distance showed positive correlation with the geographic distance.

关键词： Porphyra yezoensis AFLP genetic variation

A trust-based method for selection of contractors in construction industry

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International Journal of Technology Management 2013年第2期 122-126页

作者： Jing XU Yanxin liu Lanfang luo Southwest Jiaotong University Chengdu 610031 E R. China

This paper presents a method for construction contractor selection based on trust building. By classifying criteria into three types of trust and applying gray correlation analysis, the presented method concerns multi-criteria for evaluation other than price, selects trust- and selection-related criteria, provides approaches for indicator quantification and determines the contractor on the basis of gray correlation degree.

关键词： Trust contractor selection gray correlation analysis

Safety and efficacy of intravenous esmolol before prospective electrocardio- gram-triggered high-pitch spiral acquisition for computed tomography coro- nary angiography

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Journal of Geriatric Cardiology 2014年第1期11卷 39-43页

作者： Jin-Da WANG Hua-Wei ZHANG Qian XIN Jun-Jie YANG Zhi-Jun SUN Hong-Bin liu LianCHEN luo-Shan DU Yun-Dai CHEN Department of Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China

Background In order to acquire a high quality image with a low radiation dose, prospective electrocardiogram （ECG）-triggered computed tomography coronary angiography （CTCA） requires a stable heart rate （HR）〈 65 beats/min. Esmolol has the advantage of reduc-ing HR. The objective of this article is to assess the value of intravenous esmolol treatment before prospective ECG-tr/ggered high-pitch spiral acquisition for CTCA. Methods From March 2013 to June 2013, 313 patients underwent prospective ECG-triggered CTCA. Two hundred and thirty two of them received esmolol before angiography. We retrospectively analyzed clinical characteristics, esmolol dose, radiation exposure dose, and the change in HR and blood pressure in these 232 patients. Results A total of 232 patients with a HR 〉 65 beats/rain before CTCA examination received intravenous esmolol treatment （mean dose of 57.26±15.39 rag）, The mean initial HR （HR1）, slowest HR （HR2）, and the HR 30 min after HR2 （HR3） were 75.06± 5.59, 60.75 ±4.00, and 75.54 ± 5.96 beats/min, respectively （HR1 vs. HR2, P 〈 0.0001; HRI vs. HR3, P = 0.377）. The mean time from esmolol administration to HR2 was 24.25 ± 4.97 s and the mean effective radiation dose was 2.28 ± 0.02 mSv. Conclusions HR could be rapidly controlled at an optimum level with intravenous esmolol before prospective ECG-triggered high-pitch spiral acquisition for CTCA. Consequently, the patients received a very low radiation dose.

关键词： Esmolol Electrocardiogram Coronary angiography Heart rate

Study of Laser Ocular Effect and Damage

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美国中华健康卫生杂志 2005年第3期8卷 66-68页

作者： liu Haifeng luo Zhenkun ZHANG Jian Institute of Radiation Medicine Academy of Military Medical Sciences Beijing China

Clinical and endoscopic analysis of gastric Dieulafoy’s lesion

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World Journal of Gastroenterology 2010年第5期16卷 631-635页

作者： Yi-Juan Ding,Liang Zhao,Jun liu,He-Sheng luo,Department of Gastroenterology,Renmin Hospital of Wuhan University, Jiefang Road 238,Wuhan 430060,Hubei Province,China Department of Gastroenterology Renmin Hospital of Wuhan University Jiefang Road 238 Wuhan 430060 Hubei Province China. airman-zhao@***

AIM:To investigate the incidence,location,clinical presentation,diagnosis and effectiveness of endoscopic treatment of gastric Dieulafoy's lesion(DL)in China. METHODS:All patients who received emergency upper gastrointestinal(GI)endoscopy due to gastric DL from February 2000 to August 2008 at GI endoscopy center of Renmin Hospital of Wuhan University were included in this *** clinical presentation,medical history,location and characteristics of DL methods and effectiveness of therapy of patients with DL were retrospectively analysed by chart ***-term follow-up data were collected at outpatient clinics or telephone interviews. RESULTS:Fifteen patients were diagnosized with DL,which account for 1.04%of the source of bleed- ing in acute non-variceal upper GI *** comorbidities were found in one patient with hypertension and diabetic *** or combined therapy with hemoclip produced primary hemostasis in 92.8%(13/14) of patients. CONCLUSION:DL is uncommon but life-threatening in *** proved to be safe and effective in controlling bleeding from DL.

关键词： Gastric Dieulafoy’s lesion Clinical analysis Endoscopic analysis

THE RESEARCH ON MODEL OF PROBABILITY DAMAGE TOLERANCE ANALYSIS

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Chinese Journal of Aeronautics 1993年第4期 253-260页

作者： liu Wen-ting,luo Yi,Tong Ming-bo (Beijing University of Aeronautics and Astronautics,Beijing,China,100083)

A new model of probability damage tolerance analysis is presented. It is composed ofua-t" random crack growth model and reliability analysis model which are based onthe life-failure criterion. The former establishes distribution function oflife for arbitrarycrack length by using reasonable "a-t" relation and statistically analysing life data forarbitrary given crack length. The latter indicates that safe reliability R corresponding togiven life tis probability of random critical life exceeding t. This new model can remedythe weakness of previous analysis model of probability damage tolerance, which is composedof "da/dt" random process model and "a-a" （or "K-K" ） reliabilityinterference model, and furthermore, it is very convenient to engineering use.

关键词： THE RESEARCH ON MODEL OF PROBABILITY DAMAGE TOLERANCE ANALYSIS