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MicroRNA-27a-mediated repression of cysteine-rich secretory protein 2 translation in asthenoteratozoospermic patients

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Asian Journal of Andrology 2017年第5期19卷 591-595页

作者： jun-Hao Zhou qi-Zhao Zhou jian-Kun yang Xiao-Ming Lyu jun Bian wen-Bin guo Zi-jian Chen Ming Xia hui Xia Tao qi xin li Cun-Dong liu Department of Urology The Third Affiliated Hospital of Southern Medical University Guangzhou China Laboratory Medical Center The Third Affiliated Hospital of Southern Medical University Guangzhou China Laboratory Medicine Center Nanfang Hospital Southern Medical University Guangzhou China Cancer Research Institute Southern Medical University Guangzhou China

Cysteine-rich secretory protein 2 （CRISP2） is an important protein in spermatozoa that plays roles in modulating sperm flagellar motility, the acrosome reaction, and gamete fusion. Spermatozoa lacking CRISP2 exhibit low sperm motility and abnormal morphology. However, the molecular mechanisms underlying the reduction of CRISP2 in asthenoteratozoospermia （ATZ） remain unknown. In this study, low expression of CRISP2 protein rather than its mRNA was observed in the ejaculated spermatozoa from ATZ patients as compared with normozoospermic males. Subsequently, bioinformatic prediction, luciferase reporter assays, and microRNA-27a （miR-27a） transfection experiments revealed that miR-27a specifically targets CRISP2 by binding to its 3＇ untranslated region （3＇-UTR）, suppressing CRISP2 expression posttranscriptionally. Further evidence was provided by the clinical observation of high miR-27a expression in ejaculated spermatozoa from ATZ patients and a negative correlation between miR-27a expression and CRISP2 protein expression. Finally, a retrospective follow-up study supported that both high miR-27a expression and low CRISP2 protein expression were associated with low progressive sperm motility, abnormal morphology, and infertility. This study demonstrates a novel mechanism responsible for reduced CRISP2 expression in ATZ, which may offer a potential therapeutic target for treating male infertility, or for male contraception.

关键词： asthenoteratozoospermia cysteine-rich secretory protein 2 male infertility microRNA-27a

Towards the full realization of the RIBLL2 beam line at the HIRFL-CSR complex

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Science Bulletin 2018年第2期63卷 78-80页

作者： Bao-hua Sun jian-Wei Zhao Xue-Heng Zhang li-Na Sheng zhi-Yu Sun Isao Tanihata Satoru Terashima Yong Zheng li-hua Zhu li-Min Duan liu-Chun He Rong-jiang hu Guang-Shuai li wen-jian lin Wei-Ping lin Chuan-Ye liu Zhong liu Chen-Gui Lu xin-wen Ma li-jun Mao Yi Tian Feng wang Meng wang Shi-Tao wang Jia-wen Xia Xiao-Dong Xu hu-Shan Xu zhi-guo Xu jian-Cheng yang Da-Yu Yin You-Jin Yuan wen-Long Zhan Yu-hu Zhang Xiao-Hong Zhou School of Physics and Nuclear Energy Engineering Beihang University Beijing 100191 China International Research Center for Nuclei and Particles in the Cosmos Beijing 100191 China Institute of Modern Physics Chinese Academy of Sciences Lanzhou 730000 China

More than 99%of the mass in the visible universe—the material that makes up ourselves,our planet,stars—is in the atomic *** the matter has existed for billions of years,only over the past few decades have we had the tools and the knowledge necessary to get a basic understanding of the structure and dynamic of *** physicists around the world have made tremendous strides by initiating a broad range of key

关键词： HIRFL-CSR 横梁建筑群排队原子核物理学家试验性世界

Design and tests of the prototype a beam monitor of the CSR external target experiment

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Nuclear Science and Techniques 2022年第3期33卷 137-151页

作者： hu-lin wang Zhen wang Chao-Song Gao jian-Wei liao Xiang-Ming Sun hai-Bo yang Cheng-xin Zhao jun liu Peng Ma Zi-li li Bi-hui You Ping yang Di guo Le Xiao Dong-liang Zhang Yue-Zhao Zhang Sheng Dong Wan-Han Feng Yu-xin qiao Zheng-Yu hu qing-wen Ye Zeng-Tao guo Guang-Ming huang Feng liu PLAC Key Laboratory of Quark and Lepton Physics(MOE)Central China Normal UniversityWuhan 430079China hubei Provincial Engineering Research Center of Silicon Pixel Chip&Detection Technology Wuhan 430079China School of Physics and Electronic Science Guizhou Normal UniversityGuiyang 550001China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China

A prototype beam monitor was designed to provide tracking information for heavy-ion projectiles for the cool storage ring(CSR)external target experiment(CEE)at the Heavy Ion Research Facility in Lanzhou(HIRFL).High granularity and direct charge sensing are the main features of this *** measures the beam position in a two-dimensional(2D)plane transverse to the beam direction on an event-by-event *** current design consists of two field cages inside a single vessel that operates independently and has electrical drift fields in orthogonal *** tests of the prototype were performed using a^(241)Am a *** results show that a spatial resolution of less than 40μm and a time resolution of less than 600 ns can be achieved.

关键词： CEE Beam monitor Heavy ion Topmetal sensor Tracking Field cage

Protective efficacy of an H5/H7 trivalent inactivated vaccine(H5-Re13,H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses

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Journal of Integrative Agriculture 2022年第7期21卷 2086-2094页

作者： ZENG Xian-ying HE xin-wen MENG Fei MA qi wang Yan BAO Hong-mei liu Yan-jing DENG guo-hua SHI jian-zhong li Yan-bing TIAN guo-bin CHEN hua-lan State Key Laboratory of Veterinary Biotechnology National Poultry Laboratory Animal Resource CenterHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.***.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.***.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.***.4.4 b HA gene, an H5 N6 virus bearing the clade 2.***.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.***.4.4 h or 2.***.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature.

关键词： avian influenza H5/H7 trivalent vaccine H5-Re13 H5-Re14 H7-Re4 strains protective efficacy chickens ducks and geese

Transarterial chemoembolization with PD-(L)1 inhibitors plus molecular targeted therapies for hepatocellular carcinoma(CHANCE001)

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Signal Transduction and Targeted Therapy 2023年第3期8卷 1198-1207页

作者： hai-Dong Zhu hai-liang li Ming-Sheng huang Wei-Zhu yang guo-wen Yin Bin-Yan Zhong jun-hui Sun zhi-Cheng Jin jian-jian Chen Nai-jian Ge wen-Bin Ding wen-hui li Jin-hua huang Wei Mu Shan-zhi Gu Jia-Ping li hui Zhao Shu-Wei wen Yan-Ming Lei Yu-Sheng Song Chun-wang Yuan Wei-Dong wang Ming huang Wei Zhao jian-Bing Wu Song wang Xu Zhu jian-jun Han Wei-xin Ren Zai-Ming Lu wen-Ge xing Yong Fan hai-Lan lin Zi-Shu Zhang guo-hui Xu wen-Hao hu qiang Tu Hong-Ying Su Chuan-Sheng Zheng Yong Chen Xu-Ya Zhao Zhu-Ting Fang qi wang Jin-Wei Zhao Ai-Bing Xu jian Xu qing-hua Wu huan-Zhang Niu jian wang Feng Dai Dui-Ping Feng qing-Dong li Rong-Shu Shi Jia-Rui li Guang yang hai-Bin Shi jian-Song Ji Yu-E liu Zheng Cai Po yang yang Zhao Xiao-li Zhu li-Gong Lu Gao-jun Teng Center of Interventional Radiology&Vascular Surgery Department of RadiologyZhongda HospitalMedical SchoolSoutheast UniversityNanjing 210009China Department of Minimally invasive Intervention The Affiliated Cancer Hospital of Zhengzhou UniversityZhengzhou 450008China Department of Interventional Radiology the Third Affiliated HospitalSun Yat-Sen UniversityGuangzhou 510630China Department of Interventional Radiology Union Hospital of Fujian Medical UniversityFuzhou 350001China Department of Interventional Radiology Jiangsu Cancer Hospital&Jiangsu Institute of Cancer Research&The Affiliated Cancer Hospital of Nanjing Medical UniversityNanjing 210009China Department of Interventional Radiology The First Affiliated Hospital of Soochow UniversitySoochow UniversitySuzhou 215006China Hepatobiliary and Pancreatic Interventional Treatment Center Division of Hepatobiliary and Pancreatic SurgeryThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Department of Interventional Radiology Eastern Hospital of Hepatobiliary SurgeryNavy Medical University(Second Military Medical University)Shanghai 200438China Department of Interventional Radiology Nantong First People’s HospitalNantong 226001China Department of Interventional Radiology Yancheng Third People’s HospitalYancheng 224008China Department of Minimally Invasive Interventional Therapy Sun YatSen University Cancer CenterGuangzhou 510060China Department of Vascular Surgery Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China. Department of Interventional Radiology Hunan Cancer HospitalChangsha 410031China Department of Interventional Oncology The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Department of Interventional Radiology The Hospital of Nantong UniversityNantong 226001China Department of Interventional Therapy Shanxi Tumor HospitalTaiyuan 030001China Department of Interventional Radiology Tibet Autonomous Region People’s

There is considerable potential for integrating transarterial chemoembolization(TACE),programmed death-(ligand)1(PD-[L]1)inhibitors,and molecular targeted treatments(MTT)in hepatocellular carcinoma(HCC).It is necessary to investigate the therapeutic efficacy and safety of TACE combined with PD-(L)1 inhibitors and MTT in real-world *** this nationwide,retrospective,cohort study,826 HCC patients receiving either TACE plus PD-(L)1 blockades and MTT(combination group,n=376)or TACE monotherapy(monotherapy group,n=450)were included from January 2018 to May *** primary endpoint was progression-free survival(PFS)according to modified *** secondary outcomes included overall survival(OS),objective response rate(ORR),and *** performed propensity score matching approaches to reduce bias between two *** matching,228 pairs were included with a predominantly advanced disease *** PFS in combination group was 9.5 months(95%confidence interval[CI],8.4-11.0)versus 8.0 months(95%CI,6.6-9.5)(adjusted hazard ratio[HR],0.70,P=0.002).OS and ORR were also significantly higher in combination group(median OS,19.2[16.1-27.3]vs.15.7 months[13.0-20.2];adjusted HR,0.63,P=0.001;ORR,60.1%vs.32.0%;P<0.001).Grade 3/4 adverse events were observed at a rate of 15.8%and 7.5%in combination and monotherapy groups,*** results suggest that TACE plus PD-(L)1 blockades and MTT could significantly improve PFS,OS,and ORR versus TACE monotherapy for Chinese patients with predominantly advanced HCC in real-world practice,with an acceptable safety profile.

关键词： hepatocellular targeted matching

Stepped-up development of accelerator mass spectrometry method for the detection of ^(60)Fe with the HI-13 tandem accelerator

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Nuclear Science and Techniques 2024年第4期35卷 136-143页

作者： yang Zhang Sheng-Quan Yan Ming He qing-Zhang Zhao wen-hui Zhang Chao-xin Kan jian-Ming Zhou Kang-Ning li Xiao-Fei wang jian-Cheng liu Zhao-hua Peng Zhuo liang Ai-ling li jian Zheng qi-wen Fan Yun-Ju li You-Bao wang zhi-Hong li yang-Ping Shen Ding Nan Wei Nan Yu-qiang Zhang Jia-Ying-Hao li jun-wen Tian jiang-lin Hou Chang-xin guo zhi-Cheng Zhang Ming-Hao Zhu Yu-wen Chen Yu-Chen jiang Tao Tian Jin-Long Ma Yi-hui liu Jing-Yu Dong Run-Long liu Mei-Yue-Nan Ma Yong-Shou Chen Wei-Ping liu Bing guo China Institute of Atomic Energy P.O.Box 275(10)Beijing 102413China College of Science Southern University of Science and TechnologyShenzhen 518055China School of Physics Xi’an Jiaotong UniversityXi’an 710049China

The Moon provides a unique environment for investigating nearby astrophysical events such as *** samples retain valuable information from these events,via detectable long-lived“fingerprint”radionuclides such as^(60)*** this work,we stepped up the development of an accelerator mass spectrometry(AMS)method for detecting^(60)Fe using the HI-13tandem accelerator at the China Institute of Atomic Energy(CIAE).Since interferences could not be sufficiently removed solely with the existing magnetic systems of the tandem accelerator and the following Q3D magnetic spectrograph,a Wien filter with a maximum voltage of±60 kV and a maximum magnetic field of 0.3 T was installed after the accelerator magnetic systems to lower the detection background for the low abundance nuclide^(60)Fe.A 1μm thick Si_(3)N_(4) foil was installed in front of the Q3D as an energy *** particle detection,a multi-anode gas ionization chamber was mounted at the center of the focal plane of the ***,an^(60)Fe sample with an abundance of 1.125×10^(-10)was used to test the new AMS *** results indicate that^(60)Fe can be clearly distinguished from the isobar^(60)*** sensitivity was assessed to be better than 4.3×10^(-14)based on blank sample measurements lasting 5.8 h,and the sensitivity could,in principle,be expected to be approximately 2.5×10^(-15)when the data were accumulated for 100 h,which is feasible for future lunar sample measurements because the main contaminants were sufficiently separated.

关键词： Accelerator mass spectrometry Wien filter Isobar separation Supernovae Chang'e-5 lunar samples

SAPT:a synchrotron-based proton therapy facility in Shanghai

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Nuclear Science and Techniques 2023年第10期34卷 36-46页

作者： Man-Zhou Zhang De-Ming li li-Ren Shen hai-Rong Zhang zhi-ling Chen Han-wen Du Ming Gu Rui li De-Kang liu Yue-hu Pu jun-Feng Yu jian-Feng Chen Chu Chen Chun-Long guo Hao guo Ge-yang jiang zhi-qiang jiang lin Jin wen-Jing li Xiu-Fang li Ye lin Ming liu Yong-Hao liu Ya-Juan liu Ming Lv qing-Ru Mi lian-hua Ouyang Wei-guo Shi Hang Shu qi-Sheng Tang Kun wang zhi-Shan wang jun Wu Xiao-Bing Wu Jia-qiang Xu wen-Zhen Xu Chong-Xian Yin Cheng Yu Ren-Xian Yuan qi-Bing Yuan hai-Qun Zhang Miao Zhang wen-zhi Zhang li-Ying Zhao Wei-Min Zhou Shou-Xian Fang Xi-Dong Sun Zhen-Tang Zhao Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201204China APACTRON Particle Equipment Co. Ltd.Shanghai 201815China The Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

Because of its excellent dose distribution,proton therapy is becoming increasingly popular in the medical application of cancer treatment.A synchrotron-based proton therapy facility was designed and constructed in *** synchrotron,beam delivery system,and other technical systems were commissioned and reached their expected *** a clinical trial of 47 patients was finished,the proton therapy facility obtained a registration certificate from the National Medical Products *** characteristics of the accelerator and treatment systems are described in this article.

关键词： Proton therapy Synchrotron Slow extraction Gantry Beam delivery system

Three-dimensional architecture using hollow Cu/C nanofiber interpenetrated with MXenes for high-rate lithium-ion batteries

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Rare Metals 2023年第10期42卷 3378-3386页

作者： Jing-Chong liu lin-lin Ma Shuai li Lan-Lan Hou xin-Ran qi Yong-qiang wen guo-Ping hu Nüwang Yong Zhao Xiao-Xian Zhao Beijing Key Laboratory for Bioengineering and Sensing Technology Daxing Research InstituteSchool of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijing100083China Key Laboratory of Intelligent Textile and Flexible Interconnection of Zhejiang Province Hangzhou310018China Department of Chemistry College of ScienceHebei Agriculture UniversityBaoding071001China School of Chemistry Beihang UniversityBeijing100191China Key Laboratory of Rare Earths Ganjiang Innovation AcademyChinese Academy of SciencesGanzhou341119China

Improving the electron/ion transport ability and alleviating expansion during charging/discharging processes are vital for lithium-ion batteries(liBs).In this work,a three-dimensional anode was fabricated using conductive hollow carbon-based nano tubes interpenetrated MXene architecture by directing the assembly of flexible electrospun hollow copper/carbon nanotubes and rigid Ti_(3)C_(2)T_(x) MXene *** introduction of copper into carbon matrix leads to an improvement of lithium storage owing to the increase of disorder ***,the unique structure of the fabricated electrode provides a cross-network for fast electron diffusion by preventing the stack of nanotubes and MXene ***,the optimized electrode exhibits a high initial capacity of 424.45mAh·g^(-1) and maintains at 378.05 mAh·g^(-1) with a current density of 5 A·g^(-1) after 1000 *** strategy of structural and chemical optimization provides new ideas for developing high-performance and durable electrochemical energy storage devices in the future.

关键词： Nanofiber Hollow structure Electrospinning Carbon materials lithium-ion battery

Machine learning identifies the risk of complications after laparoscopic radical gastrectomy for gastric cancer

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World Journal of Gastroenterology 2024年第1期30卷 79-90页

作者： qing-qi Hong Su Yan Yong-liang Zhao lin Fan li yang wen-Bin Zhang Hao liu He-xin lin jian Zhang zhi-jian Ye Xian Shen li-Sheng Cai guo-Wei Zhang Jia-Ming Zhu Gang Ji Jin-Ping Chen Wei wang Zheng-Rong li Jing-Tao Zhu guo-xin li jun You Department of Gastrointestinal Oncology Surgery The First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen 361001Fujian ProvinceChina Department of Gastrointestinal Surgery Qinghai University Affiliated HospitalXining 810000Qinghai ProvinceChina Department of General Surgery and Center of Minimal Invasive Gastrointestinal Surgery The First Hospital Affiliated to Army Medical UniversityChongqing 400038China Department of General Surgery The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061Shaanxi ProvinceChina Department of General Surgery The First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina Department of Gastrointestinal Surgery The First Affiliated Hospital of Xinjiang Medical UniversityUrmuqi 830054Xinjiang Uygur Autonomous RegionChina Department of General Surgery Nanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Department of Gastrointestinal Surgery Affiliated Hangzhou First People's HospitalZhejiang University School of MedicineHangzhou 310006Zhejiang ProvinceChina Department of Gastrointestinal Surgery Zhongshan Hospital of Xiamen UniversityXiamen 361004Fujian ProvinceChina Department of Surgery The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhou 325027Zhejiang ProvinceChina Department of General Surgery Unit 4 Zhangzhou Affiliated Hospital of Fujian Medical UniversityZhangzhou 363000Fujian ProvinceChina Department of Gastrointestinal Surgery The Second Affiliated Hospital of Xiamen Medical CollegeXiamen 361021Fujian ProvinceChina Department of Gastrointestinal Nutrition and Hernia Surgery The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina Department of Digestive Diseases Xijing HospitalAir Force Military Medical UniversityXi'an 710032Shaanxi ProvinceChina Department of Gastrointestinal Surgery Quanzhou First Hospital Affiliated to Fujian Medical UniversityQuanzhou 362002Fujian Provin

BACKGROUND Laparoscopic radical gastrectomy is widely used,and perioperative complications have become a highly concerned *** To develop a predictive model for complications in laparoscopic radical gastrectomy for gastric cancer to better predict the likelihood of complications in gastric cancer patients within 30 days after surgery,guide perioperative treatment strategies for gastric cancer patients,and prevent serious *** In total,998 patients who underwent laparoscopic radical gastrectomy for gastric cancer at 16 Chinese medical centers were included in the training group for the complication model,and 398 patients were included in the validation *** clinicopathological data and 30-d postoperative complications of gastric cancer patients were *** machine learning methods,lasso regression,random forest,and artificial neural networks,were used to construct postoperative complication prediction models for laparoscopic distal gastrectomy and laparoscopic total gastrectomy,and their prediction efficacy and accuracy were *** The constructed complication model,particularly the random forest model,could better predict serious complications in gastric cancer patients undergoing laparoscopic radical *** exhibited stable performance in external validation and is worthy of further promotion in more *** Using the risk factors identified in multicenter datasets,highly sensitive risk prediction models for complications following laparoscopic radical gastrectomy were *** hope to facilitate the diagnosis and treatment of preoperative and postoperative decision-making by using these models.

关键词： Gastric cancer Laparoscopic radical gastrectomy Postoperative complications Laparoscopic total gastrectomy