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Construction of One New 3D Zinc-organic Framework Based on D-(+)-camphoric Acid and Bis(4-pyridylmethyl)piperazine

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Chinese Journal of Structural Chemistry 2013年第12期32卷 1885-1889页

作者： DU Xi-Gang liu Shu-Hua liu ji-chun ZHANG Jun School of Chemical Engineering and Pharmaceutics Henan University of Science and Technology Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test CenterShandong Academy of Sciences

A new polymer [Zn2（cam）2（bpmp）]n 1 （H2cam = D-（＋）-camphoric acid, bpmp = bis（4-pyridylmethyl）piperazine） was synthesized under hydrothermal conditions. The compound crystallizes in the monoclinic system, space group P21/n with a = 12.305（9）, b = 12.945（9）, c = 13.619（10） A, β = 116.833（8）°, V= 1936（2）A3, Z = 4, Mr = 397.76, Dc= 1.365 Mg/m3,μ = 1.292 mm-1, F（000） = 832, the final R = 0.0578 and wR = 0.1678 for 2838 observed reflections with I 〉 2σ（I）. The compound presents a three-dimensional （3D） network extended by homochiral 2D layer and bpmp ligands. From the topology point of view, the structure could be described as a 3D 6-connected mab net with （44.610.8） topology. In addition, infrared spectroscopy, elemental analyses, thermogravimetric analysis and powder X-ray diffraction properties of 1 are also investigated.

关键词： metal-organic framework D-(+)-camphoric acid bis(4-pyridylmethyl)piperazine

A major quantitative trait locus controlling phosphorus utilization efficiency under different phytate-P conditions at vegetative stage in barley

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Journal of Integrative Agriculture 2018年第2期17卷 285-295页

作者： GAO Shang-qing CHEN Guang-deng HU De-yi ZHANG Xi-zhou LI Ting-xuan liu Shi-hang liu chun-ji College of Resources Sichuan Agricultural University Chengdu 611130 P.R. China Triticeae Research Institute Sichuan Agricultural University Chengdu 611130 P.R. China Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture Flagship St Lucia Queensland 4067 Australia

Organic phosphorus（P） is an important component of the soil P pool, and it has been proven to be a potential source of P for plants. The phosphorus utilization efficiency（PUE） and PUE related traits（tiller number（TN）, shoot dry weight（DW）, and root dry weight） under different phytate-P conditions（low phytate-P, 0.05 mmol L^-1 and normal phytate-P, 0.5 mmol L^-1） were investigated using a population consisting of 128 recombinant inbred lines（RILs） at the vegetative stage in barley. The population was derived from a cross between a P-inefficient genotype（Baudin） and a P-efficient genotype（CN4027, a Hordeum spontaneum accession）. A major locus（designated ***-3 H） conferring PUE was detected in shoots and roots from the RIL population. The quantitative trait locus（QTL） was mapped on chromosome 3 H and the allele from CN4027 confers high PUE. This locus explained up to 30.3 and 28.4% of the phenotypic variance in shoots under low and normal phytate-P conditions, respectively. It also explains 28.3 and 30.7% of the phenotypic variation in root under the low and normal phytate-P conditions, respectively. Results from this study also showed that TN was not correlated with PUE, and a QTL controlling TN was detected on chromosome 5 H. However, dry weight（DW） was significantly and positively correlated with PUE, and a QTL controlling DW was detected near the ***-3 H locus. Based on a covariance analysis, existing data indicated that, although DW may affect PUE, different genes at this locus are likely involved in controlling these two traits.

关键词： barley phosphorus utilization efficiency quantitative trait locus recombinant inbred line phytate-P

QTLs for Waterlogging Tolerance at Germination and Seedling Stages in Population of Recombinant Inbred Lines Derived from a Cross Between Synthetic and Cultivated Wheat Genotypes

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Journal of Integrative Agriculture 2014年第1期13卷 31-39页

作者： YU Ma MAO Shuang-lin CHEN Guo-yue liu Ya-xi LI Wei WEI Yu-ming liu chun-ji ZHENG You-liang Triticeae Research Institute Sichuan Agricultural University Agronomy College Sichuan Agricultural University CSIRO Plant Industry

Waterlogging is a widespread limiting factor for wheat production throughout the world. To identify quantitative trait loci （QTLs） associated with waterlogging tolerance at early stages of growth, survival rate （SR）, germination rate index （GRI）, leaf chlorophyll content index （CCI）, root length index （RLI）, plant height index （PHI）, root dry weight index （RDWI）, shoot dry weight index （SDWI）, and total dry weight index （DWI） were assessed using the International Triticeae Mapping Initiative （ITMI） population W7984/Opata85. Significant and positive correlations were detected for all traits in this population except RLI. A total of 32 QTLs were associated with waterlogging tolerance on all chromosomes except 3A, 3D, 4B, 5A, 5D, 6A, and 6D. Some of the QTLs explained large proportions of the phenotypic variance. One of these is the QTL for GRI on 7A, which explained 23.92% of the phenotypic variation. Of them, 22 alleles from the synthetic hexaploid wheat W7984 contributed positively. These results suggested that synthetic hexaploid wheat W7984 is an important genetic resource for waterlogging tolerance in wheat. These alleles conferring waterlogging tolerance at early stages of growth in wheat could be utilized in wheat breeding for improving waterlogging tolerance.

关键词： wheat waterlogging tolerance QTL germination and seedling stages

The Chromosomal Control of Leaf Characteristics of Early-Stage Plants in Wheat (Triticum aestivum L.)

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Agricultural Sciences in China 2011年第2期10卷 159-165页

作者： LI Cun-dong BAI Zhi-ying Ye G Miyagi M Chapman S Reader S M liu chun-ji CSIRO Plant Industry Queensland Bioscience PrecinctSt LuciaQLD 4067Australia Faculty of Agriculture Hebei Agricultural UniversityBaoding 071000P.R.China College of Life Science Hebei Agricultural UniversityBaoding 071000P.R.China School of Land and Food Sciences University of QueenslandSt LuciaQLD 4072Australia John Innes Centre Norwich Research ParkColney NorwichNR4 7UHUK

Rapid expansion of leaves of early-stage plants in wheat produced by chromosomal control of characteristics related to rapid expansion of the first six leaves of wheat were investigated using a set of single chromosome substitution lines under two different temperature regimes（TRs）.Results from this study indicated that several chromosomes could be responsible for each of the four characteristics studied（including leaf width,leaf length,leaf elongation rate and leaf appearance index） and that different chromosomes may be responsible for the same characteristics at different *** a small number of substitutions that showed the most significant effects on leaf length were found among those that showed the most significant effects on leaf width,providing further evidence that these two characteristics are likely controlled by different sets of *** 4B,4D,and 5A are among those that were repeatedly detected to having significant effects on different leaf characteristics at the lower-TR and genes residing on these three chromosomes could be *** possibility and its implication are discussed.

关键词： wheat leaf characteristics chromosomal control

QTL Mapping for Important Agronomic Traits in Synthetic Hexaploid Wheat Derived from Aegiliops tauschii ssp. tauschii

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Journal of Integrative Agriculture 2014年第9期13卷 1835-1844页

作者： YU Ma CHEN Guo-yue ZHANG Lian-quan liu Ya-xi liu Deng-cai WANG ji-rui PU Zhi-en ZHANG Li LAN Xiu-jin WEI Yu-ming liu chun-ji ZHENG You-liang Triticeae Research Institute Sichuan Agricultural University School of Life Science and Engineering Southwest University of Science and Technology Agronomy College Sichuan Agricultural University CSIRO Plant Industry St Lucia QLD 4067 Australia

Aegiliops tauschii is classified into two subspecies： Ae. tauschii ssp. tauschii and Ae. tauschii ssp. strangulata. Novel genetic variations exist in Ae. tauschii ssp. tauschii that can be utilized in wheat improvement. We synthesized a hexaploid wheat genotype（SHW-L1） by crossing an Ae. tauschii ssp. tauschii accession（AS60） with a tetraploid wheat genotype（AS2255）. A population consisting of 171 F8 recombinant inbred lines was developed from SHW-L1 and Chuanmai 32 to identify QTLs associated with agronomic traits. A new genetic map with high density was constructed and used to detect the QTLs for heading date, kernel width, spike length, spikelet number, and thousand kernel weight. A total of 30 putative QTLs were identified for five investigated traits. Thirteen QTLs were located on D genomes of SHW-L1, six of them showed positive effect on agronomic traits. Chromosome region flanked by wPt-6133–wPt-8134 on 2D carried five environment-independent QTLs. Each QTL accounted for more than 10% phenotypic variance. These QTLs were highly consistent across environments and should be used in wheat breeding.

关键词： genetic map QTL DArT agronomic traits synthetic wheat Aegilops tauschii ssp.tauschii

Activated Notch1 reduces myocardial ischemia reperfusion injury in vitro during ischemic postconditioning by crosstalk with the RISK signaling pathway

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Chinese Medical Journal 2013年第23期126卷 4545-4551页

作者： ZHOU Xue-liang WAN Li liu ji-chun Department of Cardiac Surgery First Affiliated Hospital Nanchang University Nanchang Jiangxi 330006 China

Background Ischemic postconditioning （IPost）,able to significantly attenuate myocardial ischemia reperfusion injury,is dependent on RISK *** have shown that Notch signaling repairs damaged myocardium,and this study aimed to investigate the effect of Notch signaling in myocardial *** We used H9c2 cells to establish the myocardial IPost and Hypoxia/Reoxygenation （H/R） model in vitro,which were randomly divided into control,H/R,IPost,Hepatocyte growth factor （HGF）＋IPost and DAPT＋IPost,N1ICD＋IPost,miRNA＋lPost,and Mock treatment *** myocardial cell viability was assessed by MTT,the cell apoptosis was detected using Annexin V/PI double staining and flow cytometry *** expression of N1ICD,Hes1,PTEN Phospho-Akt/Akt,Phospho-GSK-3β/GSK-3β were detected by Western ***,we assessed the changes in Ψm using the potential-sensitive dye JC-1 and measured using flow cytometry *** The Notch1 signaling is activated by HGF and ectopic expression of N1ICD during myocardial IPost,which increased myocardial cell viability,prevented cardiomyocyte apoptosis,and reduced loss of the mitochondrial membrane ***,myocardial ischemia reperfusion injury was increased in IPost when Notch1 signaling was inhibited using DAPT or with knockdown by *** blotting found that PTEN was down-regulated by Hes1 when Notch1 was activated,which consequently promoted Akt and GSK-3β *** Notch1 crosstalk with RISK signaling may be dependent on PTEN,which plays a cardioprotective role during *** mechanism could provide a promising therapeutic target for the treatment of ischemic heart disease.

关键词： Notch1 signaling ischemic postconditioning cardioprotection RISK signaling

Heat shock protein 70 gene transfection protects rat myocardium cell against anoxia-reoxygeneration injury

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Chinese Medical Journal 2007年第7期120卷 578-583页

作者： liu ji-chun HE Ming WAN Li CHENG Xiao-shu Department of Cardiovascular Surgery First Affiliated Hospital of Nanchang University Nanchang 330006 China Department of Pharmacology Medical College of Nanchang University Nanchang 330006 China Department of Cardiovascular Medicine Second Affiliated Hospital of Nanchang University Nanchang 330006 China

Background A number of studies suggest that the expression of heat shock protein 70 （HSP70） induced by heat stress are associated with protection against ischemia-reperfusion injury. But the protective effects may be contaminated by other factors in the same stress. This study was conducted to explore the protective role of HSP70 expression in acute myocardial anoxia/reoxygeneration （A/R） injury with a liposome-mediated gene transfer technique for the introduction of pCDNA HSP70 into the neonatal rat myocardial cells. In addition, heat shock stress cytoprotection was also investigated for comparison. Methods The cultured primary neonatal rat myocardiocytes with an acute myocardial A/R injury model and the HS-treated rat myocardiocyte model were used. Three-day cultured myocardiocytes were randomly divided into four groups （n=8）： control group, A/R group, HS＋A/R group and pCDNA HSP70 ＋A/R group. A liposome-coated HSP70 pCDNA plasmid was transfected into the primary neonatal rat myocardiocytes; HSP70 mRNA and its protein were confirmed by reverse transcriptase polymerase chain reaction （RT-PCR） and Western blotting. The cell viability was assayed by monotetrazolium （MTT） and the lactate dehydrogenase （LDH） and creatine phosphokinase （CPK） activity of cells during incubation and the changes in cells ultrastructure were examined. NF-κB activity in the primary neonatal rat myocardiocytes was measured with flow cytometry. Results Compared with viability in the A/R group （（35.4±6.9）%） the cell viability in the HS＋A/R group （（72.8±11.6）%） and the pCDNA HSP70 ＋ A/R group （（76.3±12.2）%） was improved significantly （P〈0.05）. The activity of LDH and CPK was significantly elevated in the A/R group. However, in the HS＋A/R group and pCDNA HSP70 ＋A/R group, significant decreases in activity were observed. The cell ultrastructure of the A/R group cells was abnormal, whereas nearly normal ultrastructure was observed in HS＋A/R group and pCDNA HSP70＋A/R group. HSP70 mRNA and protein

关键词： gene transfection HSP70 gene NF-κB cardiac myocyte anoxia-reoxygeneration injury

Effect of solute Ce,Mn,and Si on mechanical properties of silicon steel:insights from DFT calculations

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Journal of Iron and Steel Research International 2024年第3期31卷 700-709页

作者： Xiang-jun liu ji-chun Yang Hui-ping Ren Xiao-bin jia Ming-yi Zhang Chang-qiao Yang School of Materials and Metallurgy Inner Mongolia University of Science and TechnologyBaotou 014010Inner MongoliaChina Key Laboratory of Integrated Exploitation of Bayan Obo Multi-Metal Resource Inner Mongolia University of Science and TechnologyBaotou 014010Inner MongoliaChina Inner Mongolia North Heavy Industries Group Co. Ltd.Baotou 014010Inner MongoliaChina Inner Mongolia Metal Material Research Institute Baotou 014010Inner MongoliaChina

The influence of solute Ce,Mn,and Si on the mechanical properties of silicon steel was investigated by first-principles ***,Mn,and Si can all be solubilized in Fe *** significantly reduces the incompressibility and rigidity of the system but also significantly improves the toughness and *** effect of Mn on mechanical properties of the system is not *** has a significant effect on the improvement in incompressibility and rigidity but a limited effect on the improvement in toughness and *** metallic bond strength of Fe-Ce,Fe-Mn,and Fe-Si doped systems is weaker than that of the pure Fe system,which can be used to explain the reduction in the incompressibility and rigidity of these doped *** relatively high electron cloud density in the doped system may be responsible for the increase in toughness.

关键词： Silicon steel Rare earth First-principles calculation Elastic modulus Electronic structure

Pb induced dislocation defects of PtCo systems: Strain-triggered oxygen reduction reaction for PEMFC

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Nano Research 2024年第4期17卷 2462-2472页

作者： chun jin Qiheng Wang jingjun liu Beijing Key Laboratory of Electrochemical Process and Technology for Materials Beijing 100029China Beijing University of Chemical Technology Beijing 100029China

Design and development of advanced electrocatalysts with high performance and low Pt consumption are crucial for reducing the kinetic energy barrier of the cathode oxygen reduction reaction(ORR)and improving the efficiency of proton exchange membrane fuel cells(PEMFC).In this study,we demonstrate a Pb-modulated PtCo system for efficient ORR,in which the inclusion of Pb in ternary alloys induces dislocation defects due to the significant difference in atomic ***-PtCoPb was confirmed to exhibit significantly higher ORR activity and stability in acidic *** practical PEMFC applications,it outperforms the corresponding commercial Pt/C with a mass activity of 0.58 A·mgPt^(-1),making it a promising alternative to state-of-the-art Pt-based *** combination of experimental results and density functional theory(DFT)calculations offers valuable atomic-level insights into the dislocation *** with a larger atomic radius is located in the lattice stretching region below the dislocation slip plane,forming a structure similar to a Cottrell atmosphere,which reduces the dislocation energy and puts the system in a lower energy *** Cottrell atmosphere pins the dislocation structure and stabilizes the ternary *** adjusting the amount of added Pb,a moderate level of dislocation density induces a tuned strain effect,thereby enhancing the electrocatalytic mechanism by optimizing the electronic structure of the alloy surface and the adsorption and desorption of oxygen *** work provides valuable insights into the design and development of lattice dislocation defect structures to trigger strain effects for improving ORR performance.

关键词： dislocation defect strain effect Pb-modulated oxygen reduction reaction