检索结果-南通市图书馆

A High-Affinity Methyl-CpG-Binding Protein for Endonuclease-Free and Label-Free DNA Methyltransferase Activity Detection

在线全文

学校读者我要写书评

暂无评论

Journal of Analysis and Testing 2024年第3期8卷 327-334页

作者： Yang Bai Shulin Tan Yingsong Sheng Yueqing Gu haiping Wu Baicun Li Yunlong liu department of Biomedical engineering School of Engineering China Pharmaceutical University department of Clinical Pharmacy Jinling Hospital State Key Laboratory of Analytical Chemistry for Life Science & Jiangsu Key Laboratory of Molecular Medicine Medical School of Nanjing University School of Pharmaceutical sciences Southern Medical University National Center for Respiratory Medicine Center of Respiratory Medicine China-Japan Friendship Hospital

DNA methyltransferase(MTase) activity detection has received increasing attention as a promising biomarker and therapeutic target. However, most of these detection methods rely on endonuclease digestion and signal groups ***, we present a novel platform for sensing DNA MTase activity that overcomes these limitations. Our approach is both endonuclease-free and label-free, utilizing a combination of a high-affinity streptavidin-methyl-CpG-binding domain(SA-MBD) protein and surface plasmon resonance(SPR) technology. The SA-MBD protein specifically recognizes a hairpin probe containing methylated CpG sites, which is treated with *** MTase. This recognition event generates a corresponding SPR response signal. The limit of detection is as low as 0.016 U/mL, owing to the high-a ffinity of the SA-MBD ***, we have demonstrated the feasibility of our method for *** MTase activity analysis in serum and inhibitor screening, which implies the potential prospects for biomedical research.

关键词： Biosensing DNA methyltransferase Inhibitor screening Methyl-CpG-binding protein surface plasmon resonance

Efficient water disinfection with ball milled Mg-biochar: The key role of trace Cu

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第1期35卷 412-417页

作者： Yanchao Jin Suixiaochen Chen Peiwen Huang Xiongjian Chen Chun-Yan Lin Li-Ping Li Xiao Chen Rui Ding Jianxi liu Riyao Chen College of Environmental and Resource sciences Fujian Normal UniversityFuzhou 350117China School of Materials and Chemical engineering Minjiang UniversityFuzhou 350108China Research and Development Center for Watershed Environmental Eco-engineering Beijing Normal UniversityZhuhai 519087China Fujian Key Laboratory of Pollution Control&Resource Reuse Fuzhou 350007China

In this study, magnesium and coconut shell carbon (CSC) were prepared by a ball milled process and used for water disinfection with adsorbing tiny amounts of copper(II). Dissolved oxygen (DO) was reduced to hydrogen peroxide (H_(2)O_(2)) via a two-electron pathway by Mg corrosion. Cu(II) in the wastewater will be enriched on the CSC surface and efficiently catalyzes H2O2 for inactivating E. coli. The results show that E. coli with an initial concentration of approximately 106 CFU/mL was under the detection limit (<4 CFU/mL) within 15 min. All of the Cu(II) could be adsorbed by the composite and catalyzed H2O2 to different active species. The quenching experiments, electron spin resonance (ESR) capture measurements and the UV-vis spectroscopy detection confirmed the present of the hydroxyl radicals (·OH), superoxide radicals (·O_(2)^(-)) and Cu(III). Different with tradition Fenton like process, Cu(III), rather than radicals, played the major role during the Mg-CSC/Cu(II) process. In addition to the cellular membrane damage, most of the bacterial genomic DNA was also be degraded and the bacterial reactivation was avoided. The Mg-CSC/Cu(II) process also showed a satisfied disinfection performance in real wastewater treatment. Overall, this study provides a new strategy for water disinfection.

关键词： Disinfection Magnesium Cu Hydrogen peroxide Biochar

Ion enrichment on the hydrophobic carbon-based surface in aqueous salt solutions due to cation-πinteractions

在线全文

学校读者我要写书评

暂无评论

Ion enrichment on the hydrophobic carbon-based surface in aq...

Recent progress in superhydrophobic coating on Mg alloys:A general review

中国力学大会——2013

作者： Shi Guosheng liu Jian Wang Chunlei o Song B Tu Yusong Hu Jun Fang haiping department of Water science and technology and department of Physical Biology Shanghai Institute of Applied PhysicsChinese Academy of Sciences Graduate School of the Chinese Academy of sciences Institute of Systems Biology Shanghai University

An understanding of the ion distribution at a solid-liquid interface,a gas-liquid interface,and in the bulk is of great importance in various fields,including the atmospheric,materials, chemical,and biological ***,interac- tions between ions and carbon-based surfaces are usually considered within the general van der Waals interactions,and their effects have been ***,we show that there is a clear enrichment of Na on a hydrophobic carbon-based surface in a NaCl solution using molecular dynamics simulations

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Magnesium and Alloys 2021年第5期9卷 1479-1496页

作者： Feng Peng Dongdong Zhang Xuanyong liu Yu Zhang department of Orthopedics Guangdong Provincial People's HospitalGuangdong Academy of Medical SciencesGuangzhou 510080GuangdongChina State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China Center of Materials science and Optoelectronics engineering University of Chinese Academy of SciencesBeijing 100049China Cixi Center of Biomaterials surface engineering Shanghai Institute of CeramicsChinese Academy of SciencesNingbo 315300China

Magnesium(Mg)is a vital engineering material owing to its light weight and excellent mechanical ***,poor corrosion resistance limits its widely applications as well as its economic ***,surface modification is essential for Mg and its *** the various coatings,superhydrophobic coating,which is inspired by nature,has received increasing attentions in the past *** a water contact angle larger than 150°,superhydrophobic coating can provide sufficient protection for Mg-based *** model of superhydrophobic states and the protection mechanism of superhydrophobic coating are discussed in this ***,the methods for fabricating superhydrophobic coatings on Mg alloys are ***,some functional superhydrophobic coatings on Mg alloys are ***,the challenges and future directions are *** hope that this paper will provide a serviceable review for future research on superhydrophobic coatings on Mg alloys.

关键词： Magnesium alloys Superhydrophobic coatings corrosion resistance

Liquid metal corrosion sculpture to fabricate quickly complex patterns on aluminum

在线全文

学校读者我要写书评

暂无评论

science china(Technological sciences) 2017年第1期60卷 65-70页

作者： LU JinRong YI LiTing WANG Lei TAN SiCong GUI Han liu Jing department of Biomedical engineering School of Medicine Tsinghua University Beijing 100084 China

A liquid metal enabled corrosion sculpture technique for quickly fabricating complex pattems on aluminum substrate is proposed and experimentally demonstrated. According to the conceptual investigation, it is clarified that the width and the depth of a printed track are dominated by sculpture time and working temperature. The printed size can reach small values of 38 μm through controlling the sculpture time for 60 s. As the sculpture time was increased from 5 to 25 rain at 20℃, the depth of the fabricated pattern was improved from 13.3 to 25.6 μm. The sculptured depth of the pattern would increase from 13.3 to 106.9 μm when the sculpture time was fixed at 5 min and the temperature was raised from 20 to 60℃. To investigate the sculpture behavior in detail, the phases and microstructure of sculpture surface were quantitatively measured via a group of microscope imaging system with fundamental mechanisms interpreted. The present liquid metal sculpture method on aluminum substrate adds a new valuable soft tool for current metal engraving technology family.

关键词： sculpture printing corrosion liquid metal aluminum substrate soft fabrication

Early Stage of Oxidation on Titanium surface by Reactive Molecular Dynamics Simulation

在线全文

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2018年第4期55卷 177-188页

作者： Liang Yang Caizhuang Wang Shiwei Lin Yang Cao Xiaoheng liu School of chemical engineering Nanjing University of science&TechnologyNanjing 210094China Materials and Chemical engineering Hainan UniversityHaikou570228China Ames laboratory and department of Physics Iowa State UniversityAmesIA 50011USA

Understanding of metal oxidation is very critical to corrosion control,catalysis synthesis,and advanced materials *** oxidation is a very complex phenomenon,with many different processes which are coupled and involved from the onset of *** this work,the initial stage of oxidation on titanium surface was investigated in atomic scale by molecular dynamics(MD)simulations using a reactive force field(ReaxFF).We show that oxygen transport is the dominant process during the initial *** simulation also demonstrate that a compressive stress was generated in the oxide layer which blocked the oxygen transport perpendicular to the Titanium(0001)surface and further prevented oxidation in the deeper *** mechanism of initial oxidation observed in this work can be also applicable to other self-limiting oxidation.

关键词： Reactive force field metal oxidation self-limiting oxidation Titanium(0001)surface molecular dynamics simulation compressive stress

Temperature and NaCl deposition dependent corrosion of SAC305 solder alloy in simulated marine atmosphere

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials science & technology 2021年第16期75卷 252-264页

作者： Chuang Qiao Mingna Wang Long Hao Xiahe liu Xiaolin Jiang Xizhong An Duanyang Li School of Metallurgy Northeastern UniversityShenyang110819China Environmental corrosion Centre of Materials Institute of Metal ResearchChinese Academy of SciencesShenyang110016China department of Physics Hebei Normal University of Science technologyQinhuangdao066004China School of Material science and engineering University of Science and Technology of China.Hefei230026China College of Mechanical engineering Institute of Innovative Science and TechnologyShenyang UniversityShenyang110044.China

The stable operation of electronic devices in marine atmospheric environment is affected by the corrosion deterioration of solder joints,and the effects by atmosphere temperature and chloride deposition are *** this work,NaCl deposition and temperature dependent corrosion of Pb-free SAC305 solder in simulated marine atmosphere has been *** results indicate that higher NaCl deposition prolongs the surface wetting time and leads to the final thicker saturated electrolyte film for further *** temperature accelerates the evaporation and contributes to the final thinner saturated NaCl electrolyte ***,the corrosion control process varies under the initially covered thicker NaCl electrolyte layer and under the final saturated much thinner NaCl electrolyte film as the evaporation ***,the ready oxygen availability through the final thinner saturated NaCl electrolyte film facilitates the formation of corrosion product layer mainly of electrochemically stable SnO2,but higher temperature leads to the final corrosion product layer with smaller crystal size and large *** findings clearly demonstrate the effects of NaCl deposition and temperature on corrosion evolution of SAC305 solder joints and are critical to the daily maintenance of electronic devices for longer service life in marine atmosphere.

关键词： Atmospheric corrosion SAC305 solder Marine atmosphere NaCl Temperature

Preliminary study of adsorption behavior of bovine serum albumin(BSA) protein and its effect on antibacterial and corrosion property of Ti-3Cu alloy

在线全文

学校读者我要写书评

暂无评论

Journal of Materials science & technology 2021年第21期80卷 117-127页

作者： Muhammad Ali Siddiqui Ihsan Ullah Hui liu Shuyuan Zhang Ling Ren Ke Yang Institute of Metal Research Chinese Academy of Sciences72 Wenhua RoadShenyang110016China School of Materials science and engineering University of Science and Technology of China96 Jinzhai RoadHefei230026China department of Metallurgical engineering NED University of Engineering and TechnologyUniversity RoadKarachi75270Pakistan

The adsorption behavior, antibacterial, and corrosion properties of a Ti-3 Cu alloy were studied in a phosphate-buffered saline solution containing 0, 1, 3, and 6 gL^(-1) bovine serum albumin protein at 37℃ and pH = 7.4(±0.2). The protein adsorption behavior was examined via cyclic voltammetry, secondary ions mass spectroscopy(SIMS), and angle-resolved X-ray photoelectron spectroscopy(ARXPS). The corrosion property was analyzed by the open circuit potential(OCP), potentiodynamic polarization(PD),and electrochemical impedance spectroscopy(EIS) examinations. The antibacterial test was conducted according to the GB/T 21510 china Standard. It was observed that the surface charge density(QA DS) was directly proportional to the amount of the adsorbed BSA protein, signifying that the protein adsorption was accompanied by the charge transfer, pointing to chemisorptions phenomena. BSA amino groups and other organic species were observed in the surface analysis examinations. It was shown that the formation of barrier complexes between the TiO_(2) oxide-layer and PBS solution resulted in decreasing the release of Cu-ions, which consequently reduced the antibacterial activity. On the other hand, these barrier complexes improved the corrosion resistance by increasing the charge transfer resistance and double-layer capacitance of the Ti-3 Cu alloy.

关键词： Bovine serum albumin protein Ti-3Cu alloy Antibacterial corrosion Adsorption behavior

Effect of Silicon and Aluminum Addition on corrosion Behavior of ODS Iron-Based Alloys in Liquid Lead–Bismuth Eutectic

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2023年第5期36卷 732-744页

作者： Jing Li Xiaochen Zhang Haibin Ma Liangyin Xiong Shi liu Qisen Ren Zhengzheng Pang Key Laboratory of Nuclear Materials and Safety Assessment Institute of Metal ResearchChinese Academy of SciencesShenyang110016China Shi-Changxu Innovation Center for Advanced Materials Institute of Metal ResearchChinese Academy of SciencesShenyang110016China School of Materials science and engineering University of Science and Technology of ChinaShenyang110016China Nuclear Fuel and Materials department China Nuclear Power Technology Research InstituteShenzhen518026China

The long-term corrosion behaviors of four variants of oxide dispersion strengthened(ODS)iron-based alloys in the stagnant oxygen-saturated lead–bismuth eutectic(LBE)at 550℃ were studied *** effects of silicon and aluminum content on the thickness,morphology and composition of the oxide scale were explored with the aid of X-ray diffraction(XRD),scanning electron microscopy(SEM),electron probe micro-analyzer(EPMA)and X-ray photoelectron spectroscopy(XPS).The addition of 1.5 wt%silicon is not able to contribute to forming a protective external silicon oxide film on the surface of aluminum-free ODS iron-based alloy,while the addition of aluminum promotes the formation of a thin and continuous alumina oxide *** the meantime,an appropriate amount of silicon becomes the heterogeneous nucleation site for alumina during the initial stage of oxidation,giving rise to the rapid formation of a protective alumina ***,excessive silicon has a negative impact on the formation of continuous alumina scale,because it may compete with aluminum to absorb more *** result of oxidation kinetics in ODS iron-based alloy shows that the parabolic rate constant of the alumina oxide scale is 3–4 orders of magnitude lower than that of the scale mainly composed of iron and chromium oxide.

关键词： Pb-Bi corrosion Oxide dispersion strengthened(ODS)ferritic alloy Silicon and aluminum addition Oxide scale