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Changes in the microbial community structure and diversity during the electricity generation process of a microbial fuel cell with algal-film cathode

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Clean Energy 2024年第5期8卷 177-185页

作者： haiping Wang Liguo Zheng Changyin Tan Ling Li Feng liu Hui Feng Changsha Environmental Protection Vocational College Changsha 410004HunanChina School of Geographic Sciences Hunan Normal UniversityChangsha 410081HunanChina Key Laboratory of Agro-Ecological Processes in Subtropical Regions Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina

A two-chamber microbial fuel cell(MFC)with algal-film cathode was *** showed good electric-generating performance with three electric-generating stages:start-up,development,and *** average output voltage reached~0.412 V during the stable period.A maximum power density during continuous operation was 19.76 mW/m^(2).Bacterial samples were collected from the anode in the three stages(A1,A2,and A3),and their community structure and diversity were analyzed using Illumina MiSeq high-throughput sequencing technology.A total of 4238 operational taxonomic units were identified based on the number of *** the phylum level,Proteobacteria and Bacteroidetes played a dominant role in the three stages and increased significantly during electricity *** with A1,the relative abundances of Proteobacteria in A2 and A3 increased by 23.30%and 32.06%,respectively,whereas those of Bacteroidetes in A2 and A3 increased by 5.56%and 14.50%,*** the genus level,there were differences in the composition of bacterial communities among the three *** and Chlorobium became the dominant genera in A2,replacing Nitrospira and norank_f__Saprospiraceae in A1,and Sphingobacterium and Ochrobactrum became the dominant genera in *** to the sample cluster and principal component analyses,A1 was clustered into one class,and A2 and A3 were clustered into a second *** work revealed bacterial community succession at the anode of an algal-film cathode MFC during the electricity generation process,which provides a theoretical basis for the subsequent promotion of electricity generation by algal-film cathode MFCs.

关键词： microbial fuel cell electricity generation high-throughput sequencing bacterial community

BLE beacons for sample position estimation in a life science automation laboratory

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Transportation Safety and Environment 2024年第3期6卷 111-123页

作者： haiping Wu Steffen Junginger Thomas Roddelkopf Hui liu Kerstin Thurow Institute of Artificial Intelligence&Robotics(IAIR) Key Laboratory of Traffic Safety on Track of Ministry of EducationSchool of Traffic and Transportation EngineeringCentral South UniversityChangsha 410075HunanChina Institute of Automation(IAT) University of Rostock18119 RostockGermany Center of Life Science Automation(celisca) University of Rostock18119 RostockGermany

Estimation of the sample position is essential for working process monitoring and management in the life science automation lab*** low-energy(BLE)beacons have the advantages of low price,small size and low energy consumption,which make them a promising solution for sample position estimation in the automated *** fingerprinting models have been proposed to achieve indoor localization with the received signal strength(RSS)***,most of the research depends on intensive beacon *** estimation,which depends entirely on one beacon,is more suitable for sample position estimation in large automated *** complexity of the life science automation laboratory environment brings challenges to the traditional path loss model(PLM),which is a widely used radio wave propagation model-based proximity estimation *** this paper,BLE sensing devices for sample position estimation are *** BLE beacon-based proximity estimation is discussed in the framework of machine learning,in which the support vector regression(SVR)is utilized to model the nonlinear relationship between the RSS data and distance,and the Kalman filter is utilized to decrease the RSS data *** experimental results over different environments indicate that the SVR outperforms the PLM significantly,and provides 1 m absolute errors for more than 95%of the testing *** Kalman filter brings benefits to stable distance *** from proximity-based sample position estimation,the proposed framework turned out to be effective in position estimation between parallel workbenches and position estimation on an automated workstation.

关键词： BLE beacon position estimation proximity estimation received signal strength(RSS) support vector regression(SVR) life science automation laboratory

石墨烯水性环氧防腐涂料技术在电化学工程实验教学中的应用

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广东化工 2024年第18期51卷 240-243页

作者：刘海萍毕四富熊云奇姜杰滕祥国刘德丽曹立新哈尔滨工业大学(威海) 山东威海264209

将石墨烯水性环氧防腐涂料技术设计应用于本科生的电化学工程实验,使前沿的科研成果和电化学实验教学有机结合,让学生充分了解涂层技术在金属防腐蚀领域应用进展,可进一步巩固学生的专业知识和实验技能,提高学生从事科研的实际操作能力... 详细信息

将石墨烯水性环氧防腐涂料技术设计应用于本科生的电化学工程实验,使前沿的科研成果和电化学实验教学有机结合,让学生充分了解涂层技术在金属防腐蚀领域应用进展,可进一步巩固学生的专业知识和实验技能,提高学生从事科研的实际操作能力。有利于拔尖创新复合型人才的培养,充分体现了科研成果对实验教学和人才培养的支撑作用。

关键词：电化学工程实验实验教学石墨烯水性环氧涂料

Cation-πinteractions regulate electrocatalytic water oxidation over iridium single atoms supported on conjugated polymers

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Science China Chemistry 2024年第6期67卷 2063-2069页

作者： Jing Bai Yunxia liu Ziwei Ma Shouhan Zhang Guojie Chao haiping Lin Elke Debroye Longsheng Zhang Tianxi liu Key Laboratory of Synthetic and Biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan UniversityWuxi214122China School of Chemistry and Chemical Engineering Northwestern Polytechnical UniversityXi’an710072China School of Physics and Information Technology Shanxi Normal UniversityXi’an710062China Department of Chemistry KU LeuvenCelestijnenlaan 200FLeuven3001Belgium

Designing cost-effective and high-performing metal catalysts is significant for many renewable energy conversion *** metal loading without sacrificing activity and durability is highly desired for the catalyst design,especially for those reactions where the noble metals deliver the best catalyzing ***-atom catalysts(SACs)with maximal metalatom utilization,homogeneous and tailorable active sites have emerged as promising catalyst candidates,where the local coordination structures of the metal atoms in SACs largely determine the reaction *** design strategies of constructing strong metal-support interactions can stabilize the individual metal atoms in SACs,but present obstacles to provide a flexible manipulation platform for elaborately tailoring the coordination structures to achieve performance optimization towards a specifically targeted ***,for the proof-of-concept study,we report a novel design of SAC with iridium(Ir)single atoms supported on conjugated polymer,in which the adsorption energies of reaction intermediates on Ir atoms and the reaction kinetics towards acidic water oxidation can be readily optimized through modulating the formed cation-πinteractions that can be tailored by adjusting the molecular structures of conjugated *** strategy establishes a general route to develop targeted SACs for various catalytic reactions.

关键词： conjugated polymers single-atom catalysts cation-πinteractions oxygen evolution reaction

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色林错裸鲤染色体组构成及倍性分析

水产科学 2024年第2期43卷 297-303页

作者：李信马可馨郭文轩李逸帆杨悦瑶周子昱刘海平肖世俊周贺大连海洋大学水产与生命学院辽宁大连116023 西藏自治区农牧科学院水产科学研究所西藏拉萨850000 嘉兴市经济菌物新种质资源创制重点实验室浙江嘉兴314000

西藏地区严酷的高原环境孕育出了独特而丰富的生物资源,其中裂腹鱼亚科鱼类其各属种间不同类群的染色体表现出多样性特征。以体质量246.0~374.2 g的雄性色林错裸鲤肾脏组织细胞为材料,采用常规冷滴片法制备染色体标本,通过吉姆萨染色,... 详细信息

西藏地区严酷的高原环境孕育出了独特而丰富的生物资源,其中裂腹鱼亚科鱼类其各属种间不同类群的染色体表现出多样性特征。以体质量246.0~374.2 g的雄性色林错裸鲤肾脏组织细胞为材料,采用常规冷滴片法制备染色体标本,通过吉姆萨染色,确定其染色体数目、核型;通过银染显带(Ag-NORs)技术及荧光染料色霉素A(CMA3)和4,6-二脒基-2-苯基吲哚(DAPI)双重荧光染色技术,推测其带型及倍性。结果表明:色林错裸鲤染色体数目为4n=88,核型公式为40m+20sm+28st/t,NF=148。通过Ag-NORs染色观察,色林错裸鲤间期核最高银染点数为4个,有丝分裂中期染色体分裂相银染点数最高为4个,且信号点2强2弱,位于第2组亚中部着丝粒染色体(sm2)短臂上,为端部Ag-NORs。经CMA3/DAPI双重荧光染色,有丝分裂中期分裂相显示有4个CMA3阳性部位,且信号点2强2弱,位于第2组亚中部着丝粒染色体(sm2)短臂的端部区域。因此,推测色林错裸鲤是含有4套染色体组的遗传四倍体。本研究结果补充了色林错裸鲤细胞遗传学方面研究的空白,可为物种间亲缘关系的研究提供重要依据,并为后续开展裂腹鱼亚科鱼类分类和进化地位相关研究提供参考。

关键词：色林错裸鲤核型分析 Ag-NORs CMA3/DAPI 倍性分析

Effects of Mogroside V on Glucose and Lipid Metabolism in High Fat Diet Mice

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Medicinal Plant 2022年第3期13卷 36-38,42页

作者： Riming WEI haiping liu Pharmacology Laboratory of Prevention and Treatment of High Incidence of Disease Guilin Medical University Guilin 541199 China

[Objectives]To explore the effects and mechanism of mogroside V(MV)on glucose and lipid metabolism in high-fat diet(HFD)mice.[Methods]The experiment fed mice with high-fat diet for 8 weeks,and 40 mice with successful modeling were randomly divided into normal group,model group,and MV dose group(100,200 mg/kg),with 10 mice in each *** the ninth week,the MV dose group was given intragastric administration,and the normal group and the model group were given an equal volume of distilled water by intragastric administration for 6 weeks,then killed and blood samples and livers were *** triglycerides(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),free fatty acids(FFA),Advanced glycation end products(AGE-P)-peptides(AGE-P)and glycosylated hemoglobin(HbA1c)content,and TG and hepatic glycogen content in liver were detected by biochemical *** blood glucose(FBG)was measured by glucose oxidase *** fasting serum insulin(FINS)content was detected by enzyme-linked immunosorbent assay(ELISA),and the insulin resistance index(HOMA-IR)was *** red O staining was used to observe the fat deposition in liver tissue.[Results]MV(100,200 mg/kg)dose groups could significantly down-regulate the contents of TC,TG,LDL-C,FBG,FINS,AGE-P and HbA1c and HOMA-IR,and up-regulate HDL-C and hepatic glycogen content and reduce the fat deposits.[Conclusions]The mechanism of MV regulating glucose and lipid metabolism in mice may be related to the regulation of insulin resistance.

关键词： Mogroside V(MV) High fat diet(HFD) Glucose and lipid metabolism Insulin resistance

MicroRNAs in tomato plants

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Science China(Life Sciences) 2011年第7期54卷 599-605页

作者： ZUO JinHua WANG YunXiang liu haiping MA YuanZheng JU Zheng ZHAI BaiQiang FU DaQi ZHU Yi LUO YunBo ZHU BenZhong College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China

MicroRNAs (miRNAs) are a specialized class of small silencing RNAs that regulate gene expression in *** plants,miRNAs negatively regulate target mRNAs containing a highly complementary sequence by either mRNA cleavage or translational *** a model plant to study fleshy fruit ripening,miRNA studies in tomato have made great progress *** were predicted to be involved in nearly all biological processes in tomato,particularly development,differentiation,and biotic and abiotic stress ***,several miRNAs were verified to be involved in tomato fruit ripening and *** studies suggest that miRNAs are related to host-virus interactions,which raises the possibility that miRNAs can be used as diagnostic markers for response to virus infection in tomato *** this review,we summarize our current knowledge systematically and advance future directions for miRNA research in tomato.

关键词： microRNAs tomato research methods target gene prediction functional analysis

A High-Affinity Methyl-CpG-Binding Protein for Endonuclease-Free and Label-Free DNA Methyltransferase Activity Detection

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Journal of Analysis and Testing 2024年第3期8卷 327-334页

作者： Yang Bai Shulin Tan Yingsong Sheng Yueqing Gu haiping Wu Baicun Li Yunlong liu Department of Biomedical Engineering School of Engineering China Pharmaceutical University Department of Clinical Pharmacy Jinling Hospital State Key Laboratory of Analytical Chemistry for Life Science & Jiangsu Key Laboratory of Molecular Medicine Medical School of Nanjing University School of Pharmaceutical Sciences Southern Medical University National Center for Respiratory Medicine Center of Respiratory Medicine China-Japan Friendship Hospital

DNA methyltransferase(MTase) activity detection has received increasing attention as a promising biomarker and therapeutic target. However, most of these detection methods rely on endonuclease digestion and signal groups ***, we present a novel platform for sensing DNA MTase activity that overcomes these limitations. Our approach is both endonuclease-free and label-free, utilizing a combination of a high-affinity streptavidin-methyl-CpG-binding domain(SA-MBD) protein and surface plasmon resonance(SPR) technology. The SA-MBD protein specifically recognizes a hairpin probe containing methylated CpG sites, which is treated with *** MTase. This recognition event generates a corresponding SPR response signal. The limit of detection is as low as 0.016 U/mL, owing to the high-a ffinity of the SA-MBD ***, we have demonstrated the feasibility of our method for *** MTase activity analysis in serum and inhibitor screening, which implies the potential prospects for biomedical research.

关键词： Biosensing DNA methyltransferase Inhibitor screening Methyl-CpG-binding protein Surface plasmon resonance

辨证施治联合针刺治疗中风恢复期失语的临床研究

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中外医药研究 2024年第3期3卷 102-104页

作者：刘海平王艳临沂市中心医院山东临沂274000 菏泽市中医医院山东菏泽274000

目的:分析中医辨证疗法联合针刺治疗中风恢复期失语症的临床效果。方法:选择2019年5月-2020年5月菏泽市中医医院收治的中风恢复期失语症患者80例作为研究对象,随机数字表法将患者分为对照组和观察组,各40例。对照组接受常规中医辨证治疗... 详细信息

目的:分析中医辨证疗法联合针刺治疗中风恢复期失语症的临床效果。方法:选择2019年5月-2020年5月菏泽市中医医院收治的中风恢复期失语症患者80例作为研究对象,随机数字表法将患者分为对照组和观察组,各40例。对照组接受常规中医辨证治疗,观察组接受中医辨证联合针刺治疗。比较两组患者治疗失语症的效果。结果:观察组患者治疗后语言能力评分、总体治疗有效率高于对照组,差异有统计学意义(P<0.05)。结论:对脑中风后康复期的失语症进行中药辨证施治,可以明显提高患者的言语能力,取得了很好的疗效。

关键词：中风失语症中医辨证治疗针刺