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Influence of high-molecular-weight glutenin subunit deletions at the Glu-A1 and Glu-D1 loci on protein body development, protein components and dough properties of wheat(Triticum aestivum L.)

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Journal of Integrative Agriculture 2022年第7期21卷 1867-1876页

作者： liu da-tong ZHANG Xiao JIANG Wei LI Man WU Xu-jiang GAO De-rong BIE tong-de LU Cheng-bin Key Laboratory of Wheat Biology and Genetic Improvement for Low&Middle Yangtze Valley Ministry of Agriculture and Rural Affairs/Lixiahe Institute of Agricultural Sciences of JiangsuYangzhou 225007P.R.China

High-molecular-weight glutenin subunits(HMW-GSs) play a critical role in determining the viscoelastic properties of wheat. As the organelle where proteins are stored, the development of protein bodies(PBs) reflects the status of protein synthesis and also affects grain quality to a great extent. In this study, with special materials of four near-isogenic lines in a Yangmai 18 background we created, the effects of Glu-A1 and Glu-D1 loci deletions on the development and morphological properties of the protein body, protein components and dough properties were investigated. The results showed that the deletion of the HMW-GS subunit delayed the development process of the PBs, and slowed the increases of volume and area of PBs from 10 days after anthesis(daA) onwards. In contrast, the areas of PBs at 25 daA, the middle or late stage of endosperm development, showed no distinguishable differences among the four lines. Compared to the wild type and single null type in Glu-A1, the ratios of HMW-GSs to low-molecular-weight glutenin subunits(LMW-GSs), glutenin macropolymer(GMP) content, mixograph parameters as well as extension parameters decreased in the single null type in Glu-D1 and double null type in Glu-A1 and Glu-D1, while the ratios of gliadins(Gli)/glutenins(Glu) in those types increased. The absence of Glu-D1 subunits decreased both dough strength and extensibility significantly compared to the Glu-A1 deletion type. These results provide a detailed description of the effect of HMW-GS deletion on PBs, protein traits and dough properties, and contribute to the utilization of Glu-D1 deletion germplasm in weak gluten wheat improvement for use in cookies, cakes and southern steamed bread in China and liquor processing.

关键词： wheat near-isogenic lines HMW-GS deletion protein body dough property

Effects of Ethephon on Aerenchyma Formation in Rice Roots

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Rice science 2009年第3期16卷 210-216页

作者： KONG Yu WANG Zhong CHEN Juan PAN Xue-tong liu da-tong ZHANG Er-jin Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Crop Physiology Ecology and Cultivation in the Middle and Lower Reaches of the Yangtze River Ministry of Agriculture Yangzhou University Yangzhou 225009 China College of Biosciences and Biotechnology Yangzhou University Yangzhou 225009 China College of Life Science and Engineering Huanggang Normal University of Hubei Province Huanggang 438000 China

The effects of ethephon on the constitutive aerenchyma formation in roots were studied with a rice variety Yangdao 6 as material. The number of air spaces formed by disintegrated cells in mediopellis increased significantly with the rising ethephon concentrations, whereas superoxide dismutase （SOD） activity showed downward trends. Compared with the control, the expression levels of xyloglucan endotransglycosylase （XED gene were markedly higher both at the apical 10 mm and distal parts of roots in 100 mg/L ethephon treated plants. The accumulation of XET was supposed to be associated with the aerenchyma development. Furthermore, earlier cortical cell death was observed under the ethephon treatments, and most of nuclei of cells at 4 mm from the root apex disintegrated with many Golgi apparatus, mitochondria and membranebound vesicles around the cell wall.

关键词： rice root ethephon constitutive aerenchyma xyloglucan endotransglycosylase gene superoxide dismutase

Effect of high-molecular-weight glutenin subunit deletion on soft wheat quality properties and sugar-snap cookie quality estimated through near-isogenic lines

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Journal of Integrative Agriculture 2018年第5期17卷 1066-1073页

作者： ZHANG Xiao ZHANG Bo-qiao WU Hong-ya LU Cheng-bin Lü Guo-feng liu da-tong LI Man JIANG Wei SONG Gui-hua GAO De-rong Key Laboratory of Wheat Biology and Genetic Improvement for Low and Middle Yangtze River Valley Wheat Region Ministry of Agriculture/Yangzhou Academy of Agricultural Sciences

High-molecular-weight glutenin subunits（HMW-GSs） play a critical role in determining the viscoelastic properties of wheat dough. The HMW-GSs are encoded by Glu-A1, Glu-B1, and Glu-D1 loci on the long arms of chromosomes 1A, 1B, and 1D, respectively. In the present study, four near-isogenic lines with different HMW-GS deletions and compositions at the Glu-A1 and Glu-D1 loci in Yangmai 18 background were used for quality analysis. Deletion in Glu-D1 showed much weaker gluten quality and dough strength than null Glu-A1 genotype and wild genotype（WT）, based on the measurements of sodium dodecyl sulfate（SDS）-sedimentation, lactic acid solvent retention capacity（SRC）, gluten index, development time, stability time, and alveograph P and L values. The deletion of Glu-D1 did not significantly affect grain hardness, grain protein content, water SRC, sodium carbonate SRC, and sucrose SRC. Double null genotype in Glu-A1 and Glu-D1 and single null genotype in Glu-D1 showed significantly higher cookie diameter, crispness, and lower cookie height compared with single null genotype in Glu-A1 and WT. These indicate that the null Glu-D1 genotype is useful for improvement of biscuit quality, and use of this germplasm would be a viable strategy to develop new wheat varieties for biscuit processing.

关键词： wheat HMW-GS deletion near-isogenic lines cookie quality

基于残差预测修正的局部在线时间序列预测方法

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电子学报 2008年第B12期36卷 81-85页

作者：刘大同彭宇彭喜元哈尔滨工业大学自动化测试与控制研究所黑龙江哈尔滨150080

对于复杂的非线性和非平稳时间序列预测,基本的支持向量回归(Support Vecotr Regression,SVR)在线算法无法有效兼顾执行效率和预测精度.本文首先采用局部SVR进行时间序列建模预测,同步计算在线更新序列数据预测的残差,并采用Online SVR... 详细信息

对于复杂的非线性和非平稳时间序列预测,基本的支持向量回归(Support Vecotr Regression,SVR)在线算法无法有效兼顾执行效率和预测精度.本文首先采用局部SVR进行时间序列建模预测,同步计算在线更新序列数据预测的残差,并采用Online SVR对残差序列进行混沌时间序列预测,将预测残差值实时补偿到局部SVR模型预测输出.实验结果表明,新方法在执行效率和预测精度方面较单一Online SVR均显著提高.

关键词：时间序列预测在线预测 SVR 残差

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大型胶带输送机断带保护技术

煤矿开采 2010年第1期15卷 77-78,80页

作者：刘大同大同煤矿集团有限责任公司技术中心山西大同037003

介绍了国外胶带输送机钢绳芯受损情况检测器——"CBM检测器"与国内铁磁材料及X射线检测胶带输送机钢绳芯受损技术。介绍了断带保护装置KHP119型抓捕机构工作原理及应用。对目前现场使用的胶带输送机钢绳芯受损检测技术,提出研究方向和... 详细信息

介绍了国外胶带输送机钢绳芯受损情况检测器——"CBM检测器"与国内铁磁材料及X射线检测胶带输送机钢绳芯受损技术。介绍了断带保护装置KHP119型抓捕机构工作原理及应用。对目前现场使用的胶带输送机钢绳芯受损检测技术,提出研究方向和应用建议。

关键词：胶带输送机断带保护应用建议

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作物籽粒中水分运输路径及其可视化研究进展

植物生理学报 2017年第11期53卷 1947-1954页

作者：刘大同王忠闫大伟熊飞江苏里下河地区农业科学研究所/农业部长江中下游小麦生物学与遗传育种重点实验室江苏扬州225007 扬州大学江苏省作物遗传生理重点实验室/粮食作物现代产业技术协同创新中心江苏扬州225009 剑桥大学塞恩斯伯里实验室

植物体内水分运输是各器官密切联系和各种生理代谢有序进行的根本保障。对水分含量高低和运输路径进行可视化,在无损条件下对植物体内水分运输结构进行成像分析,是多学科多技术交叉研究的热点。目前对谷物籽粒中水分排出的机制和路径研... 详细信息

植物体内水分运输是各器官密切联系和各种生理代谢有序进行的根本保障。对水分含量高低和运输路径进行可视化,在无损条件下对植物体内水分运输结构进行成像分析,是多学科多技术交叉研究的热点。目前对谷物籽粒中水分排出的机制和路径研究非常欠缺,有关小麦(Triticum aestivum)穗部各组织的维管束结构和走向,尤其是颖果和穗部维管束的联络关系等尚存疑问。本文综述了植物体内水分运输路径可视化研究策略和技术的进展,并结合多年研究基础预测了小麦颖果中水分排出的方式,对研究中存在的问题进行了展望。

关键词：水分运输路径小麦可视化维管束系统 micro-CT

Micro-strain regulation strategy to stabilize perovskite lattice based on the categories and impact of strain on perovskite solar cells

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Journal of Energy Chemistry 2025年第1期100卷 578-604页

作者： Caixia Li Wenwu liu Shiji da Lingbin Kong Fen Ran State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals Lanzhou University of Technology School of Materials Science and Engineering Lanzhou University of Technology

Photovoltaic metal halide perovskite solar cells(PSCs) convert light to electricity more efficiently than crystalline silicon cells, and the cost of materials used to make them is lower than that of silicon *** efficiency is not a core issue affecting the application of perovskite solar cells in special *** present, stability is the major technical encounters that hinders its further commercial development. Microstrain in PSCs is currently a significant factor responsible for the device's instability. Strain-induced ion migration is widely believed to accelerate perovskite degradation even when external stimuli are ***, it is imperative to study strain to enhance the stability of PSCs. This paper reviews recent developments to understand strain's origin and effect mechanisms on performance of PSCs, including ion migration,failure behavior, defect formation, and its effect on photoelectric properties, stability, and ***, several well-known strain management strategies are systematically introduced based on the strain effect mechanism and strain engineering on the film, providing more clues for further preparation with increased stability. The manipulation of external physical strain applied from films to entire devices has been extensively studied. Furthermore, recommendations for future research directions and chemical approaches have been provided. It is emphasized that strain engineering plays a crucial role in improving the efficiency and longevity of PSCs. Tensile strain causes rapid degradation, while moderate compressive strain and external strain control could improve properties and stability. Efforts should focus on controlling compressive strain to mitigate residual tensile strain and introducing it in a controlled manner. Future research endeavors may focus on exploring these pathways to improve the efficiency and lifespan of PSCs.

关键词： Halide perovskite solar cells Strain origins Strain effects Strain management