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食品基碳量子点的制备、生物活性及其在食品包装中的应用研究进展

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食品安全质量检测学报 2024年第7期15卷 113-121页

作者：韩天奇任大勇牛海月张程程刘大群忻晓庭章检明吉林农业大学食品科学与工程学院长春130000 浙江省农业科学院农业农村部蔬菜采后保鲜与加工重点实验室(部省共建)浙江省果蔬保鲜与加工技术研究重点实验室食品科学研究所杭州310000

碳量子点是一种尺寸小于10nm的新型碳纳米材料,因其优异的光学特性受到国内外学者的广泛关注。目前用于合成碳量子点的食品主要来自果蔬及其制品、乳及乳制品、肉及肉制品和水产品及其制品等。一步水热合成法是制备食品基碳量子点最常... 详细信息

碳量子点是一种尺寸小于10nm的新型碳纳米材料,因其优异的光学特性受到国内外学者的广泛关注。目前用于合成碳量子点的食品主要来自果蔬及其制品、乳及乳制品、肉及肉制品和水产品及其制品等。一步水热合成法是制备食品基碳量子点最常用的方法。因此,食品基碳量子点相比于化学材料合成的碳量子点具有易于合成、价格低廉、绿色无毒、生物相容性好等优势。并且食品基碳量子点表面通常带有丰富的官能团与杂元素,赋予了碳量子点更优异的抗癌、抗氧化和抗菌等功能活性。目前在生物医学、功能性材料、食品检测等领域广泛应用。近年来,由于食品基碳量子点优异的抗氧化、抗菌和光学活性已被添加到食品包装系统中,增强食品包装紫外屏蔽、抗氧化和抗菌等性能。本文综述了以食品为原料的碳量子点制备方法和原料来源,探讨了食品基碳量子点的优势及其生物活性,以及添加食品基碳量子点的活性包装在果蔬等食品保鲜中的应用,为食品碳量子点的绿色制备及其在活性包装中的应用提供参考和理论依据。

关键词：食品基碳量子点制备生物活性食品包装

Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat

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Agricultural Sciences in China 2011年第12期10卷 1898-1905页

作者： ZHANG Na YANG Wen-xiang liu da-qun Department of Plant Pathology/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province Agricultural University ofHebei Baoding 071001 P.R.China

This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection （MAS）. An F2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker. A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk. Four AFLP markers, P-AGA/M-CTT289 bp, P-AGC/M-CAC1ss bp, P-AGC/M- CAC162 bp, and P-ACG/M-CGC239 bp, were co-segregated with Lr24. The AFLP fragment from the primer combination P- ACG/M-CGC was cloned, sequenced and converted into a STS marker named as ASTS212. Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS13026oz developed by Gupta. 5R615, 5R616, IR13, and 1R17 were identified and validated to contain gene Lr24. The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.

关键词： wheat leaf rust Lr24 gene AFLP STS MAS

TaRAR1 is Required for Lr24-Mediated Wheat Leaf Rust Resistance

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Agricultural Sciences in China 2011年第11期10卷 1732-1738页

作者： ZHANG Li-rong YANG Wen-xiang liu da-qun College of Plant Protection Biological Control Center of Plant Diseases and Pests of Hebei Province Agricultural University of HebeiBaoding 071001 P.R.China

Virus-induced gene silencing （VIGS） offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus （BSMV） VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RT- PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.

关键词： disease resistance barley stripe mosaic virus (BSMV) virus-induced gene silencing (VIGS) Lr24 RAR1

Isolation and Characterization of NBS-LRR Class Resistance Homologous Gene from Wheat

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Agricultural Sciences in China 2011年第8期10卷 1151-1158页

作者： ZHANG Nan WANG Shen WANG Hai-yan liu da-qun College of Plant Protection/Biological Control Center of Plant Disease and Plant Pests of Hebei Province Agricultural University of HebeiBaoding 071001P.R.China

One resistance gene analog fragment named RGA-CIN14 was isolated from TcLr19 wheat,which contains kinase-2,kinase-3a,and the GLPL motif of the NBS-spanning region,using degenerated primers according to the nucleotide binding site （NBS） conserved *** on the RGA-CIN14,a full-length cDNA,CIN14,which was 2 987 bp encoding 880 amino acids,was obtained by using the method of the rapid amplification cDNA ends （RACE）.Bioinformatics analysis showed that the deduced amino acids of CIN14 protein consisted of a NB-ARC conserved domain and many leucine-rich repeats （LRR） *** phylogenetic tree analysis indicated a considerable identity of the protein encoded by CIN14 with that of wheat leaf rust resistance gene Lr1,but a lower similarity with *** expression profile of the CIN14 gene detected by semi-quantitative RT-PCR showed that the CIN14 gene was not induced by Puccinia triticina and it was a constitutive gene with low abundance in the wheat leaf *** resistance homology sequence was successfully obtained,which provides the shortcut for cloning of the resistance gene in TcLr19 wheat.

关键词： wheat leaf rust resistance gene NBS-LRR resistance gene analogs (RGAs) rapid amplification cDNA end (RACE) RT-PCR

Procyanidin A_1 and its digestive products alleviate acrylamide-induced IPEC-J2 cell damage through regulating Keap1/Nrf2 pathway

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Food Science and Human Wellness 2024年第3期13卷 1475-1484页

作者： Fangfang Yan qun Lu Chengming Wang Rui liu College of Food Science and Technology Huazhong Agricultural UniversityWuhan 430070China Key Laboratory of Environment Correlative Dietology(Huazhong Agricultural University) Ministry of EducationWuhan 430070China Key Laboratory of Urban Agriculture in Central China Ministry of Agriculture and Rural AffairsWuhan 430070China

Our previous study has revealed that procyanidin A_(1)(A_(1))and its simulated digestive product(D-A,)can alleviate acrylamide(ACR)-induced intestine cell ***,the underlying mechanism remains *** this study,we elucidated the molecular mechanism for and D-A_(1) to alleviate ACR-stimulated IPEC-J2 cell *** slightly activated nuclear factor erythroid 2-related factor 2(Nrf2)signaling and its target genes,but this activation could not reduce intestine cell damage.A_(1) and D-A_(1) could alleviate ACR-induced cell damage,but the effect was abrogated in cells transiently transfected with Nrf2 small interfering RNA(siRNA).Further investigation confirmed that A_(1) and D-A_(1) interacted with Ketch-like ECH-associated protein 1(Keapl),which boosted the stabilization of Nrf2,subsequently promoted the translocation of Nrf2 into the nucleus,and further increased the expression of antioxidant proteins,thereby inhibiting glutathione(GSH)consumption,maintaining redox balance and eventually alleviating ACR-induced cell ***,there was no difference between A_(1) and D-A_(1) treated groups,indicating that A_(1) can tolerate gastrointestinal digestion and may be a potential compound to limit the toxicity of ACR.

关键词： Procyanidin A_1 Digestive products Acrylamide Nuclear factor erythroid 2-related factor 2(Nrf2) Intestinal cell damage

Identification of leaf rust resistance genes in common wheat varieties from China and foreign countries

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Journal of Integrative Agriculture 2021年第5期20卷 1302-1313页

作者： liu Yuan Takele Weldu GEBREWAHID ZHANG Pei-pei LI Zai-feng liu da-qun College of Plant Protection Hebei Agricultural UniversityBaoding 071001P.R.China College of Agriculture Aksum UniversityShire-Indaslassie 314Ethiopia

Wheat leaf rust,triggered by Puccinia triticina Eriks(Pt),is among the most important diseases of wheat *** resistant varieties against leaf rust is the most effective,environmentally-friendly and economic way to control the *** the present study,66 wheat varieties form China and foreign countries were tested with 17 Pt races for gene postulation during the seedling stage in the *** the varieties were also planted to identify slow rusting responses to leaf rust at the adult plant stage in Baoding and Zhoukou field trials during the 2016/2017 to 2017/2018 cropping ***,12 closely linked molecular markers to known leaf rust resistance(Lr)genes were used for assessing all the *** results of both gene postulation and molecular marker identification showed that a total of eight Lr genes,Lr1,Lr10,Lr17,Lr20,Lr26,Lr34,Lr37 and Lr46,either singly or in combination were detected in 32 *** Lr genes were not identified in the remaining 34 *** varieties were found to have slow rusting *** resistance sources identified in this study can be used as resources for resistance against leaf rust in wheat breeding programs in China and the respective foreign countries.

关键词： wheat gene postulation molecular marker Lr gene

Balanced pairs and relative tilting objects in recollements of abelian categories

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Acta Mathematica Sinica,English Series 2024年

作者： Pei Yu ZHANG Meng Hui liu da Jun liu Jia qun WEI School of Mathematics-Physics and Finance Anhui Polytechnic University Department of Mathematics Zhejiang Normal University

In this paper, we study the relationship of balanced pairs in a recollement. As an application of balanced pairs, we introduce the notion of the relative tilting objects, and give a characterization of relative tilting objects, which is similar to Bazzoni characterization of n-tilting modules. Finally, we investigate the relationship of relative tilting objects in a recollement.

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Cause analysis of expressway traffic accidents based on accident statistics of Hunan provincial expressway in 2012-2018

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Transportation Safety and Environment 2024年第1期6卷 83-92页

作者： Xinyu liu Shengjun Huang qun Chen Business school Foshan UniversityFoshan 528225GuangdongChina School of Traffic and Transportation Engineering Central South UniversityChangsha 410075HunanChina Hunan Provincial Expressway Traffic Police Station Law Enforcement DepartmentChangsha 410007HunanChina

Based on the 2012–2018 accident statistics on the Hunan Provincial Expressway in China,this paper analyses the proportion to which various factors contribute to accidents,as well as the trends over *** 2012 to 2018,the total number of general procedure handling accidents(*** accidents)increased first and then decreased,while the number of accidents and deaths per 1000 kilometres decreased year by *** total number of simple procedure handling accidents(*** accidents)increased year by year,but the number of accidents per 1000 kilometres remained relatively ***,fatigued driving,improper driving operation,failure to maintain a safe distance from the preceding vehicle and pedestrians entering the expressway are the main causes of general procedure handling *** to maintain a safe distance from the preceding vehicle as required and improper driving operation accounted for approximately 2/3 of the simple procedure handling *** view of the accidents caused by these factors,how to use law enforcement,education,science and technology and facilities construction to further reduce the occurrence of expressway traffic accidents is discussed.

关键词： expressway traffic accident cause analysis

Microbial phenolic metabolites 3-(3',4'-dihydroxyphenyl)propanoic acid and 3',4'-dihydroxyphenylacetic acid prevent obesity in mice fed high-fat diet

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Food Science and Human Wellness 2024年第1期13卷 327-338页

作者： Wanbing Chen Ruonan liu Xiaoling Zhu qun Lu Hong Yang Rui liu College of Food Science and Technology Huazhong Agricultural UniversityWuhan 430000China Key Laboratory of Environment Correlative Dietology(Huazhong Agricultural University) Ministry of educationWuhan 430000China Wuhan Engineering Research Center of Bee Products on Quality and Safety Control Wuhan 430000China Key Laboratory of Urban Agriculture in Central China Ministry of Agriculture and Rural AffairsWuhan 430000China Hubei Provincial Institute for Food Supervision and Test Wuhan 430070China

Obesity is associated with numerous metabolic disorders,and dietary polyphenols have been confirmed to have beneficial effects on the metabolism in ***,the effect of 3-(3’,4’-dihydroxyphenyl)propanoic acid(DHPA)and 3’,4’-dihydroxyphenylacetic acid(DHAA),two main metabolites of dietary polyphenols,on obesity remains poorly *** this study,DHPA and DHAA were found to alleviate obesity,as well as regulate insulin resistance,lipid metabolism,and oxidative stress response in high-fat diet(HFD)***,the 16S rRNA sequencing and UHPLC-Q-TOF/MS demonstrated that DHPA and DHAA only slightly disturbed the intestinal microbiome,but significantly altered the urine metabolome of HFD mice mainly by regulating pentose and glucuronate interconversion,tyrosine metabolism,pentose phosphate and tricarboxylic acid(TCA)cycle as indicated by metabolic pathway analysis based on Kyoto Encyclopedia of Genes and Genomes(KEGG)*** analysis revealed that the differential metabolites are strongly associated with body weight,blood glucose,insulin level,and superoxide dismutase(SOD)enzyme *** results revealed that DHPA and DHAA exert their anti-obesity effect by regulating important metabolites in the glucose,lipid and tyrosine metabolism pathways.

关键词： Obesity Dietary polyphenol metabolites Gut microbiota Metabonomics