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Comparative transcriptome study provides insights into acquisition of embryogenic ability in upland cotton during somatic embryogenesis

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Journal of Cotton Research 2018年第2期1卷 44-56页

作者： SUN Ruibin TIAN Ruiping MA Dan WANG Shaohui liu chuanliang State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agriculture Sciences Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou University

Background： The conversion from non-embryogenic callus （NEC） to embryogenic callus （EC） is the key bottleneck step in regeneration of upland cotton （Gossypium hirsutum）, and hinders the transgenic breeding of upland cotton. To investigate molecular mechanisms underlying acquisition of embryogenic potential during this process, comparation analysis of transcriptome dynamics between two upland cotton cultivars with different somatic embryogenesis abilities was conducted. Results： Differentially expressed genes involved in the transformation from NEC to EC were detected in the two different cultivars. Principal component analysis based on DEGs showed that the NEC tissues of the two cultivars were highly heterogeneous, whereas the derived EC tissues were similar, which suggested the homogeneousness of EC between different lines. In the highly embryogenic cultivar CCRI 24, more of these genes were down-regulated, whereas, in the recalcitrant cultivar CCRI 12, more were up-regulated. Bioinformatics analysis on these DEGs showed that the vast majority of differentially expressed genes were enriched in metabolism and secondary metabolites biosynthesis pathways. Flavonoid biosynthesis and phenylpropanoid biosynthesis pathways were enriched in both cultivars, and the associated genes were down-regulated more in CCRI 24 than in CCRI 12. We deduced that vigorous secondary metabolism in CCRI 12 may hinder primary metabolism, resulting in tardiness of cell differentiation. Interestingly, genes involved in the plant hormone signal transduction pathway were enriched in the recalcitrant cultivar CCRI 12, but not in CCRI 24, suggesting more radical regulation of hormone signal transduction in the recalcitrant cultivar. Signal transduction rather than biosynthesis of plant hormones is more likely to be the determining factor triggering NEC to EC transition in recalcitrant cotton lines. Transcription factor encoding genes showed differential regulation between two cultivars. Conclusion

关键词： Upland cotton Transcriptome Non-embryogenic callus (NEC) Embryogenic callus (EC) Somatic embryogenesis

Transcriptome Profiling Reveals Auxin and Cytokinin Regulating Somatic Embryogenesis in Different Sister Lines of Cotton Cultivar CCRI24

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Journal of Integrative Plant Biology 2013年第7期55卷 631-642页

作者： Zhenzhen Xu Chaojun Zhang Xueyan Zhang chuanliang liu Zhixia Wu Zuoren Yang Kehai Zhou Xiaojie Yang Fuguang Li State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agriculture Sciences

To get a broader view on the molecular mechanisms underlying somatic embryogenesis （SE） in cotton （Gossypium hirsutum L.）, global analysis of cotton transcriptome dynamics during SE in different sister lines was performed using RNA-Seq. A total of 204 349 unigenes were detected by de novo assembly of the 214 977 462 Illumina reads. The quantitative reverse transcription-polymerase chain reaction （qRT-PCR） measurements were positively correlated with the RNA-Seq results for almost all the tested genes （R2 -- 0.841, correlation was significant at the 0.01 level）. Different phytohormone （auxin and cytokinin） concentration ratios in medium and the endogenous content changes of these two phytohormones at two stages in different sister lines suggested the roles of auxin and cytokinin during cotton SE. On the basis of global gene regulation of phytohormone-related genes, numerous genes from all the differentially expressed transcripts were involved in auxin and cytokinin biosynthesis and signal transduction pathways. Analyses of differentially expressed genes that were involved in these pathways revealed the substantial changes in gene type and abundance between two sister lines. Isolation, cloning and silencing/ overexpressing the genes that revealed remarkable up- or down-expression during cotton SE were important. Furthermore, auxin and cytokinin play a primary role in SE, but potential cross-talk with each other or other factors remains unclear.

关键词： Auxin cotton cytokinin RNA-Seq somatic embryogenesis.

SINAT E3 ligases regulate the stability of the ESCRT component FREE1 in response to iron deficiency in plants

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Journal of Integrative Plant Biology 2020年第9期62卷 1399-1417页

作者： Zhidan Xiao Chao Yang chuanliang liu Lianming Yang Shuhong Yang Jun Zhou Faqiang Li Liwen Jiang Shi Xiao Caiji Gao Wenjin Shen Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life SciencesSouth China Normal UniversityGuangzhou510631China MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou510631China College of Life Sciences South China Agricultural UniversityGuangzhou510642China School of Life Sciences Center for Cell&Developmental Biology and State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongNew TerritoriesHong KongChina State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-sen UniversityGuangzhou510275China

The endosomal sorting complex required for transport (ESCRT) machinery is an ancient, evolutionarily conserved membrane remodeling complex that is es-sential for multivesicular body (MVB) biogenesis in eu-karyotes. FYVE DOMAIN PROTEIN REQUIRED FOR EN-DOSOMAL SORTING 1 (FREE1), which was previously identified as a plant-specific ESCRT component, modu-lates MVB-mediated endosomal sorting and autophagic degradation. Although the basic cellular functions of FREE1 as an ESCRT component have been described, the regulators that control FREE1 turnover remain unknown. Here, we analyzed how FREE1 homeostasis is mediated by the RING-finger E3 ubiquitin ligases, SINA of Arabi-dopsis thaliana (SINATs), in response to iron deficiency. Under iron-deficient growth conditions, SINAT1-4 were induced and ubiquitinated FREE1, thereby promoting its degradation and relieving the repressive effect of FREE1 on iron absorption. By contrast, SINAT5, another SINAT member that lacks ubiquitin ligase activity due to the absence of the RING domain, functions as a protector protein which stabilizes FREE1. Collectively, our findings uncover a hitherto unknown mechanism of homeostatic regulation of FREE1, and demonstrate a unique regu-latory SINAT–FREE1 module that subtly regulates plant response to iron deficiency stress.

关键词： component sorting thereby

Tumor immunological phenotype-derived gene classification predicts prognosis,treatment response,and drug candidates in ovarian cancer

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Genes & Diseases 2024年第5期11卷 94-97页

作者： Chengbin Guo Yuqin Tang Zhihai liu a chuanliang Chen Xun Hu Yongqiang Zhang Guangzhou Women and Children’s Medical Center Guangzhou Medical UniversityGuangzhouGuangdong 510623China Clinical Bioinformatics Experimental Center Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan 450003China School of Pharmacy Macao University of Science and TechnologyTaipaMacao 999078China Clinical Research Center The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310003China Biorepository State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan 610041China West China School of Medicine West China HospitalSichuan UniversityChengduSichuan 610041China

The high recurrence and low responsiveness to immunotherapy make ovarian cancer(OC)the most lethal gynecological *** microenvironment is critical in risk stratification and the discovery of molecular *** developed a prognostic classification for OC,which could also predict the prognosis of other gynecological cancers including breast cancer,endometrial cancer,and cervical *** mutation,hallmark pathways,and immune landscapes were *** analysis of immune checkpoints and multiple immune signatures revealed the low-risk group responds better to immune checkpoint inhibitors,which was validated by an external immunotherapeutic cohort(IMvigor210).Singlecell RNA sequencing(scRNA-seq)confirmed the high expression of SERPINB1 and SERPINB9 in dendritic cells,and AlphaFold2 was used to infer their 3D protein *** molecular compounds binding to SERPINB1/SERPINB9 were predicted by virtual screening.

关键词： prognosis cancer treatment

CFB锅炉钙基飞灰的雾化喷水悬浮式脱硫特性

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环境工程 2022年第7期40卷 81-87页

作者：刘传亮上海发电设备成套设计研究院有限责任公司上海200240

以循环流化床(CFB)锅炉钙基飞灰为原料,实验研究了不同条件下飞灰的雾化喷水悬浮式脱硫工艺技术特性。结果表明:增湿飞灰具有良好的低温脱硫能力,其脱硫过程分为快速、慢速反应2个阶段,增湿前后脱硫剂的总钙利用率从41%提高到70%左右。... 详细信息

以循环流化床(CFB)锅炉钙基飞灰为原料,实验研究了不同条件下飞灰的雾化喷水悬浮式脱硫工艺技术特性。结果表明:增湿飞灰具有良好的低温脱硫能力,其脱硫过程分为快速、慢速反应2个阶段,增湿前后脱硫剂的总钙利用率从41%提高到70%左右。飞灰颗粒的增湿效果与反应温度是影响硫盐化速率的主要因素。采用50μm雾化水粒径、增湿水分级喷入方式,可使飞灰颗粒获得更好的增湿效果,并延长快速反应的持续时间,使反应更充分;反应温度对增湿脱硫同时存在促进与抑制2方面作用,最佳反应温度在80℃左右;SO_(2)浓度对脱硫的影响不显著;飞灰经过雾化喷水活化后,颗粒表面的孔隙、裂缝增多,改善的微观结构促进了气固传质和脱硫反应。

关键词：脱硫钙基吸收剂飞灰雾化喷水水活化循环流化床

ABI5–FLZ13 module transcriptionally represses growth-related genes to delay seed germination in response to ABA

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Plant Communications 2023年第6期4卷 277-295页

作者： Chao Yang Xibao Li Shunquan Chen chuanliang liu Lianming Yang Kailin Li Jun Liao Xuanang Zheng Hongbo Li Yongqing Li Shaohua Zeng Xiaohong Zhuang Pedro L.Rodriguez Ming Luo Ying Wang Caiji Gao Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement&Guangdong Provincial Key Laboratory of Applied Botany South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life SciencesSouth China Normal University(SCNU)Guangzhou 510631China School of Life Sciences Centre for Cell and Developmental BiologyThe Chinese University of Hong KongShatinNew TerritoriesHong KongChina Instituto de Biologia Molecular y Celular de Plantas Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia46022 ValenciaSpain

The bZIP transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)is a master regulator of seed germination and post-germinative growth in response to abscisic acid(ABA),but the detailed molecularmechanism by which it represses plant growth remains *** this study,we used proximity labeling to map the neighboring proteome of ABI5 and identified FCS-LIKE ZINC FINGER PROTEIN 13(FLZ13)as a novel ABI5 interaction *** analysis of flz13 mutants and FLZ13-overexpressing lines demonstrated that FLZ13 acts as a positive regulator of ABA *** analysis revealed that both FLZ13 and ABI5 downregulate the expression of ABA-repressed and growth-related genes involved in chlorophyll biosynthesis,photosynthesis,and cell wall organization,thereby repressing seed germination and seedling establishment in response to *** genetic analysis showed that FLZ13 and ABI5 function together to regulate seed ***,our findings reveal a previously uncharacterized transcriptional regulatorymechanismby which ABA mediates inhibition of seed germination and seedling establishment.

关键词： ABA ABI5 FLZ gene expression seed germination

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T/R组件小型化脉冲电源的研制

现代雷达 2018年第5期40卷 66-68,89页

作者：刘传亮连云港杰瑞电子有限公司

在防御体系中相控阵雷达获得越来越广泛的应用，其内部T／R组件工作时需要很大的脉冲功率，要求其供电模块具有动态响应速度快、体积小、功率密度高等特点。首先，文中研究了一种两级式电路拓扑：其前级为全桥开环隔离变换电路，实现电... 详细信息

在防御体系中相控阵雷达获得越来越广泛的应用，其内部T／R组件工作时需要很大的脉冲功率，要求其供电模块具有动态响应速度快、体积小、功率密度高等特点。首先，文中研究了一种两级式电路拓扑：其前级为全桥开环隔离变换电路，实现电气隔离及母线降压；后级为同步整流Buck电路，实现稳定的输出电压；然后，依据实际的应用需求，给出了电路设计思路、关键参数的设计准则以及主要器件选型；最后，给出了电路仿真和试验结果，验证了理论分析和参数设计的准确性。

关键词：两级式变换器脉冲电源发射/接收组件

Age-sPecific PSA reference ranges in Chinese Men without Prostate Cancer

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Age-sPecific PSA reference ranges in Chinese Men without Pro...

Efficify and satefy of treating premature ejaculation with 4weeks Sertraline

第十六届全国泌尿外科学术会议

作者： Zhiyong liu,Yinghao Sun,chuanliang Xu,Luoman Zhang,Xu Gao,Shancheng Ren 上海长海医院泌尿外科

Objectives:To determine the age-sPecific Prostate-sPecific antigen(PSA) distributions in Chinese men without Prostate cancer and to recommend the age-sPecific PSA reference ranges for Chinese men after comParing these...

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Efficify and satefy of treating premature ejaculation with 4...