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Modified Si-Miao-San ameliorates pancreatic B cell dysfunction by inhibition of reactive oxygen species-associated inflammation through AMP-kinase activation

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Chinese Journal of Natural Medicines 2014年第5期12卷 351-360页

作者： SHANG Shu-Wan YANG Jiang-lin HUANG Fang liu Kang liu bao-lin State key laboratory of Natural Medicines Department of Pharmacology of Chinese Materia Medica China Pharmaceutical University Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Nanjing University of Chinese Medicines

AIM: To observe the effect of modified Si-Miao-San(mSMS) on advanced glycation end products(AGEs)-induced pancreatic B cell dysfunction, as well as examining the underlying mechanisms. METHOD: Pancreatic B cells(INS-1) were stimulated with advanced glycation end products(AGEs, 200 μg·mL-1) for 24 h to produce dysfunction in pancreatic B cells and the effects of mSMS observed on insulin secretion, NF-κB(p65) phosphorylation, reactive oxygen species(ROS) production, mitochondria membrane potential(Δψm), cell apoptosis, phosphorylation of AMP-kinase(AMPK), and caspase 3 activity. RESULTS: The AGEs challenge resulted in increased basal insulin secretion, but decreased insulin secretion in response to high glucose, whereas this situation was reversed by mSMS treatment. AGEs stimulation induced NF-κB(p65) phosphorylation and reactive oxygen species(ROS) production, as well as Δψm collapse and cell apoptosis. mSMS inhibited ROS production and inhibited NF-κB activation by attenuating p65 phosphorylation. Meanwhile, AGEs-induced Δψm collapse and cell apoptosis were also reversed by mSMS treatment. Compound C, an inhibitor of AMP-Kinase(AMPK), abolished the beneficial effects of mSMS on the regulation of B cell function, indicating the involvement of AMPK. CONCLUSION: mSMS ameliorated AGEs-induced B cell dysfunction by suppressing ROS-associated inflammation, and this action was related to its beneficial regulation of AMPK activity.

关键词：药理学天然药物性质实验药理学

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Modified Si-Miao-San inhibits inflammation and promotes glucose disposal in adipocytes through regulation of AMP-kinase

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Chinese Journal of Natural Medicines 2014年第12期12卷 911-919页

作者： YANG Jiang-lin WANG Jun-Lian HUANG Fang liu Kang liu bao-lin Department of Pharmacology of Chinese Materia Medica China Pharmaceutical University

Modified Si-Miao-San(mS MS) is composed of Rhizoma Coptidis, Cortex Phellodendri, Rhizoma Coptidis Semen Coicis and Atractylodes Rhizome. The prescription is used for the management of diabetes and insulin resistance in the clinic. This study aims to investigate its regulation of glucose disposal in adipocytes. Differentiated 3T3-L1 adipocytes were stimulated with conditioned medium derived from activated macrophages to induce insulin resistance and observed the effects of Mac-CM on insulin-mediated glucose uptake along the insulin receptor substrate-1/PI3K/Akt signaling pathway. Moreover, its regulation of AMPK phosphorylation was also investigated. mS MS enhanced AMPK phosphorylation and promoted basal glucose uptake in adipocytes; mS MS inhibited NF-κB activation by reducing P65 phosphorylation and improved insulin-stimulated IRS-1 tyrosine and Akt phosphorylation, leading to the restoration of insulin-mediated glucose uptake when cells were exposed to inflammatory stimulation. These beneficial effects were diminished in the presence of the AMPK inhibitor compound C. mS MS positively regulated AMPK activity, and this action contributed to improving insulin PI3 K signaling by the beneficial regulation of IRS-1 function through inhibition of inflammation in adipocytes.

关键词： Modified Si-Miao-San AMP-kinase Inflammation Insulin resistance Adipocytes

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Mangiferin ameliorates insulin resistance by inhibiting inflammation and regulatiing adipokine expression in adipocytes under hypoxic condition

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Chinese Journal of Natural Medicines 2017年第9期15卷 664-673页

作者： YANG Chao-Qiang XU Jing-Hua YAN Dan-Dan liu bao-lin liu Kang HUANG Fang Department of Pharmacology of Chinese Materia Medicine China Pharmaceutical University Nanjing 211198 China

Adipose tissue hypoxia has been recognized as the initiation of insulin resistance syndromes. The aim of the present study was to investigate the effects of mangiferin on the insulin signaling pathway and explore whether mangiferin could ameliorate insulin resistance caused by hypoxia in adipose tissue. Differentiated 3 T3-L1 adipocytes were incubated under normal and hypoxic conditions, respectively. Protein expressions were analyzed by Western blotting. Inflammatory cytokines and HIF-1-dependent genes were tested by ELISA and q-PCR, respectively. The glucose uptake was detected by fluorescence microscopy. HIF-1α was abundantly expressed during 8 h of hypoxic incubation. Inflammatory reaction was activated by up-regulated NF-κB phosphorylation and released cytokines like IL-6 and TNF-α. Glucose uptake was inhibited and insulin signaling pathway was damaged as well. Mangiferin substantially inhibited the expression of HIF-1α. Lactate acid and lipolysis, products released by glycometabolism and lipolysis, were also inhibited. The expression of inflammatory cytokines was significantly reduced and the damaged insulin signaling pathway was restored to proper functional level. The glucose uptake of hypoxic adipocytes was promoted and the dysfunction of adipocytes was relieved. These results showed that mangiferin could not only improve the damaged insulin signaling pathway in hypoxic adipocytes, but also ameliorate inflammatory reaction and insulin resistance caused by hypoxia.

关键词： Mangiferin 组织缺氧发炎胰岛素抵抗胰岛素发信号小径

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Effects of Hydroxyapatite Nanoparticles on Devitrification Crystallization in Cryoprotectant Solutions

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工程热物理学报 2015年第1期36卷 142-146页

作者： LV Fukou liu bao-lin LI Wei-Jie Ganesh K.Jaganathan Institute of Biothermal Science and Technology University of Shanghai for Science and technology

To study the effects of Hydroxyapatite(HA) nanoparticles on devitrification in cryoprotectant solutions,the crystallization of glycerol(60%,w/w) and PEG-600(50%,w/w) solutions with HA nanoparticles of different sizes(20 nm、 40 nm、60 nm)and different concentrations(0.1%、 0.5%)during warming were investigated by using differential scanning calorimetry(DSC) combined with cryomicroscopy.Experimental results showed that the presence of nanoparticles does not change the glass transition temperatures and melting temperatures of quenched solutions,but affects the behavior of devitrification and recrystallization upon warming.The morphologies of glycerol and PEG-600 solutions are dendritic and spheric respectively,and the structures are not changed by adding nanoparticles.The ice fraction of glycerol solution containing 0.1%60nm HA nanoparticles diminished significantly when comparing to the control solution.The ice fractions of PEG-600 solutions increased dramatically between-64℃ and-54℃.The findings have significant implications for biomaterial cryopreservation.

关键词： nanoparticles devitrification crystallization DSC cryomicroscope

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Abdominal separation in an adult male patient with acute abdominal pain

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World Journal of Gastroenterology 2010年第26期16卷 3343-3346页

作者： liu, bao-lin Chen, Yang liu, Shi-Qing Zhang, Xiao-Bo Cui, Dong-Xu Dai, Xian-Wei China Med Univ Shengjing Hosp Dept Surg Oncol & Organ Transplatat Div Shenyang 110004 Liaoning Prov Peoples R China

We report a male patient with prolonged post-prandial abdominal distension and a sudden onset of epigastric pain initially diagnosed as acute abdomen. The patient had no history of surgery. Physical examination revealed peritonitis and abdominal computed tomography scan showed upper abdominal mesentery intorsion. The patient then underwent surgical intervention. It was found that the descending mesocolon dorsal root was connected to the ascending colon and formed a membrane encapsulating the small intestine. The membrane also formed an orifice in the ileal pars caeca, from which a 30 cm herniated ileum formed a "C"-shaped loop which was strangulated by the orifice. An abdominal separation was diagnosed after surgery. We liberated the membranous peritoneum which incarcerated the intestinal canal from the root ofileocecal junction to Treitz ligament, and reduced the small intestinal malrotation. The patient had an uneventful recovery after opera-tion with his abdominal distention disappeared during the follow-up. Abdominal separation is a rare situation, which may be related with embryo development. Surgery is a choice of treatment for it.

关键词： Abdominal separation Abdominal pain Internal hernia Malrotation Acute abdomen

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3-D NUMERICAL SIMULATIONS OF FLOW LOSS IN HELICAL CHANNEL

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Journal of Hydrodynamics 2012年第6期24卷 858-863页

作者： ZHAO ling-zhi PENG Yan LU Fang LI Jian LI Ran liu bao-lin Institute of Electrical Engineering Chinese Academy of Sciences College of Computing and Communication Engineering University of Chinese Academy of Sciences China Ship Scientific Research Center

The flow loss of a helical channel Magnetohydrodynamic （MHD） thruster without MHD effect was numerically studied with 3-D simulations, and a flow loss coefficient （ was defined to quantify the flow loss and its influencing factors were studied. The results show that （ decreases in a first-order exponential manner with the pitch of a helical wall and the Reynolds number, and it declines slowly when t / T 〉 0.2 and Re 〉 10^5, a flow guide makes the flow more smooth and uniform, especially in the flow guide and helical wall sub-regions and thus reduces the flow loss greatly, by about 30% with the averaged value of （ from 0.0385 to 0.027, a rectifier weakens the helical flow and strengthens the axial one in the rectifier and outlet sub-regions, thus reduces the rotational kinetic pressure with the averaged value of ξ declining about 4% from 0.0385 to 0.037, and ξ decreases with a rectifier＇s axial length when Re 〉 10^5 .

关键词： helical channel Magnetohydrodynamic （MHD） propulsion rectifier flow guide flow loss

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秸秆复合管地下灌溉对冬小麦生长与水分利用效率的影响

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节水灌溉 2024年第3期 50-55,67页

作者：仵峰刘林宝宰松梅魏焕宇李欣刘丹婷华北水利水电大学水资源学院郑州450046 华北水利水电大学水利学院郑州450046 河南省节水农业重点实验室郑州450046

为了推进秸秆复合管地下灌溉技术的应用,以冬小麦为供试作物,通过田间试验,研究了秸秆复合管地下灌溉对冬小麦生长及水分利用效率的影响。结果表明,与无灌溉对照相比,秸秆复合管地下灌溉、地表滴灌和地下滴灌对冬小麦的生长与产量的提... 详细信息

为了推进秸秆复合管地下灌溉技术的应用,以冬小麦为供试作物,通过田间试验,研究了秸秆复合管地下灌溉对冬小麦生长及水分利用效率的影响。结果表明,与无灌溉对照相比,秸秆复合管地下灌溉、地表滴灌和地下滴灌对冬小麦的生长与产量的提升均有促进作用,其中秸秆复合管地下灌溉的提升效果最显著。与地表滴灌相比,秸秆复合管地下灌溉显著提升了冬小麦起身期至抽穗期的株高、开花期之后的叶面积指数及地上干物质量,冬小麦穗长和穗粒数分别增加了5.84%和9.23%,产量提升了15.55%,水分利用效率与灌溉水利用效率分别提高了21.88%与15.55%,净收益提高了77.95%。与地下滴灌相比,秸秆复合管地下灌溉提高了冬小麦返青期后的株高、叶面积指数与开花期后的地上干物质量,冬小麦穗长和穗粒数分别增加了5.15%和9.8%,产量提高了5.11%,水分利用效率与灌溉水利用效率分别提高了8.81%与5.11%,净收益提升了23.53%。秸秆复合管地下灌溉有助于促进拔节期以后冬小麦生长,提高冬小麦的产量与水分利用效率,经济效益较高,在补充灌溉区对大田密植作物具有较好的推广应用前景。

关键词：秸秆复合管地下灌溉灌溉方式冬小麦生长指标水分利用效率

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Advances in the pharmacological activities and mechanisms of diosgenin

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Chinese Journal of Natural Medicines 2015年第8期13卷 578-587页

作者： CHEN Yan TANG You-Mei YU Su-Lan HAN Yu-Wei KOU Jun-Ping liu bao-lin YU Bo-Yang Jiangsu Key Laboratory of TCM Evaluation and Translational Research Department of Complex Prescription of TCM China Pharmaceutical University

Diosgenin, a well-known steroid sapogenin derived from plants, has been used as a starting material for production of steroidal hormones. The present review will summarize published literature concerning pharmacological potential of diosgenin, and the underlying mechanisms of actions. Diosgenin has shown a vast range of pharmacological activities in preclinical studies. It exhibits anticancer, cardiovascular protective, anti-diabetes, neuroprotective, immunomodulatory, estrogenic, and skin protective effects, mainly by inducing apoptosis, suppressing malignant transformation, decreasing oxidative stress, preventing inflammatory events, promoting cellular differentiation/proliferation, and regulating T-cell immune response, etc. It interferes with cell death pathways and their regulators to induce apoptosis. Diosgenin antagonizes tumor metastasis by modulating epithelial-mesenchymal transition and actin cytoskeleton to change cellular motility, suppressing degradation of matrix barrier, and inhibiting angiogenesis. Additionally, diosgenin improves antioxidant status and inhibits lipid peroxidation. Its anti-inflammatory activity is through inhibiting production of pro-inflammatory cytokines, enzymes and adhesion molecules. Furthermore, diosgenin drives cellular growth/differentiation through the estrogen receptor(ER) cascade and transcriptional factor PPARγ. In summary, these mechanistic studies provide a basis for further development of this compound for pharmacotherapy of various diseases.

关键词： Diosgenin Steroid sapogenin Pharmacological activities Multiple targets

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Antihyperglycemic,antihyperlipidemic and antioxidant effects of standard ethanol extract of Bombax ceiba leaves in high-fat-diet-and streptozotocin-induced Type 2 diabetic rats

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Chinese Journal of Natural Medicines 2017年第3期15卷 168-177页

作者： XU Guang-Kai QIN Xiao-Ying WANG Guo-Kai XIE Guo-Yong LI Xu-Sen SUN Chen-Yu liu bao-lin QIN Min-Jian State Key Laboratory of Natural Medicines Department of Resources Science of Traditional Chinese MedicinesChina Pharmaceutical UniversityNanjing 210009China Anhui Key Laboratory of Modernized Chinese Materia Medica Anhui University of Traditional Chinese MedicineHefei 230012China Department of Pharmacology of Chinese Materia Medica China Pharmaceutical UniversityNanjing 210009China

The present study aimed at exploring the therapeutic potential of standard extract of Bombax ceiba L.leaves(BCE) in type 2 diabetic mellitus(T2DM).Oral administration of BCE at doses of 70,140,and 280 mg·kg^(-1),to the normal rats and the high-fat-diet-and streptozotocin-induced T2 DM rats were carried out.Effects of BCE on blood glucose,body weight,and a range of serum biochemical parameters were tested,and histopathological observation of pancreatic tissues was also performed.HPLC-ESI-Q/TOF-MS/MS analysis indicated that the chemical composition of BCE mainly contained mangiferin,isoorientin,vitexin,isomangiferin,isovitexin,quercetin hexoside,2'-trans-O-cumaroyl mangiferin,and nigricanside.BCE caused a significant decrease in the concentrations of fasting blood glucose,glycosylated hemoglobin,total cholesterol,triglyceride,low density lipoprotein-cholesterol,serum insulin,and malondialdehyde,and increases in oral glucose tolerance,high density lipoprotein-cholesterol,and superoxide dismutase in the T2 DM model rats.Moreover,considerable pancreatic β-cells protection effect and stimulation of insulin secretion from the remaining pancreatic β-cells could be observed after BCE treatment.The results indicated that BCE exhibited an excellent hypoglycemic activity,and alleviated dyslipidemia which is associated with T2 DM.Antioxidant activity and protecting pancreatic β-cells are the possible mechanisms involved in anti-diabetic activity of BCE.

关键词： Bombax ceiba Type 2 diabetic mellitus Antihyperglycemic Antihyperlipidemic Antioxidant

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Risk Factors of Depression Screened by Two-Sample Mendelian Randomization Analysis:A Systematic Review

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Biomedical and Environmental Sciences 2024年第1期37卷 85-95页

作者： WANG Han lin XUE Yan Feng CUI bao Qiu liu Hong SHEN Xin Xin Shandong Provincial Research Center for Bioinformatic Engineering and Technique School of Life SciencesShandong University of TechnologyZibo 255049ShandongChina Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical UniversityTaiyuan 030013ShanxiChina State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

Objective This study explored the potentially modifiable factors for depression and major depressive disorder(MDD)from the MR-Base database and further evaluated the associations between drug targets with MDD.Methods We analyzed two-sample of Mendelian randomization(2SMR)using genetic variant depression(n=113,154)and MDD(n=208,811)from Genome-Wide Association Studies(GWAS).Separate calculations were performed with modifiable risk factors from MR-Base for 1,001 genomes.The MR analysis was performed by screening drug targets with MDD in the DrugBank database to explore the therapeutic targets for MDD.Inverse variance weighted(IVW),fixed-effect inverse variance weighted(FE-IVW),MR-Egger,weighted median,and weighted mode were used for complementary calculation.Results The potential causal relationship between modifiable risk factors and depression contained 459 results for depression and 424 for MDD.Also,the associations between drug targets and MDD showed that SLC6A4,GRIN2A,GRIN2C,SCN10A,and IL1B expression are associated with an increased risk of depression.In contrast,ADRB1,CHRNA3,HTR3A,GSTP1,and GABRG2 genes are candidate protective factors against depression.Conclusion This study identified the risk factors causally associated with depression and MDD,and estimated 10 drug targets with significant impact on MDD,providing essential information for formulating strategies to prevent and treat depression.

关键词： Risk factors Drug targets Depression Major depressive disorder Two-sample Mendelian randomization

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