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circPVT1 promotes silica-induced epithelial-mesenchymal transition by modulating the miR-497-5p/TCF3 axis

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Journal of Biomedical Research 2024年第2期38卷 163-174,I0011-I0015页

作者： Siyun Zhou Yan Li wenqing Sun Dongyu Ma Yi liu Demin Cheng Guanru Li Chunhui Ni Department of Occupational Medical and Environmental Health Key Laboratory of Modern Toxicology of Ministry of EducationCenter for Global HealthSchool of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Biomedical Publications Center Nanjing Medical UniversityNanjingJiangsu 211166China Gusu School Nanjing Medical UniversityNanjingJiangsu 211166China

Epithelial-mesenchymal transition(EMT)is a vital pathological feature of silica-induced pulmonary ***,whether circRNA is involved in the process remains *** present study aimed to investigate the role of circPVT1 in the silica-induced EMT and the underlying *** found that an elevated expression of circPVT1 promoted EMT and enhanced the migratory capacity of silica-treated epithelial *** isolation of cytoplasmic and nuclear separation assay showed that circPVT1 was predominantly expressed in the *** immunoprecipitation assay and RNA pull-down experiment indicated that cytoplasmic-localized circPVT1 was capable of binding to ***,we found that miR-497-5p attenuated the silica-induced EMT process by targeting transcription factor 3(TCF3),an E-cadherin transcriptional repressor,in the silica-treated epithelial ***,these results reveal a novel role of the circPVT1/miR-497-5p/TCF3 axis in the silica-induced EMT process in lung epithelial *** validated,this finding may provide a potential theoretical basis for the development of interventions and treatments for pulmonary fibrosis.

关键词： silicosis epithelial-mesenchymal transition circPVT1 miR-497-5p TCF3

A Trade-off between Solvation and Collision Activation in Steering Competing E2 and S_(N)2 Dynamics

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Precision Chemistry 2024年第1期2卷 40-48页

作者： Hongyi Wang Xu liu Siwei Zhao Gang Fu wenqing Zhen Li Yang Jiaxu Zhang State Key Laboratory of Urban Water Resource and Environment MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and StorageSchool of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001P.R.China College of Chemistry Liaoning UniversityShenyang 110036P.R.China

The prototypical E2 elimination and SN2 sub-stitution reactions between microsolvated fluoride and ethyl bromide show unexpected dynamic behaviors in mechanistic evolution driven by solvation and collision *** the steric effects,the gas-phase selectivity favors an E2 pathway barely dependent on collision ***,base solvation steers the reaction in an effective way toward substitution at a near-thermal energy,whereas the governing high-energy events retain *** dynamics simulations reproduce exper-imental findings and uncover a crucial solute-solvent coupling in determining such competing ***,collision activation can tune the underlying atomistic dynamics essentially in the reactant entrance channel and cause a mechanism *** features for the ubiquitous competing E2/SN2 dynamics remain quite unknown,providing unique insight into reaction selectivity for complex chemical networks.

关键词： E2/S_(N)2 Competition Atomistic Dynamics Reaction Mechanisms Microsolvation Collision Activation

A machine learning model for diagnosing acute pulmonary embolism and comparison with Wells score,revised Geneva score,and Years algorithm

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Chinese Medical Journal 2024年第6期137卷 676-682页

作者： Linfeng Xi Han Kang Mei Deng wenqing Xu Feiya Xu Qian Gao Wanmu Xie Rongguo Zhang Min liu Zhenguo Zhai Chen Wang Capital Medical University Beijing 100069China National Center for Respiratory Medicine State Key Laboratory of Respiratory Health and MultimorbidityNational Clinical Research Center for Respiratory DiseasesInstitute of Respiratory MedicineChinese Academy of Medical SciencesDepartment of Pulmonary and Critical Care MedicineCenter of Respiratory MedicineChina-Japan Friendship HospitalBeijing 100029China Institute of Advanced Research Infervision Medical Technology Co.Ltd.Beijing 100025China Department of Radiology China-Japan Friendship HospitalBeijing 100029China Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730China Department of Radiology Peking University China-Japan Friendship School of Clinical MedicineBeijing 100191China

Background:Acute pulmonary embolism(APE)is a fatal cardiovascular disease,yet missed diagnosis and misdiagnosis often occur due to non-specific symptoms and signs.A simple,objective technique will help clinicians make a quick and precise *** population studies,machine learning(ML)plays a critical role in characterizing cardiovascular risks,predicting outcomes,and identifying *** work sought to develop an ML model for helping APE diagnosis and compare it against current clinical probability assessment ***:This is a single-center retrospective *** with suspected APE were continuously enrolled and randomly divided into two groups including training and testing sets.A total of 8 ML models,including random forest(RF),Naïve Bayes,decision tree,K-nearest neighbors,logistic regression,multi-layer perceptron,support vector machine,and gradient boosting decision tree were developed based on the training set to diagnose ***,the model with the best diagnostic performance was selected and evaluated against the current clinical assessment strategies,including the Wells score,revised Geneva score,and Years ***,the ML model was internally validated to assess the diagnostic performance using receiver operating characteristic(ROC)***:The ML models were constructed using eight clinical features,including D-dimer,cardiac troponin T(cTNT),arterial oxygen saturation,heart rate,chest pain,lower limb pain,hemoptysis,and chronic heart *** eight ML models,the RF model achieved the best performance with the highest area under the curve(AUC)(AUC=0.774).Compared to the current clinical assessment strategies,the RF model outperformed the Wells score(P=0.030)and was not inferior to any other clinical probability assessment *** AUC of the RF model for diagnosing APE onset in internal validation set was ***:Based on RF algorithm,a novel prediction model was finally constructed for AP

关键词： Acute pulmonary embolism Machine learning Wells score Revised Geneva score Years algorithm

Observation of the nighttime nitrate radical in Hefei, China

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Journal of Environmental Sciences 2008年第1期20卷 45-49页

作者： LI Suwen liu wenqing XIE Pinua LI Ang QIN Min PENG Fumin ZHU Yanwu Key Laboratory of Environmental Optical and Technology Anhui Institute of Optics and Fine MechanicsChinese Academy of Sciences Hefei 230031 China

Observation of nighttime nitrate radical （NO3） was performed by using long path differential optical absorption spectroscopy （LP- DOAS）, on the outskirts of Hefei, China. The time series of NO3 and supporting parameters were simultaneously measured for a week （31 May-7 June 2006）. The results indicated that the average concentration of NO3 was 15.6 pptv with an average lifetimes of 96 s, whereas, NO3 production rates varied from 8×10^2/（cm^3·s） to 2.98×10^7/（cm^3·s）. Furthermore, the calculated N2O5 concentration averaged at 380 pptv. Analysis of data indicated that direct sinks were probably dominating the NO3 loss process during this campaign. The results were compared with other campaigns in the boundary layer.

关键词： nitrate radical nighttime chemistry differential optical absorption spectroscopy sinks

Identification of long-range transport pathways and potential sources of PM2.5 and PM10 in Beijing from 2014 to 2015

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Journal of Environmental Sciences 2017年第6期29卷 214-229页

作者： Deping Li Jianguo liu Jiaoshi Zhang Huaqiao Gui Peng Du Tongzhu Yu Jie Wang Yihuai Lu wenqing liu Yin Cheng Key Laboratory of Environmental Optics and Technology AnhuiInstitute of Optics and Fine MechanicsChinese Academy of SciencesHefei 230031China University of Science and Technology of China Hefei 230026China Center for Excellence in Urban Atmospheric Environment Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China

Trajectory clustering, potential source contribution function （PSCF） and concentration-weighted trajectory （CWT） methods were applied to investigate the transport pathways and identify potential sources of PM2.s and PMIo in different seasons from June 2014 to May 2015 in Beijing. The cluster analyses showed that Beijing was affected by trajectories from the south and southeast in summer and autumn. In winter and spring, Beijing was not only affected by the trajectories from the south and southeast, but was also affected by trajectories from the north and northwest. In addition, the analyses of the pressure profile of backward trajectories showed that backward trajectories, which have important influence on Beijing, were mainly distributed above 970 hPa in summer and autumn and below 950 hPa in spring and winter. This indicates that PM2.s and PMIo were strongly affected by the near surface air masses in summer and autumn and by high altitude air masses in winter and spring. Results of PSCF and CV/T analyses showed that the largest potential source areas were identified in spring, followed by winter and autumn, then summer. In addition, potential source regions of PMIo were similar to those of PM2.5. There were a clear seasonal and spatial variation of the potential source areas of Beijing and the airflow in the horizontal and vertical directions. Therefore, more effective regional emission reduction measures in Beijing＇s surrounding provinces should be implemented to reduce emissions of regional sources in different seasons.

关键词： PM2.5 PM40 Cluster analyses PSCFCWT Beijing

A hydroxyl radical detection system using gas expansion and fast gating laser-induced fluorescence techniques

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Journal of Environmental Sciences 2018年第3期30卷 190-200页

作者： Hao Chen Renzhi Hu Pinhua Xie Xingbiao Xing liuyi Ling Zhiyan Li Fengyang Wang Yihui Wang Jianguo liu wenqing liu Department of Precision Machinery and Precision Instrumentation University of Science and Technology of China Key Laboratory of Environmental Optics and Technology Anhui Institute of Optics and Fine Mechanics Chinese Academy of Sciences CAS Center for Excellence in Regional Atmospheric Environment Institute of Urban Environment Chinese Academy of Sciences Institute of Electric and Information Technology Anhui University of Science and Technology

An OH radical measurement instrument based on Fluorescence Assay by Gas Expansion（FAGE）has been developed in our *** air is introduced into a low-pressure fluorescence cell through a pinhole aperture and irradiated by a dye laser at a high repetition rate of 8.5 k *** OH radical is both excited and detected at 308 nm using A-X（0,0）*** satisfy the high efficiency needs of fluorescence collection and detection,a 4-lens optical system and a self-designed gated photomultiplier（PMT）is used,and gating is actualized by switching the voltage applied on the PMT dynodes.A micro channel photomultiplier（MCP）is also prepared for fluorescence *** the weak signal is accumulated by a photon counter in a specific *** OH radical excitation spectrum range in the wavelength of 307.82–308.2 nm is detected and the excited line for OH detection is determined to be Q1（2）*** calibration of the FAGE system is researched by using simultaneous photolysis of H2O and *** minimum detection limit of the instrument using gated PMT is determined to be 9.4×10~5molecules/cm^3,and the sensitivity is 9.5×10^（-7）cps/（OH·cm^（-3））,with a signal-to-noise ratio of 2 and an integration time of 60 sec,while OH detection limit and the detection sensitivity using MCP is calculated to be 1.6×10~5molecules/cm^3and 2.3×10^（-6）cps/（OH·cm^（-3））.The laboratory OH radical measurement is carried out and results show that the proposed system can be used for atmospheric OH radical measurement.

关键词： OH radical Laser-induced fluorescence Fluorescence assay by gas expansion Photomultiplier Photon counting

Raman光谱及DR技术在可移动文物追溯中的实验研究

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陶瓷学报 2022年第6期43卷 1105-1112页

作者：刘文庆王平南京江宁区文化遗产保护中心江苏南京210000 牛首山文化旅游区管委会文宗科江苏南京210000 南京航空航天大学江苏南京210016 高速载运设施的无损检测监控技术工信部重点实验室江苏南京210016

以江宁博物馆中的纸质类、金属类和陶瓷类文物为检测对象,介绍了包括拉曼(Raman)光谱技术和射线数字(Digital Radiography,DR)技术在内的无损检测技术原理。利用便携式拉曼光谱仪、X射线成像仪等现代科学仪器对可移动文物进行了检测。... 详细信息

以江宁博物馆中的纸质类、金属类和陶瓷类文物为检测对象,介绍了包括拉曼(Raman)光谱技术和射线数字(Digital Radiography,DR)技术在内的无损检测技术原理。利用便携式拉曼光谱仪、X射线成像仪等现代科学仪器对可移动文物进行了检测。检测结果分析表明,拉曼光谱技术检测数据的重复性好,同一点测量数据的自相关达0.99以上,可信度高;不同年代陶瓷文物、书法作品的真迹和仿品的拉曼光谱有明显区别,可以将固定点全谱线的保存作为特征存储。X射线技术能够获得金属和陶瓷类文物的内部细节。以拉曼和X射线为代表的无损检测技术可以为文物特征基因库的建立提供特征元素、为已有档案的文物真伪鉴别提供技术支持。

关键词：文物保护基因库拉曼光谱射线数字

学术语篇中的惊奇与兴趣知识情感：基于FrameNet的研究

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外语与外语教学 2024年第3期 99-110+149页

作者：刘国兵孙文青河南师范大学外国语学院

本研究基于FrameNet考察了惊奇与兴趣两种知识情感在学术语篇中的建构路径。具体而言，本研究通过对语义框架及核心框架元素的分析，探讨不同框架元素在框架系统中的呈现方式，揭示惊奇与兴趣知识情感表达的分布特征。结果显示，惊奇标... 详细信息

本研究基于FrameNet考察了惊奇与兴趣两种知识情感在学术语篇中的建构路径。具体而言，本研究通过对语义框架及核心框架元素的分析，探讨不同框架元素在框架系统中的呈现方式，揭示惊奇与兴趣知识情感表达的分布特征。结果显示，惊奇标记语与兴趣标记语在语言学学科中呈现七种语义框架。此外，在语义框架内部倾向通过现象、属性和关系三种触发类型引起惊奇与兴趣，较少指明触发原因、解决方式与情感体验者。触发程度方面，惊奇情感表述偏好减弱型标记程度，而兴趣情感表述偏好增强型标记程度。上述发现通过知识情感的框架路径揭示了学术知识构建过程和学科共识的合法化过程，为学科话语实践提供新的研究视角。

关键词：知识情感惊奇与兴趣标记语 FrameNet

Modeling of Nucleation and Growth of M_(23)C_6 Carbide in Multi-component Fe-based Alloy

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Journal of Materials Science & Technology 2011年第8期27卷 725-728页

作者： Naqiong Zhu Yanlin He wenqing liu Lin Li Shuigen Huang Jef Vleugels Omer Van der Biest Department of Materials Science and Engineering Shanghai University Shanghai 200072 China Department of Metallurgy and Materials Engineering Katholieke Uniersiteit Leuven Kasteelpark Arenberg 44 B-3001 Heverlee Belgium

Three-dimensional atom probe （3DAP） technique has been used to study the nucleation and growth of M23C6 carbide in a supersaturated multi-component Fe-based alloy aged at 800℃. 3D images indicate that the radius of M23C6 carbide after ageing for 10 min is about 9 nm. Concentration profiles of alloy elements in the carbide are also obtained. Combined with PANDAT and Thermo-Calc software, attempts to model the early stages of precipitation are present. The calculated particle size and composition of M23C6 carbide is in good agreement with 3DAP data.

关键词： Nucleation and growth M23C6 Three-dimensional atom probe (3DAP) Modeling