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Comprehensive analyses of the proteome and ubiquitome revealed mechanism of high temperature accelerating petal abscission in tree peony

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Horticultural Plant Journal 2024年第1期10卷 205-222页

作者： Chunying liu Ziqi liu Yanchao Yuan Yuxi Zhang Yifu Fang Junqiang Chen shupeng Gai Key Laboratory of Plant Biotechnology in Shandong Province College of Life SciencesQingdao Agricultural UniversityQingdaoShandong 266109China Shandong Academy of Forestry JinanShandong 250014China

Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly understood.In this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,respectively.Totally,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were identified.Hydrophilic residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein surface.The differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)level.Additionally,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative RT-PCR.The cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony flowers.Finally,a working model was proposed for the acceleration of flower senescence and abscission by high temperature.In summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony.

关键词： Tree peony Proteome Ubiquitome High temperature Flower senescence

Corporate Power for Poverty Alleviation: Evidence from the Poverty Alleviation Results of Chinese Listed Companies

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China Finance and Economic Review 2022年第2期11卷 46-67页

作者： Liya liu shupeng Zhou Yuanchen Pang Xuehan Guo School of Finance Shanghai University of Finance and Economics Shanghai University of Finance and Economics

Government-led poverty alleviation faces multiple constraints and limitations fighting poverty,and it is urgent to mobilize social forces as many as possible,especially the involvement of corporate forces.To figure out whether corporate participation is effective to poverty alleviation and whether regional differences have an impact on it,this paper probes into results of corporate involvement in poverty alleviation,based on corporate social responsibility report information released by A-share listed companies in 2010-2017.The findings are as follows.(1)Companies in the western region,companies directly contacting consumers and large companies with good business performance are more willing to involve in poverty alleviation.(2)The participation of enterprises in poverty alleviation can increase the per capita income of rural residents,which is even better in underdeveloped areas.This revealed that social forces represented by companies have responded to China’s call of targeted poverty alleviation and fulfilled corporate social responsibilities,which will indeed help consolidate the achievements of poverty alleviation and lift underdeveloped areas out of poverty.From the perspective of corporate involvement in poverty alleviation,this paper extends related research on social forces and another participant in poverty alleviation,and enrich the literature on social benefits brought by companies performing social responsibilities.

关键词： corporate social responsibility anti-poverty corporate involvement in poverty alleviation

30 Years of Climate Change before and after the Impoundment of the Three Gorges Reservoir

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Journal of Earth Science 2023年第4期34卷 1315-1318页

作者： Junqi liu Chen Lin Shan Dong Ruixue Feng Aijun Su Jinghui Cui shupeng Yu Badong National Observation and Research Station of Geohazards China University of GeosciencesWuhan 430074China Three Gorges Research Center for Geo-hazards Ministry of EducationChina University of GeosciencesWuhan 430074China Wuhan Ecological Environment Design and Research Institute Co. LtdWuhan 430050China

The Three Gorges Project, also known as the Yangtze River Three Gorges Water Control Project, is located in Yichang, Hubei Province, China. The dam is one of the largest hydropower stations in the world, with an eleva... 详细信息

关键词： China. Yangtze Gorges

中国叙事艺术的西方批评话语阐释——赛珍珠的中国传统小说写实美学观析论

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江苏大学学报（社会科学版） 2023年第4期25卷 64-73页

作者：刘澍芃南京工业职业技术大学公共基础课部

作为具有双重文化身份的作家、学者,赛珍珠是从跨文化视角审视中国传统小说的。她从艺术哲学的高度谈中国小说写实性时往往借用西方文艺批评的范畴和理路。赛珍珠根据摹仿、再现理论和“镜子说”将中国传统小说比作中国古代社会生活和... 详细信息

作为具有双重文化身份的作家、学者,赛珍珠是从跨文化视角审视中国传统小说的。她从艺术哲学的高度谈中国小说写实性时往往借用西方文艺批评的范畴和理路。赛珍珠根据摹仿、再现理论和“镜子说”将中国传统小说比作中国古代社会生活和历史文化的一面镜子,揭示了中国传统小说在反映社会生活方面具有圣哲典籍无法企及的历史高度和独特价值,并从反映现实与艺术加工关系的维度对中国传统小说的写实美学特征及内涵予以解读。她认为中国传统小说体现了追求真实与艺术加工的统一,同时也带有自然主义冷静、客观、实录性的书写及观察、记载人性在具体环境中的表现等特征,不是仅停留在“像生活”的层面,而是创造出一种“是生活”的感觉,与自然主义“实验小说”的原则相符。

关键词：赛珍珠中国小说跨文化艺术哲学写实美学实验小说

Dual functions of PsmiR172b-PsTOE3 module in dormancy release and flowering in tree peony (Paeonia suffruticosa)

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Horticulture Research 2023年第4期10卷 174-186页

作者： Yuxi Zhang Linqiang Gao Yanyan Wang Demei Niu Yanchao Yuan Chunying liu Xinmei Zhan shupeng Gai College of Life Sciences Qingdao Agricultural UniversityQingdao266109China University Key Laboratory of Plant Biotechnology in Shandong Province Qingdao266109China

MicroRNAs(miRNAs)are non-coding RNAs that interact with target genes and are involved in many physiological processes in plants.miR172-AP2 mainly plays a role in the regulation of f lowering time and floral organ differentiation.Bud dormancy release is necessary for forcing culture of tree peony in winter,but the mechanism of dormancy regulation is unclear.In this study,we found that a miR172 family member,PsmiR172b,was downregulated during chilling-induced bud dormancy release in tree peony,exhibiting a trend opposite to that of PsTOE3.RNA ligase-mediated(RLM)5-RACE(rapid amplification of cDNA ends)confirmed that miR172b targeted PsTOE3,and the cleavage site was between bases 12(T)and 13(C)within the complementary site to miR172b.The functions of miR172b and PsTOE3 were detected by virus-induced gene silencing(VIGS)and their overexpression in tree peony buds.PsmiR172b negatively regulated bud dormancy release,but PsTOE3 promoted bud dormancy release,and the genes associated with bud dormancy release,including PsEBB1,PsEBB3,PsCYCD,and PsBG6,were upregulated.Further analysis indicated that PsTOE3 directly regulated PsEBB1 by binding to its promoter,and the specific binding site was a C-repeat(ACCGAC).Ectopic expression in Arabidopsis revealed that the PsmiR172b-PsTOE3 module displayed conservative function in regulating f lowering.In conclusion,our results provided a novel insight into the functions of PsmiR172-PsTOE3 and possible molecular mechanism underlying bud dormancy release in tree peony.

关键词： release functions bases

Identification of portal vein tumor thrombus with an independent clonal origin in hepatocellular carcinoma via multi-omics data analysis

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Cancer Biology & Medicine 2019年第1期16卷 147-156页

作者： shupeng liu Zaixin Zhou Yin Jia Jie Xue Zhiyong liu Kai Cheng Shuqun Cheng Shanrong liu Clinical Research Center Changhai Hospital Second Military Medical University Department of Laboratory Diagnostics Changhai Hospital Second Military Medical University Department of Hepatic Surgery VI Eastern Hepatobiliary Surgery Hospital Second Military Medical University

Objective: Multiple mechanisms underlying the development of portal vein tumor thrombus(PVTT) in hepatocellular carcinoma(HCC) have been reported recently. However, the origins of PVTT remain unknown. Increasing multi-omics data on PVTTs in HCCs have made it possible to investigate whether PVTTs originate from the corresponding primary tumors(Ts).Methods: The clonal relationship between PVTTs and their corresponding primary Ts was investigated using datasets deposited in public databases. One DNA copy number variations dataset and three gene expression datasets were downloaded for the analyses.Clonality analysis was performed to investigate the clonal relationship between PVTTs and Ts from an individual patient.Differential gene expression analysis was applied to investigate the gene expression profiles of PVTTs and Ts.Results: One out of 19 PVTTs had no clonal relationship with its corresponding T, whereas the others did. The PVTTs with independent clonal origin showed different gene expression and enrichment in biological processes from the primary Ts. Based on the unique gene expression profiles, a gene signature including 24 genes was used to identify pairs of PVTTs and primary Ts without any clonal relationship. Validation in three datasets showed that these types of pairs of PVTTs and Ts can be identified by the 24-gene signature.Conclusions: Our findings show a direct evidence for PVTT origin and consolidate the heterogeneity of PVTTs observed in clinic.The results suggest that PVTT investigation at a molecular level is clinically necessary for diagnosis and treatment.

关键词： Hepatocellular carcinoma portal vein tumor thrombus clonal origin copy number variation bioinformatics

Numerical Simulations of Hydrodynamics of Nematic Liquid Crystals:Effects of Kinematic Transports

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Communications in Computational Physics 2011年第4期9卷 974-993页

作者： shupeng Zhang Chun liu Hui Zhang School of Mathematical Sciences Beijing Normal UniversityBeijing 100875China Department of Mathematics Pennsylvania State UniversityUniversity ParkPA 18601USA.

In this paper,we investigate the effects of kinematic transports on the nematic liquid crystal system numerically and theoretically.The model we used is a"1+2"elastic continuum model simplified from the Ericksen-Leslie system.The numerical experiments are carried out by using a Legendre-Galerkin spectral method which can preserve the energy law in the discrete form.Based on this highly accurate numerical approach we find some interesting and important relationships between the kinematic transports and the characteristics of the flow.We make some analysis to explain these results.Several significant scaling properties are also verified by our simulations.

关键词： Nematic liquid crystals kinematic transports Legendre-Galerkin spectral method tumbling flow-aligning

Carbon-coated magnetic particles increase tissue temperatures after laser irradiation

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Journal of Innovative Optical Health Sciences 2015年第5期8卷 50-55页

作者： shupeng liu Na Chen Fufei Pang Zhengyi Chen Tingyun Wang Key Laboratory of Specialty Fiber Optics and Optical Access Networks Shanghai University 149 Yanchang Road Shanghai 200072P.R.China Institute of Biomedical Engineering Shanghai University 149 Yanchang RoadShanghai 200072P.R.China

Purpose:This work focused on the investigation the hyperthermia performance of the carboncoated magnetic particles(CCMPs)in laser-induced hyperthermia.Materials and methods:We prepared CCMPs using the organic carbonization method,and then characterized them with transmission electron microscopy(TEM),ultraviolet-visible(UV-Vis)spectrophotometry,vibrating sample magnetometer(VSM)and X-ray di®raction(XRD).In order to evaluate their performance in hyperthermia,the CCMPs were tested in laser-induced thermal therapy(LITT)experiments,in which we employed a fully distributedfiber Bragg grating(FBG)sensor to profile the tissue's dynamic temperature change under laser irradiation in real time.Results:The sizes of prepared CCMPs were about several micrometers,and the LITT results show that the tissue injected with the CCMPs absorbed more laser energy,and its temperature increased faster than the contrast tissue without CCMPs.Conclusions:The CCMPs may be of great help in hyperthermia applications.

关键词： Carbon-coated magnetic particles hyperthermia laser

PsRGL1 negatively regulates chilling- and gibberellin-induced dormancy release by PsF-box1-mediated targeting for proteolytic degradation in tree peony

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Horticulture Research 2023年第5期10卷 49-60页

作者： Linqiang Gao Demei Niu Tianyu Chi Yanchao Yuan Chunying liu shupeng Gai Yuxi Zhang College of Life Sciences Qingdao Agricultural UniversityQingdao 266109China University Key Laboratory of Plant Biotechnology in Shandong Province Qingdao 266109China

Tree peony bud endodormancy is a common survival strategy similar to many perennial woody plants in winter,and the activation of the GA signaling pathway is the key to breaking endodormancy.GA signal transduction is involved in many physiological processes.Although the GA-GID1-DELLA regulatory module is conserved in many plants,it has a set of specific components that add complexity to the GA response mechanism.DELLA proteins are key switches in GA signaling.Therefore,there is an urgent need to identify the key DELLA proteins involved in tree peony bud dormancy release.In this study,the prolonged chilling increased the content of endogenously active gibberellins.PsRGL1 among three DELLA proteins was significantly downregulated during chilling-and exogenous GA3-induced bud dormancy release by cell-free degradation assay,and a high level of polyubiquitination was detected.Silencing PsRGL1 accelerated bud dormancy release by increasing the expression of the genes associated with dormancy release,including PsCYCD,PsEBB1,PsEBB3,PsBG6,and PsBG9.Three F-box protein family members responded to chilling and GA3 treatments,resulting in PsF-box1 induction.Yeast two-hybrid and BiFC assays indicated that only PsF-box1 could bind to PsRGL1,and the binding site was in the C-terminal domain.PsF-box1 overexpression promoted dormancy release and upregulated the expression of the dormancy-related genes.In addition,yeast two-hybrid and pull-down assays showed that PsF-box1 also interacted with PsSKP1 to form an E3 ubiquitin ligase.These findings enriched the molecular mechanism of the GA signaling pathway during dormancy release,and enhanced the understanding of tree peony bud endodormancy.

关键词： release tree breaking