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烟盲蝽对不同虫态烟粉虱的捕食作用及捕食偏好性

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环境昆虫学报 2024年第3期46卷 737-743页

作者：曹璐侯启华刘树生刘银泉浙江大学昆虫科学研究所浙江省作物病虫生物学重点实验室农业农村部作物病虫分子生物学重点实验室杭州310058

杂食性昆虫烟盲蝽Nesidiocoris tenuis是烟粉虱Bemisia tabaci的重要天敌之一。为探索烟盲蝽对不同虫态烟粉虱的捕食作用及捕食偏好性,在室内进行了非选择性和选择性试验。结果表明,在非选择性试验中,烟盲蝽雌成虫、雄成虫以及5龄若虫... 详细信息

杂食性昆虫烟盲蝽Nesidiocoris tenuis是烟粉虱Bemisia tabaci的重要天敌之一。为探索烟盲蝽对不同虫态烟粉虱的捕食作用及捕食偏好性,在室内进行了非选择性和选择性试验。结果表明,在非选择性试验中,烟盲蝽雌成虫、雄成虫以及5龄若虫可以捕食烟粉虱成虫和各龄若虫,但极少捕食卵。其中,烟盲蝽雌成虫、雄成虫及5龄若虫对烟粉虱1龄若虫的捕食量最大,分别为134.2头、101.3头和88.1头,其次是2~3龄若虫,捕食量分别为94.1头、64.2头和53.4头。而在选择性试验中烟盲蝽雄虫更偏好捕食烟粉虱的2~3龄若虫,其次是1龄若虫、4龄若虫和成虫。该结果对了解烟盲蝽控制烟粉虱的作用以及如何对烟盲蝽和其他天敌组合应用提供了参考信息。

关键词：烟盲蝽烟粉虱捕食偏好性 Cain指数生物防治

Tomato yellow leaf curl virus infection of tomato does not affect the performance of the Q and ZHJ2 biotypes of the viral vector Bemisia tabaci

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Insect Science 2011年第1期18卷 40-49页

作者： Meng Li Jian liu shu-sheng liu Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Insect Sciences Zhejiang UniversityHangzhou China

To better understand the etiology of begomovirus epidemics in regions under invasion we need to know how indigenous and invasive whitefly vectors respond to virus infection. We investigated both direct and indirect effects of infection with Tomato yellow leaf curl virus （TYLCV） on the performance of the invasive Q biotype and the indigenous Asian ZHJ2 biotype of whitefly Bemisia tabaci. The Q biotype performed better than the ZHJ2 biotype on either uninfected or virus-infected tomato plants. However, virus-infection of host plants did not, or only marginally affected, the performance of either biotype of whiteflies in terms of fecundity, longevity, survival, development and population increase. Likewise, association of the vectors with TYLCV did not affect fecundity and longevity of the Q or ZHJ2 biotypes on cotton, a non-host of TYLCV. These results indicate that the alien Q biotype whitefly, but not the indigenous ZHJ2 biotype, is likely to become the major vector of TYLCV in the field and facilitate virus epidemics.

关键词： Bemisia tabaci performance tomato Tomato yellow leaf curl virus virusinfection

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孤本宋刻钱杲之《离骚集传》递藏轨迹考述

三峡大学学报（人文社会科学版） 2024年第2期46卷 29-36页

作者：刘树胜张国清金陵科技学院人文学院江苏南京211100 金陵科技学院外国语学院江苏南京211100

国图藏钱杲之《离骚集传》,根据其题跋、题识、题签与藏书印,结合对与之相关的历代书目文献的考察,一条完整的递藏链就清晰地凸现出来了:孤本宋刻钱杲之《离骚集传》首藏于江阴朱承爵“存与堂”,由其后人朱元孺递藏;又藏于常熟毛晋“汲... 详细信息

国图藏钱杲之《离骚集传》,根据其题跋、题识、题签与藏书印,结合对与之相关的历代书目文献的考察,一条完整的递藏链就清晰地凸现出来了:孤本宋刻钱杲之《离骚集传》首藏于江阴朱承爵“存与堂”,由其后人朱元孺递藏;又藏于常熟毛晋“汲古阁”,由其子毛褒、毛表递藏;继藏于丰润曹寅“楝亭”;继藏于桐乡金德舆“桐华馆”;继藏于平江黄丕烈“士礼居”;继藏于长洲汪士钟“艺芸书舍”,由其后人汪宪奎、汪振勋递藏;继藏于常熟瞿绍基“铁琴铜剑楼”,由其后人瞿镛、瞿润、瞿秉渊、瞿启科、瞿启甲、瞿济苍、瞿旭初、瞿凤起祖孙五代递藏(瞿启甲掌握期间,武进费念慈和常熟邵松年均曾向其借以影抄录副),1950年由瞿济苍、瞿旭初、瞿凤起兄弟将其捐献于中央人民政府,今藏于国家图书馆。同时我们仍然可以看到作者对楚辞研究的细心、认真与贡献,这也充分证明了此书确为宋刻孤本而非影抄本。

关键词：孤本宋刻《离骚集传》藏书印题跋题识题签递藏链

Species Concepts as Applied to the Whitefly Bemisia tabaci Systematics:How Many Species Are There?

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Journal of Integrative Agriculture 2012年第2期11卷 176-186页

作者： liu shu-sheng John Colvin Paul J De Barro Key Laboratory of Agricultural Entomology Ministry of Agriculture~Institute of lnsect Sciences Zhejiang University Hangzhou 310058 P.R. China Natural Resources Institute University of Greenwich Kent ME4 4TB United Kingdom CSIRO Ecosystem Sciences Brisbane QLD 4001 Australia

The worldwide distribution and extensive genetic diversity of the whitefly,Bemisia tabaci,has long been ***,the levels of separation within *** and the nomenclature of the various genetic groups have been a subject of *** phylogenetic analyses indicate that *** is a complex composed of 28 morphologically indistinguishable *** this article,we first review the debate and difficulties associated with ***＇s taxonomy and systematics,and argue for the need to apply the biological species concept in order to elucidate ***＇s *** summarize the accumulated genetic and behavioural data on reproductive incompatibilities evident amongst phylogenetic mtCOI groups of *** studies have been conducted with 14 of the 28 putative species covering 54 reciprocal inter-species pairs,and observations on mating behaviour have been conducted for seven species *** from both crossing trials and behavioural observations indicate a consistent pattern of reproductive isolation among the putative *** then discuss the technical and conceptual complexities associated with crossing experiments and behavioural observations designed to reveal reproductive ***,we elaborate on a strategy for further clarifying the pattern of reproductive isolation between *** groups and propose future research directions on the systematics of this complex.

关键词： whitefly systematics biological species cryptic species phylogenetic species reproductive isolation mating behavior taxonomy

Species Exclusion Between an Invasive and an Indigenous Whitefly on Host Plants with Differential Levels of Suitability

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Journal of Integrative Agriculture 2012年第2期11卷 215-224页

作者： LUAN Jun-bo XU Jing LIN Ke-ke Myron P Zalucki liu shu-sheng Key Laboratory of Agricultural Entomology Minstry of Agriculture~Institute of lnsect Sciences Zhejiang University Hangzhou 310029 P.R.China School of Biological Sciences The University of Queensland Queensland 4072 Australia

The whitefly Bemisia tabaci has risen to international prominence since the 1980s due to the rapid spread around the globe by the two species B and Q within this species *** invasion of B has often been associated with the displacement of indigenous *** the genetic structure of *** is diverse,more case studies of the competitive relationships between B and indigenous species of the whitefly species complex will help to understand further the mechanisms underlying the invasion of *** examined the competitive interactions between B and ZHJ2,a widely distributed indigenous whitefly in Asia,on host plants with differential levels of suitability to the two species in the laboratory,and also tested the effect of insecticide application on the competitive *** species of plants were tested including cotton,a plant showing similar levels of suitability to both species,squash,a plant showing higher suitability to B than to ZHJ2,and kidney bean,a plant showing higher suitability to ZHJ2 than to *** the case of no insecticide application,B displaced ZHJ2 on cotton,squash,and kidney bean by the 6th,3rd and 10th generation,*** the application of imidacloprid,the displacement of ZHJ2 by B on cotton occurred by the 5th *** the displacement progressed,the proportion of B females increased,and the proportion of ZHJ2 females decreased on cotton and *** contrast,on kidney bean the proportion of B females remained unchanged while that of ZHJ2 *** results show the strong capacity of the invasive B to displace ZHJ2,and indicate that host plants with differential levels of suitability to the two species may affect the speed but not the trend of displacement and insecticide application may accelerate the process of displacement.

关键词： Bemisia tabaci competitive displacement insecticide invasive species

p38 MAPK is a Component of the Signal Transduction Pathway Triggering Cold Stress Response in the MED Cryptic Species of Bemisia tabaci

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Journal of Integrative Agriculture 2012年第2期11卷 303-311页

作者： LI Fang-fang XIA Jun LI Jun-min liu shu-sheng WANG Xiao-wei Key Laboratory of Agricultural Entomology Ministry of Agriculture/Institute of Insect SciencesZhejiang UniversityHangzhou 310029P.R.China

Cold stress responses help insects to survive under low temperatures that would be lethal *** phenomenon might contribute to the invasion of some Bemisia tabaci cryptic species from subtropical areas to temperate ***,the molecular mechanisms regulating cold stress responses in whitefly are yet ***-activated protein kinases（MAPKs）which including p38,ERK,and JNK,are well known for their roles in regulating metabolic responses to cold stress in many *** this study,we explored the possible roles of the MAPKs in response to low temperature stresses in the Mediterranean cryptic species（the Q-biotype）of the *** species ***,we cloned the p38 and ERK genes from the whitefly cDNA ***,we analyzed the activation of MAPKs during cold stress in the Mediterranean cryptic species by *** cold stress,the level of phospho-p38 increased but no significant change was observed in the phosphorylation of ERK and JNK,thus suggesting that the p38 might be responsible for the defense response to low temperature ***,we demonstrated that：i）3 min chilling at 0°C was sufficient for the activation of p38 MAPK pathway in this whitefly;and ii）the amount of phosphorylated p38 increased significantly in the first 20 min of chilling,reversed by 60 min,and then returned to the original level by 120 *** together,our results suggest that the p38 pathway is important during response to low temperature stress in the Mediterranean cryptic species of the *** species complex.

关键词： Bemisia tabaci cold stress MAPK p38 phosphorylation signal transduction

Effect of Exogenous MJA Treatment of Tea Plants on the Growth of Geometrid Larvae

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Agricultural Sciences in China 2005年第9期4卷 673-679页

作者： GUI Lian-you CHEN Zong-mao liu shu-sheng Institute of Applied Entomology Zhejiang University Hangzhou 310029 P.R.China Key Laboratory of Tea Chemical Engineering Ministry of Agriculture/Tea Research Institute Chinese Academy of Agricultural Sciences Hangzhou 310008 P.R China Agricultural College Yangtze University Hubei 434025 P.R. China

The effect of tea plant Camellia sinensis induced by exogenous methyl jasmonate （MJA） on lipoxygenase （LOX）, polyphenol oxidase （PPO） and proteinase inhibitor （PI） activity in the leaves of tea plants, as well as the growth and midgut proteinase activity of the geometrid Ectropis obliqua larvae were studied. MJA significantly induced LOX, PPO and PI activity in leaves of tea plants. When geometrid larvae have fed on leaves of tea plants treated with MJA, the activities of the high alkaline trypsin-like enzyme and chymotrypsin-like enzyme in their midgut were significantly inhibited, but the activities of the low alkaline trypsin-like enzyme in their midgut were unaffected, leading to imbalance between different types of proteinase activity in the midgut of the larvae and in turn, the growth were inhibited. These chains of response may be an important mechanism of the direct resistance induced by MJA-treatment of tea plant on geometrid larvae.

关键词： Methyl jasmonate Proteinase inhibitors Midgut proteinase Ectropis obliqua Camellia sinensis

Enhancing cellular behavior in repaired tissue via silk fibroin-integrated triboelectric nanogenerators

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Microsystems & Nanoengineering 2024年第3期10卷 137-147页

作者： Zhelin Li shuxing Xu Zijie Xu sheng shu Guanlin liu Jianda Zhou Ding Lin Wei Tang Changsha Aier Eye Hospital Aier School of OphthalmologyCentral South UniversityChangshaHunanChina The Xiangya Hospital Central South UniversityChangshaHunanChina Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 101400China Center on Nanoenergy Research School of Physical Science&TechnologyGuangxi UniversityNanning 530004China School of Nanoscience and Technology University of Chinese Academy of SciencesBeijing 100049China Department of Plastic Surgery the Third Xiangya HospitalChangshaHunanChina

Triboelectric nanogenerators(TENGs)have emerged as a promising approach for generating electricity and providing electrical stimuli in medical electronic *** their potential benefits,the clinical implementation of TENGs faces challenges such as skin compliance and a lack of comprehensive assessment regarding their biosafety and ***,further research is imperative to overcome these limitations and unlock the full potential of TENGs in various biomedical *** this study,we present a flexible silk fibroin-based triboelectric nanogenerator(SFB-TENG)that features an on-skin substrate and is characterized by excellent skin compliance and air/water *** range of electrical output generated by the SFB-TENG was shown to facilitate the migration and proliferation of Hy926,NIH-3T3 and RSC96 ***,apoptosis of fibroblast NIH-3T3 cells was observed when the output voltage increased to more than 20 V at a frequency of 2 *** addition,the moderate electrical stimulation provided by the SFB-TENG promoted the cell proliferation cycle in Hy926 *** research highlights the efficacy of a TENG system featuring a flexible and skin-friendly design,as well as its safe operating conditions for use in biomedical *** findings position TENGs as highly promising candidates for practical applications in the field of tissue regeneration.

关键词： overcome spite generating

Fe/S oxidation-coupled arsenic speciation transformation mediated by AMD enrichment culture under different pH conditions

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Journal of Environmental Sciences 2024年第3期137卷 681-700页

作者： Yu-hang Zhou Wei-xi Huang Zhen-yuan Nie Hong-chang liu Yue liu Can Wang Jin-lan Xia Wen-sheng shu Key Lab of Biometallurgy of Ministry of Education of China School of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China School of Life Sciences South China Normal UniversityGuangzhou 510631China

Arsenic(As)speciation transformation in acid mine drainage(AMD)is comprehensively affected by biological and abiotic factors,such as microbially mediated Fe/S redox reactions and changes in environmental conditions(pH and oxidation-reduction potential).However,their combined impacts on arsenic speciation transformation remain poorly ***,we explored arsenic transformation and immobilization during pyrite dissolution mediated by AMD enrichment culture under different acidic pH *** results for incubation and mineralogical transformation of solid residues show that in the presence of AMD enrichment culture,pH 2.0,2.5,and 3.0 are more conducive to the formation of jarosites and ferric arsenate,which could immobilize high quantities of dissolved arsenic by adsorption and *** pH conditions significantly affect the initial adsorption of microbial cells to the minerals and the evolution of microbial community structure,further infuencing the biodissolution of pyrite and the release and oxidation process of Fe/*** results of Fe/S/As speciation transformation of the solid residues show that the transformation of Fe,S,and As in solution is mainly regulated by pH and potential values,which imposed significantly different effects on the formation of secondary minerals and thus arsenic oxidation and *** above results indicated that arsenic transformation is closely related to the Fe/S oxidation associated with pyrite bio-oxidation,and this correlation is critically regulated by the pH conditions of the system.

关键词： Acid mine drainage(AMD) pH conditions Fe/S/As speciation transformation Microbial community structure