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Interacted Effect of Arbuscular Mycorrhizal Fungi and Polyamines on Root System Architecture of Citrus Seedlings

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Journal of Integrative Agriculture 2012年第10期11卷 1675-1681页

作者： WU Qiang-sheng ZOU Ying-ning liu chun-yan LU Ting College of Horticulture and Gardening Yangtze University

Either arbuscular mycorrhizal fungi （AMF） or polyamines （PAs） may change root system architecture （RSA） of plants, whereas the interaction of AMF and PAs on RSA remains unclear. In the present study, we studied the interaction between AMF （Paraglomus occultum） and exogenous PAs, including putrescine （Put）, spermidine （Spd） and spermine （Spin） on mycorrhizal development of different parts of root system, plant growth, RSA and carbohydrate concentrations of 6-m-old citrus （Citrus tangerine Hort. ex Tanaka） seedlings. After 14 wk of PAs application, PA-treated mycorrhizal seedlings exhibited better mycorrhizal colonization and numbers of vesicles, arbuscules, and entry points, and the best mycorrhizal status of taproot, first-, second-, and third-order lateral roots was respectively found in mycorrhizal seedlings supplied with Put, Spd and Spm, suggesting that PAs might act as a regulated factor of mycorrhizal development through transformation of root sucrose more into glucose for sustaining mycorrhizal development. AMF usually notably increases RSA traits （taproot length, total length, average diameter, projected area, surface area, volume, and number of first-, second-, and third-order lateral roots） of only PA-treated seedlings. Among the three PA species, greater positive effects on RSA change and plant biomass increment of the seedlings generally rank as Spd〉Spm〉Put, irrespective of whether or not AMF colonization. PAs significantly changed the RSA traits in mycorrhizal but not in non-mycorrhizal seedlings. It suggests that the application of PAs （especially Spd） to AMF plants would optimize RSA of citrus seedlings, thus increasing plant growth （shoot and root dry weight）.

关键词： citrus lateral root mycorrhiza putrescine root system architecture spermidine spermine

Leucosceptroid B from glandular trichomes of Leucosceptrum canum reduces fat accumulation in Caenorhabditis elegans through suppressing unsaturated fatty acid biosynthesis

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Chinese Journal of Natural Medicines 2019年第12期17卷 892-899页

作者： LING Yi TENG Lin-Lin HUA Juan LI De-Sen LUO Shi-Hong liu yan-chun liu yan LI Sheng-Hong State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Yunnan Key Laboratory of Natural Medicinal Chemistry Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Obesity that is highly associated with numerous metabolic diseases has become a global health issue *** sesterterpenoids are an important group of natural products with great potential;thus,their bioactivities deserve extensive ***-seq analysis indicated that leucosceptroid B,a sesterterpenoid previously discovered from the glandular trichomes of Leucosceptrum canum,significantly regulated the expression of 10 genes involved in lipid metabolism in Caenorhabditis ***,leucosceptroid B was found to reduce fat storage,and downregulate the expression of two stearoyl-CoA desaturase(SCD)genes fat-6 and fat-7,and a fatty acid elongase gene elo-2 in wild-type *** addition,leucosceptroid B significantly decreased fat accumulation in both fat-6 and fat-7 mutant worms but did not affect the fat storage of fat-6;fat-7 double *** findings indicated that leucosceptroid B reduced fat storage depending on the downregulated expression of fat-6,fat-7 and elo-2 and thereby inhibiting the biosynthesis of the corresponding unsaturated fatty *** findings provide new insights into the development and utilization of plant sesterterpenoids as potential antilipemic agents.

关键词： Sesterterpenoid Leucosceptroid B Leucosceptrum canum Caenorhabditis elegans Fat accumulation Fatty acid biosynthesis Antilipemic agent

Simulation study on hyperchaos analysis of reforming system based on single-ring erbium-doped fiber laser

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The Journal of China Universities of Posts and Telecommunications 2013年第5期20卷 117-121页

作者： PU Xin TIAN Xiao-jian ZHAI Hong-yi QIAO Liang liu chun-yan CUI yan-qun College of Electronic Science and Engineering Jilin University College of Optical and Electronical Information Changchun University of Science and Technology

A reforming dynamic system based on the single-ring erbium-doped fiber laser is proposed in this paper. The reforming system has larger Lyapunov exponent and better pseudorandom characteristics according to the simula... 详细信息

关键词： erbium-doped fiber laser hyperchaos reforming system

Establishing dynamic thresholds for potato nitrogen status diagnosis with the SPAD chlorophyll meter

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Journal of Integrative Agriculture 2015年第1期14卷 190-195页

作者： ZHENG Hong-li liu yan-chun QIN Yong-lin CHEN yang FAN Ming-shou College of Agronomy Inner Mongolia Agricultural University Center of Biotechnology Chifeng Academy of Agricultural and Animal Husbandry Sciences College of Ecology and Environment Science Inner Mongolia Agricultural University

The hand-held soil plant analysis development （SPAD） chlorophyll meter nitrogen status of the potato and guiding fertilization recommendations N recommendation, it is critical to establish the threshold SPAD value has proved to be a promising tool in evaluating the n the process of N evaluation of potato plants and （SPAD reading）, below which nitrogen supplement is required. And taking convenient using into account, the threshold needs to be dynamic throughout the potato growing season so that the users can test their potato plants and make fertilization decision at any growing time of potato. To complete this goal, field experiments with different nitrogen supply levels were conducted in different sites in northern China from 2009 to 2011. The results showed that threshold SPAD values decrease as the growing season progresses for all cultivars and planting sites. By statistical analysis, the threshold regression models were established respectively as： y=-0.003χ2-0.0507χ＋58.213 （y, threshold SPAD value; χ, days after emergence） for the potato cultivar Kexin 1, and y=-0.003χ2＋0.017χ＋52.489 （y, threshold SPAD value; χ, days after emergence） for the cultivar Shepody, from which, the threshold SPAD value at any day after emergence can be calculated.

关键词： diagnosis dynamic threshold N status potato SPAD meter

Estimation of the carbon storage of forest vegetation and carbon emission from forest fires in Heilongjiang Province,China

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Journal of Forestry Research 2007年第1期18卷 17-22页

作者： HU Hai-qing liu Yuan-chun JIAO yan College of Forestry Northeast Forestry University Harbin 150040 P. R. China

The forest resource of Heilongjiang province has important position in china. On the basis of the six times of national forest inventory data （1973-1976, 1977-1981, 1985-1988, 1989-1993, 1994-1998, 1999-2003） surveyed by the Forestry Ministry of P. R. China from 1973 to 2003, the carbon storage of forests in Heilongjiang Province are estimated by using the method of linear relationship of each tree species between biomass and volume. The results show that the carbon storage of Heilongjiang forests in the six periods （1973-1976, 1977-1981, 1985-1988, 1989-1993, 1994-1998, 1999-2003） are 7.164×10^8 t, 4.871×10^8 t, 5.094×10^8 t, 5.292×10^8 t, 5.594×10^8 t and 5.410×10^8 t, respectively., which showed a trend of decreasing in early time and then increasing. It indicated that Heilongjiang forests play an important role as a sink of atmospheric carbon dioxide during past 30 years. Based on the data of forest fires from 1980 to 1999 and ground biomass estimation for some forest types in Heilongjiang Province, it is estimated that the amount of mean annual consumed biomass of forests is 391758.65t-522344.95t, accounting for 6.4%-8.4% of total national consummation from forest fires, and the amount of carbon emission is 176 291.39t-235 055.23t, about 8% of total national emission from forest fires. The emission of CO2, CO, CH4 and NMHC from forest fires in Heilongjiang Province are estimated at 581761.6-775682.25 t, 34892.275-46523.04 t, 14091.11-18788.15 t and 6500-9000 t, respectively, every year.

关键词： Forest vegetation Carbon storage Forest fire Biomass Carbon emission

The Enlightenment of Metaphors in Titles on English Writing Teaching

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Sino-US English Teaching 2011年第11期8卷 706-712页

作者： liu yan-chun Harbin Engineering University Harbin China

Based on non-English majors＇ textbooks, this paper discusses the functions of metaphors in titles of texts and places a major emphasis on enlightenment of metaphors in titles on English writing teaching. And through analyzing metaphors in titles, it is suggested that in daily writing teaching, teachers should bear the cultivation of students＇ metaphorical thinking from analyzing titles in mind; teachers also design some writing tasks with metaphors in titles and select textbooks with metaphor-rich texts

关键词： title metaphor English writing teaching

Anaphora＇s Creating Irony by the Aid of Context

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Sino-US English Teaching 2015年第9期12卷 647-653页

作者： liu yan-chun Harbin Engineering University Harbin China

The core function of anaphora is to serve as a substitute for a certain linguistic component in texts, but it actually has other missions such as creating the rhetorical effect--irony. However, anaphora alone cannot contribute to ironic effect, and only by resorting to other resources like contextual information, can anaphora generate irony. Therefore, Yus＇ s classification of contextual sources is employed to analyze with examples how anaphora creates irony by the aid of context. Ultimately, the conclusion has been drawn that anaphora is able to produce irony with the help of six groups of contextual information classified by Yus except the category--speaker＇s nonverbal behavior and anapbora sometimes has to interact with several groups of contextual information in order to create irony.

关键词： anaphora irony contextual information

An Integrated QTL Map of Fungal Disease Resistance in Soybean (Glycine max L. Merr):A Method of Meta-Analysis for Mining R Genes

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Agricultural Sciences in China 2010年第2期9卷 223-232页

作者： WANG Jia-lin liu chun-yan WANG Jing QI Zhao-ming LI Hui HU Guo-hua CHEN Qing-shan College of Agriculture Northeast Agricultural University Harbin 150030 P.R.China Land Reclamation Research ＆ Breeding Centre of Heilongjiang Harbin 150090 P.R.China The National Research Center of Soybean Engineering and Technology Harbin 150050 P.R.China College of Science Northeast Agricultural University Harbin 150030 P.R.China

Diseases caused by fungal pathogens account for approximately 50% of all soybean disease losses around the world. Conflicting results of fungal disease resistance QTLs from different populations often occurred. The objectives of this study were to：（i） evaluate evidence for reported fungal disease resistance QTLs associations in soybean and （ii） extract relatively reliable and useful information from the ＂real＂ QTLs and mine putative genes in soybean. An integrated map of fungal disease resistance QTLs in soybean was established with soymap 2 published in 2004 as a reference map. QTLs of fungal disease resistance developed from each of separate populations in recent 10 years were integrated into a combinative map for gene cloning and marker assisted selection in soybean. 107 QTLs from different maps were integrated and projected to the reference map with the software BioMercator 2.1. A method of meta-analysis was used to narrow down the confidence interval, and 23 ＂real＂ QTLs and their corresponding markers were obtained from 12 linkage groups （LG）, respectively. Two published R genes were found in these ＂real＂ QTLs intervals. Sequences in the ＂real＂ QTLs intervals were predicted by GENSCAN, and these predicted genes were annotated in Goblet. 228 resistance gene analogs （RGAs） in 12 different terms were mined. The results will lay the foundation for a bioinformatics platform combining abundant QTLs, and offer the basis for marker assisted selection and gene cloning in soybean.

关键词： soybean fungal disease QTL meta-analysis resistance gene analogs

Genetic Analysis and Molecular Mapping of Two SMV-Resistance Traits in Soybean:Adult-Plant Resistance and Resistance to Seed Coat Mottling

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Agricultural Sciences in China 2010年第1期9卷 11-18页

作者： WANG Kun LI Wen-fu ZHANG Lei liu chun-yan ZHU Xiao-shuang CHEN Qing-shan HU Guo-hua Agriculture Academy Northeast Agricultural University/Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province/ National Research Center of Soybean Engineering and Technology/Soil Fertility and Environmental Resources Institute Heilongjiang Academy of Agricultural Sciences Harbin 150090 P.R.China

Soybean mosaic virus （SMV） could lead to adult-plant system diseases, and cause mottling of soybean seeds. Genetic analysis and molecular mapping were conducted using an F2 population and derived F3 families from two crosses of Dongnong 3C624 （susceptible）x Dongnong 8143 （resistant） and Dongnong 3C628 （susceptible）× Tie 6915 （resistant）. Simple sequence repeat （SSR） markers with bulked segregation analysis （BSA） were used to conduct genetic mapping of the resistance to SMV1 in the segregating populations. The results indicated that resistance to SMV1 in adultplants and the resistance to seed coat mottling in Dongnong 8143 and Tie 6915 was separately controlled by one single dominant gene. The two dominant genes were identified to be linked on the MLG F by Mendel＇s genetics and SSR genetic mapping. The order and distance of markers DPRSMV1 and DSRSMV1 were Sat 229-6.9 cM-DSRSMV1-4.6 cM-Sat_317-3.6 cM-DPRSMV1-5.2 cM-Satt335. The order and distance of markers TPRSMV1 and TSRSMV1 was Satt160-16.1 cM-TPRSMV1-7.3 cM-Satt516-2.0 cM-TSRSMV1-4.5 cM-Sat_133. This research provides the useful information for breeders to select the two types of SMV resistance simultaneously in soybean breeding through molecular marker-assisted selection （MAS）.

关键词： soybean SMV seed coat mottling genetic analysis SSR gene mapping