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Endosymbiotic pathogen-inhibitory gut bacteria in three Indian Major Carps under polyculture system: A step toward making a probiotics consortium

作者： Darry ring

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Aquaculture and Fisheries 2021年第2期6卷 192-204页

作者： Koushik Ghosh Anjan Mukherjee Dipanjan Dutta Sudeshna Banerjee Eva Marie Breines Ellinor Hareide Einar ringø Aquaculture Laboratory Department of ZoologyThe University of BurdwanBurdwan713104India Department of Arctic and Marine Biology Faculty of BioscienceFisheries and EconomicsUiTThe Arctic University of NorwayBreivika9037Norway Norwegian College of Fishery Science Faculty of BiosciencesFisheries and EconomicsUiTThe Arctic University of NorwayTromsø9037Norway

The gastrointestinal(GI)microbiome in fish plays significant roles in health and disease *** investigation was accomplished to enumerate,characterize and identify the potential probiotic bacteria from three Indian Major Carps(IMCs),viz.,rohu(Labeo rohita),catla(Catla catla)and mrigal(Cirrhinus mrigala)using culture dependent ***,105 pathogen-inhibitory bacteria(out of 1216 isolates)were detected from three IMCs by double layer assay.16S rRNA partial gene sequence analyses and BLAST search in the NCBI GenBank unveiled that 94.29%of the pathogen inhibitory bacteria were bacilli(99 strains)and Bacillus licheniformis by far the most common(28%).The primarily selected 27 pathogen-inhibitory strains(cumulative inhibition score≥13)produced extracellular enzymes,while 15 of them produced all the six exo-enzymes studied(amylase,protease,lipa.e,cellulase,phytase and xylanase).Gut stability of the strains became apparent by their ability to grow in fish mucus and tolerance to diluted ***,14 strains were noticed asγ-hemolytic and susceptible to the commonly used ***,intra-peritoneal injection withγ-hemolytic strains did not induce any pathological signs or mortalities in fish,and thus were considered as *** 14γ-hemolytic isolates were represented by the genus Bacillus(13)and Stenotrophomonas(1),which might form probiotic consortia for prospective use in carp culture.

关键词： Carp Bacillus Stenotrophomonas Antagonism Exoenzymes 16S rRNA

Trom Mean Teld to Rdalivisic Mean Teld Theorg Progress,Success and Problems

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Trom Mean Teld to Rdalivisic Mean Teld Theorg Progress,Succe...

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor *** growing pigs by transcriptomics, metabolomics and lipidomics

CCAST“放射性束核和天体物理”研讨会暨99年亚原子物理暑期讲习班

作者： P.ring

Ⅰ. InkoductionⅡ. Mean Field Methods in Nuclear Physics ·Maocroscopic-Microscoplc Approaches ·Energy-Fuchonal Methods (Skyrme,Yogny) ·Rclafivislic Mean FieldⅢRolafing Mean Fields and fligh Spin PhysicsⅣ. Yeneralized Mean Field and Pairing (HFB-Theory)Ⅴ. Applieafions To Exofic NucleiⅥ. Time-Depend Mean,FieldsⅦ. Problems with Mean Field-Apprvximafions

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Journal of Animal Science and Biotechnology 2020年第2期11卷 527-544页

作者： Sandra Meyer Denise K.Gessner Maria S.Braune Theresa Friedhoff Erika Most Marcus Höring Gerhard Liebisch Holger Zorn Klaus Eder Robert ringseis Institute of Animal Nutrition and Nutrition Physiology Justus Liebig University GiessenHeinrich-Buff-Ring 26-3235392 GiessenGiessenGermany Institute of Clinical Chemistry and Laboratory Medicine University Hospital of RegensburgFranz-Josef-Strauss-Allee 1193053 RegensburgGermany Institute of Food Chemistry and Food Biotechnology Justus Liebig University GiessenHeinrich-Buff-Ring 1735392 GiessenGiessenGermany Fraunhofer Institute for Molecular Biology and Applied Ecology Winchester Str.235394 GiessenGiessenGermany

Background:The hypothesis was tested that insect meal(IM)as protein source influences intermediary metabolism of growing *** test this,30 male,5-week-old crossbred pigs were randomly assigned to 3 groups of 10 pigs each with similar body weights(BW)and fed isonitrogenous diets either without(CON)or with 5%IM(IM5)or 10%IM(IM10)from Tenebrio molitor *** 4 weeks and key metabolic tissues(liver,muscle,plasma)were analyzed using ***:Most performance parameters did not differ across the groups,whereas ileal digestibilities of most amino acids were 6.7 to 15.6%-units lower in IM10 than in CON(P<0.05).Transcriptomics of liver and skeletal muscle revealed a total of 166 and 198,respectively,transcripts differentially expressed between IM10 and CON(P<0.05).Plasma metabolomics revealed higher concentrations of alanine,citrulline,glutamate,proline,serine,tyrosine and valine and a lower concentration of asparagine in IM10 than in CON(P<0.05).Only one out of fourteen quantifiable amino acid metabolites,namely methionine sulfoxide(MetS),in plasma was elevated by 45%and 71%in IM5 and IM10,respectively,compared to CON(P<0.05).Plasma concentrations of both,major carnitine/acylcarnitine species and bile acids were not different across *** of liver and plasma demonstrated no differences in the concentrations of triacylglycerols,cholesterol and the main phospholipids,lysophospholipids and sphingolipids between *** percentages of all individual phosphatidylcholine(PC)and phosphatidylethanolamine(PE)species in the liver showed no differences between groups,except those with 6 double bonds(PC 38:6,PC 40:6,PE 38:6,PE 40:6),which were markedly lower in IM10 than in CON(P<0.05).In line with this,the percentage of C22:6n-3 in hepatic total lipids was lower in IM10 than in the other groups(P<0.05).Conclusions:Comprehensive analyzes of the transcriptome,lipidome and metabolome of key metabolic tissues indicate that partial or complete replacement o

关键词： Animal nutrition Insect meal Lipidome Liver Metabolism Metabolome Pigs Plasma Skeletal muscle Transcriptome

A Relation-Based Modeling Method for Workshop Reconfiguration

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International Journal of Plant Engineering and Management 2004年第4期9卷 205-214页

作者： lipa.-ring QINXian-sheng WANGKe-qin LIMin NorthwesternPolyteehniealUniversity Xi＇an710072P.R.China NorthwesternPolyteehniealUniversity Xi＇an710072P.R.China

To respond to the changes in the m arket rapidly, the workshop has become an ever-changing dynamic environment in regard to personnel change and organization alternation, etc. Therefore it is ne cessary to reconfigure the workshop system. In this paper, we present the point of view that the closer the relations are among elements in the system, the clos er they should be connected with each other when they are integrated in designin g and structural modeling of the workshop system. At first, this paper discusses the relationship among elements in the workshop system and events describing th e relationship, and provides a technical overview of the expression, definition and classification of relationship. This paper focuses on the steps and algorith m to evaluate the degree of closeness of relations among elements in systems, an d emphasizes the modeling methods for workshop reconfiguration by use of a fuzzy cluster. In light of the above steps and methods, types and contents of basic r elationships among elements should be determined, and a standard relation tree s hould be set up. Then, correlation coefficients are calculated by the standard r elation tree, and a fuzzy relation matrix is built up. After that, the structure modeling of the workshop equipment system can be completed through a fuzzy clus ter. The paper ends with an application of a FMS(Flexible Manufactuing System) f unction system modeling. Results of the modeling and calculations are presented.

关键词： relation equipment reconfiguration modeling

The Fish Microbiota:Research Progress and Potential Applications

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Engineering 2023年第10期29卷 137-146页

作者： Yinyin Luan Ming Li Wei Zhou Yuanyuan Yao Yalin Yang Zhen Zhang Einar ringø Rolf Erik Olsen Jihong Liu Clarke Shouqi Xie Kangsen Mai Chao Ran Zhigang Zhou China–Norway Joint Lab on Fish Gastrointestinal Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Norway–China Joint Lab on Fish Gastrointestinal Microbiota Institute of BiologyNorwegian University of Science and TechnologyTrondheim 7491Norway NIBIO Norwegian Institute of Bioeconomy ResearchÅs 1431Norway State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed Wuhan 430072China The Key Laboratory of Mariculture(Ministry of Education) Ocean University of ChinaQingdao 266003China

The gut microbiota plays an important role in host health and *** understanding of the fish microbiota lags far behind our knowledge of that of humans and other ***,research has highlighted the importance of the microbiota in the health,performance,and various physiological functions of *** microbiota has been studied in various fish species,including model animals,economic fish,and wild fish *** composition of the fish microbiota depends on host selection,diet,and environmental *** intestinal microbiota affects the nutritional metabolism,immunity,and disease resistance of the fish host,while the host regulates the intestinal microbiota in a reciprocal way through both immune and non-immune *** and novel gnotobiotic fish models have been developed,which are important for the mechanistic study of host–microbiota interactions in *** this review,we discuss recent progress in fish microbiota *** describe various aspects of this research,including both studies on fish microbiota variations and fundamental research extending our knowledge of host–microbiota interaction in *** on how fish microbiota research may benefit fish health and industrial sustainability are also discussed.

关键词： Gut microbiota Fish Host-microbiota interaction Aquaculture

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能源政策如何作用工业绿色经济发展

中国人口·资源与环境 2018年第12期28卷 19-28页

作者：曾婧婧童文思中南财经政法大学公共管理学院湖北武汉430073

经过长期发展,中国已形成多层次全方位的能源政策体系和多元化的能源政策目标,其中新能源开发类政策、节能类政策以及传统能源转型类政策作为三类较为主要的能源政策,分别对应能源领域开源、节流与转型的政策目标。为探明三类能源政策... 详细信息

经过长期发展,中国已形成多层次全方位的能源政策体系和多元化的能源政策目标,其中新能源开发类政策、节能类政策以及传统能源转型类政策作为三类较为主要的能源政策,分别对应能源领域开源、节流与转型的政策目标。为探明三类能源政策作用工业绿色经济发展的政策效力、政策时效性以及政策覆盖面,本文选取单位GDP能耗作为工业绿色经济发展水平的代理变量,构建能源政策作用工业绿色经济发展的计量模型,由Cobb-Douglas生产函数,将经济结构、资本与劳动力水平、地区环境治理水平、国有能源比作为控制变量纳入模型。在此基础上,使用31个省份2007年至2014年的面板数据,分区域实证检验三类能源政策以及各控制变量对中国工业绿色经济发展的作用效果。实证结果表明,各因素对绿色经济发展的作用如下:(1)开源类、节流类、转型类政策都有效促进了中国工业绿色经济发展,其中开源类政策和节流类政策的政策效力较强;(2)开源类、节流类政策的时效性强,但开源类政策更多具有短期效果,节流类政策具有持续性,转型类政策需要较长时间才能发挥作用;(3)分区域来看,节流类具有最广的政策覆盖面,在西部地区,各类政策的起效存在一年的时滞;(4)环境治理对工业绿色经济发展影响显著,治污投资比对中西部工业绿色经济的影响符合环境库兹涅茨曲线,但目前的国有能源工业规模对于工业绿色经济发展具有抑制作用。因此,本文建议应进一步推进转型类政策的合理制定,加强其政策有效性;对于西部地区,应加大基础设施建设和固定资产投资,培养有良好政策反映度的工业环境;最后,促进中国能源产业从资本密集型向知识密集型转变。

关键词：能源政策绿色经济开源节流转型

Chiral carbon dots:synthesis,optical properties,and emerging applications

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Light(Science & Applications) 2022年第4期11卷 542-564页

作者： Aaron Doring Elena Ushakova Andrey L.Rogach Department of Materials Science and Engineering and Centre for Functional Photonics(CFP)City University of Hong Kong83 Tat Chee AvenueKowloonHong Kong SARChina. Center of Information Optical Technologies ITMO UniversitySaint Petersburg 197101Russia. Shenzhen Research Institute City University of Hong Kong518057 ShenzhenChina

Carbon dots are luminescent carbonaceous nanoparticles that can be endowed with chiral properties,making them particularly interesting for biomedical applications due to their low cytotoxicity and facile *** recent years,synthetic efforts leading to chiral carbon dots with other attractive optical properties such as two-photon absorption and circularly polarized light emission have *** start this review by introducing examples of molecular chirality and its origins and providing a summary of chiroptical spectroscopy used for its *** approaches used to induce chirality in nanomaterials are *** the main part of this review we focus on chiral carbon dots,introducing their fabrication techniques such as bottom-up and top-down chemical syntheses,their morphology,and optical/chiroptical *** then consider emerging applications of chiral carbon dots in sensing,bioimaging,and catalysis,and conclude this review with a summary and future challenges.

关键词： properties synthesis chiral

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广西肝癌患者乙型肝炎病毒前C区基因的突变

中华预防医学杂志 2000年第2期34卷 83-85页

作者：方钟燎何并文庄辉 Ling ring Tim J Harrison 南宁市广西壮族自治区卫生防疫站肝炎室 530021 广西肿瘤病防治研究所北京医科大学 Royal Free Hospital University of LondonEngland

目的　了解乙型肝炎病毒 (HBV)前C区基因突变与HBeAg阴性的原发性肝癌患者HBV感染的关系。方法　应用套式PCR方法对广西 16例原发性肝癌患者血清HBVDNA前C区进行扩增 ,对阳性者用SangerDNA测序法分析。结果　 16例肝癌患者中有 14例HBV... 详细信息

目的　了解乙型肝炎病毒 (HBV)前C区基因突变与HBeAg阴性的原发性肝癌患者HBV感染的关系。方法　应用套式PCR方法对广西 16例原发性肝癌患者血清HBVDNA前C区进行扩增 ,对阳性者用SangerDNA测序法分析。结果　 16例肝癌患者中有 14例HBVDNA阳性 ,阳性率为87 5 % (14/16 )。其中 1例HBeAg阳性 ,1例虽然HBeAg为阴性 ,但其前C区序列正常 ;2例为HBV野毒株和突变株混合感染 ;其余 8例为HBV突变株感染。该 8例中 ,仅第 4、5、12例的第 2 8密码为终止密码 ,有 1例为HBV终止密码株和无终止密码突变株混合感染。结论　经典的 1896位核苷酸点突变株感染在广西原发性肝癌患者中并不常见 ,提示可能存在前C区以外的基因突变 ,并导致HBeAg阴性的HBV感染。

关键词：乙型肝炎病毒基因突变肝肿瘤