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教育研究前沿（中英文版） 2020年第2期10卷 91-98页

作者： juan lin XuanWei Zhou School of Life Sciences Fudan University200438ShanghaiP.R.China School of Agriculture and Biology Shanghai Jiao Tong University200240ShanghaiP.R.China

Genetics is a major course in Life Sciences,and also a basic course in other disciplines or specialties,such as Agricultural Sciences,Medical Sciences and *** experiments are not only the important content of the undergraduate teaching in Life Science but also the most common and efficient teaching approach in Life *** on an introduction to reform and innovation of genetics experiment instruction,this article aims at discussing how to classify experimental modules and teaching *** our experimental test,the whole experiments consist of three modules:general experiments,integrated experiments,and research-based *** research-based experiment is given as an *** herein figure out the instruction of experiment contents and guidance of learning methods,corresponding to various requirements in different teaching *** our opinion,post-graduates can act as teaching-assistants,which will facilitate undergraduates to participate in genetic experiments,improve their ability to operate by hand,and foster their innovative consciousness and scientific-thinking ability.

关键词： Genetics Experiment Teaching System Modules Teaching Strategy

YM155 inhibits retinal pigment epithelium cell survival through EGFR/MAPK signaling pathway

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International Journal of Ophthalmology(English edition) 2021年第4期14卷 489-496页

作者： Teng Li Hong-Bing Zhang Jia-Min Meng Bo Yuan Wen-juan lin Yue Feng Xiao-Dong Chen First Affiliated Hospital of Northwest University Northwest UniversityXi’an 710069Shaanxi ProvinceChina College of Life Sciences Northwest UniversityXi’an 710069Shaanxi ProvinceChina Department of Ophthalmology Xi’an No.l HospitalXi’an 710002Shaanxi ProvinceChina Shaanxi Institute of Ophthalmology Shaanxi Provincial Key Lab of OphthalmologyClinical Research Center for Ophthalmology Diseases of Shaanxi ProvinceXi’an 710002Shaanxi ProvinceChina

AIM:To investigate YM155’s effect on retinal pigment epithelium(RPE)cells’viability and the potential regulatory ***:Human immortalized RPE cell lines(ARPE-19 cell line)were processed with YM155 and epidermal growth factor(EGF).ARPE-19 cell viability was detected by methyl thiazolyl tetrazolium assay,and apoptosis was tested by flow cytometry ***-19 cell proliferation was assessed with bromodeoxyuridine tagged incorporation assay,and migration ability was evaluated via a wound-healing *** growth factor receptor(EGFR)/MAPK pathway proteins were tested via *** localization was examined by immunofluorescence ***:YM155 suppressed ARPE-19 cells’viability in a time and concentration-dependent manner.A high dose of YM155 caused a small amount of ARPE-19 cell ***155 significantly diminished the ARPE-19 cells’proliferative and migrative ***155 downregulated total EGFR and phosphorylated external signalregulated protein kinase(ERK),and it up-regulated the phosphorylation of P38 MAPK and c-Jun N-terminal kinase(JNK).YM155 induced endocytosis of EGFR in ARPE-19 ***155 also attenuated EGF-induced ARPE-19 cells’proliferative and migrative ***,YM155 significantly decreased the expression of phosphorylated EGFR and ERK after treated by ***:YM155 inhibits RPE cell survival,the cell proliferative and migrative capacity,and it effectuates a small amount of cell death through the EGFR/MAPK signaling ***155 might,therefore,be an agent to prevent and treat abnormal RPE cell survival in proliferative vitreoretinopathy.

关键词： YM155 retinal pigment epithelial cell epidermal growth factor receptor mitogen-activated protein kinase

Role of Parameter Setting in Electroacupuncture: Current Scenario and Future Prospects

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Chinese Journal of Integrative Medicine 2022年第10期28卷 953-960页

作者： ZHANG Yuan-yuan CHEN Qi-liang WANG Qiong DING Shan-shan LI Shu-nan CHEN Shu-jiao lin Xue-juan LI Can-dong Tetsuya Asakawa College of Acupuncture and Moxibustion Fujian University of Traditional Chinese MedicineFuzhou(350122)China Research Base of Traditional Chinese Medicine Syndrome Fujian University of Traditional Chinese MedicineFuzhou(350122)China Hangzhou Changgentang Clinic of Traditional Chinese Medicine Hangzhou(310009)China Department of Neurosurgery Hamamatsu University School of MedicineHandayamaHamamatsu-cityShizuoka(431-3192)Japan

Acupuncture is an ancient therapeutic method based on the theory of Chinese medicine(CM). Traditional acupuncture has many limitations;it is subjective and relies more on the experience of an acupuncturist, and the efficacy is sometimes irreproducible. In contrast, electroacupuncture(EA) has special characteristics in terms of objectivity and stability, thereby gaining considerable attention. Parameter setting plays a crucial role in EA practice. The current paper summarizes the current situation and limitations of parameter setting in EA practice. Objectification is the tendency and future of CM as well as EA. With the development of computerized technologies, such as wearable sensors, vast data, and artificial intelligence, CM syndromes can be successfully objectified. We propose the development of a novel self-feedback-adjust EA system, which may improve the parameter setting in EA and be beneficial to both the patients and clinicians.

关键词： acupuncture electroacupuncture parameter stimulation Chinese medicine artificial intelligence

Effect of Different Irrigation Patterns on Transportation and Allocation of Carbohydrate During Grain Filling of Rice

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Agricultural Sciences in China 2005年第3期4卷 181-186页

作者： CHENHai-sheng TAOLong-xing WANGXi HUANGXiao-lin TANHui-juan CHENGShi-hua MINShao-kai StateKeyLaboratoryofRiceBiology／ChinaNationalRiceResearchInstitute Hangzhou310006P.R.China

Using intersubspecific hybrid rice Xieyou 9308 and Liangyou Peijiu as the tested materials, the effects of differentirrigation patterns on transportation and allocation of carbohydrate during grain filling stage was observed by the designwith three level of soil water content in irrigated field. The results showed that in the conventional flooding and thealternate dry-watering cultivations, the exported rate of stored carbohydrate from stem and photophate from the leaves were60 and 90 % respectively. The exported rate of carbohydrate was decreased significantly (Pling grains were the major sinks for carbohydrate storage during grainfilling stage. Grains received nearly 50% of stored carbohydrate from leaf sheath and 80% of photophate from leaves. Atthe nonflooding condition the absorbing rates of grains were significantly decreased by 10 % from leaf sheath and 20 %from leaves photophate. Water stress leaded much decrease in absorbing ability for inferior grains, which might be one ofthe main causes for low seed-settling rate in non-flooding cultivation.

关键词： Rice Grain filling stage Irrigation mode Grain Photophate

Dissipativity Analysis for Markovian Jump Neural Networks With Time-Varying Delay via An Extended Relaxed Integral Inequality

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Dissipativity Analysis for Markovian Jump Neural Networks Wi...

沪深股市和香港股市的风险溢出效应研究——基于时变ΔCoVaR模型的分析

第37届中国控制会议

作者： lin Wen-juan HE Yong School of Automation China University of Geosciences Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems

This paper investigates the problem of the strictly（Q,S,R）-γ-dissipativity analysis for Markovian jump neural networks with a time-varying *** employing an appropriate Lyapunov-Krasovskii functional and using the extended relaxed integral inequality to estimate its derivative,a delay-dependent and mode-dependent condition that guarantee the considered Markovian jump neural networks strictly（Q,S,R）-γ-dissipative is ***,a numerical example is provided to illustrate the effectiveness of the proposed method.

关键词： Neural networks (Q,S,R)-γ-dissipativity Lyapunov-Krasovskii functional Extended relaxed integral inequality

来源： cnki会议评论

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系统工程理论与实践 2020年第6期40卷 1533-1544页

作者：林娟赵海龙厦门大学经济学院金融系厦门361005 “计量经济学”教育部重点实验室(厦门大学) 厦门361005 厦门大学王亚南经济研究院厦门361005

本文基于时变ΔCoVaR模型,对2006年11月至2018年12月间沪深股市和香港股市的尾部风险溢出效应进行了估算.研究发现:1)沪深股市与香港股市之间存在着双向风险溢出效应,且溢出效应均为正;2)香港股市对沪深股市的风险溢出效应强于沪深股市... 详细信息

本文基于时变ΔCoVaR模型,对2006年11月至2018年12月间沪深股市和香港股市的尾部风险溢出效应进行了估算.研究发现:1)沪深股市与香港股市之间存在着双向风险溢出效应,且溢出效应均为正;2)香港股市对沪深股市的风险溢出效应强于沪深股市对香港股市的风险溢出效应;3)深市和香港股市之间的风险溢出效应的波动幅度大于沪市和香港股市之间的风险溢出效应的波动幅度;4)沪港通和深港通的开通并没有显著增加香港股市与沪深股市之间的双向风险外溢程度.

关键词：时变VaR ΔCoVaR 尾部风险风险溢出分位数回归

Redesign of a short-chain dehydrogenase/reductase for asymmetric synthesis of ethyl (R)-2-hydroxy-4-phenylbutanoate based on per-residue free energy decomposition and sequence conservatism analysis

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Green Synthesis and Catalysis 2020年第2期1卷 150-159页

作者： Bingmei Su Lian Xu Xinqi Xu Lichao Wang Aipeng Li juan lin Lidan Ye Hongwei Yu College of Chemical Engineering Fuzhou UniversityFuzhou 350116China College of Biological Science and Engineering Fuzhou UniversityFuzhou 350116China Institute of Bioengineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027China School of Life Sciences Northwestern Polytechnical UniversityXi’an 710072China

As an important building block for the synthesis of angiotensin-converting enzyme inhibitors,ethyl(R)-2-hy-droxyl-4-phenylbutanoate[(R)-HPBE]has attracted increasing *** key to industrial biosynthesis of(R)-HPBE is a biocatalyst that efficiently reduces ethyl 2-oxo-4-phenylbutanoate(OPBE)with high *** paper proposed a strategy for identifying key residues involved in enantioselectivity control based on per-residue free energy decomposition and sequence conservatism *** this strategy,4 noncon-servative sites with high energy contribution to binding of OPBE were chosen as engineering targets,generating variant Mu27 with 99%conversion and 98%(R)ee value at substrate loading of up to 500 mmol/*** simu-lations suggested the higher stability and formation probability of Mu27-OPBEproR prereaction state as key rea-sons for the excellent R-enantioselectivity of Mu27 towards *** success in this study provides a viable approach for rational design of alcohol dehydrogenases with high enantioselectivity towards unnatural substrates.

关键词： Ethyl(R)-2-hydroxy-4-phenylbutanoate Rational design Enantioselectivity Short-chain dehydrogenase/reductase Per-residue free energy decomposition Sequence conservatism analysis

一般周期Riemann边值问题关于跳跃曲线的稳定性

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数学杂志 2011年第6期31卷 1103-1108页

作者：林娟福建商业高等专科学校基础部福建福州350012 武汉大学数学与统计学院湖北武汉430072

本文研究了一般周期Riemann边值问题关于跳跃曲线的稳定性.利用解析函数边值理论和不等式分析理论,获得了一般周期Riemann边值问题的解及其关于跳跃曲线的误差估计.

关键词：周期Riemann边值问题开口弧段摄动稳定性

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间断系数Riemann边值问题的稳定性

武汉大学学报（理学版） 2011年第2期57卷 109-114页

作者：林娟武汉大学数学与统计学院湖北武汉430072 福建商业高等专科学校基础部福建福州350012

当边界曲线发生微小的光滑扰动时,本文给出了扰动后的间断系数Riemann边值问题的解的状况,借助核密度具有弱奇性的Cauchy型积分关于积分曲线的稳定性,讨论了间断系数Riemann边值问题的解的稳定性,并给出误差估计.

关键词：间断系数 Riemann边值问题光滑摄动稳定性

Correlation of serum GDF-15 and MIF contents with the malignant behaviors of cancer cells in patients with NSCLC

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Journal of Hainan Medical University 2018年第8期24卷 40-43页

作者： Tian-Ming Zheng Li-juan lin Thoracic Surgery Department Kashgar Prefecture First People's Hospital in the Xinjiang Uygur Autonomous RegionKashgarXinjiang844000 Clinical Teaching and Research Office Kashgar Prefecture Health School in the Xinjiang Uygur Autonomous RegionKashgarXinjiang844000

Objective:To study the correlation of serum GDF-15 and MIF contents with the malignant behaviors of cancer cells in patients with ***: Patients with non-small cell lung cancer who underwent surgical resection in Kashgar Prefecture First People's Hospital in the Xinjiang Uygur Autonomous Region between January 2015 and November 2017 were selected as lung cancer group for the research, and healthy volunteers who received physical examination in Kashgar Prefecture First People's Hospital in the Xinjiang Uygur Autonomous Region during the same period were selected as the control group. Serum was collected from the lung cancer group before surgery and from the control group during physical examination respectively to determine the contents of GDF-15 and MIF;lung cancer tissue and adjacent tissue were collected from lung cancer group after surgery to determine the expression of tumor suppressor genes, proliferation genes and invasion ***:Serum GDF-15 and MIF contents of lung cancer group were significantly higher than those of control group;TCF21, Bax, GRPC5A and PTEN mRNA expression in lung cancer tissue were significantly lower than those in the adjacent tissue whereas Bcl-2, AQP4, c-myc, CyclinD1, SIRT1, CatL, MMP9, N-cadherin and Vimentin mRNA expression were significantly higher than those in adjacent tissue;serum GDF-15 and MIF contents of patients with lung cancer were negatively correlated with TCF21, Bax, GRPC5A and PTEN mRNA expression, and positively correlated with Bcl-2, AQP4, c-myc, CyclinD1, SIRT1, CatL, MMP9, N-cadherin and Vimentin mRNA expression in lung cancer ***:The abnormal increase of GDF-15 and MIF in patients with NSCLC is closely related to the abnormal proliferation and invasion of cancer cells.

关键词： Non-small cell lung cancer Growth differentiation factor 15 Macrophage migration inhibitory factor Proliferation Invasion