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Frontiers of Medicine 2024年第4期18卷 757-761页

作者： Juan wang Dongni shi Yaochen wang Xuanqi Zhang Han Li Xihong wang Shufeng Luo Lihan Hu Jiashuai Deng lin Zhang Chung Tai Lau Chung Wah Cheng Fei Han Ji Li Ping wang Aiping Lyu Zhaoxiang Bian Xuan Zhang institute for Research and Continuing Education Hong Kong Baptist UniversityShenzhen518057China chinese EQUATOR Centre Hong Kong Baptist UniversityHKSAR999077China School of Traditional chinese Medicine Tianjin University of Chinese MedicineTianjin301617China School of chinese Medicine Beijing University of Chinese MedicineBeijing100105China School of chinese Medicine Hong Kong Baptist UniversityHKSAR999077China Department of Pediatrics Guang’anmen HospitalChina Academy of Chinese Medical SciencesBeijing100053China Xiyuan Hospital China Academy of Chinese Medical SciencesBeijing100091China Centre for chinese Herbal Medicine Drug Development Hong Kong Baptist UniversityHKSAR999077China

To enhance the clarity and transparency of reporting in trials involving chineseherbal medicine(CHM)formulas,the CONSORT extension specifically tailored for CHM formulas(CONSORT-CHM Formulas 2017)was introduced in 2017[1].This statement,along with its corresponding checklist,outlines key elements essential for reporting in randomized controlled trials(RCTs)related tothe rationale,design,analysis,and interpretation of interventions using CHM *** CONSORT-CHM Formulas 2017 elaborated on 7 of 25 CONSORT checklist items,namely title,abstract and keywords,backgrounds and objectives,participants,interventions,outcomes,generalizability,and interpretation.

关键词： formulas statement rational

AlphaFold-guided redesign of a plant pectin methylesterase inhibitor for broad-spectrum disease resistance

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Molecular Plant 2024年第9期17卷 1344-1368页

作者： Yeqiang Xia Guangzheng Sun Junhua Xiao Xinyi He Haibin Jiang Zhichao Zhang Qi Zhang Kainan Li Sicong Zhang Xuechao shi Zhaoyun wang lin Liu Yao Zhao Yuheng Yang Kaixuan Duan Wenwu Ye Yiming wang Suomeng Dong Yan wang Zhenchuan Ma Yuanchao wang Department of Plant Pathology Nanjing Agricultural UniversityNanjingJiangsu 210095China Key Laboratory of Soybean Disease and Pest Control(Ministry of Agriculture and Rural Affairs) Nanjng Agricultural UniversityNanjngJiangsu 210095China Anhui Province Key Laboratory of Crop Integrated Pest Management Anhui Agricultural UniversityHefei 230036China School of Bioengineering Dalian University of TechnologyDalian 116024China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejing 100193China These authors contributed equally to the article.

Plant cell walls are a critical site where plants and pathogens continuously struggle for physiological *** we show that dynamic remodeling of pectin methylesterification of plant cell walls is a compo-nent of the physiological and co-evolutionary struggles between hosts and pathogens.A pectin methyles-terase(PsPME1)secreted by Phytophthora sojae decreases the degree of pectin methylesterification,thus synergizing with an endo-polygalacturonase(PsPG1)to weaken plant cell *** counter PsPME1-mediated susceptibility,a plant-derived pectin methylesterase inhibitor protein,GmPMl1,protects pectin to maintain a high methylesterification ***1 protects plant cell walls from enzymatic degrada-tion by inhibiting both soybean and *** pectin methylesterases during ***,constitutive expression of GmPMl1 disrupted the trade-off between host growth and defense *** therefore used AlphaFold structure tools to design a modified form of GmPMI1(GmPMI1R)that specifically targets and inhibits pectin methylesterases secreted from pathogens but notfrom *** expression of GmPMi1R enhanced plant resistance to oomycete and fungal *** summary,our work highlights the biochemical modification of the cell wall as an important focal point in the physiological and co-evolutionary conflict between hosts and microbes,providing an important proof of concept that Al-driven structure-based tools can accelerate the development of new strategies for plant protection.

关键词： apoplastic immunity AlphaFold-guided redesign broad-spectrum disease resistance pectin methyl-esteraseinhibitor cell wall

热带低丘地区农场尺度土壤质量指标的空间变异

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土壤通报 2003年第4期34卷 241-245页

作者：张华张甘霖中国科学院南京土壤研究所江苏南京210008

本文以我国热带地区海南儋州一个典型农场为样区,采用地统计学方法研究土壤质量指标的空间变异。研究选择了土壤质量评价最小数据集中的7项土壤质量指标(总有机碳,容重,粘粒含量,速效P、K,阳离子交换量,pH)进行分析,统计分析的结果表明... 详细信息

本文以我国热带地区海南儋州一个典型农场为样区,采用地统计学方法研究土壤质量指标的空间变异。研究选择了土壤质量评价最小数据集中的7项土壤质量指标(总有机碳,容重,粘粒含量,速效P、K,阳离子交换量,pH)进行分析,统计分析的结果表明,研究区域土壤存在酸化和养分贫瘠化现象。地统计学分析结果表明,表层土壤基底值普遍高于表下层土壤,说明表层土壤由于受随机因素影响更为强烈导致空间变异性增强。在各项土壤质量指标中,pH和土壤容重主要表现为基底效应,总有机碳、阳离子交换量,速效P、K含量以及亚表层土壤粘粒含量表现出中等空间相关性,表层粘粒含量空间变异随距离增加而增加。在GIS支持下,采用块段克立格方法(blockkriging)进行各项土壤质量指标的空间内插得到指标的等级分布图。研究结果可以为进一步的土壤质量综合评价提供方法和理论依据。

关键词：热带低丘地区农场尺度土壤质量指标空间变异地统计学地理信息系统

Storage and Spatial Variation of Phosphorus in Paddy soils of China

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Pedosphere 2009年第6期19卷 790-798页

作者： lin Jin-shi shi Xue-Zheng LU Xi-Xi YU Dong-Sheng wang Hong-Jie ZHAO Yong-Cun SUN Wei-Xia State Key Laboratory of soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 2100(78 (China) Department of Geography National University of Singapore 119260 (Singapore) Graduate University of the chinese academy of sciences Beijing 100049 (China) College of Resources and Environment Fujian Agriculture and Forestry University Fuzhou 350002 (China)

Due to the growing concern about the agricultural phosphorus (P) losses pollution, an in-depth understanding of P in paddy soils of China would be helpful in providing a national perspective of the environmental impact of P cycling and fertility on China's farms. In this study, we evaluated the P storage and the P density of paddy soils in China, characterized the spatial variations of P among the subgroups of paddy soils and soil regions in China, and evaluated the P data using GIS-based analysis, which included a newly compiled 1:1 000 000 digital soil map of China, and using 1 490 soil profiles. The available and total P densities of paddy soils were 6.7 and 698.5 g m -3 , respectively. Overall in China, the total P storage within 1 m of paddy soils was estimated to be 330.2 Tg. The P density of paddy soils varied substantially with subgroups due to the different soil water regimes such as groundwater table and soil drainage. The P availability in paddy soils, especially in surface layer, was higher in high temperature and precipitation areas. Further research is needed to examine more anthropogenic impact factors, such as increasing use of chemical fertilizer.

关键词： paddy soil database paddy soil subgroups phosphorus density phosphorus pool

Dissolved organic matter accelerates microbial degradation of 17 alpha-ethinylestradiol in the presence of iron mineral

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Journal of Environmental sciences 2024年第5期139卷 364-376页

作者： Huan He Min shi Xiaoxia Yang Juhong Zhan Yanting lin Ziwei Guo Zhicheng Liao Chaochao Lai Xiaomin Ren Bin Huang Xuejun Pan Faculty of Environmental science and Engineering Kunming University of Science and TechnologyKunming 650500China Yunnan Provincial Key Lab of soil Carbon Sequestration and Pollution Control Kunming 650500China Research institute for Environmental Innovation(Suzhou)Tsinghua Suzhou 215163China

Dissolved organic matter(DOM)and iron minerals widely existing in the natural aquatic environment can mediate the migration and transformation of organic ***,the mechanism of interaction between DOM and iron minerals in the microbial degradation of pollutants deserves further *** this study,the mechanism of 17 alphaethinylestradiol(EE2)biodegradation mediated by humic acid(HA)and three kinds of iron minerals(goethite,magnetite,and pyrite)was *** results found that HA and iron minerals significantly accelerated the biodegradation process of EE2,and the highest degradation efficiency of EE2(48%)was observed in the HA-mediated microbial system with pyrite under aerobic ***,it had been demonstrated that hydroxyl radicals(HO·)was the main active substance responsible for the microbial degradation of ***·is primarily generated through the reaction between hydrogen peroxide secreted by microorganisms and Fe(II),with aerobic conditions being more *** presence of iron minerals and HA could change the microbial communities in the EE2 biodegradation *** findings provide new information for exploring the migration and transformation of pollutants by microorganisms in iron-rich environments.

关键词： Dissolved organic matter Iron mineral 17 alpha-ethinylestradiol Biodegradation

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内外生菌根真菌对重金属的耐受性及机理

土壤 2003年第5期35卷 370-377页

作者：廖继佩林先贵曹志洪中国科学院南京土壤研究所南京210008

本文从内外生菌根真菌对重金属的耐受性和耐受机理,以及将菌根真菌作为重金属污染程度的生物指示剂和重金属生物修复等方面对菌根真菌和重金属的相互作用作了较全面的论述。重金属对生物圈的污染是一个严重的环境和健康问题。某些内外... 详细信息

本文从内外生菌根真菌对重金属的耐受性和耐受机理,以及将菌根真菌作为重金属污染程度的生物指示剂和重金属生物修复等方面对菌根真菌和重金属的相互作用作了较全面的论述。重金属对生物圈的污染是一个严重的环境和健康问题。某些内外生菌根真菌对重金属具有耐受性。菌根真菌菌丝能与金属相结合而限制它们向菌根植物地上部迁移,从而可达到植物稳定和保护植物免遭重金属毒害的目的。内外生菌根真菌对重金属的耐受性因菌种、重金属种类和浓度、与宿主植物共生与否以及所生长的土壤条件等而异,同时种内菌株之间也有差异。菌根真菌通过离子交换,络合物的形成,沉淀或结晶化作用等方式获得对重金属的耐受性,其子实体内重金属含量,繁殖体密度和侵染势可作为重金属污染程度的生物指示剂。

关键词：外生菌根真菌内生菌根真菌重金属耐受性

Pollen and Phytolith Analyses of Ancient Paddy Fields at Chuodun Site, the Yangtze River Delta

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Pedosphere 2007年第2期17卷 209-218页

作者： LI Chun-Hai ZHANG Gang-Ya YANG lin-Zhang lin Xian-Gui HU Zheng-Yi DONG Yuan-Hua CAO Zhi-Hong ZHENG Yun-Fei DING Jin-Long nanjing institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 （China） institute of soil science Chinese Academy of Sciences Nanjing 210008 （China） Zhejiang Provincial institute of Cultural Relics and Archaeology Hangzhou 310014 （China） Suzhou Museum Suzhou 215001 （China）

A number of paddy fields pertaining to the Majiabang Cultures (5500-3800 years BC) were discovered during the archaeological excavations that were carried out since 1998 at the Chuodun site in the Yangtze River Delta. The pollen and phytolith analyses of two soil profiles from the northeastern part of this site were carried out to trace the agricultural practices of the Neolithic period. The phytolith results showed that rice domestication in the Yangtze River Delta could be traced back to as early as the Majiabang Culture. The pollen assemblage also revealed low levels of aquatic species, similar to that in modern paddy fields. This finding suggested that humans might have removed weeds for rice cultivation during the Neolithic period. Thus, pollen analysis in association with phytolith analysis was a promising method for identifying ancient paddy fields.

关键词： ancient paddy fields Neolithic period pollen analysis phytolith analysis Yangtze River Delta

Chemical and Microbiological Parameters of Paddy soil Quality as Affected by Different Nutrient and Water Regimes

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Pedosphere 2005年第3期15卷 369-378页

作者： YANGChang-Ming YANGlin-Zhang YANTing-Mei StateKeyLab.ofPollutionControlandResourceReuse CollegeofEnvironmentalScienceandEngineeringTongjiUniversityShanghai200092（China） instituteofsoilscience ChineseAcademyofSciencesP.O.Box821Nanjing210008（China）

A field experiment was conducted from 1999 to 2002 to compare and evaluate the effects of nutrient and water regimes on paddy soil quality by investigating soil chemical and microbiological parameters. Four nutrient regimes, a control, chemical fertilizers only (CF), chemical fertilizers with swine manure (SM), and chemical fertilizers with wheat straw (WS), and two soil moisture regimes, continuous waterlogging (CWL) and alternate wetting and drying (AWD), were investigated. With SM and WS total organic carbon and total nitrogen in the paddy soil were significantly higher (P soil light fraction organic C (LFOC), water-soluble carbohydrates (WSC), and water-soluble organic C (WSOC). CWL, in particular when swine manure was incorporated into the paddy soil, markedly decreased soil redox potential (Eh) and increased total active reducing substances (ARS). Meanwhile, as compared to CF, SM and WS significantly (P soil microbial biomass C (MBC) and mineralizable carbon, with differences in AWD being higher than CWL. In addition, SM and WS treatments significantly (P soils, long-term waterlogging should be avoided when organic manure was incorporated into paddy soil.

关键词： biological index of fertility nutrient regimes paddy soil quality water regimes

Targeting a disintegrin and metalloprotease(ADAM)17-CD122 axis enhances CD8^(+)T cell effector differentiation and anti-tumor immunity

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Signal Transduction and Targeted Therapy 2024年第7期9卷 3097-3111页

作者： lina Sun Anjun Jiao Haiyan Liu Renyi Ding Ning Yuan Biao Yang Cangang Zhang Xiaoxuan Jia Gang wang Yanhong Su Dan Zhang lin shi Chenming Sun Aijun Zhang Lianjun Zhang Baojun Zhang Department of Pathogenic Microbiology and Immunology School of Basic Medical SciencesXi’an Jiaotong UniversityXi’anShaanxi 710061China institute of Infection and Immunity Translational Medicine InstituteXi’an Jiaotong University Health Science CenterXi’anShaanxi 710061China Key Laboratory of Environment and Genes Related to Diseases Ministry of EducationXi’anShaanxi 710061China Xi’an Key Laboratory of Immune Related Diseases Xi’anShannxi 710061China Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer InstituteXuzhou Medical UniversityXuzhouJiangsuChina Department of Pediatrics Qilu Hospital of Shandong UniversityJinanChina National Key Laboratory of Immunity and Inflammation Suzhou Institute of Systems MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeSuzhou 215123 JiangsuChina Key Laboratory of Synthetic Biology Regulatory Elements Suzhou Institute of Systems MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeSuzhou 215123 JiangsuChina

CD8^(+)T cell immune responses are regulated by multi-layer networks,while the post-translational regulation remains largely *** ectodomain shedding is an important post-translational process orchestrating receptor expression and signal transduction through proteolytic cleavage of membrane ***,by targeting the sheddase A Disintegrin and Metalloprotease(ADAM)17,we defined a post-translational regulatory mechanism mediated by the ectodomain shedding in CD8^(+)T *** and proteomic analysis revealed the involvement of post-translational regulation in CD8^(+)T cells.T cellspecific deletion of ADAM17 led to a dramatic increase in effector CD8^(+)T cell differentiation and enhanced cytolytic effects to eliminate pathogens and ***,ADAM17 regulated CD8^(+)T cells through cleavage of membrane ***17 inhibition led to elevated CD122 expression and enhanced response to IL-2 and IL-15 stimulation in both mouse and human CD8^(+)T ***,inhibition of ADAM17 in CD8^(+)T cells improved the efficacy of chimeric antigen receptor(CAR)T cells in solid *** findings reveal a critical post-translational regulation in CD8^(+)T cells,providing a potential therapeutic strategy of targeting ADAM17 for effective anti-tumor immunity.

关键词： immunity stimulation involvement

用Guelph法研究不同土地利用方式下富铁土的土壤渗透性

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土壤侵蚀与水土保持学报 1998年第4期4卷 13-18页

作者：于东升史学正中国科学院南京土壤研究所

利用Ｇｕｅｌｐｈ入渗仪研究了我国南方低丘不同土地利用方式下富铁土的土壤渗透性，结果表明，同种利用方式的０５ｃｍ表层土壤饱和入渗率Ｋｆｓ值下坡大于上坡，且植被覆盖度越高，表层土壤的Ｋｆｓ值也越大。不同利用方式的表层土壤... 详细信息

利用Ｇｕｅｌｐｈ入渗仪研究了我国南方低丘不同土地利用方式下富铁土的土壤渗透性，结果表明，同种利用方式的０５ｃｍ表层土壤饱和入渗率Ｋｆｓ值下坡大于上坡，且植被覆盖度越高，表层土壤的Ｋｆｓ值也越大。不同利用方式的表层土壤Ｋｆｓ值之间的关系表现为：人工种植的草地或薪炭林＞旱作或经济林及农林间作土壤＞自然草地＞侵蚀裸地；翻耕法土壤＞免耕法土壤或无耕作的土壤。

关键词： Guelph法土壤渗透性土地利用方式 Guelph入渗仪土壤饱和入渗率