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Responses of plant rhizosphere to atmospheric CO_2.enrichment

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Chinese Science Bulletin 2000年第2期45卷 97-101页

作者： lin weihong, ZHANG Fusuo & BAI Kezhi Institule of Bolany, Chinese Academy of Sciences, {2. 100093, {2.: department of plant nutrition, {2. AgriculturaI university, beijing 100094, {2. Institute of Botany Chinese Academy of Sciences Beijing China department of plant {2. China Agricultural University Beijing China

plant root growth is generally stimulated under elevated CO2. This will bring more carbon to the below-ground through root death and exudate. This potential increase in below-ground carbon sink may lead to changes in long-term soil sequestration and relationship between host plants and symbions. On the other hand, changes in litter components due to the changes in plant chemical composition may also affect soil processes, such as litter decomposition, soil organic matter sequestration and hetero-nutritional bacteria activities. These issues are discussed.

关键词： CO2 eurichment rhizosphere microorganism.

Carbon isotope ratios of C_(4) plants in loess areas of North china

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Science china Earth Sciences 2006年第1期49卷 97-102页

作者： wang Guoan1,2.3, HAN Jiamao 2. ZHOU Liping3, XIONG Xiaogang4, TAN Ming2. WU Zhenhai5 & PENG Jun6 1. department of {2. nutrition, china agricultural university, beijing 100094, china 2. Institute of Geology and Geophysics, Chinese Academy of Sciences, beijing 10002., china 3. College of Environmental Sciences, MOE Laboratory for Earth Surface Processes, Peking university, beijing 100871, china 4. Institute of Botany, Chinese Academy of Sciences, beijing 100093, china 5. Northwest Sci-Tech university of Agriculture and Forestry, Yangling 712.00, china 6. Network Center, china agricultural university, beijing 100094, china 1. department of plant {2. China Agricultural University Beijing 100094 China 2. Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China 3. College of Environmental Sciences MOE Laboratory for Earth Surface Processes Peking University Beijing 100871 China 4. Institute of Botany Chinese Academy of Sciences Beijing 100093 China 5. Northwest Sci-Tech university of Agriculture and Forestry Yangling 712100 China 6. Network Center China Agricultural University Beijing 100094 China

Carbon isotope ratios (δ^(13)C) of 89 C_4 plant samples were determined fromthe loess area in North china. δ^(13)C values vary between -10.5per thousand and -14.6per thousandwith a mean of -12.6per thousand. Along a precipitation gradient from the semi-moist area to thesemiarid area, then to the arid area, the δ^(13)C values of C_4 plants show a slight decreasingtrend. The δ^(13)C values of C_4 plants in the dry season are found lower than those in the wetseason. These trends are opposite to those observed for C_3 species.

关键词： C4 plants, carbon isotope ratios, loess area.

Evaluation of Pathway of Nitrogen Loss in Winter Wheat and Summer Maize Rotation System

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agricultural Sciences in china 2002年第11期1卷 1224-1231页

作者： JU Xiao-tang, LIU Xue-jun, ZOU Guo-yuan, wang Zhao-hui and ZHANG Fu-suo(department of {2. nutrition , china agricultural university , beijing 100094 ,P. R. china) department of plant {2. China Agricultural University Beijing 100094P.R.China

The nitrogen loss pathway in winter wheat and summer maize rotation system was studied based on field experimental data. The results showed that nitrogen recovery rate was significantly decreased with nitrogen fertilization rate increased, while residual rate and losses rate had an increasing trend. Accumulated ammonia volatilization loss in winter wheat and summer maize rotation was 12. 8(N0), 2..0(N12.), 33. 0(N2.0) and 64. 5 kg N ha-1 (N360) respectively and rate of ammonia volatilization loss was 3.8, 4.2.and 7.2. respectively while urea was mixed with 0 -10 cm soil or spread before irrigation. Denitrification loss with acetylene-soil core incubation method in winter wheat was lower than 1 kg N ha-1 and rate of denitrification loss was 0. 2. - 0. 2.% or trace. Denitrification loss in summer maize was 1 - 14 kg N ha-1 and rate of denitrification loss was 1-5%. The total gaseous loss in winter wheat and summer maize rotation system was less than 10%, and the main nitrogen fertilizer loss way was leaching below 0 - 100 cm soil profile and accumulated in deeper soil.

关键词： Winter wheat Summer maize Ammonia volatilization Denitrif ication Nitrate leaching

NextPolish2.A Repeat-aware Polishing Tool for Genomes Assembled Using HiFi Long Reads

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Genomics, Proteomics & Bioinformatics 2024年第1期22卷 105-109页

作者： Jiang Hu Zhuo wang Fan Liang Shan-lin Liu Kai Ye De-Peng wang School of Automation Science and Engineering Faculty of Electronic and Information EngineeringXi’an Jiaotong UniversityXi’an 710049China GrandOmics Biosciences Beijing 102206China department of Entomology College of Plant ProtectionChina Agricultural UniversityBeijing 100193China

The high-fidelity(HiFi)long-read sequencing technology developed by PacBio has greatly improved the base-level accuracy of genome ***,these assemblies still contain base-level errors,particularly within the error-prone regions of HiFi long *** genome polishing tools usually introduce overcorrections and haplotype switch errors when correcting errors in genomes assembled from HiFi long ***,we describe an upgraded genome polishing tool-NextPolish2.which can fix base errors remaining in those“highly accurate”genomes assembled from HiFi long reads without introducing excessive overcorrections and haplotype switch *** believe that NextPolish2.has a great significance to further improve the accuracy of telomere-to-telomere(T2.)***2.is freely available at https://***/Nextomics/NextPolish2.

关键词： Telomere-to-telomere Genome assembly Genome polishing Error correction HiFi long read

Identification and Practical Application of Silicate-dissolving Bacteria

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agricultural Sciences in china 2002年第1期1卷 81-85页

作者： lin Qi-mei, RAO Zheng-hua, SUN Yan-xing, YAO Jun and XING Li-jun( department of Soil and Water Sciences ,{2.,{2. Institute of plant nutrition and Resources , beijing Academy of agricultural and Forestry Sciences, beijing 100081 ) department of Soil and Water Sciences China Agricultural University Beijing 100094 Institute of plant nutrition and Resources Beijing Academy of Agricultural and Forestry Sciences Beijing 100081

Slime-forming bacteria were isolated from soils, rock surface and earthworm intestine, and their effects on dissolving silicate minerals and tomato growth were examined. One of the bacteria, Bacillus mucilaginosus RGBc13, had particularly strong ability to form slime and dissolve silicates. RGBc13 could also colonize and develop in both non-rhizosphere and rhizosphere soil. Total number of slime-forming bacteria increased from 2.9 × 103 cfu·g-1and 8.4 × 103 cfu·g-1 to 9.6 × 106 cfu·g-1 and 6.0 × 107 cfu·g-1 in the non-rhizosphere and rhizosphere soils respectively. Potassium and phosphorus nutritional conditions in the rhizosphere were markedly improved through inoculation of this bacterium. Available K and P respectively increased from 2.. 86 and 3. 63mg · kg-1 in the non-rhizosphere soil to 91. 2. and 5. 74mg · kg-1 in the rhizosphere soil. Tomato biomass increased by 12.%, K and P uptakes were more than 150%, greater than the non-inoculation. Thus, there is a potential in applying RGBcl3 for improving plant K and P nutrition.

关键词： Silicate-dissolving bacteria Silicates Tomato

Composite structure and properties of the pitcher surface of the carnivorous plant Nepenthes and its influence on the insect attachment system

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Progress in Natural Science:Materials International 2009年第12期19卷 1657-1664页

作者： Lixin wang,Qiang Zhou~*,Yongjun Zheng,Shuyan Xu department of Mechatronic Engineering,{2. agricultural university,beijing 100083,{2. [a]department of Mechatronic Engineering China Agricultural University Beijing 100083 China

Pitchers of the carnivorous plant Nepenthes have evolved specialized organs serving the purpose of attracting,capturing,retaining and digesting small animals,mostly *** consist of several well distinguishable zones,including a leaf-like lid,a collar-like peristome, a slippery zone and a digestive zone,differing in morphology,microstructure,chemical composition and physical *** zones display different functions,and the combined effects of several zones result in great trapping *** principal aim of this review is to introduce the structure and physiochemical properties of the pitcher surface,as well as the interaction between the pitcher surface and the insect attachment *** with our present study,the potential application of the pitcher surface being utilized in bionics to manufacture insect slippery trapping plates is discussed,and the original research direction of the pitcher surface and its application on agricultural pest control is highlighted.

关键词： Pitcher surface Nepenthes Composite structure Insect Attachment system

Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica(Phaeophyta)

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Acta Oceanologica Sinica 2012年第6期31卷 139-148页

作者： XUAN Jinsong 1,4,FENG Yanbin 1,WENG Manli 1,ZHAO Ge 1,SHI Jinfeng 1,YAO Jianting 2.wang Xiuliang 2.GUO Baotai 3,QIAO Lixian 3,DUAN Delin 2.wang Bin 1 1 The State Key Laboratory of {2. Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,beijing 100101,china 2.The State Key Laboratory of Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 2.6071,china 3 College of Life Sciences,Qingdao agricultural university,Qingdao 2.6109,china 4 department of Biological Science and Engineering,School of Chemical and Biological Engineering,university of Science and Technology beijing,beijing 100083,china 1. The State Key Laboratory of plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 4. department of Biological Science and Engineering School of Chemical and Biological Engineering University of Science and Technology Beijing Beijing 100083 China 2. The State Key Laboratory of Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 3. College of Life Sciences Qingdao Agricultural University Qingdao 266109 China

A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2.1. 5 pfu/*** average insert size of the cDNA library is about 1.6 *** the cDNA library,591 cDNA clones were randomly selected and *** a result,574 EST(expressed sequence tag) sequences were *** of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942.2. to *** cDNA library was screened with a α-32.p labeled 453 bp T P S gene probe,which is a partial sequence yielded from Porphyra *** positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of *** TPS cDNA *** PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named *** encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 *** LjTPS gene was successfully expressed in *** and *** LjTPS gene has potential application both in {2. breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance.

关键词： cDNA library comparative study expressed sequence tag(EST) analysis Laminaria japonica trehalose-6-phosphate synthase(TPS) T P S gene

Biogenic nanostructured silica

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Progress in Natural Science:Materials International 2002年第6期 3-8页

作者： wang Lijun, wang Yunhua, LI Min, ZHANG Wenxu, ZHANG Fusuo and LI Tiejin(1. {2. of Resources, Environments & Agrochemistry, Huazhong agricultural university, Wuhan 430070, china 2. {2. of plant nutrition, china agricultural university, beijing 100094, china 3. {2. of Chemistry, Jilin university, Changchun 13002., china) department of Resources，Environments & Agrochemistry，Huazhong agricultural {2.，Wuhan 430070，china department of plant {2.，china agricultural university，beijing 100094，china department of Resources，Environments & Agrochemistry，Huazhong agricultural {2.，Wuhan 430070，china department of plant {2.，china agricultural university，beijing 100094，china department of Chemistry，Jilin {2.，Changchun 13002.，{2.

Silicon is by far the most abundant element in tbe earth crust and also is an essential element for higher plants, yet its biology and mechanisms in plant tolerance of biotic and abiotic stresses are poorly understood. Based on the molecular mechanisms of the biosilicification in marine organisms such as diatoms and sponges, the cell wall template-mediated self-assembly of nanostructured silica in marine organisms and higher plants as well as the related organic molecules are discussed. Understanding of the templating and structure-directed effects of silicon-processing organic molecules not only offers the clue for synthesizing silicon-based materials, but also helps to recognize the anomaly of silicon in plant biology.

关键词： biogenic silica nanostructures silica proteins biomineralization.

Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages

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Chinese Science Bulletin 2003年第19期48卷 2068-2071页

作者： LIU Fengxia, SUN Chuanqing, TAN Lubin, FU Yongcai, LI Dejun & wang Xiangkun department of {2. Genetics and Breeding, china agricultural university, National Key Laboratory of Agrobiology, Key Laboratory of Crop Genetic Improvement and Genome of the Ministry of Agriculture, beijing 100094, china department of plant Genetics and Breeding China Agricultural University National Key Laboratory of Agrobiology Key Laboratory of Crop Genetic Improvement and Genome of the Ministry of Agriculture China

An advanced backcross population of rice was used to identify the quantitative trait locus (QTL) controlling the cold-tolerance at booting to flowering stages. The recipient, Guichao 2.(GC2., was a commercial Indica rice; the donor Dongxiang common wild rice, was an accession of common wild rice (DXCWR, Oryza rufipogon Griff.). Three QTLs for cold-tolerance were detected on chromosomes 1, 6 and 11. Two of them coming from DXCWR could enhance the cold-tolerance of the backcross progenies. Moreover, one sterility QTL that could reduce the seed set rate of the backcross progenies by 78% was mapped on chromosome 5.

关键词：耐寒性水稻开花期播种设备回交杂种 QTL分析数量特点轨迹