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Chinese Science Bulletin 2009年第24期54卷 4664-4670页

作者： LU XiaoQing HAN JinRun LIU XiaoFang lin tonghui LI Yulin HU XiaoXiang LI Ning State Key Laboratory of Agrobiotechnology College of Biological Sciences China Agricultural University Beijing 100193 China College of Animal Science & Technology China Agricultural University Beijing 100193 China

Endogenous viruses integrate into the host genome and influence the expression of neighboring genes through their long terminal repeats (LTRs). In this study, we analyzed the promoter, enhancer and transcriptional activities of the chicken endogenous retrovirus ev21 LTR. The LTR was cloned into pGL3-basic and pGL3-promoter luciferase vectors in forward and reverse orientation separately. The luciferase activities were detected respectively in chicken embryonic fibroblast cell, human embryonic kidney cell line, human lung carcinoma cell line, Chinese hamster ovary cell line, and murine melanoma cell line. Relative luciferase activity analysis indicated that the ev21 LTR retained bi-directional promoter activity but no detectable enhancer activity in these cells. The constructs containing the LTR and F1 region show stronger promoter activity than the constructs containing only LTR. The transcriptional pattern of ev21 LTR varied in tissues of late feathering White Leghorn chicken at post-hatch day one. Skin exhibits the highest expression in the tissues examed. Collectively, our results indicate that the ev21 LTR exhibits tissue-type specific expression in White Leghorn chicken, and it also have regulatory potential.

关键词：内源性逆转录病毒启动子活性白来航鸡组织特异性展品铁胚胎成纤维细胞宿主基因组

股前外侧穿支皮瓣在电烧伤治疗中应用的研究进展

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中华损伤与修复杂志（电子版） 2024年第6期19卷 526-530页

作者：林同辉杨卫玺南京医科大学附属淮安第一医院烧伤整形外科 223300

高压电烧伤常导致广泛软组织损伤和严重血管栓塞,破坏组织解剖层次深,特别是合并肌肉、肌腱、神经损伤时,患肢坏死风险高,临床修复难度大,致残率和截肢率高,严重影响患者的生存质量。传统的修复方法多采用反复清创、培养肉芽和二期植皮... 详细信息

高压电烧伤常导致广泛软组织损伤和严重血管栓塞,破坏组织解剖层次深,特别是合并肌肉、肌腱、神经损伤时,患肢坏死风险高,临床修复难度大,致残率和截肢率高,严重影响患者的生存质量。传统的修复方法多采用反复清创、培养肉芽和二期植皮修复,但受区易发生并发症且外观美学功能差。随着显微外科技术的发展,穿支皮瓣游离移植成为修复电烧伤软组织缺损的重要手段之一。股前外侧穿支皮瓣具有血管恒定、切取范围大、美观性能好且供区损伤小等优点,因此越来越多地应用于电烧伤创面修复。本文对股前外侧穿支皮瓣在电烧伤治疗中应用的研究进展进行综述。

关键词：烧伤,电股前外侧穿支皮瓣旋股外侧动脉降支修复

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义乌市某水厂扩建工程设计及其运行效果

广东化工 2020年第1期47卷 49-51页

作者：遇光禄林彤晖周华中国电建集团华东勘测设计研究院有限公司

义乌市某水厂原供水规模为5×104 m3/d,二期扩建工程实施后处理规模增加至10×104 m3/d。扩建工程采用"折板絮凝/平流沉淀池+气水反冲洗均粒滤料滤池"工艺,通过优化絮凝池等构筑物的形式、调整药剂投加点位及投加量、增加设备配置,出水... 详细信息

义乌市某水厂原供水规模为5×104 m3/d,二期扩建工程实施后处理规模增加至10×104 m3/d。扩建工程采用"折板絮凝/平流沉淀池+气水反冲洗均粒滤料滤池"工艺,通过优化絮凝池等构筑物的形式、调整药剂投加点位及投加量、增加设备配置,出水水质更加稳定,且出水浊度由≤0.7 NTU变为≤0.1 NTU,达到浙江省现代化水厂标准。该工程有效解决了水厂供水能力不足、出水水质波动大等问题,可为类似水厂的建设提供借鉴。

关键词：水厂扩建工程设计

韧性城市视角下日本排水管网抗震设计体系与启示

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市政技术 2023年第8期41卷 289-295页

作者：吴舒畅王亮林彤晖林天扬周望开朱世俊中国电建集团华东勘测设计研究院有限公司浙江杭州311122 浙江省华东生态环境工程研究院浙江杭州311122 浙江大学建筑设计研究院有限公司浙江杭州310028 日本工程咨询公司东京1010022 浙江工业大学土木工程学院浙江杭州310014

排水管网是生命线工程的重要组成部分,过去的地震灾害表明排水管网抗震能力非常薄弱,而地震时排水管网受损会造成极大的社会经济损失。日本作为地震多发国家,吸取了其东北地区太平洋近海地震的教训,改变了过去仅以防灾为手段的抗震方针... 详细信息

排水管网是生命线工程的重要组成部分,过去的地震灾害表明排水管网抗震能力非常薄弱,而地震时排水管网受损会造成极大的社会经济损失。日本作为地震多发国家,吸取了其东北地区太平洋近海地震的教训,改变了过去仅以防灾为手段的抗震方针,通过总结多年抗震实践经验,逐步形成了以抗震目标为导向,合理设定功能要求优先级,防灾为主、防灾减灾相结合的排水管网抗震设计体系,并总结出一系列因地制宜的抗震手段和强化措施。值得我国在排水管网抗震设计工作中学习借鉴。

关键词：排水管网韧性城市地震抗震设计日本

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HPLC法测定还原型谷胱甘肽原料药有关物质

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药学研究 2018年第9期37卷 506-510页

作者：林彤慧陈娟王东武孙丽宁刘茜山东绿叶制药有限公司质量控制部山东烟台264003

目的建立一种测定还原型谷胱甘肽中有关物质的反相高效液相色谱法。方法色谱柱:Agilent Zorbax SB-Aq;以磷酸盐缓冲溶液-甲醇体系为流动相;流速为1.0 m L·min^(-1);柱温为30℃;检测波长为210 nm。结果该方法精密度试验RSD≤5.0%;谷胱... 详细信息

目的建立一种测定还原型谷胱甘肽中有关物质的反相高效液相色谱法。方法色谱柱:Agilent Zorbax SB-Aq;以磷酸盐缓冲溶液-甲醇体系为流动相;流速为1.0 m L·min^(-1);柱温为30℃;检测波长为210 nm。结果该方法精密度试验RSD≤5.0%;谷胱甘肽及杂质A、B、C、D在线性范围内线性相关系数r≥0.999 7,检出限分别为0.002 9、0.003 4、0.003 1、0.005 5、0.004 1μg,定量限分别为0.005 8、0.006 7、0.006 1、0.011、0.008 1μg;加标回收率分别为100.9%、99.4%、98.2%、103.2%。结论该方法准确、灵敏,适用于谷胱甘肽中有关物质的检测。

关键词：谷胱甘肽有关物质高效液相色谱法杂质

Identification of Cinobufagin and Resibufogenin as Inhibitors of Enterovirus 71 Infection

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Chemical Research in Chinese Universities 2014年第6期30卷 953-958页

作者： CHEN Jiawen XU lin SUN Shiyang ZHANG Huafei MA tonghui SU Weiheng JIANG Chunlai National Engineering Laboratory for AIDS Vaccine Key Laboratory for Molecular Enzymology & Engineering Ministry of Education School of Life Seiences Jilin University Changchun 130012 P. R. China" College of Basic Medical Sciences Dalian Medical University Dalian 116044 t~ R. China

In this paper, cinobufagin and resibufogenin were found to inhibit enterovirus 71（EV71） infection in vitro in cell viability and plaque reduction assays. The 50% inhibitory concentrations（lCs0） of einobufagin and resibufoge- nin were （10.94±2.36） and （218±31） nmol/L, respectively, the 50% cytotoxic concentrations（CCs0） of them were （1277±223） and （1385±254） nmol/L, respectively, and the anti-EV71 selectivity index（SI50） of cinobufagin was 116.7, which are promisingly developed into drug. Using a VP1 detection assay and a constructed reporter luciferase, we found that cinobufagin and resibufogenin disrupted the synthesis of EV71 protein. However, neither of them inhibited EV71 RNA replication. Our study suggests that cinobufagin and resibufogenin are the promising candidates that should he fllrther investigated for the treatment of EV71 caused disease.

关键词： Enterovirus 71 Coxsackievirus A16 Cinobufagin Resibufogenin Chansu

Facile Modification on Buried Interface for Highly Efficient and Stable FASn_(0.5)Pb_(0.5)I_(3) Perovskite Solar Cells with NiOx Hole-Transport layers

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Chinese Journal of Chemistry 2023年第23期41卷 3197-3204页

作者： Hui Zhang Yuan Zhou tonghui Guo Xiang Zhang Zhenkun Zhu Junjun Jin Xiaxia Cui Dan Zhang Zhen Wang lin Li Nai Wang Guanqi Tang Qidong Tai The Institute of Technological Sciences Wuhan UniversityWuhanHubei430072 China Research Institute of Frontier Science Southwest Jiaotong UniversityChengduSichuan610031 China College of Science Henan University of EngineeringZhengzhouHenan451191 China

Formamidinium(FA)-based Sn-Pb perovskite solar cells(FAPb_(0.5)Sn_(0.5)I_(3) PSCs)with ideal bandgap and impressive thermal stability have caught enormous attention ***,it still suffers from the challenge of realizing high efficiency due to the surface imperfections of the transport materials and the energy-level mismatch between functional ***,it is demonstrated that the modification on buried interface with alkali metal salts is a viable strategy to alleviate these *** systematically investigate the role of three alkali metal bromide salts(NaBr,KBr,CsBr)by burying them between the NiOx hole transport layer(HTL)and the perovskite light-absorbing layer,which can effectively passivate interface defects,improve energy-level matching and release the internal residual strain in perovskite *** device with CsBr buffer layer exhibits the best power conversion efficiency(PCE)approaching 20%,which is one of the highest efficiencies for FA-based Sn-Pb PSCs employing NiO_(x) ***,the long-term storage stability of the unencapsulated device is also greatly *** work provides an efficient strategy to prepare desired FA-based ideal-bandgap Sn-Pb PSCs which could be applied in tandem solar cells.

关键词： Tin-lead perovskite solar cell Formamidinium Alkali metal bromide Efficiency Interfacial modification Stability

A mechanism for the origin and development of the large-scale dunefield on the right flank of the lower reach of Laoha River,Northeast China

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Research in Cold and Arid Regions 2015年第1期7卷 29-39页

作者： Guang Han GuiFang Zhang Li You Liang Zhou lin Yang XueYong Zhao Yulin Li tonghui Zhang Hunan Normal University Hunan University Climatic Center of Inner Mongolia Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences

By viewing satellite imagery, a striking large-scale dunefield can be clearly perceived, with a size of nearly 63 km long and 11 km wide, and trending NE-SW, on the right flank of the lower Laoha River, Northeast China. By means of remote sensing imagery analysis and field observation as well as a comparison with a small-scale dunefield on the right flank of the lower Xiangshui River, analogous to the case of the lower Laoha River, this paper presents a new mechanism for its origin and development. The results show that：（1） the large-scale dunefield bears a tile-style framework overwhelmingly composed of transverse barchanoid ridges perpendicular to the predominant winds, and inlaid diverse blowouts. （2） The small-scale dunefield, referred to as a primary structural unit of the large one, is typical of an incipient dunefield, following the same rules of evolution as the larger. （3） A succession of barchanoid ridge chains can steadily migrate downwind in much the same manner as surface wave propagation in air or water stimulated by an incised valley, and ultimately tend to bear roughly the same wavelength and amplitude under stable climate and hydrologic regimes. （4） The first ridge chain acquires its sand source substantially from the downwind escarpments exposing the loose Quaternary sandy sediments to the air, while the ensuing ridges derive their sands dominantly from in situ deflation of the underlain Quaternary loose sandy sediments in blowouts, partly from the upwind ridges through northern elongated horns. Theoretically, the sands from riparian escarpments can be transported by wind to the downwind distal end of a dunefield after sufficient long du- ration. （5） The lower Laohahe region experienced probably three significant climatic changes in the past, corresponding to the three active dune belts, suggesting that once a large-scale dunefield occurs, it is nearly impossible to be completely stabilized, at least in its central portions. At present, seasonal s

关键词： lower Laoha River large-scale dunefield wavy barchanoid ridges topographic perturbation blowouts

MDGA2 Constrains Glutamatergic Inputs Selectively onto CA1 Pyramidal Neurons to Optimize Neural Circuits for Plasticity,Memory,and Social Behavior

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Neuroscience Bulletin 2024年第7期40卷 887-904页

作者： Xuehui Wang Donghui lin Jie Jiang Yuhua Liu Xinyan Dong Jianchen Fan Lifen Gong Weida Shen linghui Zeng tonghui Xu Kewen Jiang Steven A.Connor Yicheng Xie School of Life Sciences Nanchang UniversityNanchang330031China Department of Neurology Children's HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Biology York University4700 Keele StreetTorontoON M3J 1P3Canada Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province School of MedicineHangzhou City UniversityHangzhou 310015China

Synapse organizers are essential for the development,transmission,and plasticity of *** as rare synapse suppressors,the MAM domain containing glycosylphosphatidylinositol anchor(MDGA)proteins contributes to synapse organization by inhibiting the formation of the synaptogenic neuroligin-neurexin complex.A previous analysis of MDGA2 mice lacking a single copy of Mdga2 revealed upregulated glutamatergic synapses and behaviors consistent with ***,MDGA2 is expressed in diverse cell types and is localized to both excitatory and inhibitory *** the network versus cell-specific effects of MDGA2 loss-of-function requires a cell-type and brain region-selective *** address this,we generated mice harboring a conditional knockout of Mdga2 restricted to CA1 pyramidal *** we report that MDGA2 suppresses the density and function of excitatory synapses selectively on pyramidal neurons in the mature *** deletion of Mdga2 in CA1 pyramidal neurons of adult mice upregulated miniature and spontaneous excitatory postsynaptic potentials,vesicular glutamate transporter 1 intensity,and neuronal *** effects were limited to glutamatergic synapses as no changes were detected in miniature and spontaneous inhibitory postsynaptic potential properties or vesicular GABA transporter ***,evoked basal synaptic transmission and AMPAR receptor currents were enhanced at glutamatergic *** a behavioral level,memory appeared to be compromised in Mdga2 cKO mice as both novel object recognition and contextual fear conditioning performance were impaired,consistent with deficits in long-term potentiation in the CA3-CA1 *** affiliation,a behavioral analog of social deficits in autism,was similarly *** results demonstrate that MDGA2 confines the properties of excitatory synapses to CA1 neurons in mature hippocampal circuits,thereby optimizing this network for plasticity,c

关键词： MDGA2 CA1 pyramidal neurons Glutamatergic inputs Synaptic plasticity Memory Social behaviors Autism Synapse organizers

Word Sense Disambiguation Model with a Cache-Like Memory Module

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Journal of Donghua University(English Edition) 2021年第4期38卷 333-340页

作者： lin Qian LIU Xin XIN Chunlei ZHANG Haiying ZENG Hualin ZHANG tonghui SU Jinsong School of Informatics Xiamen UniversityXiamen 361005China

Word sense disambiguation(WSD),identifying the specific sense of the target word given its context,is a fundamental task in natural language ***,researchers have shown promising results using long short term memory(LSTM),which is able to better capture sequential and syntactic features of ***,this method neglects the dependencies among instances,such as their context semantic *** solve this problem,we proposed a novel WSD model by introducing a cache-like memory module to capture the semantic dependencies among instances for *** evaluations on standard datasets demonstrate the superiority of the proposed model over various baselines.

关键词： word sense disambiguation(WSD) memory module semantic dependencies