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抗大别班达病毒人源单克隆抗体Fab片段的噬菌体展示文库构建

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中国病原生物学杂志 2024年第11期19卷 1251-1254,1259页

作者：徐家馨王静静王琳徐睿杰吴玉龙李波清乔媛媛滨州医学院基础医学院病原生物学教研室山东烟台264003

目的构建具有潜在中和活性的人源抗大别班达病毒(SFTSV)抗体Fab片段的噬菌体展示文库。方法从具有交叉中和活性的SFTS恢复期患者血液样本中分离外周血单个核细胞(PBMC)并提取总RNA,逆转录PCR得到cDNA。以cDNA为模板进行第一轮PCR扩增,... 详细信息

目的构建具有潜在中和活性的人源抗大别班达病毒(SFTSV)抗体Fab片段的噬菌体展示文库。方法从具有交叉中和活性的SFTS恢复期患者血液样本中分离外周血单个核细胞(PBMC)并提取总RNA,逆转录PCR得到cDNA。以cDNA为模板进行第一轮PCR扩增,分别获得抗体重链可变区(VH)和轻链可变区(Vκ和Vλ)序列。以pComb3XTT、pComb3Xλ质粒为模板,分别获得抗体重链恒定区(CH1)和轻链恒定区(Cκ、Cλ)序列。第二轮通过重叠延伸PCR(SOE-PCR)扩增VH和CH1得到Fd重链,同样扩增Vκ和Cκ、Vλ和Cλ得到完整的κ和λ抗体轻链。第三轮以上述扩增产物为模板再次进行SOE-PCR,得到完整的抗体Fab基因片段。将Fab基因片段插入到pComb3XSS噬菌粒中,构建噬菌体展示抗体文库。结果通过三轮PCR扩增出抗体Fab片段的序列,成功构建库容量为1.875×10^(9)的噬菌体展示抗体文库。结论利用具有交叉中和活性的SFTS恢复期患者血源建立了人源抗SFTSV Fab片段的噬菌体展示抗体文库,为下一步筛选特异性单克隆中和抗体建立了可靠的前期基础。

关键词： SFTSV 噬菌体展示抗体文库库容量人源单克隆抗体

Luteolin suppresses oral carcinoma 3(OC3) cell growth and migration via modulating polo-like kinase 1(PLK1) expression and cellular energy metabolism

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第12期24卷 1151-1158页

作者： Pengfei GAO Wentao ZHANG Yujie lin ruijie LU Zijian LOU Gang LU Ruolang PAN Yunfang CHEN Center for Plastic&Reconstructive Surgery Department of StomatologyZhejiang Provincial People's Hospital(Affiliated People's Hospital)Hangzhou Medical CollegeHangzhou 310014China School of Stomatology Bengbu Medical CollegeBengbu 233030China The Second Clinical Medical College Wenzhou Medical UniversityWenzhou 325035China Key Laboratory of Cell-Based Drug and Applied Technology Development in Zhejiang Province Hangzhou 31122China

Oral squamous cell carcinoma(OSCC)is a prevalent malignant tumor affecting the head and neck region(Leemans et al.,2018).It is often diagnosed at a later stage,leading to a poor prognosis(Muzaffar et al.,2021;Li et al.,2023).Despite advances in OSCC treatment,the overall 5-year survival rate of OSCC patients remains alarmingly low,falling below 50%(Jehn et al.,2019;Johnson et al.,2020).According to statistics,only 50%of patients with oral cancer can be treated with *** discovered,it is more frequently at an advanced *** addition,owing to the aggressively invasive and metastatic characteristics of OSCC,most patients die within one year of ***,the pursuit of novel therapeutic drugs and treatments to improve the response of oral cancer to medication,along with a deeper understanding of their effects.

关键词： PLK1 metabolism drugs

The role of transporters in cancer redox homeostasis and cross-talk with nanomedicines

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Asian Journal of Pharmaceutical Sciences 2020年第2期15卷 145-157页

作者： Longfa Kou Xinyu Jiang Huirong Huang Xinlu lin Youting Zhang Qing Yao ruijie Chen Department of Pharmacy The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou 325027China School of Pharmaceutical Sciences Wenzhou Medical UniversityChashanWenzhou 325035China

Tumor cell usually exhibits high levels of reactive oxygen species and adaptive antioxidant system due to the metabolic,genetic,and microenvironment-associated *** altered redox homeostasis can promote tumor progression,development,and treatment *** membrane transporters are involved in the resetting redox homeostasis and play important roles in tumor ***,targeting the involved transporters to disrupt the altered redox balance emerges as a viable strategy for cancer *** addition,nanomedicines have drawn much attention in the past *** nanomedicines to target or reset the redox homeostasis alone or combined with other therapies has brought convincing data in cancer *** this review,we will introduce the altered redox balance in cancer metabolism and involved transporters,and highlight the recent advancements of redox-modulating nanomedicines for cancer treatment.

关键词： Cancer metabolism ROS,Redox homeostasis Membrane transporter xCT/SLC7A11 Nanomedicine

Liquid-FEP-based U-tube triboelectric nanogenerator for harvesting water-wave energy

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Nano Research 2018年第8期11卷 4062-4073页

作者： Lun Pan Jiyu Wang Peihong Wang ruijie Gao Yi-Cheng Wang Xiangwen Zhang Ji-Jun zou Zhong lin Wang School of Material Science and Engineering Georgia Institute of Technology Atlanta Georgia 30332 USA Key Laboratory for Green Chemical Technology of the Ministry of Education School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China 3Collaborative Innovative Center of Chemical Science and Engineering (Tianjin) Tianjin 300072 China 4Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences Beijing 100083 China Lun Pan and Jiyu Wang contributed equally to this work.

Harvesting ambient mechanical energy is a key technology for realizing self-powered electronics. With advantages of stability and durabilid, a liquid-solid-based triboelectric nanogenerator （TENG） has recently drawn much attention. However, the impacts of liquid properties on the TENG performance and the related working principle are still unclear. We assembled herein a U-tube TENG based on the liquid-solid mode and applied 11 liquids to study the effects of liquid properties on the TENG output performance. The results confirmed that the key factors influencing the output are polarity, dielectric constant, and affinity to fluorinated ethylene propylene （FEP）. Among the 11 liquids, the pure water-based U-tube TENG exhibited the best output with an open-circuit voltage （Voc） of 81.7 V and a short-circuit current （Isc） of 0.26 μA for the shaking mode （0.5 Hz）, which can further increase to 93.0 V and 0.48 μA, respectively, for the horizontal shifting mode （1.25 Hz）. The U-tube TENG can be utilized as a self-powered concentration sensor （component concentration or metal ion concentration） for an aqueous solution with an accuracy higher than 92%. Finally, an upgraded sandwich-like water-FEP U-tube TENG was applied to harvest water-wave energy, showing a high output with Voc of 350 V, Isc of 1.75 μA, and power density of 2.04 W/m3. We successfully lighted up 60 LEDs and powered a temperature-humidity meter. Given its high output performance, the water-FEP U-tube TENG is a very promising approach for harvesting water-wave energy for self-powered electronics.

关键词： triboelectric nanogenerator FEP U tube liquid properties ionic aqueous solution water-wave energy

关于全域土地综合整治的思考——以西安市为例

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农业技术与装备 2024年第1期 36-39页

作者：周林刘喆梁晓晶王锐张瑞杰杨钰陕西华地勘察设计咨询有限公司陕西西安710018 西安建筑科技大学陕西西安710055

开展全域土地综合整治是贯彻国家新时期发展要求,有效发挥优化城乡地区国土空间格局作用,助力乡村振兴的一项重要举措。结合西安市全域土地综合整治试点的实践经验,从规划编制、组织管理体系建立、配套管理制度完善、项目实施监管、产... 详细信息

开展全域土地综合整治是贯彻国家新时期发展要求,有效发挥优化城乡地区国土空间格局作用,助力乡村振兴的一项重要举措。结合西安市全域土地综合整治试点的实践经验,从规划编制、组织管理体系建立、配套管理制度完善、项目实施监管、产业导入等方面,总结了试点实施中存在的问题,提出了针对性的优化建议,以期为全域土地综合整治的全面开展提供建议和思路。

关键词：全域土地综合整治困境对策西安

近足月-足月儿生后早期肺部生理征象的超声特征分析

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中国优生与遗传杂志 2024年第4期32卷 833-837页

作者：张睿洁郤冠楠林昕奥樊晓波汪吉梅复旦大学附属妇产科医院新生儿科上海200001

目的研究足月儿或近足月儿生后早期“宫内-宫外”转换期间肺部超声图像特征和变化规律。方法采集2019年4—12月于复旦大学附属妇产科医院出生的466名足月儿或近足月儿肺部超声图像。检查的时间点为生后0.5 h、1.0 h、2.0 h和4.0 h;保留... 详细信息

目的研究足月儿或近足月儿生后早期“宫内-宫外”转换期间肺部超声图像特征和变化规律。方法采集2019年4—12月于复旦大学附属妇产科医院出生的466名足月儿或近足月儿肺部超声图像。检查的时间点为生后0.5 h、1.0 h、2.0 h和4.0 h;保留3 d后未发生肺部疾病的新生儿作为研究对象,回顾性分析他们出生时采集的图像。结果最终纳入393例无肺部疾病新生儿的肺部超声图像,分别有109例于生后0.5 h;107例于生后1.0 h;141例于生后2.0 h;36例于生后4.0 h采集超声图像。无肺部疾病的足月儿或近足月儿肺部超声的征象主要有3种:“单纯A-线”“散在B-线”和“融合B-线”。其中“融合B-线”和“散在B-线”随时间逐渐减少(P<0.001);而“单纯A-线”则逐渐增多(P<0.001)。生后1 h内有两例新生儿出现“融合B线”,分别于生后0.5 h和1 h各有1例。结论肺部超声可以动态观察足月儿或近足月儿生后早期肺部生理征象,主要有3种:“单纯A-线”“散在B-线”和“融合B-线”。需要被临床医生所重视,以免造成过度治疗。

关键词：肺超声检查足月儿近足月儿生理征象

评价胸腔镜辅助小切口手术在胸外伤治疗中的应用价值

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系统医学 2024年第7期9卷 127-130页

作者：郭华林志明樊睿杰史文武翁培兰解放军联勤保障部队庐山康复疗养中心胸心外科江西九江332000 九江学院基础医学院江西九江332000

目的评价胸腔镜辅助小切口手术在胸外伤治疗中的应用效果。方法选取解放军联勤保障部队庐山康复疗养中心于2018年1月—2022年12月收治的80例胸外伤治疗患者为研究对象,通过随机数表法分为比较组(40例,传统开胸手术)与研究组(40例,胸腔... 详细信息

目的评价胸腔镜辅助小切口手术在胸外伤治疗中的应用效果。方法选取解放军联勤保障部队庐山康复疗养中心于2018年1月—2022年12月收治的80例胸外伤治疗患者为研究对象,通过随机数表法分为比较组(40例,传统开胸手术)与研究组(40例,胸腔镜辅助小切口手术),比较两组手术指标、术后引流情况、时间指标、疼痛评分、并发症发生情况。结果研究组出血量(84.15±10.02)mL,切口长度(8.02±1.41)cm,优于比较组出血量(180.32±12.02)mL、切口长度(20.12±1.36)cm,差异有统计学意义(t=38.868、39.064,P均<0.05)。研究组术后引流情况、时间指标、疼痛评分及并发症发生情况均优于比较组,差异有统计学意义(P均<0.05)。结论胸外伤治疗中应用胸腔镜辅助小切口手术能够优化手术指标,改善引流指标,缩短康复时间,降低术后疼痛评分与并发症发生率。

关键词：胸外伤治疗胸腔镜辅助小切口手术疼痛评分手术指标术后引流情况

The effects of DS blade's geometry features on material's creep strength

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Propulsion and Power Research 2014年第3期3卷 143-150页

作者： Xiaojun Yan Kai Zhang Ying Deng ruijie Sun Lianshan lin Xiaoyong Zhang School of Energy and Power Engineering Beihang UniversityBeijing 100191China Oak Ridge National Laboratony Oak RidgeTN 37831United States

Because of the better creep performance,the directional solidification(DS)Nickel-based turbine blades have been widely used in advanced ***,the DS turbine blade's different abrupt geometrical changes at different regions cause a variation of temperature field at those ***,the variable temperature field is very likely to lead to a different grain structures at those different region,and those different grain structure finally give rise to a variation in material's creep performance at different region in DS turbine *** study the variation in creep strength among different regions of a DS turbine blade,this article designed and manufactured three types of DS specimens to simulate the geometry features of platform,shroud and body part of a typical DS turbine *** tests on these specimens were conducted under the stress level of 608 MPa and temperature of 850。C,the creep rupture life of platform-like and shroud-like specimens are 93%and 73%of body-like specimens'respectively,which support the assumption that there exists a certain variation in material's creep strength among different regions in DS turbine *** fracture positions of these specimens also support above *** is suggested that the material's creep strength variation among different locations of DS turbine blades should be considered in future turbine blade life design and prediction.

关键词： Creep strength Directional solidification Turbine blade Grain structure Geometry features

适用于光导开关触发的V/N气体开关设计与验证

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强激光与粒子束 2024年第5期36卷 68-74页

作者：宋雨辉王凌云周良骥刘宏伟张东东陈林袁建强邓明海谢卫平高彬王瑞杰大连理工大学电气工程学院辽宁大连116024 中国工程物理研究院流体物理研究所四川绵阳621900 天府创新能源研究院成都610000 四川质安高电压工程技术研究中心四川绵阳621000

为了满足多路精确同步触发开关要求,将光导开关(PCSS)与V/N气体开关结合,可充分发挥PCSS低触发阈值、低抖动和光电隔离以及V/N气体开关工作电压高、带载能力强等优势。两种开关结合的核心是V/N气体开关结构参数与PCSS触发回路的参数匹... 详细信息

为了满足多路精确同步触发开关要求,将光导开关(PCSS)与V/N气体开关结合,可充分发挥PCSS低触发阈值、低抖动和光电隔离以及V/N气体开关工作电压高、带载能力强等优势。两种开关结合的核心是V/N气体开关结构参数与PCSS触发回路的参数匹配。分析计算了V/N气体开关的结构电容、触发回路振荡参数、开关电场分布等,研究了V/N气体开关的结构电容与PCSS、串联电感等构成的振荡回路的匹配关系,通过实验获得了V/N气体开关自击穿电压曲线、导通延迟时间以及不同欠压比下的延迟时间抖动等,初步验证了适用于PCSS触发的V/N气体开关设计。

关键词：同步触发 V/N气体开关 PCSS 结构电容自击穿电压