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Lagrangian analysis of the formation and mass transport of compressible vortex rings generated by a shock tube

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Chinese Physics B 2021年第3期30卷 183-193页

作者： haiyan lin Yang Xiang Hong Liu Bin Zhang School of Aeronautics and Astronautics Shanghai Jiao Tong UniversityShanghai 200240China

In order to understand the mass transport and the dynamic genesis associated with a compressible vortex formation,a dynamic analysis of compressible vortex rings (CVRs) generated by shock tubes by using the framework of Lagrangiancoherent structures (LCSs) and finite-time Lyapunov exponents field (FTLE) is performed. Numerical calculation is performed to simulate the evolution of CVRs generated by shock tubes with 70 mm, 100 mm, and 165 mm of the driver sectionat the circumstances of pressure ratio = 3. The formation of CVRs is studied according to FTLE fields. The mass transportduring the formation is obviously seen by the material manifold reveled by FTLE fields. A non-universal formation numberfor the three CVRs is obtained. Then the elliptic LCSs is implemented on three CVRs. Fluid particles separated by ellipticLCSs and ridges of FTLE are traced back to t = 0 to identify the fluid that eventually forms the CVRs. The elliptic LCSsencompass around 60% fluid material of the advected bulk but contain the majority of the circulation of the ring. The otherparts of the ring carrying almost zero circulation advect along with the ring. Combining the ridges of FTLE and the ellipticLCS, the whole CVR can be divided into three distinct dynamic parts: vortex part, entrainment part, and advected part. Inaddition, a criterion based on the vortex part formation is suggested to identify the formation number of CVRs.

关键词： compressible vortex rings Lagrangian coherent structure mass transport

Effects of sense and antisense centromere/kinetochore complex protein-B (CENP-B) in cell cycle regulation

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Chinese Science Bulletin 2005年第24期50卷 2836-2843页

作者： LUO Song lin haiyan QI Jianguo WANG Yongchao Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life Sciences Beijing Normal University Beijing 100875 China

This paper investigates the effects of sense and antisense centromere/kinetochore complex protein-B (CENP-B) in cell cycle regulation. Full-length cenpb cDNA was subcloned into pBI-EGFP eukaryotic expression vector in both sense and antisense orientation. HeLa-Tet-Off cells were transfected with sense or antisense cenpb vectors. Sense transfection of HeLa-Tet-Off cells resulted in the formation of a large centromere/kinetochore complex, and apoptosis of cells following several times of cell division. A stable an- tisense cenpb transfected cell line, named HACPB, was ob- tained. The centromere/kinetochore complex of HACPB cells became smaller than control HeLa-Tet-Off cells and scat- tered, and the expression of CENP-B was down-regulated. In addition, delayed cell cycle progression, inhibited malignant phenotype, restrained ability of tumor formation in nude mice, and delayed entry from G2/M phase into next G1 phase were observed in HACPB cells. Furthermore, the ex- pression of cyclin-dependent kinases (CDKs), cyclins, and CDK inhibitors (CKIs) were modulated during different phases of the cell cycle. CENP-B is an essential protein for the maintenance of the structure and function of centro- mere/kinetochore complex, and plays important roles in cell cycle regulation.

关键词：细胞周期着丝粒/着丝点复合蛋白 CENP-B 反义转染

Dynamic Intra-Japonica Subspecies Variation and Resource Application

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Molecular Plant 2012年第1期5卷 218-230页

作者： haiyan lin Peng Xia Rod A.Wing Qifa Zhang Meizhong Luo National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Arizona Genomics Institute University of ArizonaTucsonAZ 85721USA

We constructed a physical map of O. sativa ssp. japonica cv. ZH11 and compared it and its random sample sequences with the Nipponbare RefSeq derived from the same subspecies. This comparison showed that the two japonica genomes were highly syntenic but revealed substantial differences in terms of structural variations, rates of substitutions and indels, and transposable element content. For example, contractions/expansions as large as 450 kb and repeat sequences that were present in high copy numbers only in ZH11 were detected. In tri-alignment regions using the indica variety 93-11 sequence as an outgroup, we found that：（1） the substitution rates of the two japonica-indica inter- subspecies comparison combinations were close but almost a magnitude higher than the substitution rate between the japonica rice varieties ZH11 and Nipponbare; （2） of the substitutions found between ZH11 and Nipponbare, 47.2% occurred in ZH11 and 52.6% in Nipponbare; （3） of the indels found between ZH11 and Nipponbare, the indels that occurred in ZH11 were 15.8 times of those in Nipponbare. Of the indels that occurred in ZH11, 75.67% were insertions and 24.33% deletions. Of the indels that occurred in Nipponbare, 48.23% were insertions and 51.77% were deletions. The ZH11 com- parative map covered four Nipponbare physical gaps, detected assembly errors in the Nipponbare sequence, and was integrated with the FSTs of a large ZH11 T-DNA insertion mutant library. ZH11 BAC clones can be browsed, searched, and obtained at our website, http：//***.

关键词： Physical map comparative genomics Japonica Oryza rice BAC.

One-pot/three-step synthesis of zinc-blende CdSe/CdS core/shell nanocrystals with thick shells

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Nano Research 2017年第4期10卷 1149-1162页

作者： Yuan Niu Chaodan Pu Runchen Lai Renyang Meng Wanzhen lin haiyan Qin Xiaogang Peng Center for Chemistry of Novel and High-Performance Materials and Department of Chemistry Zhejiang University Hangzhou 310027 China

A one-pot/three-step synthetic scheme was developed for phase-pure epitaxy of CdS shells on zinc-blende CdSe nanocrystals to yield shells with up to sixteen monolayers. The key parameters for the epitaxy were identified, including the core nanocrystal concentration, solvent type/composition, quality of the core nanocrystals, epitaxial growth temperature, type/concentration of ligands, and composition of the precursors. Most of these key parameters were not influential when the synthetic goal was thin-shell CdSe/CdS core/shell nanocrystals. The finalized synthetic scheme was reproducible at an almost quantitative level in terms of the crystal structure, shell thickness, and optical properties.

关键词： zinc-blende core/shell quantum dots thick shells

GASEOUS REACTIVE NITROGEN LOSSES FROM ORCHARDS,VEGETABLES AND TEA PLANTATIONS

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Frontiers of Agricultural Science and Engineering 2023年第2期10卷 155-166页

作者： Jinyang WANG Pinshang XU haiyan lin Shumin GUO Zhaoqiang HAN Jianwen ZOU Key Laboratory of Green and Low-carbon Agriculture in Southeastern China Ministry of Agriculture and Rural AffairsCollege of Resources and Environmental SciencesNanjing Agricultural UniversityNanjing 210095China Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource UtilizationNanjing 210095China.

Nitrogen fertilizer application has accelerated the agricultural soil N cycle while ensuring food *** reactive N emissions from orchards,vegetables and tea plantations(OVT)are less understood than those from cereal *** paper presents a compilation of data on soil ammonia,nitrous oxide,and nitric oxide emissions from 1454 OVT systems at 184 unique experimental locations worldwide aiming to investigate their emission characteristics,emission factors(EF),and contribution to total farmland ***_(3)and N_(2)O emissions from orchards and N_(2)O and NO emissions from vegetable production were significantly higher in China than in the rest of the world,regardless of fertilizer application,while N_(2)O emissions from tea plantations were lower than for *** EF of NH_(3)for vegetables was close to the global mean value with urea application but significantly higher than that of *** EF of N_(2)O in orchards and vegetables was comparable to the global median value,while in tea plantations,the value was 2.3 times higher than the global median *** estimates suggest that direct emissions of NH_(3),N_(2)O,and NO from OVT systems are equivalent to approximately a quarter,two thirds and a half of the total farmland in China,*** research needs to strengthen observational field studies in establishing standard sampling methods for gaseous N emissions and implementing knowledge-based management measures to help achieve the green development of agriculture.

关键词： fruit greenhouse gas green development fertilizer management climate change

Single nucleotide polymorphisms in the human corticosteroid-binding globulin promoter alter transcriptional activity

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Science China(Life Sciences) 2012年第8期55卷 699-708页

作者： LI Yue WU Liang LEI JingHui ZHU Cheng WANG HongMei YU XiaoGuang lin haiyan Department of Biochemistry and Molecular Biology College of Basic Medical ScienceHarbin Medical UniversityHarbin 150081China State Key Laboratory of Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China

Corticosteroid-binding globulin(CBG) is a high-affinity plasma protein that transports glucocorticoids and *** and we have reported non-synonymous single nucleotide polymorphisms(SNPs) that influence CBG production or steroid-binding ***,no promoter polymorphisms affecting the transcription of human CBG gene(Cbg) have been *** the present study we investigated function implications of six promoter SNPs,including 26 C/G,54 C/T,144 G/C,161 A/G,205 C/A,and 443/444 AG/,five of which are located within the first 205 base pairs of 5'-flanking region and close to the highly conserved footprinted elements,TATA-box,or *** reporter assays demonstrated that basal activity of the promoter carrying 54 T or 161 G was significantly *** first three polymorphisms,26 C/G,54 C/T,and 144 G/C located close to the putative hepatic nuclear factor(HNF) 1 binding elements,altered the transactivation effect of *** also found a negative promoter response to dexamethasone-activated glucocorticoid receptor(GR),although none of the SNPs affected its transrepression *** results suggest that human Cbg 26 C/G,54 C/T,144 G/C,and 161 A/G promoter polymorphisms alter transcriptional activity,and further studies are awaited to explore their association with physiological and pathological conditions.

关键词： corticosteroid-binding globulin single nucleotide polymorphism hepatic nuclear factor 1

Effects of antisense CENP-B on the proliferation of HeLa (Tet-off) cells

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Chinese Science Bulletin 2002年第5期47卷 379-383页

作者： Qianjin Liang Qian Wang haiyan lin Song Luo Chunmei Wang Dacheng He Yongchao Wang Beijing Normal Univ Coll Life Sci Beijing 100875 Peoples R China

In order to study the change of the expression of centromere protein B (CENP-B) caused by antisense transfection, proto-eukaryotically expressed fused protein GST-CENP-B (65 ku) was injected into mouse, and a peculiar anti-CENP-B serum MaCenpB was collected. A strain of transfected HeLa Tet-off cell HaCb, which contains antisense CENP-B expressing vector pBI-EGFP-as-CenpB, was prepared. Northern blot and Western blot were used to analyze the repression of internal CENP-B in transfected cells. According to the growth curve, the proliferation of HeLa (Tet-off) is repressed by antisense CENP-B, and the multiplication time is prolonged for 32.81 h. The analysis of flow cytometry revealed that, compared with HeLa (Tet-off), the G1 cell population of HaCb is increased (AG1=9%) while S fraction is decreased (AS=11%), but the G2/M phase is nearly unchanged (AG2/M=3%). In the meanwhile, the mi-totic index of HaCb declines greatly compared with that of HeLa (Tet-off). Immunofluorescence showed that the

关键词： centromere proteins CENP-B HeLa (Tet-off) cell.

GRAIN INCOMPLETE FILlinG 2 regulates grain filling and starch synthesis during rice caryopsis development

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Journal of Integrative Plant Biology 2017年第2期59卷 134-153页

作者： Xiangjin Wei Guiai Jiao haiyan lin Zhonghua Sheng Gaoneng Shao Lihong Xie Shaoqing Tang Qingguo Xu Peisong Hu State Key Laboratory of Rice Biology Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture China National Rice Research Institute Hangzhou 310006 China . College of Agronomy Hunan Agricultural University Changsha 410128 China Agricultural Genomes Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 China

Rice grain filling determines grain weight, final yield and grain quality. Here, a rice defective grain filling mutant, gif2, was identified. Grains ofgif2 showed a slower filling rate and a significant lower final grain weight and yield compared to wild-type. The starch content in gilt2 was noticeably decreased and its physicochemical properties were also altered. Moreover, gif2 endosperm cells showed obvious defects in compound granule formation. Posi- tional cloning identified GIF2 to encode an ADP-glucose pyrophosphorylase （AGP） large subunit, AGPL2; consequently, AGP enzyme activity in gif2 endosperms was remarkably decreased. GIF2 is mainly expressed in developing grains and the coded protein localizes in the cytosol. Yeast two hybrid assay showed that GIF2 interacted with AGP small subunits OsAGPS% OsAGPS2a and OsAGPS2b. Transcript levels for granule-bound starch synthase, starch synthase, starch branching enzyme and starch debranching enzyme were distinctly elevated in gif2 grains. In addition, the level of nucleotide diversity of the GIF2 locus was extremely low in both cultivated and wild rice. All of these results suggest that GIF2 plays important roles in the regulation of grain filling and starch biosynthesis during caryopsis development, and that it has been preserved during selection throughout domestication of modern rice.

关键词： GIF GRAIN INCOMPLETE FILlinG 2 regulates grain filling and starch synthesis during rice caryopsis development Figure AGPS RNAi

Review: Psychological Problems of Pregnant Women and Progress of Psychological Guidance in Recurrent Miscarriage

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Health 2021年第7期13卷 729-735页

作者： haiyan lin Chenyun Xu The Fourth People’s Hospital of Haikou Haikou China Hainan General Hospital Haikou China

In order to understand the psychological problems of pregnant women, this article explores the psychological problems and the sources of psychological stress of pregnant women who have a history of recurrent miscarriage after pregnancy. The analysis shows that the psychological stress of pregnant women may be one of the reasons for recurrent miscarriage. Strengthening mental health education during pregnancy can avoid the stimulation of bad emotions and environment, prevent the spontaneous abortion caused by psychological reasons, and improve the quality of pregnancy. It is particularly important to grasp the mental state of pregnant women with recurrent abortion timely and carry out corresponding psychological counseling and psychological nursing.

关键词： Pregnant Women Recurrent Abortion Psychological Factors Psychological Guidance