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Water Masses in the South China Sea and Water Exchange between the Pacific and the South China Sea

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Journal of Ocean University of Qingdao 2002年第1期1卷 19-24页

作者： LIFengqi lilei WANGXiuqin LIUChangle CollegeofEnvironmentalScienceandEngineering OceanUniversityofQingdaoQingdao266003P.R.China CollegeofPhysicalandEnvironmentalOceanography OceanUniversityofQingdaoQingdao266003P.R.China

Water masses in the South China Sea (SCS) were identified and analyzed with the data collected in the summer and winter of 1998. The distributions of temperature and salinity near the Bashi Channel (the Luzon Strait) were analyzed by using the data obtained in July and December of 1997. Based on the results from the data collected in the winter of 1998, waters in the open sea areas of the SCS were divided into six water masses: the Surface Water Mass of the SCS (S), the Subsurface Water Mass of the SCS (U), the Subsurface-Intermediate Water Mass of the SCS (UI),the Intermediate Water Mass of the SCS (I), the Deep Water Mass of the SCS (D) and the Bottom Water Mass of the SCS(B). For the summer of 1998, the Kuroshio Surface Water Mass (KS) and the Kuroshio Subsurface Water Mass (KU) were also identified in the SCS. But no Kuroshio water was found to pass the 119.5°E meridian and enter the SCS in the time of winter observations. The Sulu Sea Water (SSW) intruded into the SCS through the Mindoro Channel between 50-75 m in the summer of 1998. However, the data obtained in the summer and winter of 1997 indicated that water from the Pacific had entered the SCS through the nor-thern part of the Luzon Strait in these seasons, but water from the SCS had entered the Pacific through the southern part of the Strait. These phenomena might correlate with the 1998 El-Nio event.

关键词： the South China Sea water mass Luzon Strait (Bashi Channel) Mindoro Channel water exchange

刚地弓形虫感染对小鼠脑组织转录本m^(6)A甲基化修饰的影响

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中国寄生虫学与寄生虫病杂志 2024年第1期42卷 27-35,47页

作者：解晓曼孙航代莉莎朱文菊王利磊谢环环董宏杰张俊梅王琦周贝贝赵桂华徐超尹昆山东省寄生虫病防治研究所山东第一医科大学(山东省医学科学院)济宁272033

目的分析刚地弓形虫感染对小鼠脑组织转录本的N6-甲基腺苷(m6A)甲基化修饰水平的影响。方法20只雌性C57BL/6J小鼠随机分为TgCtwh6感染组(7只)、LHG感染组(7只)和对照组(TgCtwh6感染组、LHG感染组的对照各3只)。TgCtwh6感染组、LHG感染... 详细信息

目的分析刚地弓形虫感染对小鼠脑组织转录本的N6-甲基腺苷(m6A)甲基化修饰水平的影响。方法20只雌性C57BL/6J小鼠随机分为TgCtwh6感染组(7只)、LHG感染组(7只)和对照组(TgCtwh6感染组、LHG感染组的对照各3只)。TgCtwh6感染组、LHG感染组分别灌胃接种中国Ⅰ型wh6株(TgCtwh6)和中国Ⅲ型LHG株刚地弓形虫感染小鼠脑组织悬液0.2ml(20个包囊/鼠),对照组灌胃等量的生理盐水。分别在接种后15、30和45 d,用抽签法随机抽取感染组小鼠各1只,麻醉后处死,取脑组织,于显微镜下分别记录大脑皮层区、海马区和嗅球区的包囊数。感染后45 d每组分别取3只小鼠的全脑组织,提取总RNA,制备基因组文库,进行转录组测序,筛选差异甲基化位点(DML),统计感染组和对照组的mRNA的差异m6A甲基化位点及其所在转录本;对甲基化位点所在转录本进行基因本体功能注释(GO)分析,京都基因与基因组百科全书(KEGG)富集分析,对甲基化差异转录本进行基因集富集分析(GSEA)。选取甲基转移酶样3(METTL3)、肥胖相关蛋白(FTO)和YTH结构域3(YTHDF3)等3种m6A甲基化修饰蛋白基因,以甘油醛-3-磷酸脱氢酶基因为内参,实时荧光定量逆转录PCR(qRT-PCR)检测其相对转录水平。结果感染组小鼠均感染成功。感染后45 d,大脑皮层、海马和嗅球的包囊数分别为(5676±10)、(4773±9)、(243±10)个。感染组和对照组共检出760650个甲基化位点,其中GGACA、GGACC、GGACT、ACGAT等4种m6A模体的甲基化水平分别占16.8%(127923/760650)、4.6%(35164/760650)、3.8%(28983/760650)和0.4%(3122/760650);共检出高甲基化位点所在转录本127016条,其中GGACA、GGACC、GGACT和ACGAT所在的转录本分别为71727、27754、24556和2979条。感染组与对照组小鼠脑组织转录组中ACGAT、GGACA、GGACC、GGACT等4种m6A模体的DML位点总数为9233,其中高甲基化位点4832个,低甲基化位点4851个。GO分析结果显示,DML所在转录本显著富集于生物过程中的细胞过程、细胞组分中的细胞和分子功能中的结合等功能。DML所在转录本的KEGG富集分析结果显示,分子间相互作用网络主要富集在溶酶体、剪接体和多巴胺能突触信号通路等途径中。对生物过程的GO富集分析结果显示,DML所在的转录本中,生物过程相关的部分转录本主要富集于蛋白糖基化、神经元凋亡负调控、蛋白质的入核转运等过程。GSEA分析结果显示,对照组和两个感染组的组间差异甲基化高分富集于成纤维细胞增殖负调控通路和Hippo信号通路相关的转录本子集中,其中筛选出ENSMUST00000105393、ENSMUST00000006523、ENSMUST00000055261和ENSMUST00000038658等4个关键转录本,分别编码共刺激因子配体(ICOSL)、富含半胱氨酸肠蛋白1(CRIP1)、MOB激酶激活剂1A201(MOB1A201)和MOB1A202,与对照组相比,TgCtwh6感染组、LHG感染组的这4个基因表达均上调。qRT-PCR结果显示,TgCtwh6感染组、LHG感染组小鼠脑组织mettl3 mRNA相对表达水平分别为5.47±1.09、1.63±0.06,与对照组(1.01±0.11)比较差异均有统计学意义(t=4.05、5.03,均P<0.05);ythdf3 mRNA相对表达水平分别为3.57±0.08、1.80±0.25,与对照组(1.01±0.11)比较差异均有统计学意义(t=18.95、2.85,均P<0.05);fto mRNA相对表达水平分别为0.41±0.04、0.60±0.12,与对照组(1.00±0.06)比较差异有统计学意义(t=7.67、2.99,均P<0.05);qRT-PCR结果与转录组测序获得的转录趋势一致。结论弓形虫慢性感染可导致小鼠脑组织转录本m6A甲基化修饰水平升高,并可能通过对icosl、crip1和mob1a等关键差异转录本的修饰调控,造成宿主脑组织内与精神行为相关的生物学进程和信号通路的改变。

关键词：刚地弓形虫 m6A修饰转录本脑包囊纳米孔三代测序

盐酸安非他酮联合盐酸舍曲林对青少年抑郁症疲劳感的治疗效果

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中国健康心理学杂志 2024年第6期32卷 801-806页

作者：杜晖戴立磊陈佳悦何欣芙张伟舒婧阳广杰湖北省荆门市人民医院临床心理科 448000 上海交通大学医学院附属精神卫生中心

目的:探讨盐酸安非他酮联合盐酸舍曲林对青少年抑郁症疲劳感的治疗效果。方法:于2021年6月至2023年5月收集100例青少年抑郁症患者进行研究,随机分为研究组与对照组各50例,两组患者均采用盐酸舍曲林治疗,研究组在盐酸舍曲林基础上联合盐... 详细信息

目的:探讨盐酸安非他酮联合盐酸舍曲林对青少年抑郁症疲劳感的治疗效果。方法:于2021年6月至2023年5月收集100例青少年抑郁症患者进行研究,随机分为研究组与对照组各50例,两组患者均采用盐酸舍曲林治疗,研究组在盐酸舍曲林基础上联合盐酸安非他酮治疗,治疗前及治疗2周、4周后采用患者健康问卷抑郁症状群量表(PHQ-9)、广泛性焦虑障碍量表(GAD-7)、疲劳量表(FS-14)进行心理评估,明确治疗效果。结果:①两组患者治疗前PHQ-9、GAD-7评分比较差异均无统计学意义(P>0.05),治疗2周、4周后研究组的PHQ-9评分比对照组降低的更明显,重复测量方差分析显示治疗后两组PHQ-9评分的时间效应、组间效应及交互效应均显著(F_(时间)=447.956,F_(组间)=4.305,F_(交互)=30.358;P0.05);②两组患者治疗前FS-14评分比较差异均无统计学意义(P>0.05),治疗2周、4周后研究组的FS-14评分比对照组降低的更明显,重复测量方差分析显示治疗后两组FS-14评分的时间效应、组间效应及交互效应均显著(F_(时间)=784.133,F_(组间)=149.191,F_(交互)=66.486;P0.05);治疗2周后研究组每日平均卧床时间明显短于对照组,两组比较差异有显著的统计学意义(P0.05);重复测量方差分析显示治疗后两组每日平均卧床时间的时间效应、组间效应及交互效应均显著(F_(时间)=319.977,F_(组间)=5.965,F_(交互)=6.929;P<0.05);④研究组的坐立不安、激越的发生率明显高于对照组,两组比较差异有统计学意义(χ^(2)=6.462,4.914;P<0.05)。结论:盐酸安非他酮联合盐酸舍曲林可以改善青少年抑郁症抑郁情绪及疲乏感,尽早缩短卧床时间,有利于疾病治疗,但需监测坐立不安、激越的不良反应发生率。

关键词：青少年抑郁症盐酸安非他酮盐酸舍曲林疲乏感

RNA structure determination:From 2D to 3D

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Fundamental Research 2023年第5期3卷 727-737页

作者： Jie Deng Xianyang Fang Lin Huang Shanshan Li lilei Xu Keqiong Ye Jinsong Zhang Kaiming Zhang Qiangfeng Cliff Zhang Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Guangdong-Hong Kong Joint Laboratory for RNA MedicineSun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Beijing Frontier Research Center for Biological Structure Center for Synthetic and Systems BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Key Laboratory of RNA Biology CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China MOE Key Laboratory for Cellular Dynamics and Center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China University of Chinese Academy of Sciences Beijing 100049China MOE Key Laboratory of Bioinformatics Beijing Advanced Innovation Center for Structural Biology&Frontier Research Center for Biological StructureCenter for Synthetic and Systems BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua-Peking Center for Life Sciences Beijing 100084China

RNA molecules serve a wide range of functions that are closely linked to their *** basic structural units of RNA consist of single-and double-stranded *** order to carry out advanced functions such as catalysis and ligand binding,certain types of RNAs can adopt higher-order *** analysis of RNA structures has progressed alongside advancements in structural biology techniques,but it comes with its own set of challenges and corresponding *** this review,we will discuss recent advances in RNA structure analysis techniques,including structural probing methods,X-ray crystallography,nuclear magnetic resonance,cryo-electron microscopy,and small-angle X-ray ***,a combination of multiple techniques is employed for the integrated analysis of RNA *** also survey important RNA structures that have been recently determined using various techniques.

关键词： RNA structure RNA structure probing X-ray crystallography Nuclear magnetic resonance spectroscopy Cryo-electron microscopy Small angle X-ray scattering

Tidal Analysis of High and Low Water Data

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Journal of Ocean University of China 2004年第1期3卷 10-16页

作者： LIPeiliang lilei ZUOJuncheng ZHAOWei CHENZongyong DepartmentofOceanography OceanUniversityofChinaQingdao266003P.R.China InstituteofOceanology ChineseAcademyofScienceQingdao266071P.R.China

The harmonic analysis method based on high and low water levels is discussed in this paper. In order to make full use of the information of high and low water observations (the time derivative of water level at the observation time is zero), the weight coefficient, w, is introduced to control the importance of the part related to this information in the error formula. The major diurnal constituents, O 1 and K 1, and semi diurnal constituents, N 2, M 2 and S 2 are selected directly from the monthly data analysis, and some other important constituents, P 1, ν 2 and K 2, are included as the inferred constituents. The obtained harmonic constants of the major constituents are very close to those obtained from the analysis of hourly data, and this shows that high and low water data can be used to extract tidal constants with high accuracy. The analysis result also shows that the inference and the weighting coefficient are important in the high and low water data analysis, and it is suggested that w ≥1 should be taken in monthly high and low water data analysis. This analysis method can be used directly to analyze altimetric data with w =0.

关键词： tidal analysis high and low water data altimetric data

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Yb,Er:YAG中能量上转换过程研究

光学学报 2000年第9期20卷 1277-1282页

作者：黄莉蕾洪治中国计量学院光电子研究所杭州310034 浙江大学光学工程系杭州310027

计算了 Yb,Er:YAG晶体中 Yb3 + 和 Er3 + 离子之间交叉弛豫几率 ,分析了 Yb,Er:YAG晶体中能量上转换过程。通过求解跃迁速率方程 ,得出了 Yb3 + 和 Er3 +

关键词：交叉弛豫能量上转换激光晶体掺杂

CDIO模式下临床医学专业课程思政实践路径研究

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中国毕业后医学教育 2024年第8期8卷 608-611页

作者：周丽平王晓菲王悦怡吴雨晴江洪余锂镭武汉大学人民医院心血管内科湖北武汉430060

目的探索运用CDIO模式对提升临床医学专业课程思政实践路径。方法以武汉大学第一临床学院和武汉大学基础医学院的临床医学课程的任课老师和学员为访谈对象,采用半结构化访谈法对访谈资料进行整理和分析。结果 CDIO模式下提升临床医学... 详细信息

目的探索运用CDIO模式对提升临床医学专业课程思政实践路径。方法以武汉大学第一临床学院和武汉大学基础医学院的临床医学课程的任课老师和学员为访谈对象,采用半结构化访谈法对访谈资料进行整理和分析。结果 CDIO模式下提升临床医学专业课程思政实践路径,需完善课程思政构思,提升师生对课程思政的认识;改进专业课程设计,挖掘思政教育元素;丰富课程思政实施过程,开展多样化教学方式;优化课程思政运行效果,构建动态客观的评价体系。结论 CDIO模式下提升临床医学专业课程思政实践路径,有助于提升师生对课程思政的认识,开展多样化课程思政教学方式,有助于学员树立正确的职业信仰,提升医学综合素质,更好地促进学员全面发展。

关键词：临床医学课程思政 CDIO模式实践路径评价体系

Imbibitions, energy test and accelerated ageing in primed and non-primed seeds of Peltophorum dubium

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Journal of Forestry Research 2005年第2期16卷 113-116页

作者： lilei-hong ZHANGWan-li ZUYuan-gang SONIAPerez KeyLaboratoryofForestPlantEcology NortheastForestryUniversityHarbin150040China EastChinaNormalUniversity＇Shanghai200062 China FederalUniversityofSaoCarlos SaoCarlos13565-905Brazil

Peltophorum dubium seeds were set to imbibe with four treatments, soaked with solution Captan 0.2% under 10and 27℃,PEG 6000 -1.0 MPa under 10 and 27℃. For each treatment there were four replicates with 40 seeds incubated in 9-cm Petri dishes with double filter paper moistened with testing solution. The imbibition curves showed that the final weight increase were from 70% to 150% in the treatments when imbibition entered a lag phase. Seeds were tested for effects on germination of five treatments: control group (nonprimed), primed with PEG6000 -1.0 MPa at 10 and 27℃, primed with Captan 0.2% at 10 and 27℃. For each treatment, there were three sub-treatments: seeds were soaked in distilled water for 12, 24 and 36h before the energy test. Germination percentages of nonprimed seeds and primed in PEG 27℃ soaked in distilled water during 12 h were the highest, reaching 100%. The lowest germination percentage occurred primed seeds with PEG6000 27℃ and soaked in distilled water during 36 h, which was only 52%. Germination mean time of primed seeds in PEG at 10℃, soaked 24 h was 1.08 days, mean time of primed seeds in PEG at 27℃ soaked 12 h was 2.42 days. Accelerated ageing results showed low or no germination after ageing 72 h. Control group had a higher germination percentage and seeds were more resistant to deterioration than those in primed groups, both in Petri dish (27℃) and vermiculate (room temperature).

关键词： peltophorum dubium seeds Imbibition priming germination ageing

神经内镜下微血管减压术治疗面肌痉挛患者的效果及对术后康复、复发的影响

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中外医学研究 2024年第16期22卷 34-37页

作者：邓明亮张辉翟红洁韩冠达李磊董小辉娄飞云蚌埠医科大学第一附属医院安徽蚌埠233000

目的:分析神经内镜下微血管减压术治疗面肌痉挛患者的效果,探讨其对术后康复及复发的影响。方法:选取2020年1月—2023年1月蚌埠医科大学第一附属医院神经外科收治的80例面肌痉挛患者作为研究对象,随机分为对照组和观察组,各40例。对照... 详细信息

目的:分析神经内镜下微血管减压术治疗面肌痉挛患者的效果,探讨其对术后康复及复发的影响。方法:选取2020年1月—2023年1月蚌埠医科大学第一附属医院神经外科收治的80例面肌痉挛患者作为研究对象,随机分为对照组和观察组,各40例。对照组采取常规微血管减压术治疗,观察组采取神经内镜下微血管减压术治疗,比较两组手术指标、治疗效果、术后并发症、复发情况等。结果:观察组手术时间、住院时间短于对照组,差异有统计学意义(P<0.05);观察组治疗总有效率高于对照组,而术后并发症发生率及术后1年复发率低于对照组,差异有统计学意义(P<0.05)。结论:常规微血管减压术、神经内镜下微血管减压术都是治疗面肌痉挛的方法,而神经内镜下微血管减压术是在微血管减压术的基础上利用神经内镜辅助观察和操作,进一步提高了手术视野清晰度,不仅有利于缩短手术时间,还能减少并发症风险,从而提高手术效果和术后康复速度,有效缩短住院时间,随访1年复发率也明显更低。

关键词：面肌痉挛显微手术微血管减压术神经内镜复发控制治疗效果