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Expression profiles of penaeidin from Fenneropenaeus chinensis in response to WSSV and vibrio infection by real-time PCR

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Acta Oceanologica Sinica 2005年第2期24卷 131-140页

作者： DONGBo LIUFengsong XIANGJianhai lifuhua GAOHongwei ExperimentalMarineBiologylaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China／／GraduateSchoolChineseAcademyofSciencesBeijing100039China ExperimentalMarineBiologylaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China QingdaoInspectionandQuarantineBureau Qingdao266101China

Penaeidin from Chinese shrimp (Fenneropenaeus chinensis) has proved to be one of the most important antimicrobial peptides in the bodies of animals. The relative quantitative real-time PCR method is developed to study through time, the mRNA expression profile of penaeidin in the muscle and haemocyte tissue of Chinese shrimp infected with vibrio (Vibrio anguillarum) and WSSV (white spot syndrome virus). Research results showed that the same pathogens infection experiments produced similar gene expression profile in different tissues while different expression profiles appeared in the same tissues infected by different exterior pathogens. In vibrio infection experiments, a 'U' like expression profile resulted. Expression levels of penaeidin increased and surpassed the non-stimulated level, indicating that penaeidin from Chinese shrimp has noticeable antimicrobial activities. In WSSV infection experiments, the expression profile appeared as an inverse 'U' with the expression of penaeidin gradually decreasing to below baseline level after 24 h. The expression of antimicrobial peptides gene in mRNA level in response to virus infection in shrimp showed that international mechanisms of virus to haemocytes and microbial to haemocytes are completely different. Decline of penaeidins expression levels may be due to haemocytes being destroyed by WSSV or that the virus can inhibit the expression of penaeidins by yet undiscovered modes. The expression profiles of penaeidin in response to exterior pathogen and the difference of expression profiles between vibrio and WSSV infection provided some clues to further understanding the complex innate immune mechanism in shrimp.

关键词： penaeidin Fenneropenaeus chinensis expression profile WSSV Vibrio anguillarum real-time PCR

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Cloning,sequencing and expression analysis of cDNA encoding a constitutive heat shock protein 70 (HSC70) in Fenneropenaeus chinensis

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Chinese Science Bulletin 2004年第22期49卷 2385-2393页

作者： JIAOChuanzhen WANGZaizhao lifuhua ZHANGChengsong XlANGJianhai InstituteofOceanology ChineseAcademyofSciencesQingdao266071China//DepartmentofBiologyDezhouCollegeDezhou253023China//GraduateSchoolofChineseAcademyofSciencesBeijing100039China InstituteofOceanology ChineseAcademyofSciencesQingdao266071China

The cDNA encoding hsc70 of Chinese shrimp Fenneropenaeus chinensis was cloned from hepatopancreas by RT-PCR based on its EST sequence. The full length cDNA of 2090 bp contained an open reading frame of 1956 nucleotides and partial 5′- and 3′-untranslated region(5′- and 3′-UTR). PCR amplification and sequencing analysis showed the existence of introns in the region of 1-547 bp, but they did not exist in the region of 548-2090 bp of hsc70 cDNA. When the deduced 652 amino acid sequence of HSC70 was compared with the members of HSP70 family from other organisms, the results showed 85.9% similarity with HSC71 from Oncorhynchus mykiss and HSC70 from Homo sapiens. It also exhibited 85.8% similarity with HSP70 from Mus musculu and 85.4% with HSC70 from Manduca sexta. Ex- pression analysis showed that hsc70 mRNA was espressed constitutively in hepatopancreas, muscle, eyestalks, haemo- cytes, heart, ovary, intestine and gills in Fenneropenaeus chinensis. No difference could be detected on hsc70 mRNA level in muscle between heat-shocked and control animals.

关键词：无性繁殖 cDNA 表达方式编码热震动蛋白质70 HSC70 虾

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Studies on hybridization effects of different geographic populations of Chlamys farreri Ⅱ.The medium-term growth and development of Chlamys farreri populations from China and Russia and their reciprocal crosses

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Acta Oceanologica Sinica 2003年第2期22卷 255-264页

作者： LiuXiaolin ChangYaqing XiangJianhai lifuhua LiuXianjie BrovkinaElenaPavlovna CollegeofAnimalScienceandTechnology NorthwestSci-TechUniversityofAgricultureandForestryYangling712100China KeyLabofMarineEcologyinAquacultureofMinistryofAgriculture DalianFisheriesUniversityDalian116023China ExperimentalMarineBiologyLaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China KeyLabofMarineEcologyinAquacultureofMinistryofAgricultureofMinistryofAgriculture DalianFisheriesUniversityDalian116023China Far-EastStateTechnicalFisheriesUniversity Vladivostok690000Russia

Based on the research of juvenile (2, 3, 4 months) growth and survival of three populations of two different geographic areas in Chlamys farreri from Russian and China and their F1 hybrids derived from Chinese cultural population (CC) (?) × Russian population (RW) (?) , Chinese wild population (CW) (?) × Russian population (RW) (?), Russian population (RW) (?) × Chinese wild population (CW) (?) , the study of the medium-term (6, 8, 10, 12 months) growth and development of Chlamys farreri was carried out. The four determined results indicated that there existed different extent heterosis (3% -52%) for the growth in three types of F1 hybrids, and the offspring derived from CC(?) ×R(?) had a stronger heterosis among the crosses at the medium-term; the uptrend among traits are wet weight >shell width>shell length> shell height, Chinese cultural population could be recognized as excellent parent, and seasonal variations influence very much on the daily increment and growth rate of each trait of Chlamys farreri and it is only able to survive and could barely grow in winter (6-8 months), but grows fast in temperate season (10-12 months).

关键词： Chlamys farreri population hybridization medium-term growth and development heterosis

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