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Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor

science china(life sciences) 2009年第10期52卷 911-921页

作者： liao zhiyong1, chen xiaoli2 & wu mingjiang1 1 college of life and environmental science, wenzhou university, wenzhou 325035, china 2 Capital Institute of Pediatrics, Beijing 100020, china 1. college of life and environmental science Wenzhou University Wenzhou 325035 China 2. Capital Institute of Pediatrics Beijing 100020 China

A full length cDNA encoding the growth hormone receptor (GHR) of Chinese sturgeon was cloned in order to investigate the mechanism of growth hormone in regulating the growth of Chinese sturgeon. The open reading frame of the cloned Chinese sturgeon growth hormone receptor (csGHR) cDNA encodes a trans-membrane protein of 611 amino acids containing all the characteristic motifs of GHR. By sequence alignment, substitutions of amino acid residues highly conserved in other species were identified. Using the CHO cell culture system, the function of csGHR and the biological significance of the amino acid substitution in csGHR were examined. The promoter of serine protease inhibitor 2.1 (Spi2.1) was trans-activated upon stimulation of seabream GH (sbGH) in the csGHR-expressing CHO cells. Furthermore, CHO cells stably expressing csGHR were stimulated to proliferate by sbGH. In agreement with our previous report, Chinese sturgeon growth hormone-binding protein (csGHBP) was detected in the culture medium of CHO cells stably expressing csGHR. Mutation of Asp residue in the ligand binding motif in csGHR to Glu significantly enhanced csGHR's biological function, whereas mutation of Asp residue to Ala decreased its biological function. The results demonstrated that the cloned csGHR was of full biological function and the csGHBP could be generated through proteolysis of csGHR. These findings might provide new insights into thoroughly understanding the regulatory mechanism of Chinese sturgeon growth.

关键词： Chinese sturgeon GHR GHBP mutation cDNA cloning

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手性柱芳烃及其自组装研究进展

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中国科学：化学 2023年第12期53卷 2357-2366页

作者：郑宸吴旋廖志勇温州大学生命与环境科学学院温州325000 扬州大学化学化工学院扬州225000

超分子手性是一种在自组装过程中由于对称性缺失而形成的手性现象,近年来受到了广泛的关注.其研究的核心问题是如何设计组装基元,并控制其自组装行为以得到具有特定功能特定形貌的手性组装体.大环化合物由于丰富的主客体化学性质及特殊... 详细信息

超分子手性是一种在自组装过程中由于对称性缺失而形成的手性现象,近年来受到了广泛的关注.其研究的核心问题是如何设计组装基元,并控制其自组装行为以得到具有特定功能特定形貌的手性组装体.大环化合物由于丰富的主客体化学性质及特殊的立体结构,可通过手性传递或者官能团修饰的方法获得手性,因而在手性超分子自组装方面有着广泛的应用.本综述针对近年来基于柱芳烃的手性体系进行总结,首先对手性柱芳烃的构筑进行概述,基于柱芳烃自身的主客体化学性质及其结构特性,手性柱芳烃可通过手性传递、结构修饰及空间限域等策略获得;然后对柱芳烃手性自组装体系的构筑和应用进行了总结,论述了其在手性存储、手性发光、手性识别等方面的应用;最后总结和展望了柱芳烃手性自组装体系的前景.

关键词：超分子手性手性柱芳烃手性传递面手性手性自组装

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Bevacizumab biosimilar LY01008 compared with bevacizumab(Avastin)as first-line treatment for Chinese patients with unresectable,metastatic,or recurrent non-squamous non-small-cell lung cancer:A multicenter,randomized,double-blinded,phase Ⅲ trial

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Cancer Communications 2021年第9期41卷 889-903页

作者： Yuankai Shi Kaijian Lei Yuming Jia Bingqiang Ni zhiyong He Minghong Bi Xicheng Wang Jianhua Shi Ming Zhou Qian Sun Guolei Wang Dongji chen Yongqian Shu Lianke Liu Zhongliang Guo Yong Liu Junquan Yang Ke Wang Ke Xiao Linwu Tienan Yi Debin Sun Mafei Kang Tianjiang Ma Yimin Mao Jinsheng Shi Tiegang Tang Yan Wang Puyuan Xing Dongqing Lv Wangjun liao Zhiguo Luo Bin Wang Xiaohong wu xiaoli Zhu Shuhua Han Qisen Guo Rongyu Liu Zhiwei Lu Jianyong Zhang Jian Fang Changlu Hu Yinghua Ji Guolong Liu Hong Lu Dedong wu Junhong Zhang Shuyang Zhu Zheng Liu Wensheng Qiu Feng Ye Yan Yu Yanqiu Zhao Qinhong Zheng Jun chen Zhanyu Pan Yiping Zhang Wenjuan Lian Bo Jiang Bo Qiu Guojun Zhang Hua Zhang Yanju chen Yuan chen Hongbing Duan Manxiang Li Shengming Liu Lijun Ma Hongming Pan Xia Yuan Xueli Yuan Yulong Zheng Emei Gao Li Zhao Shumin Wang Can wu Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing Key Laboratory of Clinical Study on Anticancer Molecular Targeted DrugsBeijing 100021P.R.China Department of Oncology Yibin Second People’s HospitalYibinSichuan 644000P.R.China Department of Oncology Liuzhou People’s HospitalLiuzhouGuangxi 545006P.R.China Department of Thoracic Oncology Fujian Provincial Cancer HospitalFuzhouFujian 350014P.R.China Oncology Department the First Affiliated Hospital of Bengbu Medical CollegeBengbuAnhui 233030P.R.China Department of Oncology the First Affiliated Hospital of Guangdong Pharmaceutical UniversityGuangzhouGuangdong 510030P.R.China Department of Internal medicine Linyi Cancer HospitalLinyiShandong 276002P.R.China Department of Thoracic Surgery the Affiliated Cancer Hospital of Guangzhou Medical UniversityGuangzhouGuangdong 510095P.R.China Department of Internal Medicine-Oncology/Department of thoracic oncology Henan Chest HospitalZhengzhouHenan 450003P.R.China The Second Department of Radiotherapy Wuwei Cancer Hospital of Gansu ProvinceWuweiGansu 730000P.R.China Department of Oncology the People’s Hospital of Jiangsu ProvinceNanjingJiangsu 210000P.R.China Respiratory Medicine Department Shanghai East HospitalShanghai 200120P.R.China The Second Department of Internal Medicine-Oncology Xuzhou Central HospitalXuzhouJiangsu 221009P.R.China Oncology Department Tangshan People’s HospitalTangshanHebei 063001P.R.China Respiratory Medicine Department West China Hospital of Sichuan UniversityChengduSichuan 610041P.R.China Thoracic Surgery the Second Affiliated Hospital of Shantou University Medical CollegeShantouGuangdong 515051P.R.China Internal Medicine-Oncology Hunan Cancer HospitalChangshaHunan 410013P.R.China Department of Oncology Xiangyang Central HospitalXiangyangHubei 441021P.R.China Respiratory Department Lishui Central HospitalLishuiZ

Background:Previous studies have demonstrated the preclinical pharmacological and toxicological consistency,and clinical pharmacokinetic equivalence of bevacizumab biosimilar LY01008 with reference bevacizumab(Avastin).This randomized controlled trial aimed to compare the efficacy and safety of LY01008 with Avastin in first-line treatment of Chinese patients with advanced or recurrent non-squamous non-small cell lung cancer(NSCLC).Methods:StageⅢB-ⅣNSCLC patients with evaluable lesions,good physical status,and adequate organ functions from 67 centers across china were randomized in a ratio of 1:1 to receive LY01008 or Avastin 15 mg/kg intravenously in combination with paclitaxel/carboplatin(combined treatment)for 4-6 cycles,followed by maintenance monotherapy with LY01008 until disease progression,intolerable toxicity,or *** primary endpoint was objective response rate(ORR)in accordance with Response Evaluation Criteria in Solid Tumors(RECIST)version 1.1 confirmed by independent radiological review committees(IRRC).Secondary endpoints included disease control rate(DCR),duration of response(DoR),progression-free survival(PFS),overall survival(OS),and *** study was registered in Clinical ***(NCT03533127).Results:Between December 15^(th),2017,and May 15^(th),2019,a total of 649 patients were randomized to the LY01008(n=324)or Avastin(n=325)*** of September 25th,2019 for primary endpoint analysis,589 patients received ORR evaluation,with a median number of combined treatment cycles of 5(range 1-6)andmedian duration of treatment of 3.0(range 0.0-5.1)*** responseevaluable patients in the LY01008 and Avastin groups were 48.5% and 53.0%,*** stratified ORR ratio was 0.91(90%CI 0.80-1.04,within the prespecified equivalence margin of 0.75-1.33).Up to May 15^(th),2020,with a median follow-up of 13.6(range 0.8-28.4)months,no notable differences in DCR,median DoR,median PFS,median OS,and 1-year OS rate were observed between the LY010

关键词： anti-angiogenesis anti-VEGF monoclonal antibody avastin bevacizumab biosimilar nonsmall cell lung cancer LY01008 vascular endothelial growth factor

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钙调素基因(HcCaM7)及其蛋白乙酰化修饰参与红麻响应非生物胁迫的作用

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作物学报 2023年第2期49卷 402-413页

作者：黄震吴启境陈灿妮吴霞曹珊张辉岳娇胡亚丽罗登杰李赟廖长君李茹陈鹏广西大学农学院/广西高校植物遗传育种重点实验室广西南宁530004 广西大学生命科学与技术学院广西南宁530004 广西博世科环保科技股份有限公司广西南宁530007

钙调素是一类钙依赖性调节蛋白,参与植物的生长发育、抗逆胁迫等多种生物学过程。本课题组前期通过蛋白质乙酰化修饰组学研究发现,红麻钙调素蛋白7的乙酰化修饰参与了红麻花粉的发育调控。为研究其参与抗逆性的机制,本研究以红麻保持系... 详细信息

钙调素是一类钙依赖性调节蛋白,参与植物的生长发育、抗逆胁迫等多种生物学过程。本课题组前期通过蛋白质乙酰化修饰组学研究发现,红麻钙调素蛋白7的乙酰化修饰参与了红麻花粉的发育调控。为研究其参与抗逆性的机制,本研究以红麻保持系P3B双核期的花药为材料,使用PCR法克隆了钙调素基因HcCaM7,最大开放阅读框(open reading frame,ORF)为450 bp,其由149个氨基酸组成,编码相对分子质量16.85 kD的蛋白;亚细胞定位结果显示,HcCaM7蛋白的表达主要定位在细胞质和细胞膜中;利用病毒诱导的基因沉默技术沉默HcCaM7基因,导致红麻沉默植株的生长受到抑制;进一步在体外采用基因密码子扩展技术对发生乙酰化修饰氨基酸位点进行突变,成功获得具有体外乙酰化修饰位点的蛋白HcCaM7mut,并成功诱导表达了无乙酰化修饰的蛋白HcCaM7,结果表明HcCaM7蛋白发生乙酰化修饰后可以显著促进NADK(NAD激酶)活性;用点板法检测含有HcCaM7蛋白和HcCaM7mut蛋白的重组菌在盐(400 mmol L^(-1)和500 mmol L^(-1)NaCl)、干旱(400 mmol L^(-1)和600 mmol L^(-1)甘露醇)、重金属(30 mmol L^(-1)和50μmol L^(-1)CdCl2)及低温胁迫后(利用液氮反复冻融模拟)在LB固体培养基上的存活率发现,含有HcCaM7蛋白的重组菌存活率显著高于空载对照菌,而含有乙酰化修饰的HcCaM7mut蛋白的重组菌存活率进一步提升,表明HcCaM7蛋白能够提高大肠杆菌对非生物胁迫的耐受性,并且乙酰化修饰后效果更佳。因此,HcCaM7基因可以调控红麻生长发育和响应非生物胁迫,乙酰化修饰可以促进HcCaM7蛋白发挥作用。

关键词：红麻钙调素蛋白7 蛋白乙酰化修饰病毒诱导的基因沉默基因密码子扩展技术非生物胁迫

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β-氨基丁酸处理对采后草莓果实贮藏品质和内部还原势的影响

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食品与发酵工业 2018年第10期44卷 160-167页

作者：伍冬志费良航廖云霞陈偲汪开拓重庆三峡学院环境与化学工程学院重庆404000 重庆三峡学院生命科学与工程学院重庆404000 南京农业大学食品科技学院江苏南京210095

研究了10 mmol/Lβ-氨基丁酸(β-aminobutyric acid,BABA)、5 mmol/L 6-氨基烟酰胺(6-AN)以及两者复合处理对采后"丰香"草莓果实(Fragaria ananassa Duch. cv‘Fengxiang’)贮藏期间腐烂和品质的影响,并从还原势的角度探讨了BABA诱导果... 详细信息

研究了10 mmol/Lβ-氨基丁酸(β-aminobutyric acid,BABA)、5 mmol/L 6-氨基烟酰胺(6-AN)以及两者复合处理对采后"丰香"草莓果实(Fragaria ananassa Duch. cv‘Fengxiang’)贮藏期间腐烂和品质的影响,并从还原势的角度探讨了BABA诱导果实抗病性的相关机理。结果表明,经10 mmol/L BABA处理的草莓果实中TSS、TA、总花色苷和总酚含量以及DPPH自由基清除率和总还原力在20℃下贮藏5 d后均显著高于对照水平,而果实发病率则显著低于对照水平。BABA处理可促进草莓果实中还原性信号分子NO和SA的积累,并诱导磷酸戊糖途径关键酶G6PDH和6PGDH活性的上升,从而促使果实中还原性物质NADPH和GSH大量生成并降低氧化性产物NADP+和GSSG含量,同时果实Fa PR1、Fa Chi3、Faβglu和Fa PAL等PRs基因表达量也显著上升。单一6-AN或6-AN+BABA处理则显著降低了果实中NO和SA的含量,抑制G6PDH和6PGDH活性以及NADPH和GSH的积累,经单一6-AN或6-AN+BABA处理的果实中PRs基因表达量也显著下降。通过这些结果可推测,草莓果实抗病性的形成与还原势的增长密切相关; BABA处理可直接诱导还原性信号分子的生成,并通过调控PPP途径关键酶活性以积累还原性物质,全面增强草莓果实内部还原势,诱导一系列PRs基因的表达,最终抑制了贮藏期间腐烂的发生并改善了果实综合品质。

关键词： β-氨基丁酸草莓果实品质还原势磷酸戊糖途径诱导抗病性

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