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Field Emission from Nanostructured Carbon Films on Si Tips

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Chinese Physics Letters 2001年第8期18卷 1132-1134页

作者： WANG Wan-Lu liao Ke-jun HU Cheng-Guo FANG Liang Department of Applied Physics Chongqing UniversityChongqing 400044

Nanostructured carbon thin films on Si tips were prepared by hot filament chemical vapour deposition at different substrate *** Si tips and films were obtained under various deposition conditions in the same reaction *** was found that the field emission properties from graphite-like nanostructured carbon on Si tips were greatly improved as compared with those of nanodiamond films on Si tips.A turn-on field of 1.2 V·cm^(-1) was observed for high sp^(2) content thin films on Si *** analysis showed that the field emission enhancement effect was caused by the tip geometry,tunnel effect and sp^(2) content in the ***,the geometrical enhancement was greater than that of the tunnel and sp^(2) content effects.

关键词： films vapour field

Remote ischemia conditioning-an endogenous cardioprotective strategy from outside the heart

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Chinese Medical Journal 2011年第14期124卷 2209-2215页

作者： XIONG jun liao Xu XUE Fu-shan YUAN Yu-jing WANG Qiang LIU Jian-hua Department of Anesthesiology Plastic Surgery Hospital ChineseAcademy of Medical Sciences and Peking Union Medical CollegeBeijing 100144 China

Objective A general review was made of studies involving：（1） The experimental evidence of remote ischemic preconditioning （RIPC） and relative clinical studies, （2） The experimental and clinical evidences of remote ischemic postconditioning （RIPOC）, （3） The potential mechanistic pathways underlying their protective *** sources The data used in this review were mainly from manuscripts listed in PubMed that were published in English from 1986 to 2010. The search terms were ＂myocardial ischemia reperfusion injury＂, ＂ischemia preconditioning＂,＂ischemia postconditioning＂, ＂remote preconditioning＂ and ＂remote postconditioning＂.Study selection （1） Clinical and experimental evidence that both RIPC and RIPOC produce preservation of ischemia reperfusion injury （IRI） of myocardium and other organs, （2） Studies related to the potential mechanisms, by which remote ischemic conditioning protects myocardium against *** Both RIPC and RIOPC have been shown to attenuate myocardial IRI in laboratory animals. Also, their cardioprotective effects have appeared in some clinical studies. Except the external, the detailed internal mechanisms of remote ischemic conditioning have been generally described. Through these descriptions better protocols can be developed to provide improved cardioprotective *** Remote ischemic conditioning is an endogenous cardioprotective mechanism from outside the heart that protects against myocardial IRI and represents a general form of inter-organ protection. Remote ischemic conditioning may have an immense impact on clinical practice in the near future.

关键词： myocardial ischemic reperfusion injury ischemia preconditioning ischemia postconditioning remote preconditioning remote postconditioning

The Influence of Mesoscopic Characteristics of Mineral Powders Fillers on the Rutting Factor(G^(*)/sinδ)of Asphalt Mortar

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Journal of Wuhan University of Technology(Materials Science) 2023年第5期38卷 1118-1125页

作者： liao jun DENG Tao GONG Hongwei LI Zhen TANG Yongtao CHEN Kai SHI Lei QIAN Xiaolong DONG Qian College of Environment and Civil Engineering Chengdu University of TechnologyChengdu 610059China

This study aims to investigate the efiect of the mesoscopic characteristics of mineral powder fillers on the rutting resistance of asphalt *** and sieving tests were used to obtain the buton rock asphalt(BRA)ash with particle size smaller than 0.075 mm,which is consistent with that of the conventional mineral *** mesoscopic characteristics of BRA ash and conventional mineral powder were measured by SEM image analysis and the osmotic free pressure water *** structure models of structural and free asphalts in mortar were *** 70#matrix asphalt was used to prepare two kinds of asphalt mortar with BRA ash and conventional mineral powders *** rutting factor of the two asphalt mortars was tested by dynamic shear test(DSR).Test results show that the ash extracted from BRA has a similar mesoscopic classification with the conventional mineral ***,its fractal dimensions are larger,indicating the particles in BRA ash have more complex shapes and rougher surfaces,which is beneficial for forming structural asphalt and subsequently increasing the rutting factor(G*/sinδ),i e,improving the rutting resistance of the asphalt mortar.

关键词： asphalt mortar mineral powder fllers mesoscopic characteristic rutting factor(G*/sinδ)

Microsatellite-Based Genetic Differentiation and Phylogeny of Sheep Breeds in Mongolia Sheep Group of China

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Agricultural Sciences in China 2011年第7期10卷 1080-1087页

作者： SUN Wei CHANG Hong Musa Hussein Hassan liao Xin-jun CHU Ming-xing Kija James Animal Science and Technology College Yangzhou University Yangzhou 225009 P.R.China Faculty of Veterinary Science University of Nyala Nyala 155 Sudan Key Laboratory of Farm Animal Genetic Resources and Utilization Ministry of Agriculture/Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China Queensland Bioscience Precinct 306 Carmody Road St Lucia QLD 40723 Australia

In the present study we studied the genetic structure of five Chinese sheep populations of Hu sheep, Tong sheep, Small-tailed Han sheep, Tan sheep, and Wadi （WD） sheep using 15 microsatellite loci. The results showed that the FIT, FST, and FIS statistics computed for the complete dataset had the following values, 0.523±0.140, 0.363±0.131 and 0.263±0.092, respectively. All loci were significantly contributed to the genetic differentiation among population （P0.001）. There is no relationship between the scatter of pairwise FST geographical distance points as geographical distance increases between the five populations. Membership probabilities and genetic structure of sheep populations were estimated when K=2, the populations were classified into Hu, Tong, Han and WD, and Tan sheep group. However, when K=3, the populations were classified into Hu and Tong, Han and WD, and Tan sheep group. The findings supported the previous literatures that these populations are originated on different time stages from the primogenitor population and communicated genetically with each other by natural and artificial selection in different ecological environment.

关键词： genetic differentiation genetic phylogeny microsatellite DNA Mongolian sheep

Increased expression of 70 kD heat shock protein in cultured primary human keratinocytes induced by human papillomavirus 16 E6/E7 gene

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Chinese Medical Journal 2005年第24期118卷 2058-2062页

作者： liao Wen-jun FAN Ping-shen FU Meng FAN Xue-li LIU Yu-feng Department of Dermatology Xijing Hospital Fourth MilitaryMedical University Xi＇ an 710032 China

Background Heat shock protein 70 （HSP70） is expressed highly in epithelial tumours associated closely with human papillomavirus 16 （HPV16） infections. However, evidence about the direct relationship between HSP70 expression and HPVs infections are still lacking. In the present study, we examined the expression of HSP70 in keratinocytes introduced with HPV16 E6/E7 oncogenes. Methods Stable transfected cells were established by transfection of the plasmids pLXSN16E6/E7 into cultured primary keratinocytes and subsequently selected by plasmid specific selection antibiotic （G418） at the required concentration. The expression of HSP70 in pLXSN16E6/E7 transfected keratinocytes was determined by Western blot. The correlation of HSP70 expression and E6/E7 transfeetion was further confirmed by doubly labelled immunofluorescent staining. Results Compared to non-transfected keratinocytes, there was a significant trend for higher levels of HSP70 in pLXSN16E6/E7 transfected keratinocytes. Doubly labelled immunofluorescent staining experiment showed that the co-localization of HPV16 E6/E7 and HSP70 in transfeeted keratinoeytes was observed and increased expression of HSP70 was strongly associated with the transfection of HPV16 E6/E7. Conclusions Our studies demonstrated increased levels of HSP70 proteins in keratinocytes stably transfected by HPV16 E6/E7 oncogenes. It suggests that the expression of HSP70 is modulated by HPV16 E6/E7 proteins, which may be involved in HPV16 E6/E7 induced immortalization.

关键词： heat shock protein 70 keratinocytes human papillomavirus 16 E6/E7

地铁桩基钢套管施工对邻近铁路框架桥纵向变形影响分析

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现代城市轨道交通 2024年第8期 84-93页

作者：尹超刘维正盛越黎欢王锋廖军中建五局土木工程有限公司湖南长沙410004 中南大学土木工程学院湖南长沙410075 长沙市建设工程质量安全监督站湖南长沙410023

地铁桩基钢套管近距离施工会引起既有运营铁路框架桥的附加应力和变形,文章依托长沙地铁1号线北延线高架桥桩基近接既有铁路框架桥工程,基于Mindlin应力解和Pasternak地基梁,建立钢套管施工引起框架桥纵向变形的计算方法。结合实测数据... 详细信息

地铁桩基钢套管近距离施工会引起既有运营铁路框架桥的附加应力和变形,文章依托长沙地铁1号线北延线高架桥桩基近接既有铁路框架桥工程,基于Mindlin应力解和Pasternak地基梁,建立钢套管施工引起框架桥纵向变形的计算方法。结合实测数据对该方法的准确性进行验证,并分析桩径、地层弹性模量、相对净距及施工附加力对框架桥纵向变形的影响规律。结果表明:钢套管施工过程对周围土体的附加力主要包括土塞压力、套管侧径向压力和套管侧摩擦力,施工扰动主要源于钢套管的旋进过程;框架桥随钢套管施工深度的增加先隆起再逐渐沉降;套管侧摩擦力是框架桥变形的主要因素,径向压力对变形影响最小;纵向差异沉降与桩径成正比,与弹性模量和相对净距成反比;相对净距为框架桥变形的主要控制因素,在4倍桩径范围外时变形逐渐趋于零。工程中可从增大弹性模量和相对净距,减小土塞压力、套管摩阻力和桩径的角度控制框架桥变形,研究结果可为类似工程的设计施工提供依据。

关键词：地铁桩基钢套管近接施工纵向变形计算方法

基于(金刚烷)-1,2-二氧环丁烷结构的化学发光探针检测生物酶的研究进展

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黔南民族医专学报 2024年第2期37卷 127-132页

作者：杨政敏胡先运韩忠耀廖德君黔南民族医学高等专科学校贵州都匀558013

目前,在生理条件下基于(金刚烷)-1,2-二氧环丁烷结构的化学发光性能大幅提升,其化学发光方法检测各类小分子和生物大分子获得了广泛关注。利用该结构获得的化学发光探针具有无需外源激发光源、没有生物背景信号干扰、无光损伤、光漂白... 详细信息

目前,在生理条件下基于(金刚烷)-1,2-二氧环丁烷结构的化学发光性能大幅提升,其化学发光方法检测各类小分子和生物大分子获得了广泛关注。利用该结构获得的化学发光探针具有无需外源激发光源、没有生物背景信号干扰、无光损伤、光漂白和高灵敏度等优点。本文综述了近几年来基于(金刚烷)-1,2-二氧环丁烷结构的化学发光检测生物酶的研究进展,并讨论了该化学发光平台在生物检测方面潜在的挑战和前景。

关键词： (金刚烷)-1,2-二氧环丁烷化学发光生物酶

Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第11期20卷 891-900页

作者： Zhang-qing SONG Zhi-jun liao Ye-feng HU Zheng MA Andreas BECHTHOLD Xiao-ping YU Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine College of Life SciencesChina Jiliang UniversityHangzhou 310018China Institute for Pharmaceutical Sciences Pharmaceutical Biology and BiotechnologyUniversity of Freiburg79104 FreiburgGermany

An efficient genetic transformation system and suitable promoters are essential prerequisites for gene expression studies and genetic engineering in *** this study,firstly,a genetic transformation system based on intergeneric conjugation was developed in Streptomyces rimosus M527,a bacterial strain which exhibits strong antagonistic activity against a broad range of plant-pathogenic *** experimental parameters involved in this procedure were optimized,including the conjugative media,ratio of donor to recipient,heat shock temperature,and incubation time of mixed *** the optimal conditions,a maximal conjugation frequency of 3.05^10-5 per recipie nt was obtai *** ntly,based on the above developed and optimized tran sformati on system,the synthetic promoters SPL-21 and SPL-57,a native promoter potrB,and a constitutive promoter permE commonly used for gene expression in streptomycetes were selected and their activity was analyzed using gusA as a reporter gene in *** *** the four tested promoters,SPL-21 exhibited the strongest expression activity and gave rise to a 2.2-fold increase in p-glucuronidase(GUS)activity compared with the control promoter *** SPL-57 showed activity comparable to that of *** potrB,which showed the lowest activity,showed a 50%decrease in GUS activity compared with the control *** transformation system developed in this study and the tested promotors provide a basis for the further modification of *** M527.

关键词： Streptomyces rimosus M527 Intergeneric conjugation Promoter β-Glucuronidase(GUS)

Novel index for the prediction of significant liver fibrosis and cirrhosis in chronic hepatitis B patients in China

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World Journal of Gastroenterology 2022年第27期28卷 3503-3513页

作者： Min-jun liao jun Li Wei Dang Dong-Bo Chen Wan-Ying Qin Pu Chen Bi-Geng Zhao Li-Ying Ren Ting-Feng Xu Hong-Song Chen Wei-Jia liao Laboratory of Hepatobiliary and Pancreatic Surgery The Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology and Hepatology UnitNanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Genetics and Precision Medicine Laboratory The Affiliated Hospital of Guilin Medical UniversityGuilin 541001Guangxi Zhuang Autonomous RegionChina Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease Peking University People’s HospitalBeijing 100044China

BACKGROUND Noninvasive,practical,and convenient means of detection for the prediction of liver fibrosis and cirrhosis in China are greatly *** To develop a precise noninvasive test to stage liver fibrosis and *** With liver biopsy as the gold standard,we established a new index,[alkaline phosphatase(U/L)+gamma-glutamyl transpeptidase(U/L)/platelet(109/L)(AGPR)],to predict liver fibrosis and *** addition,we compared the area under the receiver operating characteristic curve(AUROC)of AGPR,gammaglutamyl transpeptidase to platelet ratio,aspartate transaminase to platelet ratio index,and FIB-4 and evaluated the accuracy of these routine laboratory indices in predicting liver fibrosis and *** Correlation analysis revealed a significant positive correlation between AGPR and liver fibrosis stage(P<0.001).In the training cohort,the AUROC of AGPR was 0.83(95%CI:0.78-0.87)for predicting fibrosis(≥F2),0.84(95%CI:0.79-0.88)for predicting extensive fibrosis(≥F3),and 0.87(95%CI:0.83-0.91)for predicting cirrhosis(F4).In the validation cohort,the AUROCs of AGPR to predict≥F2,≥F3 and F4 were 0.83(95%CI:0.77-0.88),0.83(95%CI:0.77-0.89),and 0.84(95%CI:0.78-0.89),*** The AGPR index should become a new,simple,accurate,and noninvasive marker to predict liver fibrosis and cirrhosis in chronic hepatitis B patients.

关键词： Liver Fibrosis Cirrhosis Prediction Novel noninvasive marker Chronic hepatitis B