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Journal of Integrative Plant Biology 2008年第8期50卷 1024-1030页

作者： Wan-jin liao Da-Yong Zhang Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering & Institute of Ecology Beijing Normal University Beijing 100875 China

Theory predicts that cosexual plants should adjust their resource investment in male and female functions according to their size if female and male fitness are differentially affected by size. However, few empirical studies have been carried out at both the flowering and fruiting stages to adequately address size-dependent sex allocation in cosexual plants. In this paper, we investigated resource investment between female and male reproduction, and their size-dependence in a perennial andromonoecious herb, Veratrum nigrum L. We sampled 192 flowering plants, estimated their standardized phenotypic gender, and assessed the resource investment in male and female functions in terms of absolute dry biomass. At the flowering stage, male investment increased with plant size more rapidly than female investment, and the standardized phenotypic femaleness （ranging from 0.267 to 0.776） was negatively correlated with plant size. By contrast, female biased allocation was found at the fruiting stage, although both flower biomass and fruit biomass were positively correlated with plant size. We propose that increased maleness with plant size at the flowering stage may represent an adaptive strategy for andromonoecious plants, because male flowers promote both male and female fertility by increasing pollinator attraction without aggravating pollen discounting.

关键词： andromonoecy phenotypic gender size-dependent sex allocation Veratrum nigrum

Underwater Oil Wettability on Nanostructured Superamphiphobic Surface Tuned by Trapped Air Layer Continuity

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Chemical Research in Chinese Universities 2014年第3期30卷 518-520页

作者： LI Shasha liao Mingyi jin Meihua Department of Materials Science and Engineering Dalian Maritime University Dalian 116026 P. R. China

When the superamphiphobic meshes are immersed in water, the rough structures on steel wires are filled with air. The nanostructured superamphiphobic surfaces were prepared on the stainless-steel mesh. By adjusting the mesh size of the surface, the continuity of trapped air layer on the superamphiphobic surface underwater could be controlled. Then the underwater oil-wetting behavior on the prepared superamphiphobic mesh was investigated. The oil droplet spread out on the superamphiphobic surface without mesh and exhibited an oil contact angle of about 0° under water. But the oil contact angle formed on the superamphiphobic mesh surfaces and extended with increasing mesh size. We thought the discontinuity of trapped air layer on the surface and the entry of water into interval between the steel wires should be responsible for these behaviors.

关键词： Underwater Wettability Superamphiphobic Air layer

An adaptive switching scheme for iterative computing in the cloud

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Frontiers of Computer Science 2014年第6期8卷 872-884页

作者： Yu ZHANG Xiaofei liao Hai jin Li LIN Feng LU Service Computing Technology and System Lab Cluster and Grid Computing Lab School of Computer Science and Technology Huazhong University of Science and Technology Wuhan 430074 China

Delta-based accumulative iterative computation （DAIC） model is currently proposed to support iterative algorithms in a synchronous or an asynchronous way. However, both the synchronous DAIC model and the asynchronous DAIC model only satisfy some given conditions, respectively, and perform poorly under other conditions either for high synchronization cost or for many redundant activations. As a result, the whole performance of both DAIC models suffers from the serious network jitter and load jitter caused by multi- tenancy in the cloud. In this paper, we develop a system, namely Hyblter, to guarantee the performance of iterative algorithms under different conditions. Through an adaptive execution model selection scheme, it can efficiently switch between synchronous and asynchronous DAIC model in order to be adapted to different conditions, always getting the best performance in the cloud. Experimental results show that our approach can improve the performance of current solutions up to 39.0%.

关键词： iterative algorithm computational skew communication skew cloud delta-based accumulative iterative computation

Pollen and Resource Limitation in Veratrum nigrum L. （Liliaceae）, an Andromonoecious Herb

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Journal of Integrative Plant Biology 2006年第12期48卷 1401-1408页

作者： Wan-jin liao Qing-Fa Song Da-Yong Zhang Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering and Institute of Ecology Beijing Normal University Beijing 100875 China

Pollen limitation and resource limitation were invoked to account for the pattern that flowering plants produce more flowers and ovules than fruits and seeds. This study aimed to determine their relative importance in Veratrum nigrum, a self-compatible, perennial, andromonoecious herb. In order to determine whether female production was limited by pollen grains on stigmas or by available resources, we performed supplemental hand pollination in three populations, male-flower-bud removal in three other populations, and emasculation of hermaphroditic flowers in still another population, resulting in a total of seven populations experimentally manipulated. Across the three populations, supplemental hand pollination did not significantly increase fruit set, seed number per fruit, and total seed production per individual, nor did emasculation of hermaphroditic flowers. Taken together, our results suggest that pollen grains deposited on stigmas were abundant enough to fertilize all the ovules. Male-flower-bud removal significantly increased the mean size of hermaphroditic flowers in all three populations. Female reproductive success was increased in one population, but not in the other two populations possibly due to heavy flower/seed predation. We concluded that the female reproductive success of V. nigrum was not limited by pollen grains but by available resources, which is consistent with Bateman＇s principle. Furthermore, the female reproduction increase of male-flower-bud removal individuals might suggest a trade-off between male and female sexual functions.

关键词： andromonoecy pollen limitation resource limitation trade-off Veratrum nigrum

Evaluation of Protocols Used in 2-D Electrophoresis for Proteome Analysis of Young Rice Caryopsis

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Genomics, Proteomics & Bioinformatics 2011年第6期9卷 229-237页

作者： Jiang-Lin liao Ying-jin Huang MOE Key Laboratory of Crop Physiological Ecology and Genetic Breeding Jiangxi Agricultural University Nanchang 330045 China.

In order to obtain a high-resolution electrophorogram of rice young panicle proteome, we evaluated various protocols commonly used in two-dimensional （2D） polyacrylamide gel electrophoresis （PAGE） of proteins, including gel staining protocol, pH range of immobilized pH gradient （IPG） strips and sample loading quantity. Results showed that a silver staining protocol using sensitized solution containing glacial acetic acid, sodium acetate and sodium thiosulfate （reported by Heukeshoven and Dernick in 1988） and a Coomassie Brilliant Blue staining method using solution containing G-250, ammonium sulfate and phosphoric acid （reported by Pink et al in 2010） demonstrated the superior staining effect. In addition, we also showed that higher resolution was achieved when IPG gel strip with pH range of 5-8 was used, compared to that with pH range of 4-7. Finally, the optimal loading quantity was determined as 130 ug using the 17 cm-long nonlinear IPG strip with pH 5-8 in combination with the silver nitrate staining protocol. The evaluated results would be helpful in proteome analysis of young rice caryopsis.

关键词： protocol 2-DE proteome rice caryopsis

Epilepsy and migrained-Are they comorbidity?

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Genes & Diseases 2018年第2期5卷 112-118页

作者： jin liao Xin Tian Hao Wang Zheng Xiao Neurology Department at Chongqing Medical University ChongqingChina Neurology Department at the First Affiliated Hospital of Chongqing Medical University No.1 Youyi StreetYuanjiagangYuzhong DistrictChongqingChina

Epilepsy and migraine often *** the clinical symptoms,they often have some signs of symptoms before onset;from the pathogenesis of epilepsy and migraine,both of them have a high degree of neuronal excitement and ion channel abnormalities;in terms of treatment,many antiepileptic drugs are work in *** of this indicates that they interact with each *** it is undeniable that there are interactions and relationships between them,and there are also some differences such as the different clinical episodes,the different ways of neuronal haperexcitability and the different drug treatment *** are they comorbidity?If we can better understand the correlation between seizures and migraines,then this will help develop better guidelines for clinical diagnosis and treatment.

关键词： Comorbidity CSD Epilepsy FHM Migraine

Determination of genetic relationships between evergreen azalea cultivars in China using AFLP markers

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第4期14卷 299-308页

作者： Hong ZHOU jin liao Yi-ping XIA Yuan-wen TENG Department of Horticulture College of Agriculture and BiotechnologyZhejiang University

Evergreen azaleas are among the most important ornamental shrubs in ***,there are probably over 300 cultivars preserved in different nurseries,but with little information available on the cultivar itself or relationships between *** fragment length polymorphism(AFLP) markers were employed to determine the genetic relationships between evergreen azalea cultivars in *** hundred and thirty genotypes collected from gardens and nurseries,including cultivars classified in the groups East,West,Hairy,and Summer,unknown cultivars,and close species,were analyzed using three primer pairs.A total of 408 polymorphic fragments were generated by AFLP reactions with an average of 136 fragments per primer *** average values of expected heterozygosity and Shannon's information index were 0.3395 and 0.5153,*** similarities were generated based on Dice coefficients,used to construct a neighbor joining tree,and bootstrapped for 100 replicates in Treecon *** coordinate analysis(PCO) was performed based on Dice distances using NTSYS-pc *** AFLP technique was useful for analyzing genetic diversity in evergreen *** analysis revealed that cultivars in the West and Summer groups were quite distinct from other groups in the four-group classification system and that the East and Hairy groups should be redefined.

关键词： Rhododendron spp. Tsutsusi Fluorescent label Dice coefficient Principal coordinate analysis(PCO) Neighbor joining tree

Acid deposition critical loads modeling for the simulation of sulfur exceedance and reduction in Guangdong, China

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Journal of Environmental Sciences 2009年第8期21卷 1108-1117页

作者： QIU Rongliang WANG Shizhong QIU Hao WANG Xuemei liao jin ZHANG Zhentian School of Environmental Science and Engineering Sun Yat-sen University Guangzhou 510275 China School of Earth and Atmospheric Sciences Georgia Institute of Technology Atlanta GA 30332 USA Guangdong Provincial Environmental Protection Bureau Guangzhou 510630 China

The current acid deposition critical loads in Guangdong, China were calculated using the PROFILE model with a 3 km × 3 km resolution. Calculations were carded out for critical loads of potential acidity, actual acidity, sulfur and nitrogen, with values in extents of 0-3.5, 0-14.0, 0-26.0 and 0-3.5 kmol/（***）, respectively. These values were comparable to previously reported results and reflected the influences of vegetation and soil characteristics on the soil acid buffering capacity. Simulations of SO2 emission and sulfur deposition in this study showed that sulfur deposition core areas mirrored SO2 emission centers. The prediction of sulfur deposition after 20% and 40% reduction of SO2 emission suggested that the reduction of area sources contributed greatly to the decrease of sulfur deposition. Thus, abatement of area source emissions could be the primary way to mitigate sulfur deposition in Guangdong to meet both the provincial and national regulations of air pollution control.

关键词： acid deposition critical loads modeling SO2 reduction sulfur deposition exceedance and reduction

An improved method for fluorescence analysis of dissolved organic matter in cave drip water

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Frontiers of Earth Science 2014年第4期8卷 595-598页

作者： Xiuli LI Chaoyong HU jin liao Liangliang BAO Qixi MAO State key Laboratory of Biogeology and Environmental Geology China University of Geosciences Wuhan 430074 China

An improved synchronous fluorimetric method for the determination of dissolved organic matter in cave drip water, by adding ascorbic acid, is described. The method is based on the redox reaction between ascorbic acid and the electron-withdrawing constituents in dissolved organic matter. The results show that adding ascorbic acid can quench the minor peaks, at 200-300 nm, but does not affect the intensity of the main peaks at 300-500nm. In addition, adding ascorbic acid can maintain relatively high and constant fuorescence intensity over a wide pH range （9-4）.

关键词： drip water synchronous fluorescence spectro-scopy ascorbic acid pH

The rhythmic expression of genes controlling flowering time in southern and northern populations of invasive Ambrosia artemisiifolia

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Journal of Plant Ecology 2015年第2期8卷 207-212页

作者： Xiao-Meng Li Da-Yong Zhang Wan-jin liao State Key Laboratory of Earth Surface Processes and Resource Ecology and MOE Key Laboratory for Biodiversity Science and Ecological Engineering College of Life SciencesBeijing Normal University19 Xinjiekouwai StreetHaidianBeijing 100875China

Aims Flowering time has been suggested to be an important adaptive trait during the dispersal of invasive species,and identifying the molecu-lar mechanisms underlying flowering time may provide insight into the local adaptation during the process of ***,we con-ducted a preliminary exploration on the genetic basis of the differ-entiation of flowering time in Ambrosia *** using relative real-time fluorescent quantitative polymerase chain reaction,we investigated the expression levels of eight flowering-related genes,including AP1,FT,SOC1,CRY2,FKF1,GI,CO2 and SPY,in leaves and flowers at different time points in individuals from northern beijing and southern Wuhan populations that exhibit significant differences in flowering times to identify any rhythmic changes in gene expression and their association with differential flowering *** Findings The differentiation of flowering time in the *** popula-tions was closely associated with five genes involved in flowering *** floral pathway integrators FT and SOC1 and floral meristem identity gene AP1 exhibited increased expression during *** photoreceptor CRY2 in the light-dependent path-way and the SPY gene in the gibberellin pathway displayed specific expression patterns over *** earlier-flowering beijing plants,CRY2 expression was lower and SPY expression was higher than in Wuhan *** expression patterns of these five genes sug-gest a molecular basis for the differentiation of flowering time in ***.

关键词： Ambrosia artemisiifolia flowering time adaptation flowering pathway gene expression