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Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling

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Genes & Diseases 2024年第2期11卷 964-977页

作者： Chengzhi Wu Hengyu Liu dongmei Zhong Xiaoming Yang Zhiheng liao Yuyu Chen Shun Zhang Deying Su Baolin Zhang Chuan Li Liru Tian Caixia Xu Peiqiang Su Guangdong Provincial Key Laboratory of Orthopedics and Traumatology Department of Spine SurgeryThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510080China Research Center for Translational Medicine The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510080China Precision Medicine Institute The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510080China Department of Orthopedics Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Guangdong Provincial Key Laboratory of Proteomics and State Key Laboratory of Organ Failure Research School of Basic Medical SciencesSouthern Medical UniversityGuangzhouGuangdong 510515China

Mutation of the MAPK7 gene was related to human scoliosis.Mapk7 regulated the development of limb bones and skulls in mice.However,the role of MAPK7 in vertebral development is still unclear.In this study,we constructed Col2a1-cre;Mapk7 f/f transgenic mouse model to delete Mapk7 in cartilage,which displayed kyphosis and osteopenia.Mechanistically,Mapk7 loss decreased MEF2C expression and thus activated PTEN to oppose PI3K/AKT signaling in vertebral growth plate chondrocytes,which impaired chondrocyte hypertrophy and attenuated vertebral ossification.In vivo,systemic pharmacological activation of AKT rescued impaired chondrocyte hypertrophy and alleviated mouse vertebral defects caused by Mapk7 deficiency.Our study firstly clarified the mechanism by which MAPK7 was involved in vertebral development,which might contribute to understanding the pathology of spinal deformity and provide a basis for the treatment of developmental disorders of the spine.

关键词： Chondrocyte hypertrophy Growth plate Kyphosis MAPK7 MEF2C Osteopenia PI3K/AKT PTEN

Effect of manipulation on cartilage in rats with knee osteoarthritis based on the Rho-associated protein kinase/LIM kinase 1/Cofilin signaling pathways

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Journal of Traditional Chinese Medicine 2022年第2期42卷 194-199页

作者： GUO Xiao YANG Yunhao liao dongmei PANG Fang YANG Zhixue ZHU Zhengwei LUO Ao TANG Chenglin College of Traditional Chinese Medicine Chongqing Medical UniversityChongqing 400010China Beibei Traditional Chinese Medicine Hospital Chongqing 400700China

OBJECTIVE:To investigate the effect of manipulation treatment on knee osteoarthritis rats and the effect on Rho-associated protein kinase(ROCK)/LIM-kinase1(LIMK1)/Cofilin signaling pathway.METHOD:Fifty Specific pathogen Free Sprague-Dawley rats were randomly divided into five groups(n=8 each):blank group,model group,manipulation group,celecoxib group,and manipulation combined with celecoxib group(MC group).The osteoarthritis model was established by injecting 0.2 m L 4%papain into the articular disc of the rats.After successfully establishing the model,we treated the manipulation group with pushing manipulation using one-finger-meditation to the Neixiyan(EX-LE4),Waixiyan(EX-LE5),Xuehai(SP10),Liangqiu(ST34),and Zusanli(ST36)acupoints for 10 min each time.Also,the celecoxib group was gavaged with 24 mg·kg^(-1)·d^(-1 )celecoxib,while the MC group was treated using both of these two methods.After four weeks,the cartilage of the right femur was removed for hematoxylin-eosin staining of the cartilage tissue.The expressions of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum were observed using the enzyme-linked immunosorbent assay.Besides,we detected the expressions of ROCK,LIMK1,Phospho-LIM-kinase1(Phospho-LIMK1),Cofilin,and Phospho-Cofilin by Western blot.RESULTS:Compared to the model group,the manipulation group,celecoxib group,and MC group all exhibited superior results concerning pathological morphologic changes of cartilage,as observed by hematoxylin-eosin staining and calculated using the Mankin score.Besides,in contrast to the blank group,the model group exhibited elevated serum levels of IL-1βand TNF-α(P<0.01),while the expression of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage were all higher(P<0.01).Also,the serum levels of IL-1βand TNF-αin each treatment group were lower(P<0.01)than in the model group.Moreover,there were lower expressions of ROCK,LIMK1,Phospho-LIMK1,Cofilin,and Phospho-Cofilin in cartilage in the manipulation group and the MC group(P<0.01).Compared with t

关键词： osteoarthritis knee manipulation cytoskeletal proteins Rho-associated kinases LIM kinases actin depolymerizing factors signal transduction

The effect of manipulation on cartilage in rats with knee osteoarthritis based on the ROCK/LIMK1/Cofilin signaling pathways

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Journal of Traditional Chinese Medicine 2022年

作者： GUO Xiao YANG Yunhao liao dongmei PANG Fang YANG Zhixue ZHU Zhengwei LUO Ao TANG Chenglin College of Traditional Chinese Medicine， Chongqing Medical University Beibei Traditional Chinese Medicine Hospital

OBJECTIVE: To investigate the effect of manipulation treatment on knee osteoarthritis rats and the effect on Rho-associated protein kinase/LIM kinase 1/Cofilin signaling pathway. METHOD: Fifty SPF SD rats were randomly divided into five groups （n = 8 each）: blank group， model group， manipulation group， celecoxib group， and manipulation combined with celecoxib group （MC group）. The osteoarthritis model was established by injecting 0.2 mL 4% papain into the articular disc of the rats. After successfully establishing the model， we treated the manipulation group with pushing manipulation using one-finger-meditation to the Neixiyan （EX-LE4）， Waixiyan （EX-LE5）， Xuehai （SP10）， Liangqiu （ST34）， and Zusanli （ST36） acupoints for 10 minutes each time. Also， the celecoxib group was gavaged with 24 mg·kg;·d;celecoxib， while the MC group was treated using both of these two methods. After four weeks， the cartilage of the right femur was removed for hematoxylin-eosin （HE） staining of the cartilage tissue. The expressions of interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） in serum were observed using the enzyme-linked immunosorbent assay （ELISA）. Besides， we detected the expressions of Rho-associated protein kinase （ROCK）， LIM-kinase1 （LIMK1）， Phospho-LIM-kinase1 （Phospho-LIMK1）， Cofilin， and Phospho-Cofilin by Western blot. RESULTS: Compared to the model group， the manipulation group， celecoxib group， and MC group all exhibited superior results concerning pathological morphologic changes of cartilage， as observed by HE staining and calculated using the Mankin score. Besides， in contrast to the blank group， the model group exhibited elevated serum levels of IL-1β and TNF-α （P < 0.01）， while the expression of ROCK， LIMK1， Phospho-LIMK1， Cofilin， and Phospho-Cofilin in cartilage were all higher （P < 0.01）. Also， the serum levels of IL-1β and TNF-α in each treatment group were lower （P < 0.01） than in the model group. Moreover， there were lower expressions of ROCK， LIMK1， Phospho-LIMK1， Cofilin， and Phospho-Cofilin in c

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火电厂化验室智慧化转型实践

电力安全技术 2024年第5期26卷 1-6页

作者：刘格霞符浩廖冬梅国能长源汉川发电有限公司湖北孝感431614 武汉大学动力与机械学院湖北武汉430072

根据火电厂化验室化学监督业务的特点和管理需求,某公司研制了火电厂化学监督智能管控综合体和LIMS软件。通过设备数据接口的开发实现了检测数据自动采集、智能统计与报送、自动生成关键指标趋势图、检测全流程实时溯源、异常结果自动... 详细信息

根据火电厂化验室化学监督业务的特点和管理需求,某公司研制了火电厂化学监督智能管控综合体和LIMS软件。通过设备数据接口的开发实现了检测数据自动采集、智能统计与报送、自动生成关键指标趋势图、检测全流程实时溯源、异常结果自动告警等功能;同时基于智能手持终端,实现了操作人员户外取样与就地检验、数据实时上传、自动生成监督报表和报告以及危化品库的智能安全管理。该智慧化转型实践,促进了流程的科学化、规范化和标准化,提高了数据的共享、融通能力以及工作效率。

关键词：火电厂化验室智慧化化学监督智能管控综合体

耳穴贴压联合低频电刺激治疗产后尿潴留的应用效果

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中国医药指南 2024年第14期22卷 142-144页

作者：谢菊红朱秀梅廖冬梅赣州市于都县妇幼保健院赣州342300

目的通过进行严格的科研设计,探讨耳穴贴压对产后尿潴留的治疗效果。方法选取我院2023年6月—2023年12月期间收治的分娩并发产后尿潴留的产妇92例作为研究对象,按随机数字表法分为传统干预组和耳穴+电刺激组,各46例。传统干预组采用常... 详细信息

目的通过进行严格的科研设计,探讨耳穴贴压对产后尿潴留的治疗效果。方法选取我院2023年6月—2023年12月期间收治的分娩并发产后尿潴留的产妇92例作为研究对象,按随机数字表法分为传统干预组和耳穴+电刺激组,各46例。传统干预组采用常规听流水声,温水清洗,新斯的明肌注,加上低频电刺激治疗。耳穴+电刺激组在传统干预组治疗的基础上,增加耳穴贴压。比较两组产妇干预后首次自主排尿时间、排尽尿液后膀胱残余尿量以及治疗总有效率。结果耳穴+电刺激组干预后首次自主排尿时间短于传统干预组,排尽尿液后膀胱残余尿量低于传统干预组(均P<0.05)。耳穴+电刺激组的治疗总有效率100%比传统干预组的78.26%高(P<0.05)。结论耳穴贴压联合低频电刺激治疗产后尿潴留的应用效果确切,可提高治疗总有效率,加速患者自主排尿的恢复。

关键词：耳穴贴压低频电刺激产后尿潴留

反刍思维在精神分裂症患者童年创伤经历与认知功能之间的中介作用

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国际精神病学杂志 2024年第1期51卷 59-62页

作者：廖翠青王冬梅詹晓宇中国科学院心理研究所中国科学院心理健康重点实验室北京100101 中国科学院大学心理学系泉州神和精神专科医院

目的探讨精神分裂症患者童年创伤经历与认知功能损害及反刍思维的中介作用。方法选取2020年1月~2022年10月,精神专科医院收治的132例精神分裂症患者。采用童年创伤问卷(Childhood Trauma Questionnaire,CTQ),反刍反应量表(Ruminative Re... 详细信息

目的探讨精神分裂症患者童年创伤经历与认知功能损害及反刍思维的中介作用。方法选取2020年1月~2022年10月,精神专科医院收治的132例精神分裂症患者。采用童年创伤问卷(Childhood Trauma Questionnaire,CTQ),反刍反应量表(Ruminative Response Scale,RRS),阳性与阴性症状量表(Positive and Negative Symptoms Scale,PANSS),可重复的成套神经心理状态测量(Repeatable Battery for the Assessment of Neuro psychological Status,RBANS)量表评估患者的童年创伤,反刍思维,阳性阴性症状和认知功能。结果有童年创伤经历组精神分裂症患者的反刍症状,强迫思考,反省深思高于无童年创伤经历组(均P<0.001);有童年创伤经历组PANSS总分和因子分高于无童年创伤经历组(均P<0.01)。有童年创伤经历组的RBANS总分及因子分即刻记忆,空间结构,语言,注意力,延迟记忆得分高于无童年创伤经历组(均P<0.01)。线性回归分析显示反刍思维在童年创伤经历与认知功能之间起中介作用(P<0.001)。结论有童年创伤经历的精神分裂症患者的认知功能低于无童年创伤经历组,反刍思维在童年创伤经历与认知功能之间起中介作用。

关键词：童年创伤经历反刍思维认知功能精神分裂症

星状神经节离断对脊髓损伤大鼠中枢性疼痛行为及CREB SGK1 NF-κB蛋白表达的影响

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中国实用神经疾病杂志 2024年第3期27卷 271-276页

作者：邓莉丽毛冬梅廖泽青姚旌贵州医科大学研究生院贵州贵阳550004 贵州医科大学附属医院贵州贵阳550004

目的探究星状神经节离断对脊髓损伤(SCI)大鼠中枢性疼痛行为及环磷腺苷效应元件结合蛋白(CREB)、血清和糖皮质激素调节蛋白激酶1(SGK1)、核因子-κB(NF-κB)蛋白表达的影响。方法将SD大鼠按随机分配原则分为假手术组(Sham组)、模型组(Mo... 详细信息

目的探究星状神经节离断对脊髓损伤(SCI)大鼠中枢性疼痛行为及环磷腺苷效应元件结合蛋白(CREB)、血清和糖皮质激素调节蛋白激酶1(SGK1)、核因子-κB(NF-κB)蛋白表达的影响。方法将SD大鼠按随机分配原则分为假手术组(Sham组)、模型组(Model组)、治疗组(Treat组)各6例。Model组、Treat组构建SCI模型,Sham组未使用铁球砸脊髓,其他步骤相同。Treat组在脊髓损伤后予星状神经节离断,通过BBB运动功能评分评价运动功能学,通过机械痛阈试验和热痛阈试验测量机械缩足反射阈值(PWT)和热缩足潜伏期(PWL),ELISA法检测血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)水平,HE染色检测脊髓组织病理变化,TUNEL染色检测细胞凋亡,Western blot检测CREB、SGK1、NF-κB p65蛋白表达。结果Sham组大鼠脊髓组织结构完好,细胞呈正常形态,神经纤维分布均匀且行走规则。与Sham组相比,Model组脊髓组织及细胞完整性破坏严重,形成大量空洞,神经纤维排列紊乱,可见大量炎性细胞浸润,BBB运动功能评分、PWT、PWL值显著下降(P<0.05),IL-1β、TNF-α、PGE2水平、细胞凋亡数量、CREB、SGK1、NF-κB p65蛋白水平显著升高(P<0.05)。与Model组相比,Treat组脊髓组织结构形态明显改善,脊髓组织空洞化有所好转,炎性细胞浸润减少,神经纤维排列趋于正常,BBB运动功能评分、PWT、PWL值显著增加(P<0.05),IL-1β、TNF-α、PGE2水平、细胞凋亡数量、CREB、SGK1、NF-κB p65蛋白水平显著减少(P<0.05)。结论星状神经节离断可减轻SCI大鼠中枢性疼痛行为,降低CREB、SGK1、NF-κB蛋白水平。

关键词：星状神经节离断脊髓损伤中枢性疼痛行为环磷腺苷效应元件结合蛋白血清和糖皮质激素调节蛋白激酶1 核因子-κB 大鼠

金银花与山银花活性成分抗炎作用及机制研究进展

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中国实验方剂学杂志 2024年第11期30卷 273-281页

作者：韦晶玥罗诗雯冯龄燃林万军吴可青廖旭辉庹勤慧杨冬梅湖南中医药大学血管生物学与转化医学湖南省重点实验室长沙410208 湖南中医药大学中西医结合防治血管疾病基础研究中心长沙410208

炎症参与了机体各种急慢性疾病的发展,持续的炎症反应是癌症、神经退行性疾病、心血管疾病、代谢综合症、炎症性肠病、关节炎等多种疾病的关键驱动因素,因此寻找抗炎药物对于防治各类疾病至关重要。近年来人们愈发重视寻找毒不良反应小... 详细信息

炎症参与了机体各种急慢性疾病的发展,持续的炎症反应是癌症、神经退行性疾病、心血管疾病、代谢综合症、炎症性肠病、关节炎等多种疾病的关键驱动因素,因此寻找抗炎药物对于防治各类疾病至关重要。近年来人们愈发重视寻找毒不良反应小的天然药物,金银花和山银花作为传统清热解毒类中药具有较强的生物活性和多种药理功效,在植物界中广泛分布,药用价值显著。随着金银花和山银花研究的不断推进,其在医药领域被广泛应用,极具开发潜力。目前,有关研究多集中在金银花和山银花的抗炎作用机制上,而对其抗炎活性成分的系统总结鲜有报道。因此,该文重点对金银花和山银花二者抗炎活性成分进行了差异分析,并综述了金银花和山银花提取物及活性成分可能通过影响细胞炎症因子释放、调节转录因子-κB(NF-κB)、丝裂原活化蛋白激酶(MAPK)、信号转导和转录激活因子3(STAT3)、MAPK/胞外信号调节激酶(ERK)/c-Jun氨基末端激酶(JNK)、磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)/NF-κB、Janus激酶(JAK)/信号转导与转录激活因子(STAT)等炎症信号通路、增加抗氧化应激能力、增强免疫防御能力、改善肠道微生态等多途径发挥抗炎作用的机制,以期为二者临床合理应用提供理论基础。

关键词：金银花山银花活性成分抗炎作用机制

OsCPL3 is involved in brassinosteroid signaling by regulating OsGSK2 stability

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Journal of Integrative Plant Biology 2022年第8期64卷 1560-1574页

作者： Luping Gong Shenghao liao Wen Duan Yongqiang Liu dongmei Zhu Xiaosheng Zhou Baoping Xue Chengcai Chu Yun-Kuan Liang State Key Laboratory of Hybrid Rice Department of Plant SciencesCollege of Life SciencesWuhan UniversityWuhan 430072China Hubei Hongshan Laboratory Wuhan 430070China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China

Glycogen synthase kinase 3(GSK3)proteins play key roles in brassinosteroid(BR)signaling during plant growth and development by phosphorylating various substrates.However,how GSK3 protein stability and activity are themselves modulated is not well understood.Here,we demonstrate in vitro and in vivo that C-TERMINAL DOMAIN PHOSPHATASELIKE 3(Os CPL3),a member of the RNA Pol II CTD phosphatase-like family,physically interacts with Os GSK2 in rice(Oryza sativa).Os CPL3 expression was widely detected in various tissues and organs including roots,leaves and lamina joints,and was induced by exogenous BR treatment.Os CPL3 localized to the nucleus,where it dephosphorylated Os GSK2 at the Ser-222 and Thr-284 residues to modulate its protein turnover and kinase activity,in turn affecting the degradation of BRASSINAZOLERESISTANT 1(BZR1)and BR signaling.Loss of Os CPL3 function resulted in higher Os GSK2 abundance and lower Os BZR1 levels,leading to decreased BR responsiveness and alterations in plant morphology including semi-dwarfism,leaf erectness and grain size,which are of fundamental importance to crop productivity.These results reveal a previously unrecognized role for Os CPL3 and add another layer of complexity to the tightly controlled BR signaling pathway in plants.

关键词： brassinosteroids OsCPL3 OsGSK2 plant architecture rice productivity