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Generating Microstructures with Highly Variable Mechanical Performance using Two-Photon Lithography and Thiol-ene Photopolymerization

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Chinese Journal of Polymer Science 2023年第1期41卷 67-74页

作者： Xiao Yang Yan-Fang Niu Meng-Xiao Wei Jun-Ning Zhang ke-liang Liu Xin Du Zhong-Ze Gu State key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China

In this study,we investigate the effect of the exposure dose on the mechanical property of the photoresins generated with acrylate self-polymerization and thiol-ene *** results indicate that the mechanical performance of the thiol-ene photoresin is highly depended on the exposure dose during the solidification *** 350-fold exposure dose change,the stiffness of the thiol-ene photoresin reached more that 700-fold change compare to 14-fold of the acrylate *** developed a TPL photoresist based on our results and show the capability to fabricate microstrucutres with high resolution and variable mechanical performances using this method.

关键词： Thiol-ene Two photo lithography 3D printing Photoresist

Unraveling magnetic properties and martensitic transformation in Mn-rich Ni-Mn-Sn alloys:first-principles calculations and experiments

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Rare Metals 2024年第4期43卷 1769-1785页

作者： Yu Zhang Jing Bai ke-liang Guo Jia-Xin Xu Jiang-Long Gu Nicola Morley Qui-Zhi Gao Yu-Dong Zhang Claude Esling Xiang Zhao liang Zuo key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Material Science and EngineeringNortheastern UniversityShenyang110819China key Laboratory of Dielectric and Electrolyte Functional Material Hebei Province School of Resources and MaterialsNortheastern University at QinhuangdaoQinhuangdao066004China Department of Material Science and Engineering University of SheffieldSheffieldS13JDUK State key Laboratory of Metastable Materials Science and Technology Yanshan UniversityQinhuangdao066004China Laboratoire d’Étude des Microstructures Et de Mécanique des Matériaux University of Lorraine57045MetzFrance

We have investigated the phase stability,magnetic properties,and martensitic transformation thermodynamics/kinetics of the Ni_(24-x)Mn_(18+x+y)Sn_(6-y)(x,y=0,1,2)system by combining the first-principles calculations and *** calculation results show that the optimized lattice parameters are consistent with the experimental ***,we obtain the relation equation for the austenite formation energy(E_(form-A))and Mn content(X_(Mn)):E_(form-A)=507.358X_(Mn)-274.126,as well as for the six-layer modulated(6M)martensite formation energy(E_(form-6M))and Ni content(X_(Ni)):E_(form-6M)=-728.484X_(Ni)+*** ternary phase diagram of the total magnetic moment was *** excess Mn will reduce the total magnetic moment of 6M(Mag6M)and non-modulated(NM)(MagNM)martensites,with the following equations relating the total magnetic moment and Mn content:Mag_(6M)=-15.905X_(Mn)+7.902and Mag_(NM)=-14.781X_(Mn)+7.411,while the effect on austenite is *** variation of total magnetic moment is mainly dominated by the Mn atomic magnetic *** 3d electrons of Mn_(Sn)(Mn at Sn sublattice)play an important role in magnetic properties from the perspective of the electronic density of *** on the thermodynamics of martensitic transformation,the alloys will likely undergo austenite?6M?NM transformation *** the thermodynamic and kinetic results,the martensitic transformation temperature decreases with x increasing and increases with y *** results are expected to provide reference for predicting the phase stability and magnetic properties of NiMn-Sn alloys.

关键词： Ni-Mn-Sn First-principles calculations Martensitic transformation Magnetic property Kinetics

Parallel DNA Constrained by“CC^+Clamps”

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Chemical Research in Chinese Universities 1999年第2期15卷 42-47页

作者： LIU ke-liang H.Todd Miles Frank B.Howard Joe Frazier V.Sasisekharan Laboratory of Molecular Biology NIDDKNational Institutes of HealthBethesdaMaryland20892USA

Parallel DNA helices with reverse WatsonCrick pairing have been described in several *** has been pointed out,however,that the parallel structures may also be in equilibrium with antiparallel WatsonCrick helices due to their closely similar *** avoid the problem we have prepared and characterized DNA helices which are constrained to possess parallel polarity by two dC residues,or CC+clamps,at both the ends of AT oligomer *** residues are hemiprotonated below neutral pH,forming two stable CC+base pairs with three hydrogen bonds at each end of the *** physical properties of these constructs can then be used to calibrate those of duplexes having the same sequences but lacking the terminal C *** recent studies indicate that dC acid selfstructures are fourstranded with intercalated bases,gel electrophoresis indicates that these structures are not present in the molecules described here.

关键词： Parallel DNA helices Reverse Watson-Crick pairing CC+ base pair Oligonucleotide helices, Reverse WatsonCrick pairing, CC+ base pair, Oligonucleotide

Differentially methylated genomic fragments related with sexual dimorphism of rice pests, Sogatella furcifera

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Insect Science 2015年第6期22卷 731-738页

作者： Mei Zhang Jia-Lin Chen Shi-ke liang Guang-Hong Li Fang-Hai Wang Ijaz Ahmad State key Laboratory for Biocontrol and Institute of Entomology Sun Yat-sen University Guangzhou 510275 China Departmentof Chemistry Kohat University of Science & Technology Kohat Pakistan

Sogatella furcifera （Hovarth） is a major rice pest with sexual dimorphism. The objective of the current research was to monitor differentially cytosine methylation at CCGG sequences in male and female adults of S. furcifera to determine the association between gene methylation and sexual phenotypes using methylation-sensitive representa- tional difference analysis. After the second subtractive hybridization, four differentially methylated DNA bands were obtained and sequenced. Ten different fragments were found. One fragment from the positive hybridization was 120 bp, and highly similar to the tram- track genes from Nasonia vitripennis. Another fragment from the reverse hybridization was 414 bp, and homologous to the 28S rRNA gene of S. furcifera with a similarity rate as high as 99%. We also discussed how DNA methylation of tramtrack and 28S rRNA genes produced effects on sexual differentiation and development. These results provide potential evidence that DNA methylation of some genes may be related to sexual phenotype variations in *** and will facilitate future studies on the epigenetic mechanisms of insect sexual dimorphism.

关键词： cytosine methylations epigenetics methylation-sensitive representationaldifference analysis rice planthopper sexual dimorphism tramtrack

EHMT2 (G9a) activation in mantle cell lymphoma and its associated DNA methylation and gene expression

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Cancer Biology & Medicine 2022年第6期19卷 836-849页

作者： Jun Wang Hui Xu Shuang Ge Chaoshuai Xue Hailing Li Xiaotong Jing ke liang Xiaoying Zhang Cuijuan Zhang International License institute of Pathology and Pathophysiology Shandong University School of MedicineJinan 250012China Department of Pathology Qianfoshan HospitalJinan 250300China Department of Pathology Qilu Hospital of Shandong UniversityJinan 250012China

Objective:The function of euchromatic histone-lysine N-methyltransferase 2(EHMT2)has been studied in several cancers;however,little is known about its role in mantle cell lymphoma(MCL).Thus,this study aimed to characterize the significance and function of EHMT2 in ***:EHMT2 expression in MCL and reactive hyperplasia(RH)were investigated by ***-wide analysis of DNA methylation was performed on EHMT2+MCL *** function of EHMT2 was determined by CCK&flow cytometry,and western blot *** expression profile analysis was performed before and after EHMT2 knockdown to search for EHMT2-regulated ***-immunoprecipitation(Co-IP)experiments were conducted to identify the proteins interacting with ***:EHMT2 was expressed in 68.57%(24/35)of MCLs but not in any ***-wide analysis of DNA methylation on EHMT2+MCLs revealed that multiple members of the HOX,FOX,PAX,SOX,and CDX families were hypermethylated or hypomethylated in EHMT2+***0I294,a EHMT2 inhibitor,inhibited MCL cell growth and stalled cells in the G1 ***,BIX01294 downregulated the expressions of cell cycle proteins,cyclin DI,CDK4,and P21,but upregulated the expressions of apoptosis-related proteins,Bax and ***-IP experiments revealed that EHMT2 interacted with UHRF1,HDAC1,and HDAC2 but not with *** EHMT2 knockdown,multiple genes were regulated,including CD5 and CCND1,mostly enriched in the Tec kinase signaling *** addition,several genes(e.g.,MARCH 1,CCDC50,HIP1,and WNT3)were aberrantly methylated in EHMT2+***:For the first time,we determined the significance of EHMT2 in MCL and identified potential EHMT2-regulated genes.

关键词： DNA methylation mantle cell lymphoma epigenetic biomarker EHMT2 gene expression

Microstructural Evolution of Gas Atomized Fe-25Cr-3.2C Alloy Powders

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Journal of Iron and Steel Research International 2011年第2期18卷 75-78页

作者： YANG Min SONG Chang-jiang DAI Yong-xiang ZHU liang LI ke-feng ZHAI Qi-jie Shanghai key Laboratory of Modern Metallurgy and Materials Processing Shanghai University Shanghai 200072 China

The microstructural evolution of the gas atomized Fe-25Cr-3.2C powders was investigated by using optical microscope, scanning electron microscope, and X-ray diffraction. The experimental results showed that the atomized Fe-25Cr-3.2C powders were mainly composed of austenite and （Fe,Cr）7 C3 carbide. Eutectic microstructure was developed in the larger particles, whereas dendritic microstrueture was obtained in the particles with diameter less than 38 μm. The reason for microstructure change should be the difference of nucleation undercooling for particles.

关键词： gas atomization Fe-Cr-C alloy microstructure undercooling rapid solidification

Ni/ZSM-5 Catalysts for Light Olefin Oligomerization:Effects of Supports and Ni Sites on Activity and Selectivity

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China Petroleum Processing & Petrochemical Technology 2024年第3期26卷 15-23页

作者： Zuo Qi liang ke Ma Yirong Jia Yangxiao Liao Mingjie Zheng Jiajun Li Ruifeng Li Wenlin College of Chemistry and Chemical Engineering Taiyuan University of TechnologyTaiyuanShanxi 030024China

A series of Ni/ZSM-5 containing a small amount of Ni was prepared by an ion exchanged *** impact of the n(SiO_(2))/n(Al_(2)O_(3))ratio on the catalytic activity was studied using the samples 0.09Ni/ZSM-5(60)and 0.09Ni/ZSM-5(130).To determine the interaction between the Ni species and acid sites on the surface of the catalyst,the catalysts were characterized by N2 adsorption-desorption,X-ray diffraction(XRD),scanning electron microscopy(SEM),and UV-vis *** performance of the catalysts for the catalytic oligomerization of 1-hexene was investigated in *** nickel species were found to be uniformly distributed in all the *** was discovered that the oligomerization activity of the catalyst can be improved using Ni species;however,the contribution of Brønsted acids in oligomerization reactions is greater than that of Ni sites and Lewis acids.

关键词： nickel zeolite acid sites oligomerization catalyst

Strategies for the Construction of Vernacular Landscape in Urban Gardens

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Journal of Landscape Research 2020年第4期12卷 107-110页

作者： liang ke School of Architecture and Design China University of Mining and TechnologyXuzhouJiangsu 221116China

Vernacular landscape is the carrier of traditional techniques and local *** construction of vernacular landscape has gradually become the basic direction of modern urban garden *** on the analysis of the extraction and application of vernacular landscape elements,the application forms and design principles of each element in urban gardens are pointed *** the same time,taking the artistic expression method of integrating vernacular landscape elements into garden landscape as the starting point and combining with case analysis,the design principles and main planning points of building vernacular landscape are put forward,and some strategies for the construction and protection of vernacular landscape in the planning of garden landscape in different regions are proposed.

关键词： Urban gardens Vernacular landscape elements Expression method Construction strategies

Modified unified co-rotational framework with beam,shell and brick elements for geometrically nonlinear analysis

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Acta Mechanica Sinica 2022年第4期38卷 116-127,I0003页

作者： Yufei Rong Qin Sun ke liang School of Aeronautics Northwestern Polytechnical UniversityXi'an 710072China

The co-rotational finite element formulation is an attractive technique extending the capabilities of an existing high performing linear element to geometrically nonlinear *** paper presents a modified co-rotational framework,unified for beam,shell,and brick elements.A unified zero-spin criterion is proposed to specify the local element frame,whose origin is always located at the *** this criterion,a spin matrix is introduced,and the local frame is invariant to the element nodal ***,the projector matrix is redefined in a more intuitive way,which is the derivative of local co-rotational element frame with respect to the global ***,the nodal rotation is obtained with pseudo vector and instantaneous rotation,under a high-order accurate *** resulting formulations are achieved in unified expression and thus a series of linear elements can be embedded into the *** examples are presented to demonstrate the efficiency and accuracy of the proposed framework for large displacement analysis.

关键词： Geometrically nonlinear analysis Modified co-rotational framework Unified zero-spin criterion Spin matrix Projector