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Increased expression of insulin-like growth factor-binding protein-3 is implicated in erectile dysfunction in two-kidney one-clip hypertensive rats after propranolol treatment

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Asian Journal of Andrology 2011年第6期13卷 851-855页

作者： Zhang-Yan Zhou Zhong-hua Yang Xing-huan wang Hong Cao Dong chen Yong-Zhi wang Hai-Hong Zhou Mou Peng Quan-liang Liu Shao-Ping wan Department of Urology Zhongnan Hospital of Wuhan University Wuhan 430071 China

This study aimed to investigate the role of insulin-like growth factor-binding protein-3 （IGFBP-3） in erectUe dysfunction （ED） in two-kidney one-clip （2K-1C） hypertensive rats treated with the β-blocking agent propranolol. Adult male Wistar rats were randomly divided into three groups： a normal control group, a hypertensive control group and a propranolol treatment group （n=9）. After 4 weeks of propranolol treatment, intracavemous pressure （ICP） responses to electrical stimulation of the cavernous nerves were evaluated. The expression of IGFBP-3 and insulin-like growth factor-1 （IGF-1） mRNA and protein in the rat cavernous tissue were detected by quantitative real-time PCR and Western blot, respectively. The concentration of cyclic guanosine monophosphate （cGMP） in the cavernous tissue was determined by enzyme-linked immunosorbent assay （ELISA）. Cavernosal pressure in response to cavernous nerve stimulation was decreased 4 weeks after propranolol treatment （P〈0.01, compared to the hypertensive control group）. IGFBP-3 mRNA and protein expression was increased in the propranolol treatment group compared to the hypertensive control group （P〈O.01）, whereas IGF-1 expression was decreased in the propranolol treatment group compared to the hypertensive control group （P〈0.01）. In addition, cavernous cGMP concentration was decreased in the prepranolol treatment group compared to the hypertensive control group （P〈0.01）. Taken together, these results suggest that the upregulation of IGFBP-3 may play a role in the development of ED in hypertensive rats.

关键词： erectile dysfunction insulin-like growth factor-binding protein-3 propranolol two-kidney one-clip hypertension

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Microsurgical subinguinal varicocelectomy with spermatic cord double traction and vein stripping

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Asian Journal of Andrology 2020年第2期22卷 208-212页

作者： Ru-Hui Tian liang-Yu Zhao Hui-Xing chen Chao Yang Peng Li Yu-hua huang Zhong wan Er-Lei Zhi chen-cheng Yao Zheng Li Department of Andrology Center for Men’s HealthDepartment of ARTUrologic Medical CenterShanghai General HospitalShanghai Key Lab of Reproductive MedicineShanghai Jiao Tong UniversityShanghai 200080China

We retrospectively reviewed data for 286 patients with varicocele who underwent microsurgical subinguinal varicocelectomy from March 2015 to May 2017 in Shanghai General Hospital(Shanghai,China).In this surgical approach,the testis was delivered,and the gubernacular and external cremasteric veins were *** addition,the spermatic cord was delivered downward with continuous double traction away from the external *** remaining procedure was similar to the conventional *** followed patients for at least 3 months and evaluated postoperative semen parameters,pain symptoms,and *** excluded data for 32 men due to inadequate follow-up(<3 months).Of the remaining 254 patients,73 had oligoasthenospermia,121 had nonobstructive azoospermia,and 60 had symptomatic *** progressive sperm counts increased in the oligoasthenospermic patients from a median preoperative value of 9.15×10^6 ml^?1 to 25.33×10^6 ml^?1(n=34),and 35.6%(26/73)initially oligoasthenospermic men contributed to unassisted *** returned to the ejaculate in 12.4%(15/121)azoospermia *** patients with scrotal pain(n=60),43(71.7%)reported complete resolution of pain,16(26.7%)reported partial resolution,and 1(1.7%)reported no *** patients experienced varicocele *** doubletraction strategy avoids opening the external oblique aponeurosis,and results in less damage and faster *** addition,the stripping strategy eliminates potential damage to the testis caused by the varicose *** results showed that microsurgical subinguinal varicocelectomy using spermatic cord double traction in conjunction with testicular delivery for vein stripping is a safe and effective approach for varicocele repair.

关键词： azoospermia low sperm count sperm motility spermatic cord varicocele

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基于玻璃毛细管的大气环境MeV质子微束的产生与测量

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物理学报 2024年第10期73卷 143-152页

作者：万城亮潘俞舟朱丽萍李鹏飞张浩文赵卓彦袁华樊栩宏孙文胜杜战辉陈乾崔莹廖天发魏晓慧王天琦陈熙萌李公平 Reinhold Schuch 张红强兰州大学核科学与技术学院兰州730000 兰州大学基础医学院兰州730000 哈尔滨工程大学核科学与技术学院哈尔滨150001 南通大学理学院南通226019 惠州学院电子信息与电气工程学院惠州516067 哈尔滨工业大学空间环境与物质科学研究院哈尔滨150006 兰州大学稀有同位素前沿科学中心兰州730000 斯德哥尔摩大学物理系斯德哥尔摩S-10691

本文采用玻璃毛细管产生了大气环境中工作的2.5 MeV质子外束微束,并对束斑直径及能量分布随玻璃毛细管与束流方向之间角度(倾角)变化进行测量.测量结果表明,在玻璃毛细管轴向与束流方向一致时(倾角为0°),产生的微束中存在保持初始入射... 详细信息

本文采用玻璃毛细管产生了大气环境中工作的2.5 MeV质子外束微束,并对束斑直径及能量分布随玻璃毛细管与束流方向之间角度(倾角)变化进行测量.测量结果表明,在玻璃毛细管轴向与束流方向一致时(倾角为0°),产生的微束中存在保持初始入射能量的直接穿透部分以及散射部分,其中直接穿透的质子占比最大,束斑直径也最大.随着玻璃毛细管倾角的增大,当其大于几何张角时,束斑直径变小,产生的微束全部为能量减小的散射部分,直接穿透质子消失.我们对质子在玻璃毛细管内传输时的内壁散射过程进行了模拟计算及离子轨迹分析,发现大角度的散射部分决定了形成的外束微束斑外围轮廓,而束斑中心区域由不与毛细管内壁产生任何作用的直接穿透离子构成,其大小由玻璃毛细管出口直径以及几何容许张角决定.采用玻璃毛细管产生的外束微束具有产生简单廉价,微束区域定位简单的特点,有望在辐射生物学、医学、材料等领域得到广泛应用.

关键词：离子外束微束 MeV质子锥形玻璃毛细管

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流动人工体液中冠状动脉支架用奥氏体不锈钢的电化学机理研究

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生物医学工程学杂志 2005年第4期22卷 730-733页

作者：梁成浩郭亮陈婉王华大连理工大学化工学院大连116012

采用电化学测试技术在动态循环人工模拟体液中对冠状动脉支架用SUS316L和SUS317不锈钢的电化学机理进行研究。结果表明,流动环境降低了不锈钢的点蚀电位Eb值,提高了点蚀敏感性,促进了阳极活性溶解,但对再钝化电位Ep值影响不大。流动对... 详细信息

采用电化学测试技术在动态循环人工模拟体液中对冠状动脉支架用SUS316L和SUS317不锈钢的电化学机理进行研究。结果表明,流动环境降低了不锈钢的点蚀电位Eb值,提高了点蚀敏感性,促进了阳极活性溶解,但对再钝化电位Ep值影响不大。流动对奥氏体不锈钢试样的阴极极化行为有着显著影响,介质流动使氧到达电极表面的传质控制层减薄,加速氧的传质,增大阴极极化电流密度,并随流速的提高阴极吸氧反应加快。

关键词：流动冠状动脉支架不锈钢电化学机理奥氏体不锈钢机理研究人工体液人工模拟体液电化学测试氧反应

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Expression of NF-κ B and osteopontin of synovial fluid of patients with knee osteoarthritis

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Asian Pacific Journal of Tropical Medicine 2013年第5期6卷 379-382页

作者： Li-Feng Qin wan-Chun wang Han Fang Xin-Zhan Mao Guo-liang huang You chen Hou-De Zhou Yi Shen Li-hua Qin Dan Peng Department of Orthopedics Second Xiang-Ya HospitalCentral South University Department of Cardiovascular Disease Xiang-Ya Hospital Central South University Department of Endocrinology Second Xiang-Ya HospitalCentral South University Department of Gynecology Nursing School of Hunan University of Chinese Medicine

Objective:To explore the significance of osteopontin and nuclear factorκB(NF-κB) expression in patients with knee ***:RT-PCR and enzyme-linked immunosorbent assay were used to measure the Osteopontin(OPN) and NF-κB concentration of knee joint synovial fluid of patients with knee osteoarthritis and trauma fractures,and analyze the relationship between the expressiones of ***:OPN and NF-κB expression at the mRNA and protein levels of patients with knee osteoarthritis were significantly higher than the control group, the result showed statistical significance(P<0.05).There was a positive correlation between the OPN levels in synovial fluid of patients with knee osteoarthritis and NF-κB expression levels (P<0.05).Conclusions:The high expression of OPN and NF-κB are closely related to occurrence and development of knee osteoarthritis.

关键词： Osteoarthritis Osteopontin Synovial fluid

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STCF conceptual design report (Volume 1): Physics & detector

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Frontiers of physics 2024年第1期19卷 1-154页

作者： M.Achasov X.C.Ai L.P.An R.Aliberti Q.An X.Z.Bai Y.Bai O.Bakina A.Barnyakov V.Blinov V.Bobrovnikov D.Bodrov A.Bogomyagkov A.Bondar I.Boyko Z.H.Bu F.M.Cai H.Cai J.J.Cao Q.H.Cao X.Cao Z.Cao Q.Chang K.T.Chao D.Y.chen H.chen H.X.chen J.F.chen K.chen L.L.chen P.chen S.L.chen S.M.chen S.chen S.P.chen W.chen X.chen X.F.chen X.R.chen Y.chen Y.Q.chen H.Y.cheng J.cheng S.cheng T.G.cheng J.P.Dai L.Y.Dai X.C.Dai D.Dedovich A.Denig I.Denisenko J.M.Dias D.Z.Ding L.Y.Dong W.H.Dong V.Druzhinin D.S.Du Y.J.Du Z.G.Du L.M.Duan D.Epifanov Y.L.Fan S.S.Fang Z.J.Fang G.Fedotovich C.Q.Feng X.Feng Y.T.Feng J.L.Fu J.Gao Y.N.Gao P.S.Ge C.Q.Geng L.S.Geng A.Gilman L.Gong T.Gong B.Gou W.Gradl J.L.Gu A.Guevara L.C.Gui A.Q.Guo F.K.Guo J.C.Guo J.Guo Y.P.Guo Z.H.Guo A.Guskov K.L.Han L.Han M.Han X.Q.Hao J.B.He S.Q.He X.G.He Y.L.He Z.B.He Z.X.Heng B.L.Hou T.J.Hou Y.R.Hou C.Y.Hu H.M.Hu K.Hu R.J.Hu W.H.Hu X.H.Hu Y.C.Hu J.hua G.S.huang J.S.huang M.huang Q.Y.huang W.Q.huang X.T.huang X.J.huang Y.B.huang Y.S.huang N.Hüsken V.Ivanov Q.P.Ji J.J.Jia S.Jia Z.K.Jia H.B.Jiang J.Jiang S.Z.Jiang J.B.Jiao Z.Jiao H.J.Jing X.L.Kang X.S.Kang B.C.Ke M.Kenzie A.Khoukaz I.Koop E.Kravchenko A.Kuzmin Y.Lei E.Levichev C.H.Li C.Li D.Y.Li F.Li G.Li G.Li H.B.Li H.Li H.N.Li H.J.Li H.L.Li J.M.Li J.Li L.Li L.Li L.Y.Li N.Li P.R.Li R.H.Li S.Li T.Li W.J.Li X.Li X.H.Li X.Q.Li X.H.Li Y.Li Y.Y.Li Z.J.Li H.liang J.H.liang Y.T.liang G.R.Liao L.Z.Liao Y.Liao C.X.Lin D.X.Lin X.S.Lin B.J.Liu C.W.Liu D.Liu F.Liu G.M.Liu H.B.Liu J.Liu J.J.Liu J.B.Liu K.Liu K.Y.Liu K.Liu L.Liu Q.Liu S.B.Liu T.Liu X.Liu Y.W.Liu Y.Liu Y.L.Liu Z.Q.Liu Z.Y.Liu Z.W.Liu I.Logashenko Y.Long C.G.Lu J.X.Lu N.Lu Q.F.Lü Y.Lu Y.Lu Z.Lu P.Lukin F.J.Luo T.Luo X.F.Luo Y.H.Luo H.J.Lyu X.R.Lyu J.P.Ma P.Ma Y.Ma Y.M.Ma F.Maas S.Malde D.Matvienko Z.X.Meng R.Mitchell A.Nefediev Y.Nefedov S.L.Olsen Q.Ouyang P.Pakhlov G.Pakhlova X.Pan Y.Pan E.Passemar Y.P.Pei H.P.Peng L.Peng X.Y.Peng X.J.Peng K.Peters S.Pivovarov E.Pyata B.B.Qi Y.Q.Qi W.B.Qian Y.Qian C.F.Qiao J.J.Qin J.J.Qin L.Q.Qin X.S.Qin T.L.Qiu J.Rademacker C.F.Redmer H.Y.Sang Anhui University Hefei 230039China Beihang University Beijing 100191China Budker Institute of Nuclear Physics Novosibirsk 630090Russia CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China Cavendish Laboratory University of CambridgeJJ Thomson AveCambridge CB30HEUnited Kingdom Central China Normal University Wuhan 430079China Central South University Changsha 410083China China University of Geosciences Wuhan 430074China China University of Mining and Technology Xuzhou 221116China École Polytechnique Fédérale de Lausanne(EPFL) LausanneSwitzerland Fudan University Shanghai 200433China Goethe University Frankfurt D-60325 Frankfurt am MainGermany Guangxi Normal University Guilin 541004China Guangxi Uninversity Nanning 530004China Hangzhou Institute for Advanced Study UCASHangzhou 310024China Hebei Normal University Shijiazhuang 050024China Hebei University Baoding 071002China Hefei University of Technology Hefei 230601China Helmholtz Institute Mainz Staudinger Weg 18D-55099 MainzGermany Henan Normal University Xinxiang 453007China Henan University Kaifeng 475004China High Energy Physics Center Chung-Ang UniversitySeoul 06974Korea Higher School of Economy 11 Pokrovsky Bulvar Moscow 109028Russia huangshan University Huangshan 245000China Hubei University of Automotive Technology Shiyan 442002China Hunan Normal University Changsha 410081China Hunan University of Science and Technology Xiangtan 411201China Hunan University Changsha 410082China Indiana University BloomingtonIndiana 47405USA Inner Mongolia University Hohhot 010021China Institute of Advanced Science Facilities Shenzhen 518107China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China Institute of Physics Academia SinicaTaipeiTaiwan 11529China Institute of Theoretical Physics Chinese Academy of SciencesBeijing 100190China Jilin University Changch

The superτ-charm facility(STCF)is an electron–positron collider proposed by the Chinese particle physics *** is designed to operate in a center-of-mass energy range from 2 to 7 GeV with a peak luminosity of 0.5×10^(35) cm^(–2)·s^(–1) or *** STCF will produce a data sample about a factor of 100 larger than that of the presentτ-charm factory—the BEPCII,providing a unique platform for exploring the asymmetry of matter-antimatter(charge-parity violation),in-depth studies of the internal structure of hadrons and the nature of non-perturbative strong interactions,as well as searching for exotic hadrons and physics beyond the Standard *** STCF project in China is under development with an extensive R&D *** document presents the physics opportunities at the STCF,describes conceptual designs of the STCF detector system,and discusses future plans for detector R&D and physics case studies.

关键词： electron–positron collider tau-charm region high luminosity STCF detector conceptual design

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冠状动脉支架用316L和317L不锈钢低腐蚀率的测定

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生物医学工程学杂志 2006年第4期23卷 829-831页

作者：梁成浩郭亮陈婉大连海事大学机电与材料工程学院大连116026 大连理工大学化工学院大连116012

采用电化学恒电流线性极化法和原子吸收光谱法测定冠状动脉支架用316L和317L不锈钢在37℃人工模拟血浆T yrode's溶液中的低腐蚀率。结果表明,电化学恒电流线性极化法和原子吸收光谱法测得316L和317L不锈钢的腐蚀率分别为21×1-0 3、9.8... 详细信息

采用电化学恒电流线性极化法和原子吸收光谱法测定冠状动脉支架用316L和317L不锈钢在37℃人工模拟血浆T yrode's溶液中的低腐蚀率。结果表明,电化学恒电流线性极化法和原子吸收光谱法测得316L和317L不锈钢的腐蚀率分别为21×1-0 3、9.8×1-0 3μm/a和0.8×1-0 3、0.6×1-0 3μm/a,其值都远小于0.25μm/a,符合医用级金属标准,并且317L不锈钢的腐蚀率小于316L不锈钢。电化学恒电流线性极化法测得的金属腐蚀率大于原子吸收光谱法所得的结果。原子吸收光谱法测定结果能够准确反映金属材料溶解释放于溶液中的金属离子量。

关键词：冠状动脉支架低腐蚀率恒电流线性极化法原子吸收光谱法

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鸭疫里默氏菌噬菌体宿主谱的拓宽

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动物医学进展 2024年第5期45卷 34-38页

作者：程龙飞江南松刘荣昌傅秋玲梁齐章万春和黄瑜傅光华陈红梅福建省禽病防治重点实验室福建省畜禽疫病防治工程技术研究中心福建省畜禽疫病防控产业技术创新研究院福建省农业科学院畜牧兽医研究所福建福州350013

为建立一种拓宽鸭疫里默氏菌噬菌体噬菌谱的方法,将6株噬菌体混合液分别与6株宿主菌、18株多重耐药鸭疫里默氏菌(不能被6株噬菌体中的任何1株裂解)培养,收获培养液中的噬菌体,混合后同样进行30次传代。噬斑分析法测定30代混合液及混合... 详细信息

为建立一种拓宽鸭疫里默氏菌噬菌体噬菌谱的方法,将6株噬菌体混合液分别与6株宿主菌、18株多重耐药鸭疫里默氏菌(不能被6株噬菌体中的任何1株裂解)培养,收获培养液中的噬菌体,混合后同样进行30次传代。噬斑分析法测定30代混合液及混合液中单一噬菌体的噬菌谱。结果表明,30代噬菌体混合液可裂解79.2%(19/24)的鸭疫里默氏菌试验菌株;30代噬菌体混合液中分离出的单一噬菌体能裂解的试验菌株数量为2~9株;裂解数最多的5株单一噬菌体,以各自宿主菌传代10次,噬菌谱没有发生变化;将裂解谱互补的单一噬菌体混合,4株噬菌体即可裂解19株试验菌株,与30代噬菌体混合液相同。说明建立的方法可以拓宽噬菌体混合液、单一噬菌体的噬菌谱,为噬菌体鸡尾酒制剂的毒株选择奠定了基础。

关键词：鸭疫里默氏菌噬菌体噬菌谱拓宽

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Analytical solutions for constant-rate test in bounded confined aquifers with non-Darcian effect

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Journal of Groundwater Science and Engineering 2022年第4期10卷 311-321页

作者： Yi-jie Zong Li-hua chen Jian-jun Liu Yue-hui Liu Yong-xin Xu Fu-wan Gan liang Xiao College of Civil Engineering and Architecture Guangxi UniversityNanning 530004China Guangxi Key Laboratory of Disaster Prevention and Engineering Safety Guangxi UniversityNanning 530004China Key Laboratory of Disaster Prevention and Structural Safety of Ministry of Education Guangxi UniversityNanning 530004China Department of Earth Sciences University of the Western CapeCape Town 8000South Africa

This paper proposes a simplified analytical solution considering non-Darcian and wellbore storage effect to investigate the pumping flow in a confined aquifer with barrier and recharge *** mathematical modelling for the pumping-induced flow in aquifers with different boundaries is developed by employing image-well theory with the superposition principle,of which the non-Darcian effect is characterized by Izbash’s *** solutions are derived by Boltzmann and dimensionless ***,the non-Darcian effect and wellbore storage are especially investigated according to the proposed *** results show that the aquifer boundaries have non-negligible effects on pumping,and ignoring the wellbore storage can lead to an over-estimation of the drawdown in the first 10 minutes of *** higher the degree of non-Darcian,the smaller the drawdown.

关键词： Non-Darcian effect Bounded aquifer Wellbore storage Izbash’s equation Analytical solution

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Prevalence of deep venous thrombosis in patients with acute exacerbation of chronic obstructive pulmonary disease

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Chinese Medical Journal 2010年第12期123卷 1510-1514页

作者： DUAN Sheng-chen YANG Yuan-hua LI Xu-yan liang Xiao-ning GUO Rui-jun XIE wan-mu KUANG Tu-guang DAI hua-ping wang chen Beijing Institute of Respiratory Medicine Department of Respiratory and Critical Care Medicine Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Department of Ultrasonic Medicine Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China

Background Acute exacerbation of chronic obstructive pulmonary disease （COPD） is always associated with a high incidence and mortality. Because of the presence of some concomitant risk factors such as immobilization, bronchial superinfection, patients who are admitted for acute exacerbations of COPD are generally considered to be at moderate risk for the development of venous thromboembolism. In this study, we investigated the prevalence and the clinical manifestations of deep venous thrombosis （DVT） in patients with acute exacerbation of *** From March 2007 to March 2009, 520 consecutive patients were included in this study. On admission, color Doppler ultrasound of lower extremities in all cases was performed for diagnosing DVT. Patients with DVT were compared with those without DVT from such aspects as demographics, symptoms, physical signs and risk *** Among the 520 patients, DVT was found in 46 cases （9.7%）. In patients with DVT, the duration of hospitalization was longer （P=0.01）, and the mechanical ventilation requirement increased （P 〈0.001）. Other indicators for patients with more possibility of DVT were immobility exceeding 3 days （P 〈0.001）; pneumonia as concomitance （P=0.01）; respiratory failure type Ⅱ （P=0.013）; current smoking （P=0.001）. Lower extremity pain was more common in DVT cases in comparison to those without DVT （34.8% vs. 15.2%, P=0.01 ）.Conclusions The acute exacerbation of COPD patients, who were immobilized for over 3 days, complicated by pneumonia and had respiratory failure type Ⅱ, had a higher risk of DVT. In addition, DVT detection awareness should be increased in cases that had a lower extremity pain.

关键词： Chronic obstructive pulmonary disease deep venous thrombosis ultrasonography

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