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Development and pathogenicity analysis of full-length infectious cDNA clones of citrus yellow mottle-associated virus in citrus plants

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Journal of Integrative Agriculture 2023年第10期22卷 3034-3041页

作者： WU jia-xing ZHANG Song liang Xiao-fei xing Fei Sagheer ATTA WANG Xue-feng CAO Meng-ji National Citrus Engineering and Technology Research Center Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science CityCitrus Research InstituteSouthwest UniversityChongqing 400712P.R.China Guangxi Citrus Breeding and Cultivation Technology Innovation Center Guangxi Academy of Specialty CropsGuilin 541004P.R.China Department of Plant Protection Faculty of Agricultural SciencesGhazi UniversityDera Ghazi Khan 32200Pakistan

Citrus yellow mottle-associated virus(CiYMaV)belonging to the subgenus Mandarivirus within the genus Potexvirus,was first identified in 2018 from Pakistan(CiYMaV-PK),where it is endemic in several ***,three full-length cDNA clones(pCiYMaV-FL-1,pCiYMaV-FL-18,and pCiYMaV-FL-22)corresponding to the genomic RNA of CiYMaV were constructed and then agroinfiltrated on Chandler pummelo(Citrus grandis)seedlings using the vacuum infiltration *** the inoculated plants developed severe vein yellowing,leaf mottling,and dwarfing symptoms by 40 days post-infiltration(dpi).The results of a direct tissue blot immunoassay and reverse transcription polymerase chain reaction detection showed 94.7–100%infection rates of pCiYMaV-FL at 60 *** there being no observed difference among the three clones in the severity of symptom,pCiYMaV-FL-22 showed the highest accumulation levels of viral RNA and coat ***,pCiYMaV-FL-22 successfully infected seven other citrus varieties and induced symptoms in five of *** electron microscopy identified the presence of filamentous virus particles in extracts from systemic leaves of the plants infected with pCiYMaV-FL-22 at 6-months *** together,the results indicate that Koch's postulates were fulfilled for CiYMaV in citrus *** is the first report of full-length infectious cDNA clones of CiYMaV,and thus,the data provide a basis for further study of the molecular mechanisms of virus infection and the development of a viral vector to express foreign genes in citrus plants.

关键词： Mandarivirus vein yellow vacuum infiltration direct tissue blot immunoassay virus morphology

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A flexible carbon nanotube@V_(2)O_(5) film as a high-capacity and durable cathode for zinc ion batteries

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Journal of Energy Chemistry 2021年第8期30卷 126-133,I0004页

作者： Xiaowei Wang Liqun Wang Bao Zhang jianmin Feng jiafeng Zhang xing Ou Feng Hou ji liang School of Metallurgy and Environment Central South UniversityChangsha 410083China College of Physics and Materials Science Tianjin Normal University300387 TianjinChina Key Laboratory of Advanced Ceramics and Machining Technology of Ministry of Education School of Materials Science and EngineeringTianjin University300072 TianjinChina Institute for Superconducting&Electronic Materials(ISEM) Australian Institute of Innovative Materials(AIIM)Innovation CampusSquires WayUniversity of Wollongong2522 Australia

Aqueous zinc-ion batteries(ZIBs)are receiving a continuously increasing attention for mobile devices,especially for the flexible and wearable electronics,due to their non-toxicity,non-flammability,and low-cost *** the significant progress in achieving higher capacities for electrode materials of ZIBs,to endow them with high flexibility and economic feasibility is,however,still a significant challenge remaining ***,we present a highly flexible composite film composed of carbon nanotube film and V_(2)O_(5)(CNTF@V_(2)O_(5))with high strength and high conductivity,which is prepared by simply impregnating a porous CNT film with an aqueous V_(2)O_(5)sol under *** this material,intimate incorporation between V_(2)O_(5)and CNTs has been achieved,successfully integrating the high zinc ion storage capability with high mechanical *** a result,this CNTF@V_(2)O_(5)film delivers a high capacity of 356.6 m Ah g^(-1)at 0.4 A g^(-1)and excellent cycling stability with 80.1%capacity retention after 500 cycles at 2.0 A g^(-1).The novel strategy and the outstanding battery performance presented in this work should shed light on the development of high-performance and flexible ZIBs.

关键词： Zinc-ion batteries Carbon nanotube films Flexible cathode Wearable devices

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Spreading Speeds of Nonlocal KPP Equations in Heterogeneous Media

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Acta Mathematica Sinica,English Series 2022年第1期38卷 161-178页

作者： xing liang Tao ZHOU School of Mathematical Sciences and Wu Wen-Tsun Key Laboratory of Mathematics University of Science and Technology of ChinaHefei 230026P.R.China

This paper is devoted to studying the asymptotic behavior of the solution to nonlocal Fisher-KPP type reaction diffusion equations in heterogeneous *** kernel K is assumed to depend on the ***,we give an estimate of the upper and lower spreading speeds by generalized principal ***,we prove the existence of spreading speeds in the case where the media is periodic or almost periodic by showing that the upper and lower generalized principal eigenvalues are equal.

关键词： Nonlocal dispersal generalized principal eigenvalue spreading speed heterogeneous media homogenization

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Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第8期11卷 618-626页

作者： Kang-ting ji Jun-de CHAI Cheng xing jin-liang NAN Peng-lin YANG ji-fei TANG Department of Cardiology the Second Affiliated Hospital Wenzhou Medical College Wenzhou 325027 China

In this study, we examined the protective effects of Danshen both on endothelial progenitor cells （EPCs） in patients with hypercholesterolemia and on in-vitro EPCs of healthy volunteers. In the clinical study, we randomly divided 24 subjects with hypercholesterolemia into two groups （the control group and the Danshen-treated group）. At the end of two weeks of treatment, the EPC cellular functions of both groups were tested. The results indicated that, compared to the control group, EPCs in the Danshen-treated group showed significantly better cellular functions, which was manifested in the cloning number, the proliferation capacity, the number of EPC adhesions, and cell migration. In the subsequent in-vitro experiments, EPCs were treated with vehicle, oxidized low-density lipoprotein （Ox-LDL, 100 pg/ml）, or Ox-LDL （100 pg/ml） plus different concentrations of Danshen （Danshensu 2, 10, or 50 pg/ml, respectively） for 24 h. The results showed that Danshen treatments can prevent the detrimental effects of Ox-LDL on EPC cellular functions measured by proliferation capacity （0.24±0.08, 0.37±0.11, 0.30±0.04 vs. 0.13±0.02, P〈0.05, P〈0.01, and P〈0.01, respectively）, and adhesion ability （63.00_±11.60, 70.00±10.80, 85.50±11.41 vs. 40.50±6.85, all P〈0.01）. Compared to the group treated with Ox-LDL alone, Danshen treatment significantly decreased the lipid peroxidation end product malondialdehyde （MDA） [（4.34±0.54）, （3.98±0.47）, （3.46±0.31） vs. （5.57-±0.64） nmol/ml, all P〈0.01], increased the production of superoxide dismutase （SOD） [（29.74±0.71）, （31.09±0.83）, （30.41±0.65） vs. （14.76±3.99） U/ml, all P〈0.01], and lowered the expression of interleukin-6 （IL-6） [（24.62±7.69）, （27.04±3.14）, （33.38±18.86） vs. （230.67±33.53） pg/ml, all P〈0.01] and tumor necrosis factor-α （TNF-α） [（41.72±6.10）, （17.02±6.82）, （3.73±2.26） vs. （228.71±41.53） pg/ml, all P〈0.01] in Ox-LDL treated EPCs. These results suggest that Danshen may exert a protective effect through its antioxidant and anti-inflamm

关键词： Danshen Endothelial progenitor cells Oxidized low-density lipoprotein Hypercholesterolemia

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Physical Insight for Grafting Polymer Chains onto the Substrate via Computer Simulations:Kinetics and Property

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Chinese Journal of Polymer Science 2022年第7期40卷 817-833页

作者： Chi-Xin liang Hui Lu Bai-Ying Huang ji-Yuan xing Feng-Long Gu Hong Liu School of Chemistry South China Normal UniversityKey Laboratory of Theoretical Chemistry of Environment Ministry of EducationGuangzhou510006China State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical ChemistryCollege of ChemistryJilin UniversityChangchun130023China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China

Molecules adsorbed or attached on a surface is a quite basic phenomenon in numerous chemical or biological ***-onto is considered as a feasible way to achieve *** grafting reaction is essentially controlled by the diffusion of the molecules,thus it is more likely a physical issue,instead of a chemical *** of the experimental difficulty in measuring the properties of surface-attached molecules(e.g.,the polymeric molecules),the surface-bound molecules are often assumed as with the same properties as that of the start feeding ones in *** assumption was even used to guide further characterization,while it is proved to be invalid by different quantifying ***,an effective prediction for the properties of surface-bound molecules is still *** on a microscopic level and a dynamic perspective,the grafting process onto a flat substrate with polydisperse feeding polymeric molecules is investigated in-depth by coarse-grained Brownian dynamics simulation as well as model *** find from simulations that for the final grafting densityσ_(g)and the mean chain length of start feeding molecules,the dependence ofσ_(g)-^(γ)with the constant exponential factorγmay be a determined rule for one-end functionalized flexible linear polymer chains grafting on the flat *** grafting-onto is a multiple interplayed process,our simulation study indicates that there is an optimized initial concentration of start feeding molecules for achieving high grafting density of surface-bound *** also propose a correctional equation to quantitatively predict the molecular weight distribution(MWD)of surface-bound polymeric molecules,which may be effective for predicting the MWD of the surface-bound ones in specific *** simulation study helps to better understand the kinetics of grafting-onto process,and serves as a theoretical guide to achieve the precise design of surface modification materials vi

关键词： Grafting-onto Grafting density Polydisperse Molecular weight distribution

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A New Holographic Entropy Bound from Conformal Field Theory at the Killing Horizon

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Chinese Physics Letters 2002年第1期19卷 20-22页

作者： jing ji-liang Institute of Physics and Physics Department Hunan Normal University Changsha 410081

A new holographic entropy bound is obtained by using conformal field theory at the Killing horizon. The entropy bound is tighter than the well-known bounds, such as the Bekenstein, Bekenstein-Mayo and 'tHooft bounds. The result shows that the entropy of a system decreases when quantum effects are included. Therefore, the quantum effect will increase the degree of order of the system.

关键词： Holographic Entropy Conformal Field Theory Killing Horizon

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An Improved Higher-Order Time Integration Algorithm for Structural Dynamics

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Computer Modeling in Engineering & Sciences 2021年第2期126卷 549-575页

作者： Yi ji Yufeng xing Institute of Solid Mechanics Beihang UniversityBeijing100083China Shen Yuan Honors College Beihang UniversityBeijing100083China

Based on the weighted residual method,a single-step time integration algorithm with higher-order accuracy and unconditional stability has been proposed,which is superior to the second-order accurate algorithms in tracking long-term *** improving such a higher-order accurate algorithm,this paper proposes a two sub-step higher-order algorithm with unconditional stability and controllable *** the proposed algorithm,a time step interval[t_(k),t_(k)+h]where h stands for the size of a time step is divided into two sub-steps[t_(k),t_(k)+γh]and[t_(k)+γh,t_(k)+h].A non-dissipative fourth-order algorithm is used in the rst sub-step to ensure low-frequency accuracy and a dissipative third-order algorithm is employed in the second sub-step to lter out the contribution of high-frequency ***,two approaches are used to design the algorithm parameterγ.The rst approach determinesγby maximizing low-frequency accuracy and the other determinesγfor quickly damping out highfrequency *** present algorithm usesρ_(∞)to exactly control the degree of numerical dissipation,and it is third-order accurate when 0≤ρ_(∞)<1 and fourth-order accurate whenρ_(∞)=***,the proposed algorithm is self-starting and easy to *** illustrative linear and nonlinear examples are solved to check the performances of the proposed two sub-step higher-order algorithm.

关键词： Time integration algorithm two-sub-step higher-order accuracy controllable dissipation unconditional stability

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Analytical modeling and simulation of porous electrodes: Li-ion distribution and diffusion-induced stress

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Acta Mechanica Sinica 2018年第1期34卷 187-198页

作者： liang ji Zhansheng Guo Shanghai Institute of Applied Mathematics and Mechanics Shanghai University Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai University

A new model of porous electrodes based on the Gibbs free energy is developed, in which lithium-ion(Liion) diffusion, diffusion-induced stress(DIS), Butler–Volmer(BV) reaction kinetics, and size polydispersity of electrode particles are considered. The influence of BV reaction kinetics and concentration-dependent exchange current density(ECD) on concentration profile and DIS evolution are numerically investigated. BV reaction kinetics leads to a decrease in Li-ion concentration and DIS. In addition, concentrationdependent ECD results in a decrease in Li-ion concentration and an increase in DIS. Size polydispersity of electrode particles significantly affects the concentration profile and *** macroscopic state of charge(SOC) should consider the influence of the microscopic SOC values and mass fractions of differently sized particles.

关键词： New model of porous electrode Butler–Volmer reaction kinetics Size polydispersity Exchange current density Li-ion concentration distribution Diffusioninduced stress

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Study of Deuteron Separation Energy Based on Bayesian Neural Network Approach

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原子能科学技术 2023年第4期57卷 721-728页

作者： xing Kang liang Yan SUN Xiaojun College of Physics and Technology Guangxi Normal UniversityGuilin 541004China Guangxi Key Laboratory of Nuclear Physics and Technology Guangxi Normal UniversityGuilin 541004China

Deuteron separation energy is not only the basis for validating the nuclear mass models and nucleon-nucleon interaction potential,but also can determine the stability of a nuclide to certain *** neural network(BNN)approach,which has strong predictive power and can naturally give theoretical errors of predicted values,had been successfully applied to study the different kinds of separations except the deuteron *** this paper,several typical nuclear mass models,such as macroscopic model BW2,macroscopic-microscopic model WS4,and microscopic model HFB-31,are chosen to study the deuteron separation energy combining BNN *** root-mean-square deviations of these models are partly *** addition,the inclusion of physical parameters related to the pair and shell effects in the input layer can further improve the theoretical accuracy for the deuteron separation *** results show that the theoretical predictions are more reliable as more physical features of BNN approach are included.

关键词： Bayesian neural network deuteron separation energy pair and shell effects

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Comparative assessment and analysis of Rortex vortex in swirling jets

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Journal of Hydrodynamics 2019年第3期31卷 495-503页

作者： Nan Gui liang Ge Peng-xin Cheng xing-tuan Yang ji-yuan Tu Sheng-yao jiang Institute of Nuclear and New Energy Technology Collaborative Innovation Center of Advanced Nuclear Energy Technology Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education Tsinghua University Beijing 100084 China School of Engineering RMIT University Melbourne VIC 3083 Australia

Recently, a new definition, called Rortex, was proposed to quantify the purely rotational motion of fluids. In this work, based on the DNS data, the Rortex is used to assess and visualize the rotational motion and structure of the vortex in swirling jets in comparison with other kinds of vortex criteria, including Q , λ2,vorticity and Ω criteria. The Rortex vector,Ω, Q and criteria are found to be better than the vorticity criterion for the vortex core identification. The vector triangle formed by the Rortex R , the nonrotational shear S , and the vorticity R is analyzed to give mechanical explanations, especially of the effect of the non-rotational shear on the rotation of fluids. In addition, the probability density distributions (PDF) of the Rortex R , the nonrotational shear S, and the vorticity R are computed. The peak value of the PDF of the vorticity could be used to explain the pure rigid rotation effect and the combination effects of the rigid rotation and the non?rotational shear.

关键词： Swirling flow vortex Rortex direct numerical simulation rigid rotation non-rotational shear

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