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Comprehensive Assessment of the Safety of Eucommia ulmoides Leaf Extract for Consumption as a Traditional Chinese Health Food

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Journal of Renewable Materials 2023年第7期11卷 3091-3114页

作者： Huiling Fu Mijun Peng Qiuwen Tang haojun liang Yanli liang Jiali Fang Xuesong Wang School of Chemical and Environmental Engineering Hunan Institute of TechnologyHengyang421002China Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology Guangdong Provincial Engineering Research Center for Ambient Mass SpectrometryInstitute of AnalysisGuangdong Academy of Sciences(China National Analytical CenterGuangzhou)Guangzhou510070China

To ensure the export quality of Eucommia ulmoides leaf extract(ELE)and facilitate *** leaf inclusion in the directory of traditional Chinese health foods,an overall safety assessment of ELE was performed,including genotoxicity and long-term toxicity,according to the national food safety standards of *** variations in the reverse mutation number of the nominal bacterial strains were observed under ELE treatment in comparison with the solvent ***,the micronucleus rates of in vivo mammalian erythrocytes and in vitro mammalian cells under ELE treatment were equivalent to or significantly lower than those of the solvent *** fold change in the trifluorothymidine resistance mutation frequency of the thymidine kinase gene under ELE treatment was less than three times in comparison with the solvent control,suggesting that ELE did not cause ***,animal experiments showed that the growth performance of rats under ELE treatment was enhanced because the body weights of rats *** oxidative injury or inflammatory responses were induced and no histopathological lesions of tissues were detected under ELE *** addition,plasma triglycerides and low-density lipoprotein cholesterol levels significantly decreased,and plasma high-density lipoprotein cholesterol levels significantly increased with ELE treatment,suggesting that ELE was ***,moderate to excellent antimicrobial activities,a favorable anticancer capacity,and superior antioxidant abilities of ELE were found,implying ELE possesses good ***,we affirmed ELE is safe to consume as a traditional Chinese health food.

关键词： Eucommia ulmoides leaf extract genotoxicity long-term toxicity growth performance bioactivity

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Cascaded DNA circuits-programmed self-assembly of spherical nucleic acids for high signal amplification

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Science China Chemistry 2020年第1期63卷 92-98页

作者： Xiang Li Dongbao Yao Junxiang Zhou Xiang Zhou Xianbao Sun Bing Wei Chengxu Li Bin Zheng haojun liang Key Laboratory of Soft Matter Chemistry Chinese Academy of SciencesCollaborative Innovation Center of Chemistry for Energy MaterialsDepartment of Polymer Science and EngineeringHefei National Laboratory for Physical Sciences at the MicroscaleUniversity of Science and Technology of China School of Chemistry and Chemical Engineering Hefei Normal University

Signal amplification is an important issue in DNA nanotechnology and molecular *** this work,we report a strategy for the catalytic self-assembly of spherical nucleic acids(SNAs)programmed by two-layer cascaded DNA circuits through integrating an entropy-driven catalytic network,a catalytic hairpin assembly circuit,and a facile SNA assembly-based reporter *** integrated system could implement^100,000-fold signal amplification in the presence of 1 pM of input *** powerful amplification ability of nucleic acid signal,our strategy should be of great potential in fabricating more robust dynamic networks to be applied for signal transduction,DNA computing,and nucleic acid-based diagnostics.

关键词： catalytic self-assembly DNA circuit signal amplification spherical nucleic acids

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Effect of KBr on the micellar properties of CTAB

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Chinese Science Bulletin 2000年第20期45卷 1854-1857页

作者： ZHANG Weican LI Ganzuo MU Jianhai SHEN Qiang ZHENG Liqiang liang haojun WU Chi Key Laboratory for Colloid and Interface Chemistry of State Education Ministry Shandong UniversityJinan 250100China Open Laboratory for Bond Selective Chemistry Department of Chemical PhysicsUniversity of Science and Technology of ChinaHefei 230027China

The effect of KBr on the size, shape and microviscosity of CTAB micelles has been investigated by means of laser light scattering (LLS), 1H NMR measurements and fluorescence probe. The data obtained from the various techniques are quantitatively in agreement. The Rh of micelles in 0.01 mol ·L-1 CTAB solution increases from 3.5 nm to 43 nm and Rg increases to 89 nm with addition of KBr salt. In this process, both the microviscosity and molecular weight of micelles Mw have noticeable increases, too. The rod-like micelles are formed at 0.1 mol ·L -1 KBr and the worm-like micelles are formed at above 0.2 mol ·L-1 KBr.

关键词： molar weight of micelle radius of gyration hydrodynamic radius sphere-rod transition.

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Recent advances in molecular machines based on toehold-mediated strand displacement reaction

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Frontiers of Electrical and Electronic Engineering in China 2017年第1期5卷 25-41页

作者： Yijun Guo Bing Wei Shiyan Xiao Dongbao Yao Hui Li Huaguo Xu Tingjie Song Xiang Li haojun liang CAS Key Laboratory of Soft Matter Chemistry iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) Department of Polymer Science and Engineering University of Science and Technology of China Hefei 230026 China Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of China Hefei 230026 China College of Materials and Textile Engineering institute Jiaxing University Jiaxing 314000 China

Background： The DNA strand displacement reaction, which uses flexible and programmable DNA molecules as reaction components, is the basis of dynamic DNA nanotechnology, and has been widely used in the design of complex autonomous behaviors. Results： In this review, we first briefly introduce the concept of toehold-mediated strand displacement reaction and its kinetics regulation in pure solution. Thereafter, we review the recent progresses in DNA complex circuit, the assembly of AuNPs driven by DNA molecular machines, and the detection of single nucleotide polymorphism （SNP） using DNA toehold exchange probes in pure solution and in interface state. Lastly, the applications of toehold-mediated strand displacement in the genetic regulation and silencing through combining gene circuit with RNA interference systems are reviewed. Conclusions： The toehold-mediated strand displacement reaction makes DNA an excellent material for the fabrication of molecular machines and complex circuit, and may potentially be used in the disease diagnosis and the regulation of gene silencing in the near future.

关键词： toehold-mediated strand displacement DNA molecular machines SNP gene expression regulation

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本体柱状的线形嵌段共聚物在受限状态下的自组装结构

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高分子学报 2009年第4期19卷 298-308页

作者：陈鹏梁好均中国科学技术大学高分子科学与工程系合肥微尺度物质国家实验室安徽合肥230026 安徽大学化学化工学院安徽省绿色高分子材料重点实验室安徽合肥230039

综述了使用计算机模拟方法研究在本体状态下形成柱状结构的线形二嵌段和三嵌段共聚物在平行板间和纳米圆孔内的自组装结构.研究发现,嵌段共聚物体系在受限状态下自组装可以得到与本体状态下不同的纳米结构,调整受限状态的物理化学性质... 详细信息

综述了使用计算机模拟方法研究在本体状态下形成柱状结构的线形二嵌段和三嵌段共聚物在平行板间和纳米圆孔内的自组装结构.研究发现,嵌段共聚物体系在受限状态下自组装可以得到与本体状态下不同的纳米结构,调整受限状态的物理化学性质可以调控受限体系的相行为,从而诱导体系形成特定的结构.模拟研究还发现不同相分离强度和链结构的体系,在相同的受限状态下表现出不同的相行为.因此在制备纳米结构材料的研究中,人们要根据嵌段共聚物体系的特定性质,选择相应的受限环境,才能够实现有效的控制.

关键词：嵌段共聚物受限状态自组装计算机模拟

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Development and Characterization of Novel FAP-Targeted Theranostic Pairs: A Bench-to-Bedside Study

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Research 2024年第3期2023卷 473-485页

作者： Wei Huang Yizhen Pang Qiufang Liu Chenyi liang Shuxian An Qianyun Wu You Zhang Gang Huang haojun Chen Jianjun Liu Weijun Wei Department of Nuclear Medicine Institute of Clinical Nuclear MedicineRenj HospitalSchool of Medicine Shanghai Jiao Tong UniversityShanghai 200127China Department of Nuclear Medicine and Minnan PET Center Xiamen Cancer CenterXiamen Key Laboratory of Radiation OncologyThe First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361003China Department of Nuclear Medicine Fudan University Shanghai Cancer CenterFudan UniversityShanghai 200032China

Fibroblast activation protein (FAP) is among the most popular targets in nuclear medicine imaging and cancer theranostics. Several small-molecule moieties (FAPI-04, FAPI-46, etc.) are used for developing FAP-targeted theranostic agents. Nonetheless, the circulation time of FAP inhibitors is relatively short, resulting in rapid clearance via kidneys, low tumor uptake, and associated unsatisfactory treatment efficacy. To address the existing drawbacks, we engineered 3 peptides named FD1, FD2, and FD3 with different circulation times through solid-phase peptide synthesis. All the 3 reported peptides bind to human and murine FAP with single-digit nanomolar affinity measured by surface plasmon resonance. The diagnostic and therapeutic potential of the agents labeled with 68Ga and 177Lu was assessed in several tumor models exhibiting different levels of FAP expression. While radiolabeled FD1 was rapidly excreted from kidneys, radiolabeled FD2/FD3 have significantly prolonged circulation, increased tumor uptake, and decreased kidney accumulation. Our findings indicated that [68Ga]Ga-DOTA-FD1 positron emission tomography (PET) effectively detected FAP dynamics, whereas [177Lu]Lu-DOTA-FD2 and [177Lu]Lu-DOTA-FD3 exhibited remarkable therapeutic efficacy in FAP-overexpressing tumor models, including pancreatic cancer cell models characterized by abundant stroma. Moreover, a pilot translational investigation demonstrated that [68Ga]Ga-DOTA-FD1 had the capability to identify both primary and metastatic tumors with precision and distinction. In summary, we developed [68Ga]Ga-DOTA-FD1 for same-day PET imaging of FAP dynamics and [177Lu]Lu-DOTA-FD2 and [177Lu]Lu-DOTA-FD3 for effective radioligand therapy of FAP-overexpressing tumors.

关键词： DOTA expressing labeled

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流场驱动高分子链迁移穿过微通道的耗散粒子动力学模拟

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高分子学报 2012年第2期22卷 160-167页

作者：郭佳意李学进梁好均中国科学院软物质化学重点实验室中国科学技术大学高分子科学与工程系合肥230026

利用耗散粒子动力学模拟方法研究了高分子链在流场驱动作用下迁移穿过微通道过程中的链构象变化和动力学行为.在足够大的流场力驱动作用下,高分子链在沿着流场方向逐渐被拉伸,从而能够穿过管径小于其自身尺寸的微通道.耗散粒子动力学模... 详细信息

利用耗散粒子动力学模拟方法研究了高分子链在流场驱动作用下迁移穿过微通道过程中的链构象变化和动力学行为.在足够大的流场力驱动作用下,高分子链在沿着流场方向逐渐被拉伸,从而能够穿过管径小于其自身尺寸的微通道.耗散粒子动力学模拟结果表明高分子链的迁移过程主要分为3个步骤:(1)在流场驱动作用下,高分子链漂移并逐渐靠近微通道入口;(2)高分子链逐渐调整自身构象,并使其部分进入微通道;(3)高分子链成功穿过微通道.同时,模拟还发现当高分子链尺寸大于微通道细管道管径时,高分子链穿过微通道所需的平均迁移时间随着流量的增加而逐渐减小.此外,为了研究高分子链刚性对高分子链穿过微通道的影响,模型中还引入了蠕虫状高分子链模型.模拟结果发现,高分子链的链刚性越强,其迁移穿过微通道的时间越长.

关键词：高分子链迁移流场驱动力跨膜输运耗散粒子动力学

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缩短shRNA的3′尾与靶标mRNA的配对降低脱靶效应

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功能高分子学报 2023年第3期36卷 310-320页

作者：尹雪姚东宝梁好均中国科学技术大学高分子科学与工程系中国科学院软物质化学重点实验室合肥微尺度物质科学国家研究中心能源材料化学协同创新中心

基于对microRNA和短发夹RNA(shRNA)的3′尾功能的理解，提出了一种仅通过缩短shRNA的3′尾与靶标序列的互补长度来降低脱靶效应的方法。此方法可以在不损伤shRNA基因沉默效率的前提下达到降低shRNA脱靶效应的目的，从而有效提高shRNA的... 详细信息

基于对microRNA和短发夹RNA(shRNA)的3′尾功能的理解，提出了一种仅通过缩短shRNA的3′尾与靶标序列的互补长度来降低脱靶效应的方法。此方法可以在不损伤shRNA基因沉默效率的前提下达到降低shRNA脱靶效应的目的，从而有效提高shRNA的基因沉默特异性。此策略不受反义链3′区域序列的限制，可以显著改进RNA干扰设计的规则，一定程度上简化shRNA药物设计中的序列限制，拓宽其作为治疗和诊断工具在医疗中的用途和前景。

关键词：短发夹RNA RNA干扰基因沉默 3′尾脱靶效应

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DNA:结构、链替换反应和反应网络

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中国科学：化学 2023年第4期53卷 721-733页

作者：肖石燕李成勖梁好均中国科学技术大学化学与材料科学学院精准智能化学重点实验室合肥230026

DNA不仅是生物遗传信息的载体,更是一种可精确操控和设计、高度生物相容的高分子材料.由于DNA的特异性碱基互补配对和碱基序列的可编程性,人们设计了DNA链替换反应.基于链替换反应,可以构建链替换反应网络,实现特定的功能.本文综述了近... 详细信息

DNA不仅是生物遗传信息的载体,更是一种可精确操控和设计、高度生物相容的高分子材料.由于DNA的特异性碱基互补配对和碱基序列的可编程性,人们设计了DNA链替换反应.基于链替换反应,可以构建链替换反应网络,实现特定的功能.本文综述了近年来在DNA结构和力学性质、链替换反应的微观机制和速率调控机制、反应网络构建和调控,及其在球形核酸自组装和细胞组装等方面的研究进展.最后,我们展望了DNA链替换反应网络未来研究和发展方向.

关键词： DNA DNA力学性质链替换反应反应网络自组装细胞组装

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Toehold 开关调控的大肠杆菌基因表达体系

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功能高分子学报 2023年第3期36卷 302-309页

作者：李成勖肖石燕梁好均中国科学技术大学高分子科学与工程系中科院软物质化学重点实验室合肥230026

设计了一种核糖体调节器—“立足点开关”(toehold switch)。与传统核糖体调节器设计不同的是,该核糖体调节器的起始密码子(AUG)和核糖体结合位点位于核糖体调节器中发夹结构RNA的环(loop)上,而“茎”(stem)结构是完全互补配对的RNA双... 详细信息

设计了一种核糖体调节器—“立足点开关”(toehold switch)。与传统核糖体调节器设计不同的是,该核糖体调节器的起始密码子(AUG)和核糖体结合位点位于核糖体调节器中发夹结构RNA的环(loop)上,而“茎”(stem)结构是完全互补配对的RNA双链。通过RNA链替换反应,引发链(trigger)RNA能够打开发夹结构RNA,从而激活下游绿色荧光蛋白的表达,导致荧光信号的增长,最终实现对大肠杆菌基因表达的调控。系统研究了“茎”的长度对绿色荧光蛋白表达的调控作用。实验结果表明,当“茎”的长度大于8个碱基时,发夹结构RNA就能有效地抑制绿色荧光蛋白的表达。进一步的共表达实验结果表明,引发链RNA能够打开发夹RNA,从而调控大肠杆菌基因表达。Toehold开关调控的大肠杆菌基因表达系统具有可拓展性,可应用于多基因表达调控,对基因疾病诊疗具有潜在应用价值。

关键词：核糖体调节器立足点开关发夹RNA 茎 RNA链替换反应

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