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Chinese Chemical Letters 1991年第7期2卷 521-522页

作者： Jian Guo MA Tong Shuang li yu lin li Laboratory of Applied Organic Chemistry,Lanzhou University,Lanzhou 730000

The synthesis of 4,5-secocholestane and 4-methyl-4,5-secocholestane from cholesterol are reported.

关键词： ppm SYNTHESIS OF 4,5-SECOCHOLESTANE AND 4-METHYL-4,5-SECOCHOLESTANE

Mesenchymal stem cells transduced by PLEGFP-N1 retroviral vector maintain their biological features and differentiation

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Chinese Medical Journal 2005年第20期118卷 1728-1734页

作者： HE Xu li yu-lin WANG Xin-rui GUO Xin NIU yun Key Laboratory of Pathobiology Ministry of Education JilinUniversity Changchun 130021 China Medical Center Hong Kong University of Science and TechnologyPeking University Shenzhen 518000 China

Background Enhanced green fluorescent protein （EGFP） has been an important reporter gene for gene therapy. Human mesenchymal stem cells （hMSCs） are ideal target cells in cell transplantation and tissue engineering. We investigated their biological characteristics and differentiation mediated by PLEGFP-N1 retroviral transduction.Methods hMSCs were isolated from human bone marrow by density gradient fractionation and adherence to plastic flasks. Individual colonies were selected and cultured in tissue dishes. Packaging cells PT67 were transfected by PLEGFP-N1 retroviral vector , and hMSCs were transduced by viral supernatant infection. Meanwhile, hMSCs-EGFP were identified by immune phenotypes and whether it could differentiate into osteoblasts or adipocytes under conditioned media was investigated.Results The rate of stably transduced hMSCs-EGFP was up to 96% after being screened by G418. hMSCs-EGFP exhibited fibroblast-like morphological features. Flow cytometric analyses showed that hMSCs-EGFP were positive for CD73, CD105, CD166, CD90 and CD44, but negative for CD34 and CD45. In addition, it could functionally be induced into osteocytes or adipocytes under conditioned media. These biological features of hMSCs-EGFP were consistent with those of hMSCs.Conclusions hMSCs transduced by PLEGFP-N1 retroviral vector can be used in vivo securely because they can maintain their biological characteristics and differentiation. It is a simple and reliable way to trace the changes of hMSCs in vivo by EGFP during cell transplantation and gene therapy.

关键词： mesenchymal stem cells·bone marrow·enhanced green fluorescent protein·retroviral vector· gene therapy

6-Oxodendrolasinolide的全合成

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高等学校化学学报 2007年第7期28卷 1292-1296页

作者：李毅张涛李裕林兰州大学有机化学研究所功能有机分子化学国家重点实验室兰州730000 杭州民生药业集团绍兴制药有限公司绍兴312071

以香叶醇(2)和异戊烯醇(5)为起始原料,经过12步反应,以5·6%的总收率完成了倍半萜内酯6-Oxodendrolasinolide(1)的全合成.其关键步骤包括乙烯基二噻烷(7)的负离子与烯丙基氯(4a)的区域选择性烷基化反应和Corey′s氧化内酯化反应.

关键词：全合成丁烯酸内酯倍半萜区域选择性烷基化

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基于深度学习模型的图像质量评价方法

华中科技大学学报（自然科学版） 2016年第12期44卷 70-75页

作者：李琳余胜生华中科技大学计算机科学与技术学院湖北武汉430074 武汉理工大学计算机科学与技术学院湖北武汉430070

为了有效提取与视觉感知质量高度相关的图像特征,改进图像质量评价方法,在深度学习的框架下,提出一个全新的卷积神经网络IQF-CNN结构,能自动学习判别性更强的图像质量特征,并利用学习的特征进行图像质量评价.同时,该算法采用局部亮度系... 详细信息

为了有效提取与视觉感知质量高度相关的图像特征,改进图像质量评价方法,在深度学习的框架下,提出一个全新的卷积神经网络IQF-CNN结构,能自动学习判别性更强的图像质量特征,并利用学习的特征进行图像质量评价.同时,该算法采用局部亮度系数归一化、dropout等技术进一步提高网络学习能力.实验结果表明:该算法能较准确地评估五种常用的图像失真,尤其在JPEG压缩、JPEG2000压缩和高斯模糊图像失真上与人眼主观感知质量具有很高的一致性,整体性能比较优于其他经典评价方法.

关键词：深度学习卷积神经网络特征学习无参考图像质量评价归一化

SYNTHETIC STUDIES ON 9-HYDROXY-CEMBRENE-C

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Chinese Chemical Letters 1993年第9期4卷 769-770页

作者： ling li Wei Dong li yu lin li State Key Laboratory of Organic Chemistry,Institute of Organic Chemistry,Lanzhou University,Lanzhou 730000

A dicarbonyl precursor of 9-hydroxy-cembrene-C,8-tetrahydropyranoxy-3,7,11,15- tetramethyl-14-oxo-2E,6E,10E-hexdecatrienal,was prepared from farnesyl acetate via four steps.

关键词： ppm CO li SYNTHETIC STUDIES ON 9-HYDROXY-CEMBRENE-C

Kinematical analysis of highway tunnel collapse using nonlinear failure criterion

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Journal of Central South University 2014年第1期21卷 381-386页

作者：杨小礼杨子汉潘秋景李育林 School of Civil Engineering Central South University

For different kinds of rocks,the collapse range of tunnel was studied in the previously published ***,some tunnels were buried in soils,and test data showed that the strength envelopes of the soils followed power-law failure *** this work,deep buried highway tunnel with large section was taken as objective,and the basic expressions of collapse shape and region were deduced for the highway tunnels in soils,based on kinematical approach and power-law failure *** order to see the effectiveness of the proposed expressions,the solutions presented in this work agree well with previous results if the nonlinear failure criterion is reduced to a linear Mohr-Coulomb failure *** present results are compared with practical projects and tunnel design *** numerical results show that the height and width of tunnel collapse are greatly affected by the nonlinear criterion for the tunnel in soil.

关键词： upper bound theorem tunnel collapse highway tunnel nonlinear failure criterion

Advances in the Development of Gradient Scaffolds Made of Nano‑Micromaterials for Musculoskeletal Tissue Regeneration

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Nano-Micro Letters 2025年第3期17卷 455-500页

作者： Lei Fang Xiaoqi lin Ruian Xu Lu liu yu Zhang Feng Tian Jiao Jiao li Jiajia Xue Beijing Laboratory of Biomedical Materials Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China School of Biomedical Engineering Faculty of Engineering and ITUniversity of Technology SydneySydneyNSW 2007Australia

The intricate hierarchical structure of musculoskeletal tissues,including bone and interface tissues,necessitates the use of complex scaffold designs and material structures to serve as tissue-engineered *** has led to growing interest in the development of gradient bone scaffolds with hierarchical structures mimicking the extracellular matrix of native tissues to achieve improved therapeutic *** on the anatomical characteristics of bone and interfacial tissues,this review provides a summary of current strategies used to design and fabricate biomimetic gradient scaffolds for repairing musculoskeletal tissues,specifically focusing on methods used to construct compositional and structural gradients within the *** latest applications of gradient scaffolds for the regeneration of bone,osteochondral,and tendon-to-bone interfaces are ***,the current progress of testing gradient scaffolds in physiologically relevant animal models of skeletal repair is discussed,as well as the challenges and prospects of moving these scaffolds into clinical application for treating musculoskeletal injuries.

关键词： Gradient scaffolds Musculoskeletal tissues Advanced manufacturing Biomaterials Tissue regeneration

THE SYNTHETIC ATTEMPT OF(±)-MALABARIC ACID AND(±)-ANISOMELOL——SYNTHE S IS OF PRECURSORS FOR(+)-MALABARIC ACID AND(±)-ANIOSMELOL

维普期刊数据库评论

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Chinese Chemical Letters 1992年第1期3卷 15-18页

作者： Ying li Wei Dong li yu lin li State Key Laboratory of Applied Organic Chemistry and Institute of Organic Chemistry,Lanzhou University,Lanzhou 730000

The precursors I(R′=CH_2OTHP,or CO_2Et)for(±)-Malabaric acid(1)and(±)- Anisomelol(2)were synthesized from geraniol 3 and homogeranyl iodide 5(2,6-dimetyl-9-iodo-2,6E- nonadiene)via Wadsworth-Emmons condensation and... 详细信息

关键词： ACID ppm ANIOSMELOL ANISOMELOL MALABARIC ACID AND SYNTHE S IS OF PRECURSORS FOR THE SYNTHETIC ATTEMPT OF

Association of therapeutic occasion, gross motor function grading and developmental level with gross motor functional recovery in children with cerebral palsy

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Neural Regeneration Research 2007年第9期2卷 548-551页

作者： Hongying li Huafeng yu lin Sang Haixia Ma Rehabifitation Center Qilu Children＇s Hospital of Shandong University Ji＇nan 250022 Shandong Province China

BACKGROUND： The abnormal posture and motor pattern have not stabilized in children with cerebral palsy at early period, thus timely treatment can establish normal postural reflex and motor pattern, and prevent complications of muscle contracture, ankylosis, skeletal deformity, etc. The clinical factors affecting the rehabilitative efficacy of gross motor function in children with cerebral palsy should be observed. OBJECTIVE： To observe the effects of therapeutic occasion, grading of gross motor function and developmental level on the rehabilitative efficacy in children with cerebral palsy. DESIGN： A case-controlled analysis. SETTING： Qilu Children＇s Hospital of Shandong University. PARTICIPANTS： Totally 138 children with cerebral palsy, who were hospitalized for 12 months in the Rehabilitation Center of Qilu Children＇s Hospital, Shandong University, were selected from April 2004 to September 2006, and all the children were diagnosed to be accorded with the standard set by the national seminar on cerebral palsy in 2004. There were 97 males and 41 females, including 55 cases of 0-2 years old, 47 cases of 2-4 years and 36 cases of 4-6 years. Informed contents were obtained from relatives of all the children. METHODS： ① Comprehensive rehabilitation treatment： Vojta method was to induce the children to turn over the body and crawl by stimulating reflective turn over and crawling on belly. Bobath method including trainings of head control, turning over body, keeping sitting position, keeping balance, crawling, keeping standing position, and walking, etc.; The children were massaged by using the maneuvers of push, press, rub, pull, wave, etc. according to the sites and types of palsy. Acupuncture was performed mainly at bilateral motor areas, the needle was retained for 1 hour per time, 6 days continuously every week, and followed by a 1-day interval. ② Prognosis assessment： The gross motor functional grading of the children with cerebral palsy at admission was asses

关键词： cerebral palsy age started to accept treatment developmental level rehabilitation

Heme oxygenase-1 promotes Caco-2 cell proliferation and migration by targeting CTNND1

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Chinese Medical Journal 2013年第16期126卷 3057-3063页

作者： ZHANG li liU yu-lin CHEN Guang-xiang CUI Bin WANG Jin-shen SHI yu-long li Le-ping GUO Xiao-bo Department of Gastrointestinal Surgery Provincial Hospital Affiliatedto Shandong University Jinan Shandong 250021 China

Background Heme oxygenase-1 （HO-1） can be induced by inflammatory cytokines,oxidation,ischemia,hypoxia,and *** a ＂graft survival protective gene,＂ HO-1 is a hot spot in organ transplantation ***,the role of HO-1 gene expression in the function of human colon adenocarcinoma cell line （Caco-2） cells has not been reported *** The role of HO-1 in the proliferation and migration of Caco-2 cells was analyzed using a stable HO-1 expression *** constructed a recombinant adeno-associated virus plasmid containing the HO-1 gene,heme oxygenase 1 （HMOX1）,which was transfected into Caco-2 intestinal *** identified a number of target genes by global microarray analysis combined with real-time polymerase chain reaction （PCR） and chromatin immunoprecipitation *** Our results showed that significant HO-1 upregulation was demonstrated in the Caco-2 cells after HO-1 *** of HO-1 expression promoted proliferation and invasion in *** CTNND1 gene,a member of the armadillo protein family,was identified as a direct HO-1 target *** Overexpression of HO-1 promotes Caco-2 cell proliferation and migration by targeting the CTNND1 gene.

关键词： heme oxygenase-1 Caco-2 cells microarray CTNND1