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Transformation of plant young proembryos by electroporation

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Chinese Science Bulletin 2000年第13期45卷 1202-1206,1249页

作者： shitao li Shenghua Wang Hongyuan Yang Wuhan Univ Res Ctr Dev Biol Wuhan 430072 Peoples R China

It is first reported that plant young proembryos expressed exogenous reporter genes by electroporation. Young proembryos with 8-32 cells and globular proembryos with 250-400 cells could be isolated by enzymatic maceration combined with microdissection. After electroporation with GUS or GFP genes, the proembryos were cultured for 1 -2 d in KM8p medium. At the field strength of electroporation 500-1 500 V/cm, blue reaction of GUS or green fluorescence of GFP could be observed in the proembryos. The highest transient expression frequency of young proembryos (2.2%) was obtained at the field strength of 750 V/cm, whereas the highest frequency of globular proembryos (5.9%) was obtained at the field strength of 1 250 V/cm. Taking the proportion of transformed cells in the whole cells of proembryos as efficient transformation frequency, the efficient transformation frequency of the young proembryos was 7 times that of the globular proembryos.

关键词： electroporation GUS GFP proembryo Nicotiana tabacum.

Microstructure and properties of indium tin oxide thin films deposited by RF-magnetron sputtering

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Rare Metals 2006年第4期25卷 359-364页

作者： li shitao QIAO Xueliang CHEN Jianguo JIA Fang WU Changle State Key Laboratory of Plastic Forming Simulation and Die ＆ Mould Technology Huazhong University of Science and Technology Wuhan 430074China

Tin-doped indium oxide （ITO） thin films were prepared using conventional radio frequency （RF） planar magnetron sputtering equipped with IR irradiation using a ceramic target of In2O3/SnO2 with a mass ratio of 1：1 at various IR irradiation temperatures T1 （from room temperature to 400℃）. The refractive index, deposited ratio, and resistivity are functions of the sputtering Ar gas pressure. The microstructure of ITO thin films is related to IR T1, the crystalline seeds appear at T1= 300℃, and the films are amorphous at the temperature ranging from 27℃ to 400℃. AFM investigation shows that the roughness value of peak-valley of ITO thin film （Rp-v） and the surface microstructure of rio thin films have a close relation with T1. The IR irradiation results in a widening value of band-gap energy due to Burstein-Moss effect and the maximum visible transmittance shifts toward a shorter wavelength along with a decrease in the film＇s refractive index. The plasma wavelength and the refractive index of ITO thin films are relative to the T1. XPS investigation shows that the photoelectrolytic properties can be deteriorated by the sub-oxides. The deterioration can be decreased by increasing the oxygen flow rote （fo2）, and the mole ratio of Sn/In in the samples reduces with an increase info2.

关键词： indium-tin oxide (ITO) photoelectrolytic properties RF-magnetron sputtering IR irradiation temperature microstructure refractive index

TRIM65 E3 ligase targets VCAM-1 degradation to limit LPS-induced lung inflammation

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Journal of Molecular Cell Biology 2020年第3期12卷 190-201页

作者： Yong li Xuan Huang Fang Guo Tianhua Lei shitao li Paula Monaghan-Nichols Zhisheng jiang Hong-Bo Xin Mingui Fu Department of Biomedical Science School of MedicineUniversity of Missouri-Kansas CityKansas CityMO 64108USA Institute of Translational Medicine Nanchang UniversityNanchang 330031China Institute of Cardiovascular Diseases Department of PathophysiologyHengyang Medical CollegeUniversity of South ChinaHengyang A21001China Department of Physiological Sciences Center for Veterinary and Health sciencesOklahoma State UniversityStillwaterOK 7A078USA

Although the adhesion molecules-mediated leukocyte adherence and infiltration into tissues is an important step of inflammation,the post-translational regulation of these proteins on the endothelial cells is poorly ***,we report that TRIM65,an ubiquitin E3 iigase of tripartite protein family,selectively targets vascular cell adhesion molecule 1(VCAM-1)and promotes its ubiq-uitination and degradation,by which it critically controls the duration and magnitude of sepsis-induced pulmonary ***65 is constitutively expressed in human vascular endothelial *** TNFa-induced endothelial activation,the protein levels ofTRIM65 and VCAM-1 are inversely *** of wild-type TRIM65,but not expression of aTRIM65 mutant that lacks E3 ubiquitin ligase function in endothelial cells,promotes VCAM-1 ubiquitination and degradation,whereas small interference RNA-mediated knockdown of TRIM65 attenuates VCAM-1 protein *** experiments show that TRIM65 directly interacts with VCAM-1 protein and directs its polyubiquitination,by which TRIM65 controls monocyte adherence and infiltration into tissues during ***,TRIM65-deficient mice are more sensitive to lipopolysaccharide-induced death,due to sustained and severe pulmonary *** together,our studies suggest that TRIM65-mediated degradation of VCAM-1 represents a potential mechanism that controls the duration and magnitude of infiammation.

关键词： TRIM65 VCAM-1 endothelial activation lung inflammation ubiquitination

Non-proteolytic ubiquitination of OTUliN regulates NF-κB signaling pathway

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Journal of Molecular Cell Biology 2020年第3期12卷 163-175页

作者： Mengmeng Zhao Kun Song Wenzhuo Hao lingyan Wang Girish Patil Qingmei li lingling Xu Fang Hua Bishi Fu Jens C.Schwamborn Martin E.Dorf shitao li Department of Physiological Sciences Center for Veterinary Health SciencesOklahoma State UniversityStillwaterOK 74078USA Key Laboratory of Animal Immunology Henan Academy of Agricultural SciencesZhengzhouChina State Key Laboratory of Virology Medical Research InstituteWuhan UniversityWuhanChina Luxembourg Centre for Systems Biomedicine University of LuxembourgLuxembourg CityLuxembourg Department of Immunology Harvard Medical SchoolBostonMA 02115USA f These authors contributed equally to this work

NF-κB signaling regulates diverse processes such as cell death,inflammation,immunity,and *** activity of NF-κB is controlled by methionine 1-linked linear polyubiquitin,which is assembled by the linear ubiquitin chain assembly complex(LUBAC)and the ubiquitin-conjugating enzyme *** studies found that the deubiquitinase OTUliN breaks the linear ubiquitin chain,thus inhibiting NF-κB *** the essential role of OTUliN in NF-κB signaling has been established,the regulatory mechanism for OTUliN is not well *** discover the potential regulators of OTUliN,we analyzed the OTUliN protein complex by proteomics and revealed several OTUliN-binding proteins,including LUBAC and tripartite motif-containing protein 32(TRIM32).TRIM32 is known to activate NF-κB signaling,but the mechanism is not *** complement experiments found that TRIM32 is upstream of OTUliN and TRIM32-mediated NF-κB activation is dependent on *** of the E3 ligase domain showed that the E3 ligase activity is essential for TRIM32-mediated NF-κB *** experiments found that TRIM32 conjugates polyubiquitin onto OTUliN and the polyubiquitin blocks the interaction between HOIP and OTUliN,thereby activating NF-κB *** together,we report a novel regulatory mechanism by which TRIM32-mediated non-proteolytic ubiquitination of OTUliN impedes the access of OTUliN to the LUBAC and promotes NF-κB activation.

关键词： NF-κB LUBAC TNF linear ubiquitination TRIM proteomics

Enhancement of the angular rotation measurement sensitivity based on SU(2)and SU(1,1) interferometers

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Photonics Research 2017年第6期5卷 136-141页

作者： JUN liU WENXIAO liU shitao li DONG WEI HONG GAO FUli li Key Laboratory of Quantum Information and Quantum Optoelectronic Devices of Shaanxi Province School of ScienceXi'an Jiaotong University

We investigate the sensitivity of the angular rotation measurement with the method of homodyne detection in SU(2) and SU(1,1) interferometers by employing orbital angular momentum(OAM). By combining a coherent beam with a vacuum beam in an SU(2) interferometer, we get the sensitivity of the angular rotation measurement as 1/(2N^(1/2)l). We can surpass the limit of the angular rotation measurement in an SU(1,1) interferometer by combining a coherent beam with a vacuum beam or a squeezed vacuum beam when the probe beam has OAM. Without injection, the sensitivity can reach 1/(2N^(1/2)l). In addition, by employing another construction of an SU(1,1) interferometer where the pump beam has OAM, with the same injection of an SU(1,1) interferometer, the sensitivity of the angular rotation measurement can be improved by a factor of 2, reaching 1/(4Nl). The results confirm the potential of this technology for precision measurements in angular rotation measurements.

关键词： OAM interferometers Enhancement of the angular rotation measurement sensitivity based on SU and SU

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试析“非物质文化遗产”的基本特点与性质

广西民族研究 2007年第3期 182-188页

作者：李世涛中国艺术研究院副研究员北京100029

非物质文化遗产与自然遗产、世界文化遗产、传统民族民间文化遗产、文化景观遗产既有联系,又有区别。论者主要从非物质文化遗产与其他类型遗产的区别中,提出并分析了非物质文化遗产的独特性、活态性、传承性、变异性、综合性、民族性和... 详细信息

非物质文化遗产与自然遗产、世界文化遗产、传统民族民间文化遗产、文化景观遗产既有联系,又有区别。论者主要从非物质文化遗产与其他类型遗产的区别中,提出并分析了非物质文化遗产的独特性、活态性、传承性、变异性、综合性、民族性和地域性等基本特点,以及其他特点。同时,论者还结合中国的国情分析了非物质文化遗产的性质,并建议据此制定相关政策、进行保护非物质文化遗产的实践活动。

关键词：区别基本特点性质

重构时空的政治维度——詹姆逊后现代美学中的时空理论

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甘肃社会科学 2007年第2期 146-151页

作者：李世涛中国艺术研究院北京100029

詹姆逊的后现代时空论是其后现代美学的有机组成部分,他揭示了后现代社会中时空意识的变化,尤其空间急剧扩张所导致的“超空间”的形成,及其对人的日常体验、历史意识和文化的消极影响。这些困境促使詹姆逊提出了“认知测绘”的设想,希... 详细信息

詹姆逊的后现代时空论是其后现代美学的有机组成部分,他揭示了后现代社会中时空意识的变化,尤其空间急剧扩张所导致的“超空间”的形成,及其对人的日常体验、历史意识和文化的消极影响。这些困境促使詹姆逊提出了“认知测绘”的设想,希望借助于心理、想象等因素来克服后现代主体的瘫痪,并通过开掘时空的政治维度以重塑强有力的政治主体。詹姆逊敏锐地抓住了后现代时空的新变化及其影响,致力于发觉时空的政治潜能,拓展了马克思主义的有关论述。但过于夸大文化、乌托邦的作用,有新马克思主义“文化决定论”的弊病,在整合马克思主义和各种西方后学中也产生了难以克服的矛盾。

关键词：后现代超空间认知测绘政治抵抗

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认同的现代之维:泰勒对现代认同的研究

外国文学 2023年第4期 97-106页

作者：李世涛北京外国语大学中文学院北京100089

泰勒的研究表明,认同在现代社会中发挥着巨大的作用,自我认同是现代认同的核心;现代认同经历了一个历史的发展过程,不但深刻地影响了个体的体验、意义感、价值,而且还对个体的选择、生活模式与现代社会产生了巨大的影响。现代认同与现... 详细信息

泰勒的研究表明,认同在现代社会中发挥着巨大的作用,自我认同是现代认同的核心;现代认同经历了一个历史的发展过程,不但深刻地影响了个体的体验、意义感、价值,而且还对个体的选择、生活模式与现代社会产生了巨大的影响。现代认同与现代人、现代生活的实践是一种复杂的互动关系。泰勒从“自我”介入研究,梳理了自我形成的两条线索,即“分解自我”式的线索和“自我探索模式”的线索,进而分析了自我理解的问题。他的研究能够推动我们深入地理解西方的现代认同、精神、观念、自我、审美、现代性和审美现代性等议题。

关键词：现代认同现代性分期生活模式

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朱光潜马克思主义理论和美学的翻译研究活动

甘肃社会科学 2021年第6期 18-26页

作者：李世涛北京外国语大学中文学院北京100089

中华人民共和国成立以后,朱光潜开始学习马列主义并以此指导其学术研究,他不但为建立中国的马克思主义美学、文艺理论做出了巨大的贡献,而且不遗余力地翻译、介绍、研究马克思主义基本理论和美学,并以其影响力强烈呼吁加强马列主义经典... 详细信息

中华人民共和国成立以后,朱光潜开始学习马列主义并以此指导其学术研究,他不但为建立中国的马克思主义美学、文艺理论做出了巨大的贡献,而且不遗余力地翻译、介绍、研究马克思主义基本理论和美学,并以其影响力强烈呼吁加强马列主义经典论著的翻译、研究。与此同时,他还翻译了与马列主义有关的大量的西方美学、文化的文献,为我们深入、系统地掌握马列主义美学和基本理论做出了不可替代的贡献。尤其应该指出的是,他针对中国马列主义基本论著的翻译、选本、研究的局限(特别是其薄弱、错误之处),有的放矢地纠正了许多错误,甚至重译一些经典论著,并提出了克服这些局限的有效的工作机制和具体做法。

关键词：朱光潜马列主义理论马列主义美学文论翻译