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Assessing and regulating the impacts of climate change on water resources in the Heihe watershed on the Loess Plateau of{2.

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Science china Earth Sciences 2010年第5期53卷 710-720页

作者： li zhi1,2. liu wenzhao2. zhang xunchang3 & zheng fenli2.1 college of resources and environment,{2., yangling 712.00, china 2.Institute of Soil and Water Conservation, Chinese Academy of Sciences & Ministry of Water resources, yangling 712.00, china 3 USDA-ARS Grazinglands Research Laboratory, 73036, El Reno, Oklahoma, USA 1. college of resources and {2. Northwest Agriculture & Forestry University Yangling 712100 China2. Institute of Soil and Water Conservation Chinese Academy of Sciences & Ministry of Water Resources Yangling 712100 China3. USDA-ARS Grazinglands Research Laboratory El Reno Oklahoma 73036 USA

Climate change can cause considerable changes in water resources and assessing the potential impacts can provide important information for regional sustainable development. The objectives were to evaluate the possible impacts of climate change during 2.10-2.39 on water resources (runoff, soil water content, and evapotranspiration) in the Heihe watershed on the Loess Plateau of china and to further explore adaptive measures to cope with the changes. Projections of four climate models (CCSR/NIES, CGCM2. CSIRO-Mk2. and HadCM3) under three emission scenarios (A2. B2. and GGa) were used to estimate future changes in precipitation, maximum and minimum temperature based on Change Factor method. The Soil and Water Assessment Tool (SWAT) was employed to simulate the hydrologic responses to climate changes. The CA-Markov model was used to develop land use scenarios. Compared with the present climate, the climate models predicted a -2.3% to 7.8% change in annual precipitation, 0.7 to 2.2.C rises in maximum temperature, and 1.2.to 2.8°C rises in minimum temperature. Without consideration of land use change, SWAT predicted a -19.8% to 37.0% change for runoff, -5.5% to 17.2. change for soil water content, and 0.1% to 5.9% increase for evapotranspiration during 2.10–2.39 under all climate scenarios. Though the change of hydrometeorolocial variables are complex, they would possibly increase with great probability, and the hydrological regime would be influenced such as a decrease in runoff in winter months. With land use changes, the projected land use of 2.15 would increase soil water content by 4.0% and surface runoff by 5.7% while slightly decrease evapotranspiration by 0.6% compared with the 2.00 land use. This result showed that adjustment of land use patterns was capable of regulating water resources and could be used to mitigate the adverse effects of climate change.

关键词： climate change water resources SWAT the Loess Plateau

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不同煤泥添加量对紫花苜蓿根际土壤酶活性与微生物的影响

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干旱区资源与环境 2024年第10期38卷 144-153页

作者：张少卓王征刘志昌杨志新李玉灵河北农业大学林学院保定071000 鄂尔多斯市乌兰煤炭(集团)有限责任公司鄂尔多斯017200

煤泥特殊的结构和组成使其在保水蓄肥和土壤生境构建方面有积极作用,这为煤泥在退化土地生态修复、农林牧业上的利用提供了重要的理论依据和广阔的应用前景。明确煤泥对植物、微生物及其土壤生物环境的作用利弊是煤泥生态利用的前提。为... 详细信息

煤泥特殊的结构和组成使其在保水蓄肥和土壤生境构建方面有积极作用,这为煤泥在退化土地生态修复、农林牧业上的利用提供了重要的理论依据和广阔的应用前景。明确煤泥对植物、微生物及其土壤生物环境的作用利弊是煤泥生态利用的前提。为此,为了探究煤泥生物环境特征,探索煤泥资源化利用的新方向,提高煤泥利用率,文中设计了煤泥与沙土0:4(CK)、2.2.CS-1)、3:1(CS-2.和4:0(CS-3)的4种混合比例,进行紫花苜蓿(Medicago sativa L.)盆栽种植试验,分析煤泥对苜蓿根际土壤酶活性和土壤微生物群落结构的影响。结果表明:1)土壤脲酶活性、过氧化氢酶活性随煤泥添加量的增加而降低。脱氢酶活性随煤泥添加量的增加而升高,CS-1土壤碱性磷酸酶活性最高。2.土壤细菌多样性指数随煤泥添加量增加而降低。CS-1土壤真菌多样性指数最高。3)变形菌门(Proteobacteria)均为各处理中细菌群落的优势菌门。子囊菌门(Asco⁃mycota)为各处理中真菌群落的优势菌门。NMDS分析表明煤泥对细菌群落结构影响较大。4)相关性分析发现,土壤脲酶活性、过氧化氢酶活性、脱氢酶活性与细菌多样性指标相关性显著(P0.05)。冗余分析表明多个土壤微生物优势种群与土壤酶活性显著相关(P1处理土壤微生物多样性和酶活性相对较高,为煤泥资源化利用提供了参考依据。

关键词：煤泥紫花苜蓿根际土壤土壤酶活性土壤微生物

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土坷垃与泥疙瘩:如何认识和表征土壤团聚体生物物理结构?

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地球科学进展 2024年第8期39卷 772-787页

作者：赵正冯潇刘成陈硕桐刘志伟王燕夏少攀刘晓雨卞荣军张旭辉程琨郑聚锋李恋卿潘根兴南京农业大学资源环境学院土壤学系江苏南京210095 南京农业大学农业资源与生态环境研究所江苏南京210095 太原工业学院环境与安全工程系山西太原030008 浙江科技大学环境与资源学院浙江杭州310008 扬州大学环境科学与工程学院江苏扬州225127

近2.年来土壤团聚体的研究逐渐成为土壤学研究的主要方向,其理论和方法不断推陈出新。通过回顾土壤团聚体形成发育的基本理论沿革,梳理了土壤团聚体粒径分组、组分分析和结构表征的技术沿革,讨论和归纳了土壤团聚体分离制备和生物物理... 详细信息

近2.年来土壤团聚体的研究逐渐成为土壤学研究的主要方向,其理论和方法不断推陈出新。通过回顾土壤团聚体形成发育的基本理论沿革,梳理了土壤团聚体粒径分组、组分分析和结构表征的技术沿革,讨论和归纳了土壤团聚体分离制备和生物物理结构解剖分析的技术方法,并凝练了对土壤团聚体系统的本质科学认识。得到的主要认识包括:(1)土壤团聚体是土壤中矿物质、有机质和生物质相互作用构成的最小微域构造单元,应赋予功能性土壤颗粒的本质地位;(2.土壤中不同层级团聚体的不同微域分布格式是土壤异质性和功能多样性的原由;(3)土壤团聚体的最终本质是土壤(生物)动态孔隙系统,不同层级团聚体在总孔度、孔径及其堆砌构造上或者说团聚体-孔隙的偶联构造上具有固有的特征;(4)主要可从宏团聚体-微团聚体-(未团聚的)粉黏粒3个层级的组合关系来表征土壤团聚体系统,可用“石榴”模式来理解粉黏粒和微团聚体进一步发育为宏团聚体;(5)在团聚体分离制备方法中,尽管干筛法和润筛法对于干土样本应用较多,但湿筛法制备的样品更接近于团聚体的自然形成和稳定;(6)计算机断层扫描孔隙微形貌技术提供了刻画团聚体孔隙系统并与土壤生命过程和生态系统服务相联系的定量化和可视化技术途径。未来需要通过广泛国际合作发展全球土壤团聚体系统的定量化解剖、功能化分析和可视化呈现的通用方法和实验室操作规范,以便从团聚体系统层面解构土壤复杂系统,特别是生物多样性系统,为开发基于团聚体理论的全球土壤管理政策及技术路径提供科学依据和参考。

关键词：土壤团聚体团聚体系统层级系统孔隙系统生物物理结构 CT微形貌学

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Emerging contaminants:A One Health perspective

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The Innovation 2024年第4期5卷 140-170,139页

作者： Fang Wang Leilei Xiang Kelvin Sze-Yin Leung Martin Elsner Ying zhang Yuming Guo Bo Pan Hongwen Sun Taicheng An Guangguo Ying Bryan WBrooks Deyi Hou Damian EHelbling Jianqiang Sun Hao Qiu Timothy MVogel Wei zhang Yanzheng Gao Myrna JSimpson Yi Luo Scott XChang Guanyong Su Bryan MWong Tzung-May Fu Dong Zhu Karl JJobst Chengjun Ge Frederic Coulon Jean Damascene Harindintwali Xiankui Zeng Haijun Wang Yuhao Fu Zhong Wei Rainer Lohmann Changer Chen Yang Song Concepcion Sanchez-Cid Yu Wang Ali El-Naggar Yiming Yao Yanran Huang Japhet Cheuk-Fung Law Chenggang Gu Huizhong Shen Yanpeng Gao Chao Qin Hao li Tong zhang Natàlia Corcoll Min liu Daniel SAlessi Hui li Kristian KBrandt Yolanda Pico Cheng Gu Jianhua Guo Jianqiang Su Philippe Corvini Mao Ye Teresa Rocha-Santos Huan He Yi Yang Meiping Tong Weina zhang Fidèle Suanon Ferdi Brahushi Zhenyu Wang Syed AHashsham Marko Virta Qingbin Yuan Gaofei Jiang Louis A.Tremblay Qingwei Bu Jichun Wu Willie Peijnenburg Edward Topp Xinde Cao Xin Jiang Minghui zheng Taolin zhang Yongming Luo lizhong Zhu Xiangdong li DamiàBarceló Jianmin Chen Baoshan Xing Wulf Amelung Zongwei Cai Ravi Naidu Qirong Shen Janusz Pawliszyn Yong-guan Zhu Andreas Schaeffer Matthias C.Rillig Fengchang Wu Gang Yu James M.Tiedje State Key Laboratory of Soil and Sustainable agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China university of Chinese Academy of Sciences Beijing 100049China Department of Chemistry Hong Kong Baptist UniversityHong KongChina HKBU Institute of Research and Continuing Education Shenzhen Virtual University ParkShenzhenChina Technical university of Munich TUM School of Natural SciencesInstitute of Hydrochemistry85748 GarchingGermany School of resources&environment Northeast Agricultural UniversityHarbin 150030China Climate Air Quality Research UnitSchool of Public Health and Preventive MedicineMonash UniversityMelbourneVICAustralia Faculty of environmental Science&Engineering Kunming University of Science&TechnologyKunming 650500China Ministry of Education Key Laboratory of Pollution Processes and environmental Criteria College of Environmental Science and EngineeringNankai UniversityTianjin 300350China Guangdong Key Laboratory of environmental Catalysis and Health Risk Control School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China Ministry of Education Key Laboratory of environmental Theoretical Chemistry South China Normal UniversityGuangzhouGuangdong 510006China Department of environmental Science Baylor UniversityWacoTXUSA Center for Reservoir and Aquatic Systems Research(CRASR) Baylor UniversityWacoTXUSA School of environment Tsinghua UniversityBeijing 100084China School of Civil and environmental Engineering Cornell UniversityIthacaNY 14853USA Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province Zhejiang University of TechnologyHangzhou 310014China School of environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Laboratoire d’Ecologie Microbienne Universite Claude Bernard Lyon 1UMR CNRS 5557UMR INRAE 1418VetAgro Sup69622 VilleurbanneFrance Department of Pla

environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary *** global efforts to mitigate legacy pollutants,the continuous introduction of new substances remains a major threat to both people and the *** response,global initiatives are focusing on risk assessment and regulation of emerging contaminants,as demonstrated by the ongoing efforts to establish the UN’s Intergovernmental Science-Policy Panel on Chemicals,Waste,and Pollution *** review identifies the sources and impacts of emerging contaminants on planetary health,emphasizing the importance of adopting a One Health *** for monitoring and addressing these pollutants are discussed,underscoring the need for robust and socially equitable environmental policies at both regional and international *** actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.

关键词： pollution planet contaminants

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Interactions between phosphorus availability and microbes in a wheat–maize double cropping system:A reduced fertilization scheme

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Journal of Integrative agriculture 2022年第3期21卷 840-854页

作者： YU Xiao-jing CHEN Qi SHI Wen-cong GAO zheng SUN Xiao DONG Jing-jing li Juan WANG Heng-tao GAO Jian-guo {2. zhi-guang zhang Min National Engineering Research Center for Efficient Utilization of Soil and Fertilizer {2./college of {2. and environment/college of life Sciences Shandong Agricultural UniversityTai’an 271018P.R.China Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China Shandong Zhongxin Agricultural Development Co. Ltd.Linshu 276700P.R.China

Mechanisms controlling phosphorus(P) availability and the roles of microorganisms in the efficient utilization of soil P in the wheat–maize double cropping system are poorly *** the present study,we conducted a pot experiment for four consecutive wheat–maize seasons(2.16–2.18) using calcareous soils with high(30.36 mg kg^(–1)) and low(9.78 mg kg^(–1)) initial Olsen-P content to evaluate the effects of conventional P fertilizer application to both wheat and maize(Pwm) along with a reduced P fertilizer application only to wheat(Pw).The microbial community structure along with soil P availability parameters and crop yield were *** results showed that the Pw treatment reduces the annual P input by 33.3% without affecting the total yield for at least two consecutive years as compared with the Pwm treatment in the high Olsen-P *** water-soluble P concentrations in the Pw treatment were similar to those in the Pwm treatment at the 12.leaf collar stage when maize requires the most ***,the soil P content significantly affected soil microbial communities,especially fungal ***,the relative abundances of Proteobacteria and alkaline phosphatase(ALP) activity of Pw were significantly higher(by 11.4 and 13.3%) than those of Pwm in soil with high *** microfloral contribution to yield was greater than that of soil P content in soil with high *** abundances of Bacillus and Rhizobium were enriched in the Pw treatment compared with the Pwm *** showed a significant positive correlation with acid phosphatase(ACP) activity,and Rhizobium displayed significant positive correlations with ACP and ALP in soil with high Olsen-P,which may enhance P *** findings suggested that the application of P fertilization only to wheat is practical in high P soils to ensure optimal production in the wheat and maize double cropping system and that the soil P availability and microbial community may collabo

关键词： wheat–maize rotation Olsen-P alkaline phosphatase phosphorus fertilization calcareous soils

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COP9 signalosome subunit PfCsnE regulates secondary metabolism and conidial formation in Pestalotiopsis fici

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Science china(life Sciences) 2017年第6期60卷 656-664页

作者： Yanjing zheng Xiuna Wang Xiaoling {2. Wei li Gang liu Shihua Wang Xiufeng Yan Huixi Zou Wen-Bing Yin Zhejiang Provincial(Wenzhou) Key Lab for Water environment and Marine Biological resources Protection College of Life and Environmental Science Wenzhou University State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences School of life Sciences Fujian Agriculture and Forestry University college of life Sciences University of Chinese Academy of Sciences

The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal development and secondary metabolism(SM) are mediated by the fifth subunit of CSN, called CsnE. Here we uncover a connection with CsnE and conidial formation as well as SM regulation in the plant endophytic fungus Pestalotiopsis fici. A homology search of the P. fici genome with CsnE, involved in sexual development and SM in Aspergillus nidulans, identified PfCsnE. Deletion of PfcsnE resulted in a mutant that stopped conidial production, but the conidia are recovered in a PfcsnE complemented strain. This indicates that PfCsnE is required for the formation of conidia. Secondary metabolite analysis demonstrated that the ΔPfcsnE strain produced more chloroisosulochrin, less ficiolide A production in comparison to wild type(WT). Transcriptome analysis of WT andΔPfcsnE strains indicated that PfcsnE impacts the expression levels of 8.37% of 14,797 annotated genes. Specifically, nine biosynthetic gene clusters(BGCs) were up-regulated and three BGCs were down-regulated by PfCsnE. Our results suggest that PfCsnE plays major roles in SM regulation and conidial development in P. fici.

关键词： COP9 signalosome complex CSN secondary metabolism conidial development endophytic fungi

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Functional assembly of root-associated microbial consortia improves nutrient efficiency and yield in soybean

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Journal of Integrative Plant Biology 2021年第6期63卷 1021-1035页

作者： Cunhu Wang Yanjun li Mingjia li Kefei {2. Wenjing Ma Lei zheng Hanyu Xu Baofeng Cui Ran liu Yongqing Yang Yongjia Zhong Hong liao Root Biology Center College of Resources and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002China

Root-associated microbes are critical for plant growth and nutrient acquisition. However, scant information exists on optimizing communities of beneficial root-associated microbes or the mechanisms underlying their interactions with host plants. In this report, we demonstrate that rootassociated microbes are critical influencers of host plant growth and nutrient acquisition. Three synthetic communities(SynComs) were constructed based on functional screening of 1,893 microbial strains isolated from root-associated compartments of soybean plants. Functional assemblage of SynComs promoted significant plant growth and nutrient acquisition under both N/P nutrient deficiency and sufficiency *** trials further revealed that application of SynComs stably and significantly promoted plant growth, facilitated N and P acquisition, and subsequently increased soybean yield. Among the tested communities, SynCom1 exhibited the greatest promotion effect, with yield increases of up to 36.1% observed in two field sites. Further RNA-seq implied that SynCom application systemically regulates N and P signaling networks at the transcriptional level, which leads to increased representation of important growth pathways, especially those related to auxin responses. Overall,this study details a promising strategy for constructing SynComs based on functional screening,which are capable of enhancing nutrient acquisition and crop yield through the activities of beneficial root-associated microbes.

关键词： growth promotion nitrogen phosphorus root-associated microbes SynCom soybean

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Modes of genetic adaptations underlying functional innovations in the rumen

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Science china(life Sciences) 2021年第1期64卷 1-21页

作者： Xiangyu Pan Yudong Cai Zongjun li Xianqing Chen Rasmus Heller Nini Wang Yu Wang Chen Zhao Yong Wang Han Xu Songhai li Ming li Cunyuan li Shengwei Hu Hui li Kun Wang Lei Chen Bin Wei Zhuqing zheng Weiwei Fu Yue Yang Tingting zhang Zhuoting Hou Yueyang Yan Xiaoyang Lv Wei Sun Xinyu li Shisheng Huang lixiang liu Shengyong Mao Wenqing liu Jinlian Hua {12.peng li Guojie zhang Yulin Chen Xihong Wang Qiang Qiu Brian PDalrymple Wen Wang Yu Jiang Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling712100China School of Ecology and environment Northwestern Polytechnical UniversityXi’an710072China Section for Computational and RNA Biology Department of BiologyUniversity of CopenhagenCopenhagenDK-2100Denmark university of Chinese Academy of Sciences Beijing100049China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming650223China CEMS NCMISMDISAcademy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing100080China Marine Mammal and Marine Bioacoustics Laboratory Institute of Deep-sea Science and EngineeringChinese Academy of SciencesSanya572000China college of life Sciences Shihezi UniversityShihezi832003China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Animal Science and TechnologyGuangxi UniversityNanning530005China college of Animal Science and Technology Yangzhou UniversityYangzhou225009China Joint International Research Laboratory of agriculture and Agri-Product Safety of Ministry of Education of china Yangzhou UniversityYangzhou225009China Key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou510150China School of life Science and Technology Shanghai Tech UniversityShanghai201210China college of Animal Science and Technology Nanjing Agricultural UniversityNanjing210095China college of Veterinary Medicine Shaanxi Centre of Stem Cells Engineering&TechnologyNorthwest A&F UniversityYangling712100China Department of Special Economic Animal Nutrition and Feed Science Institute of Special Animal and Plant SciencesChinese Academy of Agricultural SciencesChangchun130112China Section for Ecology and Evolution Department of BiologyUniversity of CopenhagenCopenhagenDK-2100Denmark china National GeneBank BGI-ShenzhenShen

The rumen is the hallmark organ of ruminants and hosts a diverse ecosystem of microorganisms that facilitates efficient digestion of plant *** analyzed 897 transcriptomes from three Cetartiodactyla lineages:ruminants,camels and cetaceans,as well as data from ruminant comparative genomics and functional assays to explore the genetic basis of rumen functional *** identified genes with relatively high expression in the rumen,of which many appeared to be recruited from other *** genes show functional enrichment in ketone body metabolism,regulation of microbial community,and epithelium absorption,which are the most prominent biological processes involved in rumen *** modes of genetic change underlying rumen functional innovations were uncovered,including coding mutations,genes newly evolved,and changes of regulatory *** validated that the key ketogenesis rate-limiting gene(HMGCS2.with five ruminant-specific mutations was under positive selection and exhibits higher synthesis activity than those of other *** newly evolved genes(LYZ1 and DEFB1)are resistant to Gram-positive bacteria and thereby may regulate microbial community ***,we confirmed that the changes of regulatory elements accounted for the majority of rumen gene *** results greatly improve our understanding of rumen evolution and organ evo-devo in general.

关键词： rumen innovations comparative transcriptomics comparative genomics evo-devo evolution of organs

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Additive effects of warming and grazing on fine-root decomposition and loss of nutrients in an alpine meadow

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Journal of Plant Ecology 2022年第6期15卷 1273-1284页

作者： Yang Zhou Wang-Wang Lv Shi-Ping Wang li-Rong zhang Jian-Ping Sun li-li Jiang Pei-Pei liu Qi Wang Bo-Wen li AWang Huan Hong Su-Ren zhang Lu Xia Nan Ji zheng-Xin Xie Cai-Yun Luo Zhen-Hua zhang Chang-Hui Wang Jin-zhi Wang Ci Yang Tsechoe Dorji Key Laboratory of Alpine Ecology Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China university of the Chinese Academy of Sciences Beijing 100049China CAS Center for Excellence in Tibetan Plateau Earth Science of the Chinese Academy of Sciences Beijing 100101China Department of resources and environment Hebei Normal University for NationalitiesChengde 067600China Science college Tibet UniversityLhasa 850001China northwestern Institute of Plateau Biology Chinese Academy of SciencesQinghai Provincial Key Laboratory of Restoration Ecology of Cold AreaXining 810008China Grassland college Shanxi Agriculture UniversityTaigu 030801China Wetland Institute Chinese Academy of ForestryBeijing 100093China Center for Meteorology Meteorological Bureau of Tibet Autonomous RegionLhasa 850000China

Fine-root decomposition is a critical process regulating ecosystem carbon cycles and affecting nutrient cycling and soil ***,whether interaction between warming and grazing affects fine-root decomposition is still under-researched in natural grasslands.A two-factorial experiment with asymmetric warming(*** *** and growing season *** season)and moderate grazing(*** average 50%forage utilization rate)was conducted to explore whether warming and grazing affect fine-root decomposition and loss of nutrients during a 2.year decomposition period in an alpine meadow on the Tibetan *** warming and grazing facilitated carbon cycling through increase in fine-root decomposition,and influenced element cycling which varies among *** effects of warming and grazing on fine-root decomposition and loss of nutrients were *** warming and grazing significantly increased cumulative percentage mass loss and total organic carbon loss of fine roots during the 2.year *** warming with grazing treatment reduced percentage nitrogen loss,whereas warming,regardless of grazing,decreased percentage phosphorus *** and grazing alone increased percentage loss of potassium,sodium,calcium and magnesium compared with *** were no interactions between warming and grazing on fine-root decomposition and loss of *** was greater temperature sensitivity of decreased phosphorus loss than that of decreased nitrogen *** temperature sensitivities of percentage loss of nutrients from fine-root decomposition would alter ratios of the available nutrients in soils,and may further affect ecosystem structure and functions in future warming.

关键词： root decomposition organic carbon loss loss of nutrients warming and grazing alpine meadow Tibetan Plateau

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焦化厂地肤根内解芘细菌的筛选及促生潜力

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应用生态学报 2019年第6期30卷 2056-2062页

作者：曹阳宋立超钮旭光张薇刘新政吴珊李思楠李海燕沈阳农业大学土地与环境学院/农业部东北耕地保育重点实验室/土肥资源高效利用国家工程实验室

从多环芳烃耐受植物根内分离具多环芳烃降解功能的内生细菌并研究其促生特性,为内生菌协同宿主植物修复多环芳烃污染土壤提供基础.以长期受多环芳烃污染的焦化厂区生长的地肤为材料,从其根内分离出以芘和1-氨基环丙烷-1-羧酸(ACC)为唯... 详细信息

从多环芳烃耐受植物根内分离具多环芳烃降解功能的内生细菌并研究其促生特性,为内生菌协同宿主植物修复多环芳烃污染土壤提供基础.以长期受多环芳烃污染的焦化厂区生长的地肤为材料,从其根内分离出以芘和1-氨基环丙烷-1-羧酸(ACC)为唯一碳源和氮源的内生细菌8株.通过芘降解试验,筛选得到3株高效芘降解内生细菌KSE4、KSE7和KSE8,经鉴定分别为芽孢杆菌属、假单胞菌属和鞘氨醇菌属.通过液体培养试验,研究了3株菌在芘胁迫下产ACC脱氨酶的能力和对地肤种子萌发的影响.结果表明:随着芘浓度(0~15 mg·L-1)的升高,ACC脱氨酶活性降低,其中KSE7的效果最好,在芘浓度为15 mg·L-1时,地肤发芽率和芽长分别比对照提高了44.8%和61.1%,在地肤-微生物修复焦化厂污染土壤的修复中具有一定的应用潜力.

关键词： ACC脱氨酶根内生细菌地肤多环芳烃降解

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