检索结果-南通市图书馆

Nuclear magnetic resonance based metabolomic differentiation of different Astragali Radix

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2017年第5期15卷 363-374页

作者： li Ai-Ping li zhen-yu QU Ting-li QIN Xue-Mei DU Guan-Hua Modern Research Center for Traditional Chinese Medicine Shanxi University Shanxi 030006 China College of Chemistry and Chemical Engineering Shanxi University Shanxi 030006 China School of Pharmaceutical Science Shanxi Medical University Shanxi 030006 China Institute of Materia Medica Chinese Academy of Medical Sciences Beijing 100050 China

Astragali Radix(AR) is one of the most popular herbal medicines in traditional Chinese medicine(TCM). Wild AR is believed to be of high quality, and substitution with cultivated AR is frequently encountered in the market. In the present study, two types of ARs(wild and cultivated) from Astragalus membranaceus(Fisch.) Bge. and A. membranaceus var. mongholicus(Bge.) Hsiao, growing in different regions of China, were analyzed by NMR profiling coupled with multivariate analysis. Results showed that both could be differentiated successfully and cultivation patterns or growing years might have greater impact on the metabolite compositions than the variety; the metabolites responsible for the separation were identified. In addition, three extraction methods were compared and the method(M1) was used for further analysis. In M1, the extraction solvent composed of water, methanol, and chloroform in the ratio of 1 : 1 : 2 was used to obtain the aqueous methanol(upper layer) and chloroform(lower layer) fractions, respectively, showing the best separation. The differential metabolites among different methods were also revealed. Moreover, the sucrose/glucose ratio could be used as a simple index to differentiate wild and cultivated AR. Meanwhile, the changes of correlation pattern among the differential metabolites of the two varieties were found. The work demonstrated that NMR-based non-targeted profiling approach, combined with multivariate statistical analysis, can be used as a powerful tool for differentiating AR of different cultivation types or growing years.

关键词： Nuclear Magnetic Resonance Chemical profiling Astragali Radix Cultivation patterns Variety Regions

Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella linnaeus

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2014年第11期13卷 2452-2459页

作者： HU zhen-di FENG Xia liN Qing-sheng CHEN Huan-yu li zhen-yu YIN Fei liANG Pei GAO Xi-wu Institute of Plant Protection Guangdong Academy of Agricultural Sciences Guangdong Provincial Key Laboratory of High Technology for Plant Protection Department of Entomology China Agricultural University

The insecticide chlorantraniliprole exhibits good efifcacy and plays an important role in controlling the diamondback moth, Plutella xylostella linnaeus. However, resistance to chlorantraniliprole has been observed recently in some ifeld populations. At present study, diamondback moths with resistance to chlorantraniliprole （resistant ratio （RR） was 82.18） for biochemical assays were selected. The assays were performed to determine potential resistance mechanisms. The results showed that the selected resistant moths （GDLZ-R） and susceptible moth could be synergized by known metabolic inhibitors such as piperonyl butoxide （PBO）, triphenyl phosphate （TPP） and diethyl-maleate （DEM） at different levels （1.68-5.50-fold and 2.20-2.89-fold, respectively）, and DEM showed the maximum synergism in both strains. In enzymes assays, a high level of glutathione-S-transferase （GST） was observed in the resistant moth, in contrast, moths that are susceptible to the insecticide had only 1/3 the GST activity of the resistant moths. The analysis of short-term exposure of chlorantraniliprole on biochemical response in the resistant strain also showed that GST activity was signiifcantly elevated after exposure to a sub-lethal concentration of chlorantraniliprole （about 1/3 LC50, 12 mg L-1） 12 and 24 h, respectively. The results show that there is a strong correlation between the enzyme activity and resistance, and GST is likely the main detoxiifcation mechanism responsible for resistance to chlorantraniliprole in P. xylostella L., cytochrome P450 monooxygenase （P450） and carboxy-lesterase （CarE） are involved in to some extent.

关键词： Plutella xylostella chlorantraniliprole resistance biochemical mechanism

cDNA cloning and characterization of t he carboxylesterase pxCCE016b from the diamondback moth, Plutella xylostella L.

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2016年第5期15卷 1059-1068页

作者： HU zhen-di FENG Xia liN Qing-sheng CHEN Huan-yu li zhen-yu YIN Fei liANG Pei GAO Xi-wu Department of Entomology China Agricultural University Beijing 100193 P.R.China Institute of Plant Protection Guangdong Provincial Key Laboratory of High Technology for Plant Protection Guangdong Academy of Agricultural Sciences Guangzhou 510640 P.R. China

Carboxylesterase is a multifunctional superfamily and can be found in almost all living organisms. As the metabolic enzymes, carboxylesterases are involved in insecticides resistance in insects for long time. In our previous studies, the enhanced c arboxylesterase activities were found in the chlorantraniliprole resistance strain of diamondback moth（DBM）. However, t he related enzyme gene of chlorantraniliprole resistance has not been clear in this strain. Here, a full-length c DNA of carboxylesterase pxCCE016 b was cloned and exogenously expressed in Escherichia coli at the first time, which contained a 1 693 bp open reading frame（ORF） and encoded a protein of 542 amino acids. Sequence analysis showed that this c DNA has a predicted mass of 61.56 k Da and a theoretical isoelectric point value of 5.78. The sequence of deduced amino acid possessed the classical structural features： a type-B carboxylesterase signature 2（EDCLYLNVYTK）, a type-B carboxylesterase serine active site（FGGDPENITIFGESAG） and the catalytic triad（S er186, Glu316, and His444）. The real-time quantitative PCR（q PCR） analysis showed that t he expression level of the p x CCE016 b was significantly higher in the chlorantraniliprole resistant strain than in the susceptible strain. Furthermore, pxCCE016 b was highly expressed in the midgut and epidermis of the DBM larvae. When the 3rd-instar larvae of resistant DBM were exposed to abamectin, alpha-cypermethrin, chlorantraniliprole, spinosad, c hlorfenapyr and indoxacarb insecticides, the up-regulated expression of pxCCE016 b was observed only in the group treated by chlorantraniliprole. In addition, recombinant vector p ET-pxCCE016 b was constructed with the most coding region（1 293 bp） and large number of soluble recombinant proteins（less than 48 k Da） were expressed successfully with prokaryotic cell. Western blot analysis showed that it was coded by pxCCE016 b. All the above findings provide important information for further f unctional study, althou

关键词： Plutella xylostella carboxylesterase chlorantraniliprole insecticide resistance pxCCE016b

Metabolomics coupled with similarity analysis advances the elucidation of the cold/hot properties of traditional Chinese medicines

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2017年第8期15卷 631-640页

作者： JIA Yan ZHANG zheng-zheng WEI yu-Hai Qin Xue-Mei li zhen-yu Modern Research Center for Traditional Chinese Medicine of Shanxi University Taiyuan 030006China College of Chemistry and Chemical Engineering of Shanxi University Taiyuan 030006China Qinghai Entry-Exit Inspection and Quarantine Bureau Xining 810000China

It recently becomes an important and urgent mission for modern scientific research to identify and explain the theory of traditional Chinese medicine(TCM), which has been utilized in China for more than four millennia. Since few works have been contributed to understanding the TCM theory, the mechanism of actions of drugs with cold/hot properties remains unclear. In the present study, six kinds of typical herbs with cold or hot properties were orally administered into mice, and serum and liver samples were analyzed using an untargeted nuclear magnetic resonance(NMR) based metabolomics approach coupled with similarity analysis. This approach was performed to identify and quantify changes in metabolic pathways to elucidate drug actions on the treated mice. Our results showed that those drugs with same property exerted similar effects on the metabolic alterations in mouse serum and liver samples, while drugs with different property showed different effects. The effects of herbal medicines with cold/hot properties were exerted by regulating the pathways linked to glycometabolism, lipid metabolism, amino acids metabolism and other metabolic pathways. The results elucidated the differences and similarities of drugs with cold/hot properties, providing useful information on the explanation of medicinal properties of these TCMs.

关键词： Cold/hot property Metabolomics Traditional Chinese medicine NMR Similarity analysis

Pharmacokinetics and tissue distribution of intraperitoneal 5-fluorouracil with a novel carrier solution in rats

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第14期14卷 2179-2186页

作者： Zhi-Gang Wei Guo-Xin li Xiang-Cheng Huang li zhen Jiang yu Hai-Jun Deng Shan-Hua Qing Ce Zhang Department of General Surgery Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China

AIM: To compare the pharmacokinetics and tissue distribution of 5-fluorouracil administered intraperitoneally with two isotonic carrier solutions: HAES-steri (neotype 6% hydroxyethyl starch), a novel carrier solution with middle molecular weight and physiologic saline (0.9% sodium chloride solution), a traditional carrier solution for intraperitoneal chemotherapy, in rats. METHODS: A total of 60 Sprague Dawley rats were randomized into groups according to the carrier solution administered. Each group was further randomized according to the intraperitoneal dwell period (1, 3, 6, 12, 18 and 24 h). At the end of the procedure the rats were killed, the peritoneal fluid was withdrawn completely and quantitated. Drug concentrations in peritoneal fluid, plasma, and tissues were determined by high- performance liquid chromatography. RESULTS: The mean volumes remaining in the peritoneal cavity were significantly higher with HAES- steri than those with physiologic saline at 1, 6, 12, 18, and 24 h (P = 0.047, 0.009, 0.005, 0.005 and 0.005 respectively, the percentages of remaining peritoneal fluid volume were 89.9 ± 5.6 vs 83.4 ± 4.9, 79.9 ± 2.8 vs 56.2 ± 15.7, 46.8 ± 5.5 vs 24.7 ± 9.7, 23.0 ± 2.8 vs 0.0 ± 0.0 and 4.2 ± 1.7 vs 0.0 ± 0.0 respectively). Mean concentrations in peritoneal fluid were significantly higher with HAES-steri than those with physiologic saline at 3, 12 and 18 h (P = 0.009, 0.009 and 0.005 respectively, the concentrations were 139.2768 ± 28.2317 mg/L vs mg/L, 11.5427 ± 3.0976 mg/L vs 0.0000 ± 0.0000 mg/L and 4.7724 ± 1.0936 mg/L vs 0.0000 ± 0.0000 mg/L respectively). Mean plasma 5-fluorouracil concentrations in portal vein were significantly higher with HAES-steri at 3, 12, 18 and 24 h (P = 0.009, 0.034, 0.005 and 0.019 respectively, the concentrations were 3.3572 ± 0.8128 mg/L vs 0.8794 ± 0.2394 mg/L, 0.6203 ± 0.9935 mg/L vs 0.0112 ± 0.0250 mg/L, 0.3725 ± 0.3871 mg/L vs 0.0000 ± 0.0000 mg/L, and 0.2469 ± 0.1457 mg/L vs 0.0000 ± 0.0000 mg/L respectively),

关键词： Carrier solutions Intraperitoneal chemo- therapy 5-fluorouracil Pharmacokinetics Tissue distribution

Decoration of Electrospun Nanofibers with Magnetic Nanoparticles via Electrospinning and Sol-gel Process

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2010年第5期26卷 847-850页

作者： YANG Fan zhenG Wei HUANG Hui-min li zhen-yu ZHANG Hong-nan WANG Wei WANG Ce Alan G. MacDiarmid Institute Jilin University Changchun 130012 P. R. China

Polyacrylonitrile（PAN）/Fe3O4 composite nanofibers were successfully obtained through electrospinning and sol-gel technology. The resulting magnetic Fe3O4 nanoparticles were homogeneously distributed on the surface of PAN nanofibers and the diameters of polyacrylonitrile nanofibers and nanoparticles were easily controlled, respectively. The distribution of Fe3O4 nanoparticles inside the nanofibrous composite was investigated by field emission scanning electron microscopy and transmission electron microscopy. X-ray diffraction reveals the presence of Fe3O4 nanoparticles in the composite nanofiber. The resulting sample shows a super paramagnetic behavior.

关键词： Electrospinning Magnetic Fe3O4 Nanoparticle Sol-gel

Clinical Observation on Three-character-scripture School Pediatric Tuina for Infants with Night Crying:A Report of 60 Cases

在线全文

学校读者我要写书评

暂无评论

Journal of Acupuncture and Tuina Science 2013年第1期11卷 22-25页

作者： Bi Jian-qing li yu-zhen Dai Ni-ni Ni Wen-ting Qingdao Haici Medical Group

Objective: To observe the clinical efficacy of Three-character-scripture School pediatric tuina for infants with night crying. Methods: Sixty cases with night crying were treated with Three-character- scripture School pediatric tuina, followed by observing the efficacy. Results: Forty-nine cases were recovered, 7 cases improved, and 4 cases failed. The total effective rate was 93.3%. Conclusion: Three-character-scripture School pediatric tuina is effective for night crying of infants. It should be spread and applied due to its manipulation with less acupoints, simple operation and no pain for infants.

关键词： Tuina, Massage Night Crying Infant

Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第2期17卷 548-565页

作者： Ming‑yuan yu Jing Wu Guang Yin Fan‑zhen Jiao Zhong‑zhen yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

在线全文

学校读者我要写书评

暂无评论

BMF促细胞凋亡研究进展

生物化学与生物物理进展 2017年第9期44卷 751-756页

作者：李玉珍解放军总医院医学保障部基础医学所病理生理研究室北京100853

BMF(Bcl-2-modifying factor)是一种具有促凋亡作用的Bcl-2家族成员.在正常生理状态下,内源性的BMF持续锚定在胞浆中的细胞骨架上,时刻感受细胞内的变化.一旦损伤刺激作用于细胞,BMF从胞浆转移至线粒体,触发线粒体凋亡途径,造成细胞损伤... 详细信息

BMF(Bcl-2-modifying factor)是一种具有促凋亡作用的Bcl-2家族成员.在正常生理状态下,内源性的BMF持续锚定在胞浆中的细胞骨架上,时刻感受细胞内的变化.一旦损伤刺激作用于细胞,BMF从胞浆转移至线粒体,触发线粒体凋亡途径,造成细胞损伤.BMF的促凋亡作用受到转录、翻译和翻译后修饰的调控,过表达或者转录上调的BMF主要定位于线粒体,诱导细胞凋亡.由此可见,BMF具有强大的促凋亡功能.

关键词： BMF 促凋亡蛋白细胞凋亡