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基于Spring Boot+Vue框架的羊场综合管理系统软件的设计与应用

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家畜生态学报 2024年第3期45卷 55-62页

作者：姜韶华常星海高云帆南风马东李书琴陈玉林张恩平西北农林科技大学动物科技学院陕西杨凌712100 西北农林科技大学信息工程学院陕西杨凌712100 陕西省榆林市羊产业发展中心陕西榆林719000

本研究旨在辅助羊场生产数据规范化管理及良种繁育智能科学化应用,采用MySQL数据库及Java、R编程语言,基于Spring Boot+Vue框架设计了一套羊场综合管理系统软件。该系统优化了羊只信息管理、羊场配种方案,实现生产数据可视化,设置预警... 详细信息

本研究旨在辅助羊场生产数据规范化管理及良种繁育智能科学化应用,采用MySQL数据库及Java、R编程语言,基于Spring Boot+Vue框架设计了一套羊场综合管理系统软件。该系统优化了羊只信息管理、羊场配种方案,实现生产数据可视化,设置预警功能帮助羊场及时掌握羊只产羔时间与生产性能情况;系统设计羊只基本信息管理、生产信息、疫病信息、繁殖信息、药品管理、饲料管理、统计报表与预警、科学育种八个模块。其中,在科学育种管理子模块中,根据羊场育种需求,构建了三种育种值计算模型(BLUP模型、GLMM模型、平均表现型模型),结合种公母羊实际表现,实现种公母羊的等级排序并自动生成近交系数,得到科学合理的配种方案。本系统在陕北某集约化肉羊场、横山区某绒山羊养殖联合育种户、富平县某奶山羊养殖场进行为期半年的实际生产应用,通过跟踪核心群体,完善与优化系统各项功能,为羊场智慧化、数字化育种管理提供了数据平台,同时也为实现智慧羊场的发展增添了新版本1.0。

关键词： Spring Boot+Vue框架羊场信息管理系统育种

HLA-B-associated transcript 3 (Bat3) stabilizes and activates p53 in a HAUSP-dependent manner

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Journal of Molecular Cell Biology 2020年第2期12卷 99-112页

作者： Rui Zhang Di Cui TengXue Yue Lang yunfan Zhang lianjie li Haili Sun Yu Kuang Gebin li Jun Tang Department of Basic Veterinary College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China State Key Laboratory of Agrobiotechnology China Agricultural UniversityBeijing 100193China Department of Clinical Veterinary College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Clinical Research Institute Zhejiang Provincial People's HospitalPeople's Hospital of Hangzhou Medical CollegeHangzhou 310014China

The p53 pathway is a highly complex signaling network including several key *** is a critical component of the p53 pathway acting as a deubiquitinase for both p53 and its key repressor ***,we identified a novel HAUSP-interacting protein,HLA-B-associated transcript 3(Bat3)and found it to be capable of inducing p53 stabilization and activation via a HAUSP-dependent mechanism,resulting in cell growth ***,the deubiquitylating enzymatic activity of HAUSP was not required for this ***-immunoprecipitation showed that p53 coexisted in a complex with Bat3 and HAUSP in vivo,and HAUSP may serve as a binding mediator to enhance the interaction between p53 and *** studies revealed that formation of this three-protein complex interfered with the binding of p53 to its proteasome receptor S5a and promoted the accumulation of p53 in ***,Mdm2 protein abundance is also regulated by Bat3 in the presence of *** of Bat3 and HAUSP increases Mdm2 protein levels without influencing the p53–Mdm2 interaction and Mdm2-mediated p53 ubiquitination,indicating that Bat3–HAUSP-mediated protein stabilization is not specific to p53 and different mechanisms may be involved in Bat3-mediated regulation of p53–Mdm2 ***,our study unravels a novel mechanism by which p53 is stabilized and activated by HAUSP-mediated interaction with Bat3 and implies that Bat3 might function as a tumor suppressor through the stabilization of p53.

关键词： Bat3 p53 stabilization HAUSP S5a proteasome recognition

Cysteine mutations impair the structural stability of phosphomannomutase 2(PMM2)in glycosylation-associated metabolic disorders

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Genes & Diseases 2023年第5期10卷 1743-1746页

作者： yunfan Pan li lin Fan Yu Jingmiao Sun Guannan Bai Xinyu He Jianhua Mao lidan Hu The Children’s Hospital Zhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhouZhejiang 310052China Beijing Advanced Innovation Center for Structural Biology Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Department of Nephrology The Children's HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthNational Children's Regional Medical CenterHangzhouZhejiang 310052China

Glycosylation is a ubiquitous molecular modification in the process of cellular metabolism regulation,which plays a vital role in maintaining and regulating cellular *** in glycosylation enzymes may jeopardize the glycosylation process and eventually lead to the metabolic disease,manifesting with either a neurologic or multisystem phenotype,which is also known as congenital disorder of glycosylation(CDG).1 It could be classified into type I CDG and type II CDG,and the latter was referred to as the abnormal formation of nascent *** 2 CDG(PMM2-CDG),the most prevalent CDG with an incidence rate of 1 in 20,000 individuals,is mainly due to the metabolic disorder of phosphomannomutase 2(PMM2).2 PMM2 participates in the conversion of mannose-6-phosphate to *** cysteine residues,as one of the least abundant amino acids,have unique attributes to maintain the stability of protein structure,especially for catalytic activity and protein folding.3 Therefore,cysteine mutations have been identified in various diseases including *** by the crystal structure of PMM2(PDB ID:7O5Z),4 four cysteine mutations in the hPMM2,C9Y,C103F,C192G,and C241S,have been found in *** bioinformatic analysis by PROVEAN,the four cysteine mutations were predicted with“Deleterious”,ranking with the best two were C103F and C192G(C9Y:−5.493,C103F:−6.339,C192G:−11.176,C241S:−2.986).However,the underlying pathological mechanism is still *** them,C103F and C192G have only been reported in clinical study without a detailed explanation,so they were selected for our *** mutants were located near the linker region 1 and 2;C103F was located on the No.4αhelix and C192G was located on the No.7αhelix(Fig.S1A).The purpose of this study was to explore the structure and function roles of PMM2 clinical mutation and investigate the role of cysteine in PMM2 which might provide new insights into preventive and therape

关键词： stability eventually latter

遇CO_(2)自修复防腐固井水泥浆体系研究及应用

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石油化工应用 2024年第4期43卷 67-71页

作者：岳家平彭云帆武广瑷武治强李准中海油研究总院有限责任公司北京100028 中海油田服务股份有限公司河北燕郊065201

针对在CCUS井下难以维持水泥石长期完整性的问题,构建了一种遇CO_(2)自修复防腐固井水泥浆体系。该体系引入了自修复材料以及防腐材料,配合其他外加剂形成了密度1.9g/cm^(3)的水泥浆体系。该自修复水泥浆体系常规性能良好,能够达到固井... 详细信息

针对在CCUS井下难以维持水泥石长期完整性的问题,构建了一种遇CO_(2)自修复防腐固井水泥浆体系。该体系引入了自修复材料以及防腐材料,配合其他外加剂形成了密度1.9g/cm^(3)的水泥浆体系。该自修复水泥浆体系常规性能良好,能够达到固井施工的要求。研究了该体系的防腐性能以及自愈合性能,研究结果表明,腐蚀30d后水泥石的腐蚀深度小于1.00mm,CO_(2)腐蚀后抗压强度增强。腐蚀30d后,造缝自修复水泥石的抗压强度相对增长百分比为149.30%。腐蚀12h和24h后,水泥石的自修复率分别为97.64%和97.97%。本文构建的自修复防腐固井水泥浆体系能够有效推进CCUS技术发展以及助力CO_(2)上百年的封存。

关键词：固井水泥浆 CO_(2)自修复防腐 CCUS

The tumor suppressor CYLD controls epithelial morphogenesis and homeostasis by regulating mitotic spindle behavior and adherens junction assembly

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Journal of Genetics and Genomics 2017年第7期44卷 343-353页

作者： Wei Xie yunfan Yang Siqi Gao Ting Song Yuhan Wu Dengwen li Min liu Jun Zhou Key Laboratory ofAnimal Resistance Biology ofShandong Province Institute of Biomedical SciencesCollege ofLife Sciences.Shandong Normal University Jinun 250014China State Key Laboratory ofMedicinal Chemical Biology College of life SciencesNankai UniversityTianjin 300071China

Epithelial morphogenesis and homeostasis are essential for animal development and tissue regeneration, and epithelial disorganization is associated with developmental disorders and tumorigenesis. However, the molecular mechanisms that contribute to the morphogenesis and homeostasis of the epithelium remain elusive. Herein, we report a novel role for the cylindromatosis （CYLD） tumor suppressor in these events. Our results show that CYLD depletion disrupts epithelial organization in both Drosophila egg chambers and mouse skin and intestinal epithelia. Microscopic analysis of proliferating cells in mouse epithelial tissues and cultured organoids reveals that loss of CYLD synergizes with tumor-promoting agents to cause the misorientation of the mitotic spindle. Mechanistic studies show that CYLD accu- mulates at the cell cortex in epithelial tissues and cultured ceils, where it promotes the formation of epithelial adherens junctions through the modulation of microtuhule dynamics. These data suggest that CYLD controls epithelial morphogenesis and homeostasis by modulating the assembly of adherens junctions and ensuring proper orientation of the mitotic spindle. Our findings thus provide novel insight into the role of CYLD in development, tissue homeostasis, and tumorigenesis.

关键词： Epithelium CYLD Mitotic spindle Cell cortex Adherens junction

Neuronal-like differentiation of single versus multiple treatments with human amnion-derived mesenchymal stem cells induced by basic fibroblast growth factor

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Neural Regeneration Research 2009年第9期4卷 694-698页

作者： Hongliang Jiao Fangxia Guan Xiang Hu Jianbin li Hong Shan Wei li Jun li Ying Du Bo Yang yunfan Zhou Department of Neurosurgery First Hospital of Zhengzhou University Zhengzhou 450052 Henan Province China Department of Bioengineering Zhengzhou University Zhengzhou 450001 Henan Province China Shenzhen Beike Cell Engineering Institute Shenzhen 518057 Guangdong Province China Henan Province Red Cross Blood Center Zhengzhou 450012 Henan Province China Jiangsu Public Technology Service Platform of Stem Cells and Biotherapy Taizhou 225300 Jiangsu Province China Department of Microbiology and Immunology Medical School of Zhengzhou University Zhengzhou 450001 Henan Province China

BACKGROUND： Cultures from multiple portions of umbilical cord blood mesenchymal stem cells have been shown to undergo more rapid proliferation and attachment than single portions. OBJECTIVE： To observe growth of basic fibroblast growth factor （bFGF）-induced cultures of human amnion-derived mesenchymal stem cells （AMSCs） and differentiation into neuronal-like cells. DESIGN, TIME AND SETTING： Comparative observation. The study was performed at the Laboratory of Microbiology and Immunology, Basic Medical School of Zhengzhou University from January to May 2008. METHODS： Amnia from full-term, uterine-incision delivery were donated by 12 healthy women. AMSCs were obtained by cell separation and culture techniques, and were passaged and induced by bFGF. From the third passage, a total of 1 mLAMSCs, at a density of 1.0 × 10^4/mL, was separately harvested from six samples, which served as group A. A total of 1 mL AMSCs, at a density of 1.0 × 10^4/mL, was harvested separately from the remaining six samples, which served a group B. A total of 0.5 mL from the six samples of group A and 0.5 mL from the six samples of grot, B were combined to form group C. MAIN OUTCOME MEASURES： Differences in cell quantity among the three groups were compare by cell quantification and 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） analysis. Expression of a glial cell marker, neuron-specific enolase, and nestin was detected in the three groups by immunocytochemistry. RESULTS： Cell quantification and MTT analysis of live cells, as well as AMSC absorbance, were significantly greater in group C compared with groups A and B at 18 days of culture （P 〈 0.05）, anc no significant difference was observed between groups A and B. Glial fibrillary acidic protein, neuron-specific enolase, and nestin were expressed in all groups following bFGF induction. CONCLUSION： Mixed AMSC cultures promoted proliferation, and bFGF-induced AMSCs differentiated into neuronal-like cells.

关键词： amnion mesenchymal stem cells induction mixed culture

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船用核安全级电缆研制

电线电缆 2024年第1期67卷 21-25页

作者：王云帆樊群杨佳昱于帅李永江武汉第二船舶设计研究所武汉430064 南京全信传输科技股份有限公司南京211113

通过分析特殊功能和环境适应性需求下船用核安全级电缆的分类和性能要求,进行了船用核安全级控制和仪表通信电缆的结构设计、材料选择和试制开发,后对试制电缆产品进行全性能测试。结果表明,该产品达到设计要求。

关键词：船用电缆核安全关键性能测试

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蠕形螨睑缘炎中西医诊治进展

中医眼耳鼻喉杂志 2020年第4期10卷 226-229页

作者：李运帆李翔杜薇万婧雯易文华韩明江吴美玲高瑞雪何茜成都中医药大学四川成都610075 成都中医药大学附属医院四川成都610072

睑缘炎是睑缘表面、睫毛毛囊及其腺体组织的亚急性或慢性炎症。其病因复杂,一般与细菌感染、理化刺激、屈光不正、慢性结膜炎、溢泪、隐斜、不良卫生习惯和机体抵抗力下降有关,为临床常见眼病。根据临床不同特点,可分为三类:鳞屑性睑缘... 详细信息

睑缘炎是睑缘表面、睫毛毛囊及其腺体组织的亚急性或慢性炎症。其病因复杂,一般与细菌感染、理化刺激、屈光不正、慢性结膜炎、溢泪、隐斜、不良卫生习惯和机体抵抗力下降有关,为临床常见眼病。根据临床不同特点,可分为三类:鳞屑性睑缘炎、溃疡性睑缘炎、眦部睑缘炎。轻者导致患者眼部刺激症状,严重者可累及睑板腺和角膜而影响视力。近年来越来越多研究发现蠕形螨感染可能是睑缘炎的重要病因之一,但该病的诊治仍存在局限性。本文综述近年来蠕形螨睑缘炎中西医诊治进展,以供参考。

关键词：蠕形螨睑缘炎中西医诊治

硫化—胺法浮选菱锌矿体系中两相和三相泡沫的性能研究

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矿产保护与利用 2024年第3期44卷 74-80页

作者：李超罗溪梅齐琳萍宋振国王云帆昆明理工大学国土资源工程学院昆明理工大学复杂有色金属资源清洁利用国家重点实验室矿物加工科学与技术国家重点实验室昆明理工大学冶金与能源工程学院

硫化—胺法是氧化锌的主要选别方法之一，在工业生产中的指标较好，但存在浮选泡沫发黏、难消泡的问题。研究了硫化-胺法浮选菱锌矿体系中两相泡沫和三相泡沫的性能，考察了硫化钠用量、十二胺用量、矿物含量、矿物粒度、矿物种类等对... 详细信息

硫化—胺法是氧化锌的主要选别方法之一，在工业生产中的指标较好，但存在浮选泡沫发黏、难消泡的问题。研究了硫化-胺法浮选菱锌矿体系中两相泡沫和三相泡沫的性能，考察了硫化钠用量、十二胺用量、矿物含量、矿物粒度、矿物种类等对泡沫稳定性的影响，并测试了不同矿物含量的矿浆黏度。结果表明，在采用硫化—胺法浮选菱锌矿时，加入硫化钠能够提高两相和三相泡沫的稳定性。不同粒度的矿物颗粒对泡沫稳定性影响不同，加入-74+37μm粒级的菱锌矿和石英颗粒均能够降低泡沫稳定性，而加入-37μm粒级的菱锌矿和石英颗粒均能够提高泡沫稳定性，-18μm的石英颗粒影响尤为明显。当十二胺与微细粒级石英共同存在时，浮选泡沫尤为稳定。加入石英颗粒后的三相泡沫半衰期均高于菱锌矿，这是由于石英矿浆的黏度高于菱锌矿，因而泡沫液膜的Marangoni效应增强，导致排液速率变慢，泡沫液膜变薄速率下降，泡沫稳定性更强。

关键词：硫化—胺浮选法十二胺菱锌矿硫化钠石英泡沫半衰期