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Identification and genetic characterization of bovine hepacivirus in china:A large scale epidemiological study

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Virologica Sinica 2022年第2期37卷 223-228页

作者： Gang Lu Chaoxi Chen Ran Shao Juan Zhang Jinghao li Siqi Cai lintao Zhong Zhiying Lai Jiajun Ou Xin Yin Guihong Zhang Shoujun li college of Veterinary Medicine South China Agricultural UniversityGuangzhou510642China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteThe Chinese Academy of Agricultural SciencesHarbin150000China Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases Guangzhou510642China Guangdong Technological Engineering Research Center for Pet Guangzhou510642China college of life science and Technology Southwest Minzu UniversityChengdu610041China General Station of Forest and Grassland Pest Management National Forestry and Grassland AdministrationShenyang110000China

Bovine hepacivirus(BovhepV)is a novel virus that was recently discovered in Ghana and Germany in *** now,this virus has been identified in cattle population worldwide and is classified into subtypes *** fully understand the epidemic situation and genetic characteristic of BovhepV in china,a total of 612 cattle serum samples were collected from 20 farms in seven provinces and municipality in china between 2018 and 2020 and were tested for the presence of BovhepV RNA via semi-nested *** results demonstrated that 49(8.0%)samples were BovhepV *** is noted that BovhepV infection in yak was confirmed for the first *** was detected in all the seven provinces,with the positive rate ranging from 3.1%to 13.3%,which indicates a wide geographical distribution pattern of BovhepV in *** results revealed that 5'UTR of the 49 field BovhepV strains have a nucleotide similarity of 96.3%-100%between each other and 93.9%-100%with previously reported BovhepV *** addition,genetic analysis identified five critical nucleotide sites in 5'UTR to distinguish different subtypes,which was further verified by genomic sequencing and nucleotide similarity *** the 49 Chinese field BovhepV strains were classified into subtype G and this subtype is only determined in cattle in china *** study will provide insights for us to better understand the epidemiology and genetic diversity of BovhepV.

关键词： Bovine hepacivirus(BovhepV) china Epidemiology Genetic diversity Cattle

Holocene aeolian activities linked to Indian summer monsoon in the middle reaches of the Yarlung Zangbo River

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Journal of geographical sciences 2020年第12期30卷 2002-2014页

作者： li Tuoyu ZHANG Jifeng WU Yongqiu DU Shisong MO Duowen liAO Yinan CheN Zhitong liU Jianbao li Qing Capital normal university Beijing 100048China Key Laboratory of Alpine Ecology(LAE) Institute of Tibetan Plateau ResearchCASBeijing 100101China MOE Engineering Center of Desertification and Blown-sand Control Beijing Normal UniversityBeijing 100875China Laboratory for Earth Surface Processes Ministry of EducationCollege of Urban and Environmental SciencesPeking UniversityBeijing 100871China Institute of geographical sciences Hebei Engineering Research Center of Geographic Information ApplicationHebei Academy of SciencesShijiazhuang 050011China

Widespread aeolian deposits on the Tibetan Plateau(TP)have provided valuable palaeoclimatic ***,the primary factors(e.g.,climate factors,human activity,and vegetation cover)controlling aeolian deposition remain *** this paper,we use a dataset that comprises new and published ages of Holocene aeolian sand and loess in the middle reaches of the Yarlung Zangbo River to identify the primary controlling factors and palaeoclimatic implications of aeolian *** intervals of enhanced aeolian accumulation centered at 8.5-7.8,6.4-5.8,4.5-4.0,3.1-1.8,and 0.9 ka are identified,generally consistent with regional low rainfall events and weak Indian summer monsoon(ISM).This suggests that regional wetness,dominated by the ISM,may play a key role in modulating dust emissions and aeolian deposition on centennial *** results show that on centennial-to millennial-scales,ISM activity can be reconstructed by non-continuous aeolian deposits in the monsoon dominated TP.

关键词： Holocene aeolian activity dust Indian summer monsoon Tibetan Plateau

Mapping essential urban land use categories in china(EULUC-china):preliminary results for 2018

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science Bulletin 2020年第3期65卷 182-187页

作者： Peng Gong Bin Chen Xuecao li Han liu Jie Wang Yuqi Bai Jingming Chen Xi Chen Lei Fang Shuailong Feng Yongjiu Feng Yali Gong Hao Gu Huabing Huang Xiaochun Huang Hongzan Jiao Yingdong Kang Guangbin Lei Ainong li Xiaoting li Xun li yuechen li Zhilin li Zhongde li Chong liu Chunxia liu Maochou liu Shuguang liu Wanliu Mao Changhong Miao Hao Ni Qisheng Pan Shuhua Qi Zhehao Ren Zhuoran Shan Shaoqing Shen Minjun Shi Yimeng Song Mo Su Hoi Ping Suen Bo Sun Fangdi Sun Jian Sun lin Sun Wenyao Sun Tian Tian Xiaohua Tong Yihsing Tseng Ying Tu Hong Wang Lan Wang Xi Wan Zongming Wang Tinghai Wu Yaowen Xie Jian Yang Jun Yang Man Yuan Wenze Yue Hongda Zeng Kuo Zhang Neng Zhang Tao Zhang Yu Zhang Feng Zhao Yichen Zheng Qiming Zhou Nicholas Clinton Zhiliang Zhu Bing Xu Ministry of Education Key Laboratory for Earth System Modeling Department of Earth System ScienceTsinghua UniversityBeijing 100084China Tsinghua Urban Institute Tsinghua UniversityBeijing 100084China Center for healthy Cities Institute for China Sustainable UrbanizationTsinghua UniversityBeijing 100084China Department of Land Air and Water ResourcesUniversity of CaliforniaDavisCA 95616-8627USA Department of Geological and Atmospheric sciences Iowa State UniversityAmesIA 50011USA State Key Laboratory of Remote Sensing science Institute of Remote Sensing and Digital EarthChinese Academy of SciencesBeijing 100101China AI for Earth Laboratory Cross-Strait InstituteTsinghua UniversityBeijing 100084China International Institute for Earth System sciences Nanjing UniversityNanjing 210093China Research Center for Ecology and Environment of Central Asia Chinese Academy of SciencesUrumchi 830011China Key Laboratory of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China National Engineering Laboratory for Applied Technology in Forestry&Ecology in South china College of Life Science and TechnologyCentral South University of Forestry and TechnologyChangsha 410004China college of Surveying&Geo-Informatics Tongji UniversityShanghai 200092China Department of Urban Planning College of Architecture and Urban PlanningTongji UniversityShanghai 200092China Beijing Institute of Urban Planning Beijing 100045China Department of Urban Planning School of Urban DesignWuhan UniversityWuhan 430072China college of Earth sciences Jilin UniversityChangchun 130061China Research Center for Digital Mountain and Remote Sensing Application Institute of Mountain Hazards and EnvironmentChinese Academy of SciencesChengdu 610041China Institute of Remote Sensing and Geographic Information System School of Earth and Space SciencesPeking UniversityBeijing 100871China Department of Urban and Regional Planning School of Geography and PlanningSun Yat-sen Univ

Land use reflects human activities on *** land use is the highest level human alteration on Earth,and it is rapidly changing due to population increase and *** areas have widespread effects on local hydrology,climate,biodiversity,and food production[1,2].However,maps,that contain knowledge on the distribution,pattern and composition of various land use types in urban areas,are limited to city *** mapping standard on data sources,methods,land use classification schemes varies from city to city,due to differences in financial input and skills of mapping *** address various national and global environmental challenges caused by urbanization,it is important to have urban land uses at the national and global scales that are derived from the same or consistent data sources with the same or compatible classification systems and mapping *** is because,only with urban land use maps produced with similar criteria,consistent environmental policies can be made,and action efforts can be compared and assessed for large scale environmental ***,despite of the fact that a number of urban-extent maps exist at global scales[3,4],more detailed urban land use maps do not exist at the same *** at big country or regional levels such as for the United States,china and European Union,consistent land use mapping efforts are rare[5,6](e.g.,https://***/open_land_use/).

关键词： EULUC-china 土地利用类型

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九肋鳖形态及遗传特性分析

水生生物学报 2023年第9期47卷 1534-1544页

作者：万刚曾嘉伟肖何威熊钢靳利忠王晓清胡亚洲湖南农业大学动物科学技术学院长沙410128 湖南生物机电职业技术学院动物科学与技术系长沙410127

研究在湖南洞庭湖及沅江水域内开展中华鳖(Pelodiscus sinensis)种质资源调查,发现中华鳖中存在“九肋鳖”,为了解其资源分布、形态特征以及遗传特性,从形态指标、线粒体DNA及骨骼发育基因多态性入手,比较研究九肋鳖(9对肋骨,简称R9)与... 详细信息

研究在湖南洞庭湖及沅江水域内开展中华鳖(Pelodiscus sinensis)种质资源调查,发现中华鳖中存在“九肋鳖”,为了解其资源分布、形态特征以及遗传特性,从形态指标、线粒体DNA及骨骼发育基因多态性入手,比较研究九肋鳖(9对肋骨,简称R9)与八肋鳖(8对肋骨,简称R8)的形态及分子遗传特性。结果显示:(1)从湖南省沅江、益阳、常德和岳阳地区的12222只人工养殖中华鳖群体中共筛选出3.1只九肋鳖,其在养殖中华鳖群体中占比为2.2%—3.1%。(2)九肋鳖背甲内胸椎数为11枚,肋骨9对,比八肋鳖多1枚胸椎和1对肋骨。(3)九肋鳖背甲宽/背甲长、后侧裙边宽/背甲长显著小于八肋鳖,体高/背甲长显著大于八肋鳖。(4)九肋鳖CO I、Cytb、12S rRNA基因与中华鳖序列的同源性达到99%以上,进化树分析显示九肋鳖与中华鳖群体聚为一支,与中华鳖地理群体间均存在共享单倍型,未找到群体特异性标记。(5)九肋鳖与八肋鳖的RUNX2和VRTN基因外显子上共检测到4处突变位点,即g.977380 C>T、g.6014427C>T、g.6015734A>C及g.6015864A>C位点,且VRTN基因的g.6015864A>C突变与中华鳖胸椎数之间存在显著关联(P1枚胸椎和1对肋骨,VRTN基因g.6015864A>C位点可能与中华鳖多脊椎性状有关。研究结果对了解中华鳖种质特性及种业创新具有较重要的理论参考价值。

关键词：资源调查形态遗传特性种质创新九肋鳖

国内部分省市肉兔及蜱虫携带土拉杆菌的流行病学调查

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中国畜牧兽医 2020年第12期47卷 4069-4075页

作者：李书光王艺晖曲光刚孙翠平杨丽芳林初文沈志强何诚山东省滨州畜牧兽医研究院滨州256600 中国农业大学动物医学院北京100193 山东省滨州畜禽蜂胶疫苗研究开发推广中心滨州256600 山东绿都生物科技有限公司滨州256600

本调查旨在探明土拉杆菌在国内部分省份的流行情况。利用本实验室建立的土拉杆菌通用及亚种PCR检测研究方法,对国内肉兔饲养密度比较大的省份和牛羊存栏的部分省市开展了肉兔和蜱虫中土拉杆菌的检测,并对阳性样本进行了土拉杆菌亚种的... 详细信息

本调查旨在探明土拉杆菌在国内部分省份的流行情况。利用本实验室建立的土拉杆菌通用及亚种PCR检测研究方法,对国内肉兔饲养密度比较大的省份和牛羊存栏的部分省市开展了肉兔和蜱虫中土拉杆菌的检测,并对阳性样本进行了土拉杆菌亚种的鉴定和基因测序验证。研究结果表明,肉兔肝脏组织和牛羊携带蜱虫中土拉杆菌DNA检测阳性,其中218份肉兔样本中12份阳性(阳性率5.5%),490份蜱虫样本中15份阳性(阳性率3.1%);从地区分布来看,山东、河南、四川肉兔病料检测阳性,云南、山东蜱虫阳性率高;PCR检测显示其亚种为F.h,为土拉杆菌毒力较强的亚种。本次调查显示土拉杆菌在肉兔、蜱虫中存在,山东、云南地区存在土拉杆菌病公共卫生安全风险,应引起有关科研院校、医疗单位和政府部门的高度重视。

关键词：土拉杆菌肉兔蜱虫流行病学调查

基于细胞色素b和内转录间隔区基因序列的4个棕脊足螨地理种群遗传变异分析

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中国血吸虫病防治杂志 2023年第1期35卷 22-28页

作者：陶香林马飞李政阚心蕊叶长江孙恩涛皖南医学院检验学院安徽芜湖241002

目的分析不同地理种群棕脊足螨(Gohieria fusca)遗传多样性和遗传分化。方法自安徽省芜湖市(WH)、蚌埠市(BB)、亳州市(BZ)和浙江省嘉兴市(JX)等4个地区采集螨虫标本并筛选出棕脊足螨,采用PCR技术扩增棕脊足螨线粒体细胞色素b(cytochrome... 详细信息

目的分析不同地理种群棕脊足螨(Gohieria fusca)遗传多样性和遗传分化。方法自安徽省芜湖市(WH)、蚌埠市(BB)、亳州市(BZ)和浙江省嘉兴市(JX)等4个地区采集螨虫标本并筛选出棕脊足螨,采用PCR技术扩增棕脊足螨线粒体细胞色素b(cytochrome b,Cytb)和核糖体DNA内转录间隔区(internal transcribed spacer,ITS)基因并对扩增产物测序。采用Chromas 2和DNASTAR 1.00软件进行序列校对和拼接,采用DnaSP 5.10.00软件计算各种群单倍型多样性(Hd)、核苷酸多态性(Pi),应用MEGA 10.2软件包计算遗传分化指数(F_(st))和基因流(N_(m))。采用Arlequin 3.1软件计算Tajima’s D和Fu’s FS值,并进行中性检验和分子方差分析;采用Network 10.2软件基于Median-joining法构建单倍型网络图。结果棕脊足螨样本Cytb基因序列长度为372 bp、ITS基因序列长度为13.1~1320 bp。基于线粒体Cytb基因和核糖体ITS基因序列分析发现,4个棕脊足螨地理种群均表现出较高遗传多样性(Hd=0.804,Pi=0.00691)。分子方差分析发现,4个棕脊足螨地理种群间存在遗传分化(F_(st)=0.20240,P1)。基于Cytb基因的中性检验发现,棕脊足螨WH种群Tajima’sD值和Fu’s FS值分别为-1.79631和-3.29398(P均1197,具有较高遗传多样性。分子方差分析发现,棕脊足螨Fst值为0.10462(P20和-1.93599(P均>0.05),无明显历史扩张。结论4个棕脊足螨地理种群遗传多样性较高,存在显著遗传分化。由于不同棕脊足螨地理种群间局部发生高水平基因流,未发现其明显地理分布格局。

关键词：棕脊足螨细胞色素b 内转录间隔区种群多样性遗传分化基因流

基于SSR标记的西藏光核桃群体遗传多样性和遗传结构分析

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林业科学 2018年第2期54卷 30-41页

作者：包文泉乌云塔娜杜红岩李铁柱刘慧敏王淋白玉娥内蒙古农业大学林学院呼和浩特010018 中国林业科学研究院经济林研究开发中心郑州450003

【目的】利用SSR标记深入研究西藏光核桃的遗传多样性及遗传结构,揭示其遗传结构与地理分布、海拔梯度等的相关性,为西藏光核桃资源的有效利用与科学保护提供理论依据。【方法】利用25对SSR引物,分析西藏地区21个光核桃天然群体420份个... 详细信息

【目的】利用SSR标记深入研究西藏光核桃的遗传多样性及遗传结构,揭示其遗传结构与地理分布、海拔梯度等的相关性,为西藏光核桃资源的有效利用与科学保护提供理论依据。【方法】利用25对SSR引物,分析西藏地区21个光核桃天然群体420份个体的遗传多样性和遗传结构。应用Gen Al Ex 6.501、Arlequin v3.1、NTSYS pc version 2.10、STRUCTURE、STRUCTURE Harvester、CLUMP和Distruct等软件进行遗传参数估算、主坐标分析、遗传变异分析、聚类图构建及遗传结构分析。【结果】基于25个SSR分子标记的遗传多样性分析表明,西藏光核桃群体遗传多样性和近亲繁殖水平适中,其平均等位基因数(Na)、有效等位基因数(Ne)、期望杂合度(he)、观察杂合度(Ho)、Shannon’s信息指数(I)和近交系数(F)分别为3.8、2.5、0.52、0.44、0.95和0.17,其中,P17群体遗传多样性最高(Ne=4.7,he=0.63,Ho=0.56,I=1.57),而P18群体遗传多样性最低(Ne=1.7,he=0.30,Ho=0.22,I=0.49)。西藏光核桃的贝叶斯遗传结构分析(STRUCTURE)与遗传距离的主坐标分析(PCo A)、UPGMA聚类分析结果基本一致,均将供试420份光核桃个体划分为3个类群,其分组结果具有明显的地理区域特性。Mantel检测显示遗传距离与地理距离(r=0.50,P1)、海拔梯度(r=0.61,P1)呈显著正相关。分子方差分析(AMOVA)显示,16.3%的遗传变异来自群体间,群体间的遗传分化水平为中等,而大部分遗传变异(83.7%)来自群体内。【结论】西藏光核桃遗传多样性适中,群体间存在地理隔离效应和海拔梯度的遗传变异,其遗传分化程度较高,这可能源于西藏光核桃生境片段化、海拔梯度的影响以及山脉阻隔引起的地理隔离效应。西藏光核桃受人为干扰较严重,且个体间的近亲繁殖较频繁,若不及时采取保护措施,其遗传多样性将会逐渐降低。基于遗传结构分析,确定西藏光核桃3个保护单元,并建议限制人为活动对其破坏,实施就地保护的同时,促进不同居群间的基因交流,保护西藏光核桃的遗传多样性。

关键词：光核桃西藏遗传多样性遗传结构 SSR

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草原风蚀坑发育对土壤生态化学计量的影响

中国沙漠 2023年第5期43卷 166-175页

作者：李雨薇王博包玉海韩彦隆闫茂林王伟峰内蒙古师范大学地理科学学院内蒙古呼和浩特010022 内蒙古农业大学沙漠治理学院内蒙古呼和浩特010018 内蒙古自治区林业科学研究院内蒙古呼和浩特010010

为了探讨草原风蚀坑发育对土壤碳氮磷含量特征的影响,通过时空替代法分析土壤碳氮磷含量、储量及生态化学计量比的时空分异特征及环境响应。结果表明:(1)风蚀坑发育导致土壤含水率、田间持水量、总孔隙度分别下降57.8%、23.2%、15.4%,... 详细信息

为了探讨草原风蚀坑发育对土壤碳氮磷含量特征的影响,通过时空替代法分析土壤碳氮磷含量、储量及生态化学计量比的时空分异特征及环境响应。结果表明:(1)风蚀坑发育导致土壤含水率、田间持水量、总孔隙度分别下降57.8%、23.2%、15.4%,而土壤pH值和容重分别上升12.4%和13.7%,砂粒含量增加35.8%,粉粒和黏粒含量分别降低69.4%和79.6%;(2)风蚀坑发育造成土壤有机碳含量和储量分别降低34.9%和20.0%,0~20 cm土壤碳流失量最大,土壤氮含量与储量分别降低14.6%和3.1%,60~80 cm土壤氮流失量最显著,土壤磷含量和储量分别降低3.1%和3.4%,20~40 cm土壤磷流失量最多;(3)风蚀坑发育造成土壤C/N、C/P和N/P分别下降24.2%、34.5%和6.9%,表层0~20 cm土壤化学计量比降低最显著;(4)风蚀坑发育对土壤粒径组成的改变是引起土壤碳氮磷含量变化最主要因子,土壤水分供给能力的降低是次要因素。草原风蚀坑发育会造成土壤碳氮磷养分的大量流失,并导致土壤粗粒化、干旱化及次生盐渍化现象。

关键词：土壤碳氮磷生态化学计量比空间分布风蚀坑