检索结果-南通市图书馆

Combined application of Trichoderma harzianum SH2303 and difenoconazole-propiconazolein controlling Southern corn leaf blight disease caused by Cochliobolus heterostrophus in maize

引用

Journal of Integrative Agriculture 2019年第9期18卷 2063-2071页

作者： WANG Shao-qing MA Jia WANG Meng WANG Xin-hua li ya-qian CHEN Jie School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240P.R.China Key Laboratory of Urban Agriculture(South)Ministry of Agriculture Shanghai Jiao Tong UniversityShanghai 200240P.R.China State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai 200240P.R.China Plant Genetic Engineering Center of Hebei Province Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051P.R.China

Southern corn leaf blight(SCLB)disease caused by Cochliobolus heterostrophus is one of the major threats to corn production *** synergistic application of low toxic chemical fungicide and biocontrol agents could improve biocontrol stability and efficiency against plant diseases,which ultimately reduce use of chemical *** spp.,well-known biocontrol fungi have been used to control some foliar ***,few works have been reported on synergistic application of chemical fungicide and Trichoderma against foliar *** study was aimed to investigate the control effect on the synergistic application of Trichoderma harzianum SH2303 and difenoconazole-propiconazole(DP)against *** showed that the synergistic application of DP and SH2303 reduced the leaf spot area compared to the *** efficacy of synergistic application of DP+SH2303 against SCLB could last for 15–20 d in pot trial under the greenhouse *** the natural field condition,maize treated with DP+DP and DP+SH2303 showed 60%control,which was higher than that of SH2303+DP(45%)and SH2303+SH2303(35%).All these treatments induced the synthesis of defense-related enzymes(phenylalanine ammonia lyase(PAL),catalase(CAT),and superoxide dismutase(SOD))and the defence-related gene expression of SA pathway(PR1).Taken together the in-vitro leaf test and field trial,the control of SCLB by synergistic application of DP+SH2303 was similar to that of DP+*** synergistic application,the sequential application of DP+SH2303 showed better control than the sequential application of SH2303+*** was concluded that the synergistic application of chemical fungicide(DP)and biocontrol agent(*** SH2303)could be used to reduce the chemical fungicide and to reduce the SCLB diseases in maize,which provided alternative approach to realize an eco-friendly controlling of the foliar disease.

关键词： synergistic bio-control chemical fungicide Trichoderma harzianum maize leaf spot disease

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Degradation effects on dichlorvos by a biocontrol strain,Trichoderma atroviride T23

引用

Journal of Integrative Agriculture 2023年第9期22卷 2746-2758页

作者： SUN Jia-nan SI Gao-yue liU Hong-yi li ya-qian WANG Xin-hua CHEN Jie School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240P.R.China State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai 200240P.R.China

Excessive use of organophosphate pesticides(OP),such as dichlorvos,in farming system poses a threat to human health through potential contamination of *** date,biodegradation has been prospected most promising approach to eliminate environmental OP *** species as a biological control microorganism is often exposed to the chemical pesticides applied in environments,so it is necessary to understand the mechanism of degradation of dichlorvos by *** this study,dichlorvos significantly inhibited the growth,sporulation and pigmentation of *** T23,and the dichlorvos degradation activity of T23 required the initial induction effect of dichlorvos and the culture conditions,including the nutrient and pH values of the *** changed primary and secondary metabolites released from T23 in the presence of dichlorvos were speculated as the energy and antioxidants for the strain itself to tolerate dichlorvos *** results showed that T23 could produce a series of enzymes,especially the intracellular enzymes,to degrade *** activities of the intracellular enzyme generated by T23 were differentially changed along time course and especially relied on initial dichlorvos concentration,ammonium sulfate and phosphate added in the *** conclusion,some dichlorvos-induced chemical degradation related enzymes of T23 were proved to be involved in the degradation of dichlorvos.

关键词： Trichoderma atroviride T23 dichlorvos intracellular enzyme induced enzyme activity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MAPKs and acetyl-CoA are associated with Curvularia lunata pathogenicity and toxin production in maize

引用

Journal of Integrative Agriculture 2018年第1期17卷 139-148页

作者： NI Xuan GAO Jin-xin YU Chuan-jin WANG Meng SUN Jia-nan li ya-qian CHEN Jie School of Agriculture and Biology Shanghai Jiao Tong University/State Key Laboratory of Microbial Metabolism Shanghai JiaoTong UniversityKey Laboratory of Urban Agriculture (South) Ministry of Agriculture Shanghai 200240 P.R.China

Mitogen-activated protein kinase （MAPK） cascades play an important role in extracellular signal transduction and are involved in the pathogenicity of fungal pathogens to host plants. In Curvularia lunata, the roles of two MAPK genes, Clkl and CIm 1, have already been studied. Clkl is involved in conidia formation and pathogenicity, and Clmf is closely related to pathogen cell wall formation and pathogenicity to maize leaves. In this study, a third C. lunata MAPK gene, Clhl, which is homologous to hog1, was successfully cloned. We found that a Clhl deletion mutant had lower intracellular glycerol accumulation than the wild-type stain and was unable to grow normally under osmotic stress conditions. Furthermore, the deletion mutants of three C. lunata MAPK genes （Clkl, Clml and Clhl） had lower levels of acetyI-CoA, which is an important intermediate product in the synthesis of melanin and furan toxin, and down-regulated expression of pathogenicity-associated genes. Furthermore, pathogenicity and the ability to produce toxin were restored after adding acetyI-CoA to the culture medium, suggesting that acetyI-CoA is closely involved in the pathogen MAPK signaling pathway.

关键词： Curvularia lunata MAP kinase acetyI-CoA pathology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Screening of antagonistic Trichoderma strains and their application for controlling stalk rot in maize

引用

Journal of Integrative Agriculture 2020年第1期19卷 145-152页

作者： LU Zhi-xiang TU Guang-ping ZHANG Ting li ya-qian WANG Xin-hua Zhang Quan-guo SONG Wei CHEN Jie School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240P.R.China Plant Protection College Shenyang Agricultural UniversityShenyang 110866P.R.China State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai 200240P.R.China Key Laboratory of Urban Agriculture Ministry of AgricultureShanghai 200240P.R.China Institute of Cereal and oil Crops Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051P.R.China

Maize is one of the major crops in China, but maize stalk rot occurs nationwide and has become one of the major challenges in maize production in China. In order to find an environment-friendly and feasible technology to control this disease, a Trichoderma-based biocontrol agent was selected. Forty-eight strains with various inhibition activities to Fusarium graminearum, and Fusarium verticillioides were tested. A group of Trichoderma strains(DLY31, SG3403, DLY1303 and GDFS1009) were found to provide an inhibition rate to pathogen growth in vitro of over 70%. These strains also prevented pathogen infection over 65% and promoted the maize seedling growth for the main root in vivo by over 50%. Due to its advantage in antifungal activity against pathogens and promotion activity to maize, Trichoderma asperellum GDSF1009 was selected as the most promising strain of the biocontrol agent in the Trichoderma spectrum. Pot experiments showed that the Trichoderma agent at 2–3 g/pot could achieve the best control of seedling stalk rot and promotion of maize seedling growth. In the field experiments, 8–10 g/hole was able to achieve over 65% control to stalk rot, and yield increased by 2–11%. In the case of natural morbidity, the control efficiency ranged from 27.23 to 48.84%, and the rate of yield increase reached 11.70%, with a dosage of Trichoderma granules at 75 kg ha^-1. Based on these results, we concluded that the Trichoderma agent is a promising biocontrol approach to stalk rot in maize.

关键词： stalk rot in maize biocontrol Trichoderma Fusarium granules

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Sod gene of Curvularia lunata is associated with the virulence in maize leaf

引用

Journal of Integrative Agriculture 2017年第4期16卷 874-883页

作者： GAO Shi-gang NI Xuan li Ying-ying FU Ke-he YU Chuan-jin GAO Jin-xin WANG Meng li ya-qian CHEN Jie School of Agriculture and Biology Shanghai Jiao Tong University/State Key Laboratory of Microbial MetabolismShanghai Jiao Tong University/Key Laboratory of Urban Agriculture(South)Ministry of Agriculture School of Biology Nanchang Normal University

Curvularia leaf spot, caused mainly by Curvularia lunata, is a widespread plant disease in China. In the recent years, di- rectional host selection by the pathogen, which likely results in the virulence differentiation in pathogens, is widely reported. Among the hallmarks potentially associated to pathogen variation in virulence, superoxide dismutase gene Sod has been found to be closely related to the enhancement of virulence. In the present study, the full-length of Sod was obtained via Blastn alignment against GenBank and the whole genome of C. lunata. In order to understand the role of Sod in the vir- ulence variation in C. lunata, targeted gene disruption was performed to construct Sod mutants. The cell wall degrading enzyme （CWDE） activities and toxin production of ASod were not distinctly different from wild-type strain CX-3 and its complon. However, at an early stage of infection, 3Sod virulence appeared to be lower than CX-3 and the complon, while at a later stage, its virulence gradually returned to the level of CX-3 and the complon. Furthermore, the melanin production of ASod was significantly reduced compared to CX-3 and the complon, suggesting that Sod gene influences the virulence by regulating melanin production at an early stage of infection but is not essential for pathogenicity. However, the disruption of Sod did not significantly affect the transcriptional expression of the melanin biosynthesis-associated genes, bml and scd. Therefore, we infer that Sod in C. lunata are involved, to some extent, with the virulence in maize leaf, but still needs further studies to have a clear understanding of its mechanism.

关键词： Curvularia lunata Sod gene function infection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nonlinear parametrically excited vibration and active control of gear pair system with time-varying characteristic

引用

Chinese Physics B 2015年第10期24卷 303-311页

作者：刘爽王进进刘金杰李雅倩 Key Laboratory of Industrial Computer Control Engineering of Hebei Province Yanshan University National Engineering Research Center for Equipment and Technology of Cold Strip Rolling Yanshan University

In the present work, we investigate the nonlinear parametrically excited vibration and active control of a gear pair system involving backlash, time-varying meshing stiffness and static transmission error. Firstly, a gear pair model is established in a strongly nonlinear form, and its nonlinear vibration characteristics are systematically investigated through different approaches. Several complicated phenomena such as period doubling bifurcation, anti period doubling bifurcation and chaos can be observed under the internal parametric excitation. Then, an active compensation controller is designed to suppress the vibration, including the chaos. Finally, the effectiveness of the proposed controller is verified numerically.

关键词： nonlinear parametrically excited vibration time-varying meshing stiffness static transmission er-ror chaos

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

超连续谱激光在湍流大气中传输特性的数值仿真研究

引用

光子学报 2019年第10期48卷 111-119页

作者：李雅倩朱文越钱仙妹中国科学院安徽光学精密机械研究所中国科学院大气光学重点实验室合肥230031 中国科学技术大学研究生院科学岛分院合肥230026

采用多层相位屏的数值仿真方法,综合考虑大气衰减效应和湍流效应,研究了不同湍流强度和不同源相干度条件下超连续谱激光的光束扩展、光斑漂移以及闪烁特性,并对比分析了超连续谱激光传输与单色激光传输特性的差异.结果表明:湍流越强或... 详细信息

采用多层相位屏的数值仿真方法,综合考虑大气衰减效应和湍流效应,研究了不同湍流强度和不同源相干度条件下超连续谱激光的光束扩展、光斑漂移以及闪烁特性,并对比分析了超连续谱激光传输与单色激光传输特性的差异.结果表明:湍流越强或源相干度越好,超连续谱激光的相对光束扩展越严重;湍流愈强,光斑漂移愈严重,但不同源相干度的超连续谱激光的质心漂移方差几乎相等;降低超连续谱激光的源相干度可以在一定程度上减弱光强起伏的闪烁效应,并降低闪烁指数对离轴距离的依赖性;与单色激光相比,湍流对超连续谱激光的光束扩展以及闪烁效应影响较弱,而质心漂移效应两者差异不明显.单色激光大气传输的数值仿真结果与理论解析结果基本吻合,在一定程度上说明了数值仿真过程的可靠性.该研究不仅提出了一种获得超连续谱激光大气传输特性规律的有效仿真手段,而且为超连续谱激光传输的工程应用提供参考.

关键词：超连续谱激光传输数值仿真光斑漂移光束扩展大气湍流光强闪烁指数

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

能量异构的无线传感器网络加权无标度拓扑研究

引用

物理学报 2014年第15期63卷 34-41页

作者：韩丽刘彬李雅倩赵磊静燕山大学信息科学与工程学院秦皇岛066004 河北省特种光纤与光纤传感重点实验室秦皇岛066004

针对无线传感器网络节点能耗不均和如何高效获得节点和边的负载问题,提出一种局域范围内能量异构的加权无标度拓扑演化模型.通过对节点能量与负载、能耗的关系建模,建立节点能量与点权和边权的联系,进而结合点权和加权模型给出网络的演... 详细信息

针对无线传感器网络节点能耗不均和如何高效获得节点和边的负载问题,提出一种局域范围内能量异构的加权无标度拓扑演化模型.通过对节点能量与负载、能耗的关系建模,建立节点能量与点权和边权的联系,进而结合点权和加权模型给出网络的演化方式,推出点权、度和边权的幂率分布规律,最终根据网络获得的点权和边权来分析负载和能耗.仿真结果表明,提出的模型不仅能够准确计算点边的负载,而且缓解了无标度网络的节点能耗不均衡问题.

关键词：无线传感器网络加权能耗均衡负载

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CCD分段测量的光学位移测量系统

引用

光学精密工程 2011年第9期19卷 2036-2042页

作者：李雅倩付献斌周坤燕山大学电气工程学院河北秦皇岛066004

为了高精度地实现大量程的位移测量,采用传统激光三角法的测量原理,提出一种基于虚拟探测器的激光三角法来测量位移。该方法基于3个CCD分段测量的思想,将3个CCD互相独立且沿光轴均匀分布,以此扩大传统方法测量的范围。使用平面反射镜作... 详细信息

为了高精度地实现大量程的位移测量,采用传统激光三角法的测量原理,提出一种基于虚拟探测器的激光三角法来测量位移。该方法基于3个CCD分段测量的思想,将3个CCD互相独立且沿光轴均匀分布,以此扩大传统方法测量的范围。使用平面反射镜作为虚拟探测器进行探测,当成像光束经平面镜反射后在CCD上成像时,系统相当于增加了一个CCD,扩展了测量量程。由准直系统、偏振片、光阑组成准直滤光系统,缩小探测器光敏面上像点的直径,减小被测表面非理想光点对测量精度的影响,从而实现了较高精度下的大位移测量。最后通过实验验证了方法的可行性。

关键词：激光三角法 CCD 虚拟探测器位移测量

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

回转体形貌测量中的相机自标定

引用

光学精密工程 2011年第8期19卷 1957-1963页

作者：李雅倩林洪彬燕山大学电气工程学院河北秦皇岛066004

为实现回转体形貌测量系统的相机自标定,研究了回转体对象的透视投影特性,提出了一种基于被测回转体对象自约束特征的摄像机内、外参数自动标定方法。首先,利用被测场景中回转体截面特征易于提取的特性,从测得图像提取场景中的截面椭圆... 详细信息

为实现回转体形貌测量系统的相机自标定,研究了回转体对象的透视投影特性,提出了一种基于被测回转体对象自约束特征的摄像机内、外参数自动标定方法。首先,利用被测场景中回转体截面特征易于提取的特性,从测得图像提取场景中的截面椭圆,利用正交于回转轴的截面椭圆求取圆环点的像,并结合极线约束建立绝对二次曲线像的全约束方程;然后,通过对求取的绝对二次曲线矩阵进行Cholesky分解获得相机的内参数。其次,在求得的内参数矩阵的基础上,通过回转轴和截面图像的成像特点建立测量系统外参数方程,求解测量系统外部参数。实验结果表明,利用本文方法进行相机内参数标定的精度优于0.95%,外参数标定精度优于5.6%。该方法只需利用被测对象自身几何约束,不需任何标定板和标定块,标定过程简单有效,能够满足工程实际需要。

关键词：形貌测量回转体标定圆环点极线约束

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：