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Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria

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Journal of Oceanology and limnology 2020年第2期38卷 364-377页

作者： ZHANG Chan ZHANG Wei-jia YIN Qunjian li xuegong QI Xiaoqing WU Long-fei Laboratory of Deep Sea Microbial Cell Biology Institute of Deep-sea Science and EngineeringChinese Academy of SciencesSanya 572000China University of Chinese Academy of Sciences Beijing 100049China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms CNRS-Marseille/CAS-SanyaChina Aix Marseille Univ CNRSLCBMarseille F-13402France

Trimethylamine N-oxide(TMAO)is one of the most important nutrients for bacteria in the deep-sea environment and is capable of improving pressure tolerance of certain bacterial *** assess the impact of TMAO on marine microorganisms,especially those dwelling in the deep-sea environment,we analyzed the bacterial community structure of deep-sea sediments after incubated under different *** at 50 MPa and 0.1 MPa revealed that TMAO imposed a greater influence on bacterial diversity and community composition at atmospheric pressure condition than that under high hydrostatic pressure(HHP).We found that pressure was the primary factor that determines the bacterial ***,in total,238 bacterial strains were isolated from the enrichments,including 112 strains a ffiliated to 16 genera of 4 phyla from the Yap Trench and 126 strains a ffiliated to 11 genera of 2 phyla from the Mariana *** of HHP reduced both abundance and diversity of isolates,while the presence of TMAO mainly af fected the diversity of isolates *** addition,certain genera were isolated only when TMAO was *** together,we demonstrated that pressure primarily defines the bacterial community and culturable bacterial ***,we showed for the first time that TMAO had distinct influences on bacterial community depending on the pressure *** results enriched the understanding of the significance of TMAO in bacterial adaptation to the deep-sea environment.

关键词： deep-sea bacteria high hydrostatic pressure(HHP) trimethylamine N-oxide(TMAO) community structure

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Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

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Journal of Oceanology and limnology 2019年第1期37卷 210-222页

作者： YIN Qunjian ZHANG Weijia li xuegong ZHOU lihong QI Xiaoqing ZHANG Chan WU Long-Fei Laboratory of Deep-sea Microbial Cell Biology Institute of Deep-sea Science and Engineering Chinese Academy of Sciences University of Chinese Academy of Sciences International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms CNRS-Marseille/CAS-Beijing-Qingdao-Sanya CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions Institute of Deep-sea Science and Engineering Chinese Academy of Sciences AMU LCB UMR 7283 CNRS-Marseille

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.

关键词： marine bacteria Trimethylamine N-oxide(TMAO) high hydrostatic pressure(HHP) pressure tolerance phenotype

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Complicated evolutionary patterns of microRNAs in vertebrates

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Science China(life Sciences) 2008年第6期51卷 552-559页

作者： WANG XiaoWo, ZHANG xuegong & li YanDa MOE Key Laboratory of Bioinformatics and Bioinformatics Division, TNliST/Department of Automation, Tsinghua University, Beijing 100084, China 1. MOE Key Laboratory of Bioinformatics and Bioinformatics Division TNLIST / Department of Automation Tsinghua University Beijing 100084 China

MicroRNAs (miRNAs) are a class of ～22 nt long endogenous non-coding RNAs that play important regulatory roles in diverse organisms. Up to now, little is known about the evolutionary properties of these crucial regulators. Most miRNAs were thought to be phylogenetically conserved, but recently, a number of poorly-conserved miRNAs have been reported and miRNA innovation is shown to be an ongoing process. In this work, through the characterization of an miRNA super family, we studied the evolutionary patterns of miRNAs in vertebrates. Recently generated miRNAs seem to evolve rapidly during a certain period following their emergence. Multiple lineage-specific expansions were observed. Homolgous premiRNAs may produce mature products from the opposite stem arms following tandem duplications, which may have important contribution to miRNA innovation. Our observations of miRNAs' complicated evolutionary patterns support the notion that these key regulatory molecules may play very active roles in evolution.

关键词： microRNA evolution miRNA:miRNA* interconversion lineage specific duplication

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Synergistic teratogenic effects of hyperthermia and Thiadiazole

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吉林大学学报(医学版) 1994年第6期 574-576页

作者： He Weigong,li Helian,Wang xuegong and li shourou(Department of Obstetrics and Gynecology,Second Teaching Hospital)

ＳｙｎｅｒｇｉｓｔｉｃｔｅｒａｔｏｇｅｎｉｃｅｆｒｅｃｔｓｏｆｈｙｐｅｒｔｈｅｒｍｉａａｎｄＴｈｉａｄｉａｚｏｌｅＨｅＷｅｉｇｏｎｇ，ＬｉＨｅｌｉａｎ，ＷａｎｇＸｕｅｇｏｎｇａｎｄＬｉｓｈｏｕｒｏｕ（Ｄｅｐａｒｔｍｅ... 详细信息

ＳｙｎｅｒｇｉｓｔｉｃｔｅｒａｔｏｇｅｎｉｃｅｆｒｅｃｔｓｏｆｈｙｐｅｒｔｈｅｒｍｉａａｎｄＴｈｉａｄｉａｚｏｌｅＨｅＷｅｉｇｏｎｇ，ＬｉＨｅｌｉａｎ，ＷａｎｇＸｕｅｇｏｎｇａｎｄＬｉｓｈｏｕｒｏｕ（ＤｅｐａｒｔｍｅｎｔｏｆＯｂｓｔｅｔｒｉｃｓａｎｄ...

关键词： hyperthermia induced/adv eff Thiadiaole/adv eff teratogens/tox

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Resazurin as an indicator of reducing capacity for analyzing the physiologic status of deep-sea bacterium Photobacterium phosphoreum ANT-2200

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Journal of Oceanology and limnology 2021年第1期39卷 297-305页

作者： Jie DAI Hongzhi TANG xuegong li Claire-lise SANTIN Wenpeng CUI Na liU Xiaoqing QI Xuehua CUI Alain GROSSI Philippe NOTARESCHI Wei-Jia ZHANG Long-Fei WU Laboratory of Deep Sea Microbial Cell Biology Institute of Deep Sea Science and EngineeringChinese Academy of SciencesSanya 572000China University of Chinese Academy of Sciences Beijing 100049China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms(liA-MagMC) CNRS-CAS Aix Marseille University CNRSLCBIMMFR3479IM2BMarseille 13402France Aix Marseille University CNRSIMMFR3479Marseille 13402France

Resazurin(RZ)is a weakly fl uorescent blue dye and can be reduced irreversibly to highly fl uorescent pink resorufi n(RF)that is reduced reversibly to colorless dihydroresorufi n(hRF)by photodeoxygenation,chemical reaction and reductive organic compounds produced through cell *** of the reliable and sensitive fl uorescence-color change and noninvasive features,RZ has been used widely as a redox indicator in cell viability/proliferation assays for bacteria,yeast,and mammalian ***,RZ is used rarely for physiological characterization of marine ***,we developed a custom-made irradiation and absorption-analysis device to assess the reducing capacity and physiologic status of marine bacterial *** measured the absorption spectra of RZ,RF,and hRF in the presence of the reducing compound Na 2 S and under visible-light *** establishing appropriate parameters,we monitored the color changes of RZ and its reduced derivatives to evaluate the coherence between reducing capacity,bioluminescence and growth of the deep-sea bacterium Photobacterium phosphoreum strain ANT-2200 under various *** of bioluminescence is an oxidation process dependent upon cellular reducing *** and bioluminescence of ANT-2200 cell cultures were impeded progressively with increasing concentrations of RZ,which suggested competition for reducing molecules between RZ at high concentration with reductive ***,caution should be applied upon direct addition of RZ to growth media to monitor redox reactions in cell *** of the instantaneous reduction velocity of RZ in ANT-2200 cell cultures showed a detrimental eff ect of high hydrostatic pressure and high coherence between the reducing capacity and bioluminescence of *** data clearly demonstrate the potential of using RZ to characterize the microbial metabolism and physiology of marine bacteria.

关键词： oxic-reduction indicator absorption spectra bacterial growth marine microorganism

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转捩:革故鼎新的战国制度

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中国史研究动态 2021年第5期 53-57页

作者：李学功湖州师范学院湖州发展研究院浙江湖州313000

在中国历史长河中,如果要选出影响并铸就中国风格、中国精神、中国气质的文化奠基期,毫无疑问这个时代非"战国"莫属,尤其是在今天"何以中国"的话题讨论下更彰显重要的意义。战国之所以令人念兹在兹,影响一代代后来者,除却一种铭心刻骨... 详细信息

在中国历史长河中,如果要选出影响并铸就中国风格、中国精神、中国气质的文化奠基期,毫无疑问这个时代非"战国"莫属,尤其是在今天"何以中国"的话题讨论下更彰显重要的意义。战国之所以令人念兹在兹,影响一代代后来者,除却一种铭心刻骨的血缘底色和文化情愫.

关键词：话题讨论文化情愫革故鼎新战国

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闻道与问道:中国古代社会形态问题研究的思考

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史学理论研究 2021年第2期 31-35页

作者：李学功湖州师范学院湖州发展研究院

中国自古就有"闻道"与"问道"的传统。综观人类历史,不难发现,文化创新与理论创造其实就是在不间断的"闻道"过程中展开的,也是经由"闻道"的洗礼,进而展开"问道"的探究,逐渐丰富社会认知,升华价值思考。由此出发,中国古代社会形态问题的... 详细信息

中国自古就有"闻道"与"问道"的传统。综观人类历史,不难发现,文化创新与理论创造其实就是在不间断的"闻道"过程中展开的,也是经由"闻道"的洗礼,进而展开"问道"的探究,逐渐丰富社会认知,升华价值思考。由此出发,中国古代社会形态问题的认识与研究亦可作如是观。

关键词：中国古代社会形态闻道社会认知不间断

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论《越绝书》的文化整合意识

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浙江社会科学 2008年第10期 94-98页

作者：李学功湖州师范学院人文学院教授湖州313000

《越绝书》作为越地越人的精神文化遗产,它以宝贵的文本载体形式,记录了大转折时代——春秋之于越的历史。对于《越绝书》,从作者、版本、内容等,前贤近哲多有研究,成绩斐然。笔者关注的是《越绝书》所透露出的文化整合意识。认为,作为... 详细信息

《越绝书》作为越地越人的精神文化遗产,它以宝贵的文本载体形式,记录了大转折时代——春秋之于越的历史。对于《越绝书》,从作者、版本、内容等,前贤近哲多有研究,成绩斐然。笔者关注的是《越绝书》所透露出的文化整合意识。认为,作为一部最早的地方区域史著,《越绝书》既有彰显越国文化精神的一面,也有吸收、认同儒家思想为代表的中原主流文化的一面,其间透射着以主流文化价值观整合越地思想文化的认识与努力。

关键词：文化整合剑与书仇与德

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重大突发事件下应急冷链供应链协同化评价模型及策略研究

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中国安全生产科学技术 2021年第10期17卷 18-24页

作者：李学工李芳曲阜师范大学(日照校区)管理学院山东日照276826

为有效规避冷链供应链受突发事件带来的不利影响,并强化其应急体系协同化发展,提出基于层次分析法和序参量法的应急冷链供应链协同化水平评价模型。模型以供应链协同化理论为基础,从协同化目标入手构建影响其协同化水平的测度指标体系;... 详细信息

为有效规避冷链供应链受突发事件带来的不利影响,并强化其应急体系协同化发展,提出基于层次分析法和序参量法的应急冷链供应链协同化水平评价模型。模型以供应链协同化理论为基础,从协同化目标入手构建影响其协同化水平的测度指标体系;利用层次分析法确定评价指标的综合权重,最后运用序参量法评估冷链供应链各应急环节的协同度。结果表明:评价模型能有效评估应急冷链供应链协同化水平,可为冷链供应链应对突发事件提供参考。

关键词：重大突发事件应急冷链供应链层次分析法(AHP) 序参量协同化策略

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从“裁辅天地”到“天人合一”:中华生态文化的思想域分

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湖州师范学院学报 2018年第1期40卷 1-6页

作者：李学功湖州师范学院湖州发展研究院浙江湖州313000

中华生态文化与思想厚积于农耕文明的深厚土壤,薄发于当下贞下起元的新时代,立基点始终不脱天地人关系的协调、进步与发展。自《周易》提出"裁辅天地"的生态协调论,至道家老庄一脉的"道法自然"论,到儒家荀子的"天人相分"论,以及邹衍的"... 详细信息

中华生态文化与思想厚积于农耕文明的深厚土壤,薄发于当下贞下起元的新时代,立基点始终不脱天地人关系的协调、进步与发展。自《周易》提出"裁辅天地"的生态协调论,至道家老庄一脉的"道法自然"论,到儒家荀子的"天人相分"论,以及邹衍的"天人合一"论。不难发现,传统生态思想与文化的发端始自轴心时代的思想家和社会,存录于先秦秦汉文献典籍中。历史不断地证明,实现进步的关键是新思想的引领。中华生态文化的创造性、传承性和天下意识,在历史的节点折射出思想的域分驰张,隐含着人与自然生命共同体的思考指向。

关键词：生态思想裁辅天地道法自然天人相分天人合一

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