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A Preliminary Study of The Short Circuit Current(I_(sc)) Responses of Endometria from Estrum and Bilateral Ovariectomy Female Rats

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Journal of Reproduction and Contraception 2010年第4期21卷 203-212页

作者： Jing-dong XU li-sheng li Hua GUO xiao-li HOU Yue ZhANG xiao-suo FU Jin-xia ZHU Department of Physiology and pathophysiology Basic Medical College Capital Medical University Beijing 100069 China

Objective To investigate the property of the endometrium ion transport in the normal estruation and ovariectomize rats. Methods The basic electrophysiological property of the endometrium was carried out by mean of the short circuit current （Isc） recording with the rats. Results The baseline Lsc and the transepithelial resistance （Rte） were different between the estruation and ovariectomized groups. Apical application of Na＋ channel blocker, amiloride （10 μmol/L）, or CFTR Cl- channel agonist, forskolin （10 μmol/L） did not significantly affect the Lc in the two groups. However, the 1sc in the model group was more sensitive to the CFTR Cl- channel blocker （glibenclamide, 1 mmol/L, apical） decreased about 27.0% （P〈0.05, n=6）, no remarkably changes in the estruation rats. While apical application CA CC CI-channel blocker, DIDS （4, 4 ＇-diisothiocyanostilbene- 2,2 ＇-disulfonic, 100 μmol/L）, the Isc was much more sensitive, percentage almost to 36.52% （P〈0.01） in the model group than that in the control Furthermore, basolateral application of bumetanide （100μmol/L）, a blocker of Na＋-K＋-2Cl- cotransport, didn＇t significantly reduce the Lc in the two groups. Conclusion The baseline Lsc and membrane resistance in the ovariectomize group is much higher than that in the normal estruation group, and the endometrial epithelium CI- secretion may be mediated by predominantly calcium-dependent CI- channels （CACC） and activating adenylate cyclase and apical cAMP-dependent Cl- channels （CFTR） in the model group with minor contributions in the control. Furthermore, the endometrial epithelium responses to different stimulants exists difference in the control and model group a likely mechanism for the ovary hormone.

关键词： endometrial epithelium short-circuit current ovariectomized rat

Characterization and map-based cloning of miniature2-m1, a gene controlling kernel size in maize

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Journal of Integrative Agriculture 2020年第8期19卷 1961-1973页

作者： GUAN Hai-ying dong Yong-bin LU Shou-ping liU Tie-shan HE Chun-mei liU Chun-xiao liU Qiang dong Rui WANG Juan li Yu-ling QI Shi-jun WANG li-ming Maize Research Institute Shandong Academy of Agricultural Sciences/National Engineering Laboratory of Wheat and Maize/Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-Huai River PlainMinistry of AgricultureJinan 250100P.R.China College of Agronomy Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops/National Key Laboratory of Wheat and Maize Crop ScienceZhengzhou 450046P.R.China

Kernel development plays an important role in determining kernel size in *** we present the cloning and characterization of a maize gene,nitrate transporter1.5(NRT1.5),which controls small kernel phenotype by playing an important role in kernel development.A novel recessive small kernel mutant miniature2-m1(mn2-m1)was isolated from self-pollinated progenies of breeding *** mutant spontaneously showed small kernel character arresting both embryo and endosperm development at an early stage after *** 21 polymorphic SSR markers,the mn2-m1 locus was limited to a 209.9-kb interval using 9176 recessive individuals of a BC1 segregating population from mn2-m1/*** one annotated gene was located in this 209.9 kb region,Zm00001 d019294,which was predicted to encode nitrate transporter1.5(NRT1.5).Allelism tests confirmed that mn2-m1 was allelic to miniature2-m2(mn2-m2)and miniature2-710 B(mn2-710 B).The mn2-m1 and mn2-m2 alleles both had nucleotide deletions in the coding region resulting in premature termination,and the mn2-710 B allele had some missence *** localization showed that Miniature 2(MN2)is localized in the plasma *** real-time PCR(qRT-PCR)analysis revealed that the expression of MN2 and some genes involved in the basal endosperm transfer layer(BETL)and embryo surrounding region(ESR)development were affected in mn2-m1 *** results suggested that MN2 plays an important role in maize seed development.

关键词： miniature2-m1 nitrate transporter1.5 frame shift mutation allelism tests subcellular localization

Two Avowable Quantum Communication Schemes

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Communications in Theoretical Physics 2008年第11期50卷 1109-1112页

作者： YAN Feng-li DING dong College of Physics Science and Information Engineering Hebei Normal University Shijiazhuang 050016 China North China Institute of Science and Technology Langfang 101601 China

Two avowable quantum communication schemes are *** is an avowable teleportation protocolbased on the quantum *** this protocol one teleports a set of one-particle states based on the availabilityof an honest arbitrator,the keys and the Einstein-Podolsky-Rosen pairs shared by the communication parties and *** key point is that the fact of the teleportation can neither be disavowed by the sender nor be denied bythe *** is an avowable quantum secure direct communication scheme.A one-way Hash function chosenby the communication parties helps the receiver to validate the truth of the information and to avoid disavowing for thesender.

关键词： avowable teleportation avowable quantum secure direction communication EPR pairs Hash function

Characterization of the protein expression and localization of hnRNP family members during murine spermatogenesis

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Asian Journal of Andrology 2023年第3期25卷 314-321页

作者： xiao-li Wang Jin-Mei li Shui-Qiao Yuan Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Laboratory of Animal Center Huazhong University of Science and TechnologyWuhan 430030China Shenzhen Huazhong University of Science and Technology Research Institute Shenzhen 518057China

Mammalian testis exhibits remarkably high transcriptome complexity,and spermatogenesis undergoes two periods of transcriptional *** make the RNA-binding proteins(RBPs)the utmost importance during male germ cell *** nuclear ribonucleoproteins(hnRNPs)are a large family of RBPs implicated in many steps of RNA processing;however,their roles in spermatogenesis are largely ***,we investigated the expression pattern of 12 hnRNP family members in mouse testes and found that most detected members are highly expressed in the ***,we found that most of the detected hnRNP proteins(hnRNPD,hnRNPK,hnRNPQ,hnRNPU,and hnRNPUL1)display the highest signals in the nuclei of pachytene spermatocytes,round spermatids,and Sertoli cells,whereas hnRNPE1 exclusively concentrates in the manchette of elongating *** expression of these hnRNP proteins showed both similarities and specificity,suggesting their diverse roles in spermatogenesis.

关键词： gene expression heterogeneous nuclear ribonucleoproteins mice Sertoli cells spermatogenesis

Differentiation of rat marrow mesenchymal stem cells into pancreatic islet beta-cells

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World Journal of Gastroenterology 2004年第20期10卷 3016-3020页

作者： li-BoChen xiao-BingJiang lianYang DepartmentofSurgery UnionHospitalofHuazhongUniversityofScienceandTechnologyWuhan430022HubeiProvinceChina

AIM: To explore the possibility of marrow mesenchymal stem cells (MSC) in vitro differentiating into functional isletlike cells and to test the diabetes therapeutic potency of Islet-like cells. METHODS: Rat MSCs were isolated from Wistar rats and cultured. Passaged MSCs were induced to differentiate into islet-like cells under following conditions: pre-induction with L-DMEM including 10 mmol/L nicotinamide+l mmol/L β-mercaptoethanol+200 mL/L fetal calf serum (FSC) for 24 h, followed by induction with serum free H-DMEM solution including 10 mmol/L nicotinamide+1 mmol/L,β-mercaptoethanol for 10 h. Differentiated cells were observed under inverse microscopy, insulin and nestin expressed in differentiated cells were detected with immunocytochemistry. Insulin excreted from differentiated cells was tested with radioimmunoassay. Rat diabetic models were made to test in vivo function of differentiated MSCs. RESULTS: Typical islet-like clustered cells were observed. Insulin mRNA and protein expressions were positive in differentiated cells, and nestin could be detected in predifferentiated cells. Insulin excreted from differentiated MSCs (446.93±102.28 IU/L) was much higher than that from pre-differentiated MSCs (2.45+0.81 IU/L (Plike functional cells can be differentiated from marrow mesenchymal stem cells, which may be a new procedure for clinical diabetes stem -cell therapy, these cells can control blood glucose level in diabetic rats. MSCs may play an important role in diabetes therapy by islet differentiation and transplantation.

关键词：老鼠骨髓间叶细胞细胞系统胰岛 β细胞免疫细胞化学

Study on the properties of solar cell under bias condition by using impedance spectroscopy

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Optoelectronics Letters 2024年第9期20卷 518-522页

作者： xiao Wenbo li Ao xiao Bangzhi Key Laboratory of Nondestructive Test Ministry of EducationNanchang Hangkong UniversityNanchang 330063China Science and Technology College Nanchang Hangkong UniversityGongqingcheng 332020China School of Engineering and Construction Nanchang UniversityNanchang 330031China

This paper investigates the properties of silicon cells(SI)and perovskite solar cells(PSC)under bias condition by using impedance *** parallel resistances R_(p) of SI and PSC are found to decrease with increasing bias,but the capacitance C_(p) shows the opposite *** Rp with C_(p),bias has a greater impact on the C_(p) of both cells.

关键词： spectroscopy impedance condition

Solvent atmosphere controlled self-assembly of unmodified C_(60):A facile approach for constructing various architectures

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Chinese Chemical Letters 2014年第6期25卷 912-914页

作者： Xuan Zhang Xu-dong li College of Chemistry Chemical Engineering & BiotechnologyDonghua University

A facile approach for constructing diverse architectures of unmodified C60 was developed via simple evaporation of pure C60 solution in CS2 under various poor solvent atmospheres. Diverse architectures such as belts, sheets, and starfishes were successfully constructed under different experimental conditions. C60 belts obtained under EtOH atmosphere were confirmed to be a face-centered cubic （fcc） structure. The solvent atmospheres not only slowed clown the evaporation speed, but also could reorganize the self-assembly of C60 by partially re-dissolving the initially formed architectures. This concept represents a novel method for preparation of nanostructures of C60 and could also be applied for controlling of the self-assembly of other functional organic molecules.

关键词： Fullerene Self-assembly Nanostructure Atmosphere Evaporation

A Wavelet and Neural Networks Based on Fault Diagnosis for HAGC System of Strip Rolling Mill

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Journal of Iron and Steel Research International 2011年第1期18卷 31-35页

作者： li Guo you dong Min Key Laboratory of InduslriaI Computer Control Engineering of Hebei Province Yanshan University Qinhuangdao 066004 Hebei China College of Mechanical Engineering Yanshan University Qinhuangdao 066004 Hebei China

The fault diagnosis of HAGC （Hydraulic Gauge Control） system of strip rolling mill is researched. Taking the advantage of the accompanying characteristics of the closed loop control system, rolling force forecasting model is built based on neural networks. The comparison results of the prediction and the actual signal are taken as residual signals. Wavelet transform is used to obtain the components of high and low frequency of the residual signal. Wave let decomposition results make fault feature clear and time-domain positioning accurately. Fault numerical criterion is established through lipschitz exponent. By analyzing the varied fault features which correspond to varied fault rea sons, the fault diagnosis of HAGC system is implemented successfully.

关键词： HAGC fault diagnosis neural network wavelet transform

Synthesis and characterization on a novel series of protic pyrrolidinium surfactants

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Chinese Chemical Letters 2010年第4期21卷 385-387页

作者： Ma, Fu Min Chen, xiao Wang, Xu dong Zhao, Yu Rong li, Qiu Hong Yue, Xiu Lv, Chao Shandong Univ Minist Educ Key Lab Colloid & Interface Chem Jinan 250100 Peoples R China

A novel series of protic tertiary pyrrolidinium surfactants were prepared and characterized by different *** compounds show good conductivity and efficient ability to reduce surface *** analysis proves their high thermal stability at decomposition temperatures over 250℃.Their lyotropic and thermotropic liquid crystalline properties are also discussed.

关键词： Protic pyrrolidinium surfactants liquid crystal Conductivity Surface tension