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Identification of a denitrifying bacterium and verification of its anaerobic ammonium oxidation ability

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Science China(life Sciences) 2006年第5期49卷 460-466页

作者： HU Baolan ZHENG Ping li Jinye XU xiangyang JIN Rencun Department of Environmental Engineering Zhejiang University Hangzhou 310029 China Department of Environmental Engineering Zhejiang University Hangzhou 310029 China Department of Environmental Engineering Zhejiang University Hangzhou 310029 China Department of Environmental Engineering Zhejiang University Hangzhou 310029 China Department of Environmental Engineering Zhejiang University Hangzhou 310029 China

A strain D3 of denitrifying bacterium was isolated from an anammox reactor,and identi-fied as Pseudomonas mendocina based on the morphological and physiological assay,Vitek test,Biolog test,(G+C) mol% content,and 16S rDNA phylogenetic *** a typical denitrifying bac-terium,strain D3 achieved the maximal nitrate reduction rate of 26.2 mg/(L·d) at the nitrate concen-tration of 88.5 mg N/*** optimal pH and growth temperature were 7.84 and 34.9℃,*** D3 was able to oxidize ammonia under anaerobic *** maximum nitrate and ammo-nium utilization rates were 6.37 mg/(L·d) and 3.34 mg/(L·d) ,respectively,and the consumption ratio of ammonia to nitrate was 1:*** microscopic observation revealed peculiar cell inclusions in strain *** of its relation to anammox activity,strain D3 was presumed to be *** present investigation proved that denitrifying bacteria have the anammox ability,and the results have engorged the range of anammox populations.

关键词： Pseudomonas mendocina, denitrifying bacteria, anammox bacteria, anammoxosome.

Data compensation based on the additional feature information for collaborative interactive operation with optical human motion capture system

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International Journal of Modeling, Simulation, and Scientific Computing 2018年第6期9卷 234-251页

作者： xiangyang li Rui Wang Zhe Xu Lei Pan Zhili Zhang Xi’an Research Institute of High Technology 710025 Xi’an P.R.China

As the effective capture region of optical motion capture system is limited by quantity,installation mode,resolution and focus of infrared cameras,the reflective markers on certain body parts(such as wrists,elbows,etc.)of multi-actual trainees may be obscured when they perform the collaborative interactive *** address this issue,motion data compensation method based on the additional feature information provided by the electromagnetic spatial position tracking equipment is proposed in this *** main working principle and detailed realization process of the proposed method are introduced step by step,and the practical implementation is presented to illustrate its validity and *** results show that the missing capture data and motion information of relevant obscured markers on arms can be retrieved with the proposed method,which can avoid the simulation motions of corresponding virtual operators being interrupted and deformed during the collaborative interactive operation process performed by multiactual trainees with optical human motion capture system in a limited capture range.

关键词： Data compensation additional feature information collaborative interactive operation human motion capture reflective markers.

Natural human–computer interaction control of multi operators in collaborative virtual maintenance based on optical human motion capture system

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International Journal of Modeling, Simulation, and Scientific Computing 2016年第2期7卷 48-62页

作者： xiangyang li Zhili Zhang Feng liang Qinhe Gao lilong Tan Xi’an Research Institute of Hi–Tech Xi’an People’s Republic of China

Aiming at the human–computer interaction control(HCIC)requirements of multi operators in collaborative virtual maintenance(CVM),real-time motion capture and simulation drive of multi operators with optical human motion capture system(HMCS)is *** detailed realization process of real-time motion capture and data drive for virtual operators in CVM environment is presented to actualize the natural and online interactive *** order to ensure the cooperative and orderly interactions of virtual operators with the input operations of actual operators,collaborative HCIC model is established according to specific planning,allocating and decision-making of different maintenance tasks as well as the human–computer interaction features and collaborative maintenance operation features among multi maintenance trainees in CVM ***,results of the experimental implementation validate the effectiveness and practicability of proposed methods,models,strategies and mechanisms.

关键词： Human-computer interaction collaborative virtual maintenance virtual reality human motion capture system concurrent conflict control mechanism.

DNA methylation changes induced by BDE-209 are related to DNA damage response and germ cell development in GC-2spd

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Journal of Environmental Sciences 2021年第11期33卷 161-170页

作者： xiangyang li Yue Zhang Xiaomin Dong Guiqing Zhou Yujian Sang Leqiang Gao Xianqing Zhou Zhiwei Sun Department of Toxicology and Hygienic Chemistry School of Public HealthCapital Medical UniversityBeijing 100069China Beijing Key Laboratory of Environmental Toxicology Capital Medical UniversityBeijing 100069China Experimental Center for basic medical teaching Basic Medical SciencesCapital Medical UniversityBeijing 100069China

Decabrominated diphenyl ether(BDE-209)is generally utilized in multiple polymer materials as common brominated flame ***-209 has been listed as persistent organic pollutants(POPs),which was considered to be reproductive toxin in the *** it still remains unclear about the effects of BDE-209 on DNA methylation and the inducedmale reproductive *** to the extensive epigenetic regulation in germ line development,we hypothesize that BDE-209 exposure impacts the statue of DNA methylation in spermatocytes in ***,the mouse GC-2spd(GC-2)cells were used for the genome wide DNA methylation analysis after treated with 32μg/mL BDE-209 for 24 *** results showed that BDE-209 caused genomic methylation changes with 32,083 differentially methylated CpGs in GC-2 cells,including 16,164(50.38%)hypermethylated and 15,919(49.62%)hypomethylated *** integrated analysis ofDNAmethylation data and functional enrichment,we found that BDE-209 might affect the functional transcription in cell growth and sperm development by differential gene ***-PCR validation demonstrated the involvement of p53-dependent DNA damage response in the GC-2 cells after BDE-209 *** general,our findings indicated that BDE-209-induced genome wide methylation changes could be interrelated with reproductive *** study might provide new insights into the mechanisms of male reproductive toxicity under the environmental exposure to BDE-209.

关键词： Decabrominated diphenyl ether (BDE-209) DNA methylation Functional enrichment DNA damage response Germ cell development GC-2spd

Exact elastic impedance tensor for isotropic media

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Science China Earth Sciences 2015年第6期58卷 1350-1360页

作者： ZHANG Feng li xiangyang State Key Laboratory of Petroleum Resource and Prospecting China University of Petroleum

The existing expressions of elastic impedance,as the generalized form of acoustic impedance,represent the resistance of subsurface media to seismic waves of non-normal incidence,and thus include information on the shear-wave *** this sense,conventional elastic impedance is an attribute of the seismic reflection and not an intrinsic physical property of the subsurface *** derivation of these expressions shares the approximations made for reflectivity,such as weak impedance contrast andisotropic or weakly anisotropic media,which limits the accuracy of reflectivity reconstruction and seismic *** this paper,we derive exact elastic impedance tensors of seismic P-and S-waves for isotropic media based on the stress-velocity *** componentof the impedance tensor represents a unique mechanical property of the *** of P-wave elastic impedance tensor components are discussed for seismic inversion and *** to synthetic data and real data shows the accuracy and robust interpretation capability of the derived elastic impedance in lithology characterizations.

关键词： stress tensor velocity tensor elastic impedance inversion reservoir characterization

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APPliCATION AND DEVELOPMENT OF ‘GREEN’ PREVENTIVE AND CONTROL TECHNOLOGIES IN GUIZHOU TEA PLANTATIONS

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Frontiers of Agricultural Science and Engineering 2022年第1期9卷 75-81页

作者： xiangyang li linhong JIN Zhuo CHEN Baoan SONG State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityGuiyang 550025China

The application and development of ‘green’ preventive technologies in teaplantations is an important means of ensuring tea quality and ecologicalsafety. Ecological, agronomic and biological controls are the main preventivemeasures used in Guizhou Province. This paper summarizes the ‘green’preventive technologies being applied in Guizhou tea plantations, including theuse of plant defense inducers to regulate tea plant responses to pathogens,natural enemies to control pest species causing damage to shoots andTrifolium repens to control the main weed species. In addition, it summarizesthe integrated ‘green’ preventive technologies being used in Guizhou andprovides a foundation for the ecological maintenance of tea plantations.

关键词： ‘green’prevention natural enemies plant defense inducers tea plantations Trifolium repens

Association-Dissociation of Glycolate Oxidase with Catalase in Rice： A Potential Switch to Modulate Intracellular H2O2 Levels

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Molecular Plant 2016年第5期9卷 737-748页

作者： Zhisheng Zhang Yuanyuan Xu Zongwang Xie xiangyang li Zheng-Hui He Xin-Xiang Peng State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural University Guangzhou 510642 China Department of Biotechnology College of Bioscience and Biotechnology Hunan Agricultural University Changsha 410128 China Department of Biology San Francisco State University San Francisco CA 94132 USA

Rapid and dynamic change in hydrogen peroxide （H2O2） levels can serve as an important signal to regulate various biological processes in plants. The change is realized by tilting the balance between its production and scavenging rates, in which membrane-associated NADPH oxidases are known to play a crucial role. Functioning independently from NADPH oxidases, glycolate oxidase （GLO） was recently demonstrated as an aitemative source for H2O2 production during both germ-for-germ and non-host resistance in plants. In this study, we show that GLO physically interacts with catalase （CAT） in rice leaves, and that the interaction can be deregulated by salicylic acid （SA）. Furthermore, the GLO-mediated H2O2 accumulation is synergistically enhanced by SA. Based on the well-known mechanism of substrate channeling in enzyme complexes, SA-induced H2O2 accumulation likely results from SA-induced GLO-CAT dissociation. In the GLO-CAT complex, GLO-mediated H2O2 production during photorespiration is very high, whereas the affinity of CAT for H2O2 （measured Km ≈ 43 raM） is extraordinarily low. This unique combination can further potentiate the increase in H2O2 when GLO is dissociated from CAT. Taken together, we propose that the physical association-dissociation of GLO and CAT, in response to environmental stress or stimuli, seems to serve as a specific mechanism to modulate H2O2 levels in rice.

关键词： association-dissociation catalase glycolate oxidase hydrogen peroxide salicylic acid

Growth and Transcriptomics Analysis ofMichelia macclurei Dandy Plantlets with Different LED Quality Treatments

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Phyton-International Journal of Experimental Botany 2023年第10期92卷 2891-2906页

作者： Zhaoli Chen Ying liu Bingshan Zeng Qingbin Jiang Shengkun Wang xiangyang li Research Institute of Tropical Forestry Chinese Academy of ForestryGuangzhou510520China

Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China,owing to its economic and practical *** quality influences plantlet growth and development during tissue ***,the growth characteristic and molecular regulation of *** under different light quality conditions are not well understood *** this study,we investigated the morphological,chlorophyll content,and transcriptomic responses of *** plantlets under different light-emitting diode(LED)qualities,including white,blue,and red *** results showed that blue light significantly increased plant height(21.29%)and leaf number(18.65%),while red light decreased plant height and leaf number by 7.53%and 16.49%,*** addition,the plantlets’chlorophyll content and etiolation rate were significantly reduced by blue and red light quality compared to white *** to white light,blue light had a negative effect,leading to decreased rooting rate(64.28%),root number(72.72%),and root length(75.86%).Conversely,red light had a positive effect,resulting in increased rooting rate(24.99%),root number(109.58%),and root length(72.72%).Transcriptome analysis identified 54 differentially expressed genes(DEGs)in three groups that consisted of blue light *** light(BL-vs-WL),red light *** light(RL-vs-WL),and red light *** light(RL-vs-BL).Specifically,21,7,and 41 DEGs were identified in the three groups,*** DEGs found in the RL-vs-WL and BL-vs-WL groups were involved in plant hormone signaling,nitrogen metabolism,and phenylpropanoid biosynthesis pathways,which suggests that *** plantlets adapt to the changes of light quality via modulating gene ***,our study provides valuable insights for understanding the molecular and morphological responses of *** plantlets under different light qualities.

关键词： Michelia macclurei plantlets growth characteristics transcriptome

Detection of gas reservoirs by the joint use of P- and PS-waves: A case study on the Ordos basin, China

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Earthquake Science 2009年第3期22卷 307-313页

作者： Xiucheng Wei xiangyang li Yang liu Songqun Shi Weidong Jiang Petroleum Exploration and Production Institute China Petroleum and Chemical Corporation Beijing 100083 China Edinburgh Anisotropy Project British Geological Survey Edinburgh EH9 3LA UK Faculty of Resources and Information China University of Petroleum Beijing 102249 China Changqing Research Institute China National Petroleum Corporation Xi'an 710021 China Changqing Institute of East Geophysical Company China National Petroleum Corporation Xi' an 710021 China

We present an example of using converted-waves for characterizing onshore gas reservoirs in the Ordos basin in Northwest China. The Ordos basin is the largest gas province in China. The main gas reservoirs （about 3 300 m in depth） are in upper Paleozoic sandstone that has low or reversed P-wave impedance and is immediately above a coal seam. This makes it very difficult to image the gas reservoirs using conventional P-wave data. Analysis of core, log and VSP data shows a weak PP reflection but a relatively strong PS-converted wave reflection, or both strong PP- and PS-reflections but with opposite polarity from the gas bearing sands, which indicates the potential of using PS-waves to image the gas reservoirs in the Ordos basin. Subsequently, thirteen seismic lines were acquired, processed and interpreted to verify the PP- and PS-responses, and two corresponding attributes （PP- and PS- amplitude ratio and polarity ratio） are used to map the reservoirs through joint PP and PS analysis.

关键词： gas reservoir P- and PS-waves well log data processing