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Synthesis, Crystal Structure, and Magnetic Properties of a Co Metal-organic Framework with Mixed Dicarboxylate and Tricarboxylate ligands

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Chinese Journal of Structural Chemistry 2016年第2期35卷 257-263页

作者： li wen-bin GAO Zhu-Qing GU Jin-Zhong School of Chemical and Biological Engineering Taiyuan University of Science and Technology College of Chemistry and Chemical Engineering Lanzhou University

One new mixed-ligand coordination polymer,namely[Co2（btc）（bpdc）（0.5）（py）3]n（1）,was hydrothermally synthesized using biphenyl-2,4,4＇-tricarboxylic acid（H3btc） as a main building block,along with two auxiliary ligands（H2bpdc = 2,2＇-bipyridine-5,5＇-dicarboxylic acid and py =pyridine）.The product 1 was characterized by IR spectroscopy,elemental,thermogravimetric,and single-crystal X-ray diffraction ***-crystal X-ray diffraction studies show that compound 1 possesses an intricate 3D metal-organic framework based on tetracobalt（Ⅱ） *** susceptibility measurement indicates that compound 1 shows an antiferromagnetic coupling between the Co（Ⅱ） ions.

关键词： metal-organic framework carboxylate ligand magnetic properties

Preparation and Microstructure of Cu/Ti_3SiC_2 Nanocomposite

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Chinese Journal of Aeronautics 2006年第B12期19卷 19-22页

作者： GU wan-li SHENG wen-bin CHEN Zong-min School of Mechanical Engineering Shandong University of Technology Zibo 255049 China

Mixed micron-sized Cu/Ti3SiC2 （vo15%） powder was mechanically milled using agate balls and zirconia balls separately. Then followed an examination of it with the FEI-SEM. The experimental results show that, distributed homogenously in Cu matrix, the Ti3SiC2 particles have a size of about 30-50 nm after milled with agate balls for 8 h, while it remains approximately unchanged after milled with zirconia balls. The microstructure of the mixture at different ball-milling stages was also studied. Bulks of Cu/Ti3SiC2 nano-composite were fabricated by hot pressing nano-sized Cu/Ti3SiC2 powder at the temperature of 1 073 K under 100 MPa. Then came an investigation of the effects of the particle size and agglomerate state of Ti3SiC2 as well as the microstructure of Cu/Ti3SiC2 nano-composite. It was found that the nano-sized Ti3SiC2 particles distribute evenly in copper.

关键词： nanocomposite Cu Ti3SiC2 powder metallurgy

A Modified Method for the Development of SSR Molecular Markers Based on Redundant EST Data and Its Application in Soybean

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Journal of Integrative Agriculture 2012年第4期11卷 545-555页

作者： ZHAO Xue CHANG Wei HAN Ying-peng TENG Wei-li li wen-bin Key Laboratory of Soybean Biology Ministry of Education/Soybean Research InstituteNortheast Agricultural UniversityHarbin 150030P.R.China

EST-derived SSR marker has been developed in many species,but few methods of high efficiency have been reported for the exploitation of EST-SSR ***,a high efficiency method for mining millions of redundant EST data is needed.A modified method for the EST-SSR development with high efficiency was established based on the redundant EST data of soybean in this *** method achieved its function through classifying ESTs according to the same SSR motif and detected candidate loci with redundant *** this study,a total of 80 polymorphic EST-SSR markers of soybean were developed,50 of them were exploited by this modified method which proved the higher speed and efficiency of this *** the 80 polymorphic EST-SSRs were mapped on soybean physical map through in silico mapping and 15 markers were integrated on a genetic map constructed in previous study.A software named hpSSR（high polymorphic SSR） was programmed based on the concept of the up-built method for EST-SSR *** method is not only pragmatic for EST-SSR exploitation in soybean,but also effective for the development of the marker in other species if the redundancy EST data is available.

关键词： EST-SSR soybean polymorphism genetic mapping in silico mapping

Relationships Between C_4 Enzyme Activities and Yield in Soybeans (Glycine max (L.) Merr.)

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Journal of Integrative Agriculture 2013年第3期12卷 406-413页

作者： HUANG Shan-shan li Chang-suo YANG Ming-liang li wen-bin WANG Ji-an Key Laboratory of Soybean Biology Ministry of Education/Soybean Research Institute Northeast Agricultural University Plant Protection Institute Heilongjiang Academy of Land Reclamation Sciences

To study the relationships between C4 enzyme activities and yield, C4 enzyme activities （phosphoenolpyruvate carboxylase （PEPCase）, NADP-malate dehydrogenase （NADP-MDH）, NADP-malic enzyme （NADP-ME）, and pyruvate phosphate dikinase （PPDK）） in different organs of ten soybean cultivars with different yields were measured at different growth stages in China. The result showed that four enzyme activities in C4 pathway were obviously different among cultivars, especially PPDK activity was not detected in the leaves of Dongnong 1567 and Dongnong 1068 and the young leaves of Gongjiao 9107-1 and Dongnong 97-172, but there were weak activities in pod coats. The order of C4 enzyme activities is young leaves 〈 old leaves 〈 pod coats. The correlation coefficients between PEPCase activity and yield and between NADP-MDH activity at blooming stage and yield were 0.6979 and 0.6565, respectively, and both reached the significant level （5%）, and PEPCase activity kept significant positive correlation with plant photosynthetic rate. There was a negative correlation between NADP-ME activity and yield, and no correlation was found between PPDK activity and yield.

关键词： soybean photosynthetic rate yield C4 enzyme

MicroRNA Primary Transcripts and Promoter Elements Analysis in Soybean (Glycine max L. Merril.)

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Journal of Integrative Agriculture 2013年第9期12卷 1522-1529页

作者： li Jing liU Yong-xin HAN Ying-peng li Yong-guang GUO Mao-zu li wen-bin Key Laboratory of Soybean Biology Ministry of Education/Northeast Agricultural University Department of Computer Science and Technology Harbin Institute of Technology

The importance of microRNA （miRNA） at the post-transcriptional regulation level has recently been recognized in both animals and plants. In recent years, many studies focused on miRNA target identification and functional analysis. However, little is known about the transcription and regulation of miRNAs themselves. In this study, the transcription start sites （TSSs） for 11 miRNA primary transcripts of soybean from 11 miRNA loci （of 50 loci tested） were cloned by a 5＂ rapid amplification of cDNA ends （5＂ RACE） procedure using total RNA from 30-d-old seedlings. The features consistent with a RNA polymerase II mechanism of transcription were found among these miRNA loci. A position weight matrix algorithm was used to identify conserved motifs in miRNA core promoter regions. A canonical TATA box motif was identified upstream of the major start site at 8 （76%） of the mapped miRNA loci. Several cis-acting elements were predicted in the 2 kb 5＂ to the TSSs. Potential spatial and temporal expression patterns of the miRNAs were found. The target genes for these miRNAs were also predicted and further elucidated for the potential function of the miRNAs. This research provides a molecular basis to explore regulatory mechanisms of miRNA expression, and a way to understand miRNA-mediated regulatory pathways and networks in soybean.

关键词： soybean miRNA primary transcript RACE TATA box motif promoter cis-acting element

Identification of QTLs Associated with Total Soyasaponin Content in Soybean (Glycine max (L.) Merr.)

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Journal of Integrative Agriculture 2012年第12期11卷 1976-1984页

作者： HUANG Shan-shan HAN Ying-peng li Chang-suo TIAN Jun li wen-bin WANG Ji-an Key Laboratory of Soybean Biology Ministry of Education/Soybean Research Institute Northeast Agricultural University Plant Protection Institute Heilongjiang Academy of Land Reclamation Sciences

Soyasaponins are valuable compounds in certain drugs, industry, food additives and surfactants. Selecting cultivars with higher-soyasaponin content along with agronomic traits is a main goal for many soybean breeders. The aim of the present study was to identify the quantitative trait loci （QTLs） associated with total soyasaponin content through a F2 population, which was derived from a cross between Ha 91016 （higher soyasaponin content cultivar, 16.8 mg gl） and N98-9445A （lower soyasaponin content, only 5.7 mg g-l）. A genetic linkage map including a total of 162 simple sequence repeat markers was constructed, which covered the total length 2 735.5 cM, and the average distance between markers was 16.96 cM. Two QTLs associated with total soyasaponin content were identified. One, qSAP1 （located in sat_044-satt102 of linkage group （LG） K）, could explain 12.6% of phenotypic variance. The other, qSAP_2, was located between satt368 and sat413 of LG Dla, which could explain 15.8% of phenotypic variance. It was concluded that the two QTLs would have some potential value for marker-assisted selection for high-soyasaponin content breeding in soybeans.

关键词： quantitative trait loci (QTL) simple sequence repeats (SSR) marker-assisted selection (MAS) soybean,soyasaponin

Assessment on Phosphorus Efficiency Characteristics of Soybean Genotypes in Phosphorus-Deficient Soils

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Agricultural Sciences in China 2008年第8期7卷 958-969页

作者： PAN Xiang-wen li wen-bin ZHANG Qiu-ying li Yan-hua liU Ming-shan Key Laboratory of Black Soil Ecology Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Harbin 150081 P.R. China Key Laboratory of Soybean Biology Ministry of Education/Northeast Agricultural University Harbin 150030 P.R.China Agricultural Center of Wanlong Town Government Shuangcheng 150119 P.R.China

A glasshouse study compared the growth and phosphorus （P） efficiency of 96 genotypes of soybean [Glycine max （L.） Merrill] in a P-deficient soil. The soybean genotypes differed greatly in growth, nodulation and P uptake after growing in the soil for 45 days, with shoot biomass ranging from 0.91 to 1.75 g per plant. The application of P improved biomass production, nodulation and P uptake and decreased root to shoot ratio, root length and surface area and P utilization efficiency. The 96 soybean genotypes were divided into 3 categories in P efficiency using the principal component analysis and cluster analysis, and 4 categories according to F values in combination with growth potentials. The Pefficient genotypes were associated with high biomass production, root to shoot ratio, root length and surface area and P uptake but low shoot to root P concentration ratio under P deficiency. The results indicate that there is a substantial genotypic variation in P efficiency in existing germplasm, and that P efficiency was correlated positively with dry weights of shoots and roots, ratio of root to shoot dry weight, root length and surface area, root P content and total P uptake. The shoot dry weight under P deficiency and relative shoot dry weight （deficient P/adequate P supply） are effective and simple indicators for screening P-efficient genotypes at the seedling stage.

关键词： assessment phosphorus efficiency characteristics phosphorus-deficient soil soybean genotypes

Nectar secretion of RN-type cytoplasmic male sterility three lines in soybean [Glycine max(L.) Merr.]

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Journal of Integrative Agriculture 2018年第5期17卷 1085-1092页

作者： ZHANG Jing-yong SUN Huan ZHAO li-mei ZHANG Chun-bao YAN Hao PENG Bao li wen-bin Soybean Institute Northeast Agricultural University/Key Laboratory of Soybean BiologyMinistry of Education/Key Laboratory of Soybean Biology and Breeding (Genetics)Ministry of Agriculture Soybean Research Institute Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean

Significant progress has been achieved in the use of heterosis in soybean and several soybean hybrids have been released in China. However, broad use of hybrid soybean seed is limited due to low seed setting of female parents. Breeding cytoplasmic male sterile（CMS） lines with high out-crossing rate is necessary to solve the problem. The objective of this study was to determine the relationship between out-crossing rate of CMS lines and their nectar secretion. The daily nectar secretion rhythm, meteorological effect on nectar secretion, and differences in nectar secretion among genotypes and years were investigated in 27 soybean CMS lines（A-lines） with their maintainers（B-lines） and restorers（R-lines）. The correlation between out-crossing rate of CMS lines and nectar production was also evaluated. Nectar secretion had diurnal variation. Secretion initiated at about 06：00 for most materials and reached a peak at 07：00–08：30 after flower opened, then the nectar secretion decreased gradually. A sub-peak appeared at about 13：00, while the nectar could not be detected at 17：00. Nectar secretion was greatly influenced by the weather conditions. The amount of nectar secretion increased gradually over time during periods of high temperature and no rainfall for several days. Rainy weather and low temperatures inhibited nectar secretion. There were obvious variations of nectar amount among different genotypes tested. Significant nectar variation within a genotype among years was also observed, and the highest nectar secretion was 3-fold higher than the lowest. The amount of nectar secretion from R-lines was significantly higher than that of A-and B-lines. There was no significant difference in nectar secretion between A-and B-lines. A-and B-lines with higher out-crossing rates secreted more nectar. The amount of nectar secretion of A-and B-lines were significantly positively correlated with the out-crossing rate of A-lines.

关键词： soybean RN cytoplasmic male sterility nectar secretion out-crossing rate

Infectivity Analysis of Cloned Genomic DNA of P1 Agent in vitro

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畜牧兽医学报 2009年第S1期40卷 43-46页

作者： wen li-bin HE Kong-wang YANG Han-chun Institute of Veterinary Medicine Jiangsu Academy of Agricultural Sciences College of Veterinary Medicine China Agricultural University

The ultrastructure of porcine kidney(PK)-15 cells was examined after lipofectamine-aided transfection of the molecular clone of the P1 ***-15 cells transfected with the tandem dimer of the P1molecular DNA clone had numbers of intracytoplasmic inclusions,and a few cells had intranuclear *** inclusions were round to oval and 0.1-0.3μm in diameter,and intranuclear inclusions,which were more electron dense,were of two general types:the first were round and small(0.1μm approximately)and the second were hexagonal and larger(0.4-0.8μm in diameter).Cells transfected with the tandem dimer of the P1 molecular DNA clone tested positive for P1 DNA at passage *** is the first report that the P1 molecular clone has infectivity in vitro and it will provide fundamental materials for further study of the biological characterization of P1.

关键词： P1 agent molecular clone in vitro infectivity analysis