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Pithovirus: a new giant DNA virus found from more than 30,000-year-old sample

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Virologica Sinica 2014年第2期29卷 71-73页

作者： Zheng Kou tianxian li State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences

A third type of giant DNA virus named Pithovirus was recently isolated from a ＞30,000-year-old permafrost sample in Siberia （Legendre M,et al,2014）.Pithovirus has a larger pandoravirus-like particle with 1.5 μm length and its adenine-thymine-rich genome is about 600 kb,which is surprisingly smaller than *** is very slight genetic relationship between Pithovirus and Pandoravirus and their replication cycles significantly *** groundbreaking discovery of this ancient virus expands our understanding of giant DNA viruses and raises the concern of pathogen reemergence from permafrost regions because of the change of climate or the activity of human.

关键词：病毒学临床诊断实验

Freshwater Cyanophages

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Virologica Sinica 2013年第5期28卷 253-259页

作者： Han Xia tianxian li Fei Deng Zhihong Hu State Key Laboratory of Virology Virus Resource and Bioinformatics CenterWuhan Institute of VirologyChinese Academy of Sciences

Cyanophages are double-stranded DNA viruses that infect cyanobacteria, and they can be found in both freshwater and marine environments. They have a complex pattem of host ranges and play important roles in controlling cyanobacteria population. Unlike marine cyanophages, for which there have been a number of recent investigations, very little attention has been paid to freshwater cyanophages. This review summarizes the taxonomy and morphology, host range, distribution, seasonal dynamics, and complete genomes of freshwater cyanophages, as well as diagnostic markers that can be used to identify them.

关键词： Cyanophage Freshwater Morphology Cyanobacteria

Molecular patterns of avian influenza A viruses

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Chinese Science Bulletin 2008年第13期53卷 2002-2007页

作者： KOU Zheng LEI FuMin WANG ShengYue ZHOU YanHong li tianxian Hubei Bioinformatics and Molecular Imaging Key Laboratory Department of Systems Biology Huazhong University of Science and Technology Wuhan 430074 China State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China Institute of Zoology Chinese Academy of Sciences Beijing 100080 China Chinese National Human Genome Center Chinese Academy of Sciences Shanghai 201203 China

Avian influenza A viruses could get across the species barrier and be fatal to humans. Highly patho-genic avian influenza H5N1 virus was an example. The mechanism of interspecies transmission is not clear as yet. In this research,the protein sequences of 237 influenza A viruses with different subtypes were transformed into pseudo-signals. The energy features were extracted by the method of wavelet packet decomposition and used for virus classification by the method of hierarchical clustering. The clustering results showed that five patterns existed in avian influenza A viruses,which associated with the phenotype of interspecies transmission,and that avian viruses with patterns C and E could across species barrier and those with patterns A,B and D might not have the abilities. The results could be used to construct an early warning system to predict the transmissibility of avian influenza A viruses to humans.

关键词：禽流感病毒 H5N1 子波分解聚类种间传输

Molecular characterization of avian influenza virus (H7N8) isolated from poultry in Central China in the mid-1980s

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Chinese Science Bulletin 2010年第16期55卷 1625-1630页

作者： YANG Jialin XIA Han ZHAO JiuRu HE XiaoBin PAN liMin TANG Shuang ZHANG Zhong KOU Zheng li tianxian State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

The molecular and pathogenic properties of avian influenza virus (A/duck/Hubei/216/1985/H7N8) isolated from Hubei Province of China in 1985 were *** hemagglutinin gene (HA) of Dk/Hb/216/85/H7N8 had the multiple amino acid se-quences (-PEIPKGRG-) at the connecting peptide between HA1 and HA2, which is considered to be a distinguishing molecular characteristic of low *** key sites of host markers among the genes (M, NP, NS, PA and PB2) of Dk/Hb/ 216/85/H7N8 were similar to those of H5N1 *** analysis showed that the genes (M, NS and PB2) of Dk/Hb/ 216/85/H7N8 clustered closely with those of Gs/Guangdong/1996/H5N1 (Gs/GD/96), implying that three ancient genes of Gs/ GD/96-like viruses had been circulating in Central China during the *** experimental infection, Dk/Hb/216/85/H7N8 was lowly pathogenic to chickens and mice, consistent with the molecular feature of HA gene.

关键词：禽流感病毒分子特性中国年代血凝素基因孤立中期家禽

Molecular characterization of H3N2 and H4N6 subtypes avian influenza viruses isolated from mallards in Poyang Lake,China in 2010

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Chinese Science Bulletin 2012年第27期57卷 3586-3594页

作者： ZHU Na ZHAO JiuRu li YongDong DING ChangQing XIA Han TANG Shuang ZHANG Zhong YU JingFeng CHEN Jing FAN ZhaoJun KOU Zheng li tianxian State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of SciencesWuhan 430071China Ningbo Municipal Center for Disease Control and Prevention Ningbo 315010China College of Biology Beijing Forestry University Beijing 100083China

Poyang Lake is the largest inland freshwater lake in China and contains many species of wild birds and *** conducted a survey of avian influenza viruses in nine semi-artificial waterfowl farms in Poyang Lake during January to March of *** of 1036 cloacal swabs collected,three H3N2 and one H4N6 influenza viruses were isolated from healthy *** the isolates were genetically and phylogenetically *** analysis of putative HA cleavage sites showed that all the four isolates possessed the molecular characteristics(QTRGL for H3N2 viruses,PEKASR for H4N6 virus) of lowly pathogenic avian influenza(LPAI) *** phylogenetic analysis of the viral genomes showed that all four virus isolates clustered in the Eurasian clade of influenza *** M gene of the viruses possessed the highest homology with highly pathogenic H5N1 influenza *** addition,co-infection of H3N2 and H4N6 in the same farm was *** interestingly,we isolated two subtypes viruses(H3N2 and H4N6) and their progeny virus(H3N2) with evidence of genome reassortment from the same farm,in which the PB1 and PB2 gene segments of H4N6 replaced those of the H3N2 *** results of animal infection experiments showed that all the four isolated viruses were lowly pathogenic to chickens and not pathogenic to mice,which was consistent with the results of genetic analysis.

关键词：禽流感病毒病毒分离分子特征鄱阳湖野鸭亚型中国系统发育分析

The limited number of available nucleotide and protein sequence data from the recent H7N9 cases in China impeded investigation and characterization of the outbreak

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Virologica Sinica 2014年第2期29卷 126-127页

作者： Haizhou liu Na Han Wei Fang James Adams Kou Zheng tianxian li Zhihong Hu Simon Rayner Key Laboratory of Agricultural and Environmental Microbiology and State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences

Dear Editor,Nucleotide and protein sequences of isolates collectedfrom infected populations can be useful for determiningthe threats,such as host adaptation,which are associatedwith the emergence of new *** 2013 saw thefirst reports of a new H7N9 lineage in Shanghai,Chinathat saw a gradual increase in the number of cases;but,by 17th May 2013,four Chinese provinces had

关键词：蛋白质序列序列数据核苷酸中国病例鉴定爆发上市

Study of the Dynamics of Microcystis aeruginosa and its Cyanophage in East Lake using quantitative PCR

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Virologica Sinica 2013年第5期28卷 309-311页

作者： Han Xia Meiniang Wang Xingyi Ge Yongquan Wu Xinglou Yang Yuji Zhang tianxian li Zhengli Shi Virus Resource and Bioinformatics Center State Key Laboratory of VirologyWuhan Institute of VirologyChinese Academy of Sciences Center for Emerging Infectious Diseases State Key Laboratory of VirologyWuhan Institute of VirologyChinese Academy of Sciences

Dear Editor,East Lake,located in the middle and lower reaches of the Yangtze River,is a major source of drinking water for local ***,the large increase of population density and the rapid development of industry has r... 详细信息

关键词：铜绿假单胞菌定量PCR 东湖噬藻体蓝藻水华长江中下游铜绿微囊藻人口密度

提高H13与3Cr2W8V压铸模寿命的措施

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特种铸造及有色合金 1998年第S1期18卷 17-20页

作者：王聚庆李天险中原机械厂

研究了3Cr2W8V与H13钢制造的铝合金压铸模的寿命对比关系,机械加工性能、耐热疲劳性能及热处理与寿命之间的关系。

关键词：加工性能耐热疲劳压铸模寿命

猪脾细胞干扰素的研制和提高母猪繁殖力的初步研究

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中国兽医杂志 2000年第7期26卷 7-9页

作者：陈绳亮李天宪肖作焕李绍珍中国科学院武汉病毒研究所武汉430071 湖北省农科院畜牧兽医研究所武汉430064

猪脾细胞干扰素 (PSIFN)即猪用增仔促孕素 ,是用猪脾脏细胞生产的 α-干扰素。本文简要介绍PSIFN的研制方法 ,其原料来源广、成本低 ,具有较高的推广价值。PSIFN能促使母猪在妊娠识别期处于良好状态 ,能提高母猪子宫对胚胎附植的承载能... 详细信息

猪脾细胞干扰素 (PSIFN)即猪用增仔促孕素 ,是用猪脾脏细胞生产的 α-干扰素。本文简要介绍PSIFN的研制方法 ,其原料来源广、成本低 ,具有较高的推广价值。PSIFN能促使母猪在妊娠识别期处于良好状态 ,能提高母猪子宫对胚胎附植的承载能力 ,改善母猪的繁殖性能 ,提高母猪的窝平均产仔数。这种以PSIFN配合

关键词：猪脾细胞干扰素研制母猪繁殖性能