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In vitro protective effect of recombinant prominin-1 combined with microRNA-29b on N-methyl-D-aspartateinduced excitotoxicity in retinal ganglion cells

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International Journal of Ophthalmology(English edition) 2023年第11期16卷 1746-1755页

作者： Jun-hua li Guan-shun Yu Yu-Da Wang Tian-Kun li Eye Hospital and School of Ophthalmology and Optometry Wenzhou Medical UniversityWenzhou 325027Zhejiang ProvinceChina Eye Hospital of Wenzhou Medical University Hangzhou Branch Hangzhou 310020Zhejiang ProvinceChina Tianjin Eye Hospital Tianjin 300020China

AIM:To determine the in vitro protective effect of recombinant prominin-1(Prominin-1)+microRNA-29b(P1M29)on N-methyl-D-aspartate(NMDA)-induced excitotoxicity in retinal ganglion cells(RGCs).METHODS:RGC-5 cells were cultured,and NMDAinduced excitotoxicity at the range of 100–800μmol/L was assessed using the MTT assay.NMDA(800μmol/L)was selected as the appropriate concentration for preparing the cell model.To evaluate the protective effect of P1M29 on the cell model,Prominin-1 was added at the concentration of 1–6 ng/mL for 48h,and the cell survival was investigated with/without microRNA-29b.After obtaining the appropriate concentration and time of P1M29 at 48h,real-time polymerase chain reaction(PCR)was utilized to detect the relative mRNA expression of vascular endothelial growth factor(VEGF)and transforming growth factor(TGF)-β2.Western blot detection was applied to measure the phosphorylation levels of protein kinase B(AKT)and extracellular regulated protein kinases(ERK)in RGC-5 cells after treatment with Prominin-1.Apoptosis study of the cell model was conducted by flow cytometry for estimating the anti-apoptotic effect of P1M29.Immunofluorescence analysis was used to analyze the expression levels of VEGF and TGF-β2.RESULTS:MTT cytotoxicity assays demonstrated that P1M29 group had significantly higher cell survival rate than Prominin-1 group(P<0.05).Real-time PCR data indicated that the expression levels of VEGF were significantly increased in both Prominin-1 and P1M29 groups compared NMDA and microRNA-29b group(P<0.05),while TGF-β2 were significantly decreased in both microRNA-29b and P1M29 groups compared NMDA and Prominin-1 group(P<0.05).Western blot results showed that both Prominin-1 and P1M29 groups significantly increased the phosphorylation levels of AKT and ERK compared to NMDA and microRNA-29b groups(P<0.05).Flow cytometry analysis revealed that P1M29 could prevent RGC-5 cell apoptosis in the early stage of apoptosis,while immunofluorescence results showed that P1M29 group had higher expression of VE

关键词： Prominin-1 microRNA-29b vascular endothelial growth factor transforming growth factor-β2

Microglial depletion impairs glial scar formation and aggravates inflammation partly by inhibiting STAT3 phosphorylation in astrocytes after spinal cord injury

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Neural Regeneration Research 2023年第6期18卷 1325-1331页

作者： Zhi-Lai Zhou huan Xie Xiao-Bo Tian hua-li Xu Wei li shun Yao Hui Zhang The Spine Surgery Department Guangdong Second Provincial General HospitalGuangzhouGuangdong ProvinceChina The Second School of Clinical Medicine Southern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Anesthesiology ZhuJiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

Astrocytes and microglia play an orchestrated role following spinal cord injury;however,the molecular mechanisms through which microglia regulate astrocytes after spinal cord injury are not yet fully understood.Herein,microglia were pharmacologically depleted and the effects on the astrocytic response were examined.We further explored the potential mechanisms involving the signal transducers and activators of transcription 3(STAT3)pathway.For in vivo experiments,we constructed a contusion spinal cord injury model in C57BL/6 mice.To deplete microglia,all mice were treated with colony-stimulating factor 1 receptor inhibitor PLX3397,starting 2 weeks prior to surgery until they were sacrificed.Cell proliferation was examined by 5-ethynyl-2-deoxyuridine(EdU)and three pivotal inflammatory cytokines were detected by a specific Bio-Plex Pro^(TM) Reagent Kit.Locomotor function,neuroinflammation,astrocyte activation and phosphorylated STAT3(pSTAT3,a maker of activation of STAT3 signaling)levels were determined.For in vitro experiments,a microglia and astrocyte coculture system was established,and the small molecule STA21,which blocks STAT3 activation,was applied to investigate whether STAT3 signaling is involved in mediating astrocyte proliferation induced by microglia.PLX3397 administration disrupted glial scar formation,increased inflammatory spillover,induced diffuse tissue damage and impaired functional recovery after spinal cord injury.Microglial depletion markedly reduced EdU+proliferating cells,especially proliferating astrocytes at 7 days after spinal cord injury.RNA sequencing analysis showed that the JAK/STAT3 pathway was downregulated in mice treated with PLX3397.Double immunofluorescence staining confirmed that PLX3397 significantly decreased STAT3 expression in astrocytes.Importantly,in vitro coculture of astrocytes and microglia showed that microglia-induced astrocyte proliferation was abolished by STA21 administration.These findings suggest that microglial depletion impaired astrocyte proliferation and astro

关键词： astrocytes coculture colony-stimulating factor 1 receptor inhibitor EdU glia scar inflammatory response microglia phosphorylation proliferation spinal cord injury STAT3

Real-time monitoring of raster temperature distribution and width anomalies in fused filament fabrication process

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Advances in Manufacturing 2022年第4期10卷 571-582页

作者： Feng li Zhong-hua Yu Hao li Zhen-Sheng Yang Qing-shun Kong Jie Tang The State Key Laboratory of Fluid Power Transmission and Control College of Mechanical EngineeringZhejiang UniversityHangzhou310027People’s Republic of China

The aim of this study is to monitor the raster temperature distribution and width anomalies in a fused filament fabrication(FFF)process by an infrared(IR)array sensor.To achieve this goal,two experiments were conducted on a desktop FFF machine.For the first experiment,three normal samples with different raster widths were fabricated,and thermal images of the newly deposited rasters were collected during the process.To process the low-resolution images,a segmentation-based image processing method was proposed.The temperature distributions along the horizontal direction of the raster section and along the raster length were obtained.The temperature features that could indicate the raster widths were extracted and then fed to recognition models for training and testing.The classification performance of the models were evaluated based on the F-score.The models with high F1-scores could be used to recognise width anomalies online.For the second experiment,an abnormal sample with raster width anomalies was fabricated.The temperature features were extracted from the collected experimental data.The obtained features were then fed to the built and evaluated models to recognise the width anomalies online.The effectiveness of the monitoring method was validated by comparing the recognition results with the actual optical images.The support vector machine(SVM)and k-nearest neighbour(KNN)were adopted to build the recognition models.The F1-score and online recognition results of the models were compared.The comparison study shows that SVM is more suitable for our situation than KNN.A method for monitoring the temperature distribution and width anomalies of the FFF raster is provided in this paper.To the best of the authors’knowledge,this is the first study to explore the actual temperature distribution along the horizontal direction of the raster section,and the first study to monitor the width anomalies of the raster in the FFF process.

关键词： Fused filament fabrication(FFF) Process monitoring Infrared Temperature distribution Width anomalies

Effect of Indirect Transformation of Retained Austenite During Tempering on the Charpy Impact Toughness of a Low-Alloy Cr-Mo-V Steel

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Acta Metallurgica Sinica(English Letters) 2020年第10期33卷 1346-1358页

作者： Yong-Han li Zhong-hua Jiang Zhen-Dan Yang Jue-shun Zhu Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of Sciences72 Wenhua RoadShenyang 110016China

A modified tempering treatment has been designed in order to avoid the direct transformation of retained austenite(Ar)during tempering of a low-alloy Cr-Mo-V steel.Instead of the direct transformation of Ar into ferrite and M23C6 carbides during conventional tempering at 700℃,transformation into aggregate of ferrite and cementite has been forced by a pre-tempering at 455℃ before conventional tempering.Experiments have been performed on specimens quenched with cooling rates 1.5,3 and 12℃/s,providing different types of Ar within the as-quenched microstructures.The results show that the tempering modification does not improve the Charpy impact toughness at the highest quenching rate of 12℃/s,where the specimens cannot incur cleavage cracking induced from fine and discontinuous M23C6 carbides along lath interfaces.For the lowest quenching rate 1.5℃/s,the Charpy impact toughness can be improved,and the failure is dominated by carbide aggregates,which originate from the decomposed products of blocky Ar.This is because the tempering modification effectively suppresses the formation of coarse M23C6 carbides at interfaces between the carbide aggregate and bainitic matrix,thereby resulting in a relatively homogeneous distribution of M23C6 carbides inside carbide aggregates.Therefore,the tempering modification is recommended for large-scale forgings,in which relatively high quenching rates are difficult to achieve.

关键词： Bainite steel Retained austenite Impact toughness

Stress Wave Propagation Analysis on Vortex-Induced Vibration of Marine Risers

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China Ocean Engineering 2017年第1期31卷 30-36页

作者： li hua-jun WANG Chao liU Fu-shun HU Sau-Lon James Shandong Provincial Key Lab of Ocean Engineering Ocean University of China Department of Ocean Engineering University of Rhode Island

To analyze the stress wave propagation associated with the vortex-induced vibration（VIV） of a marine riser, this paper employed a multi-signal complex exponential method. This method is an extension of the classical Prony＇s method which decomposes a complicated signal into a number of complex exponential components. Because the proposed method processes multiple signals simultaneously, it can estimate the “global” dominating frequencies（poles） shared by those signals.The complex amplitude（residues） corresponding to the estimated frequencies for those signals is also obtained in the process. As the signals were collected at different locations along the axial direction of a marine riser, the phenomena of the stress wave propagation could be analyzed through the obtained residues of those signals. The Norwegian Deepwater Program（NDP） high mode test data were utilized in the numerical studies, including data sets in both the in-line（IL） and cross-flow（CF） directions. It was found that the most dominant component in the IL direction has its stress wave propagation along the riser being dominated by a standing wave, while that in the CF direction dominated by a traveling wave.

关键词： Vortex exponential utilized reconstructed riser directions interpolation dominated residues processed

Design, synthesis and immunomodulating activity of C-pseudonucleosides containing thiazolidin-4-one and phenyl connected by acetamide bond

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Chinese Chemical Letters 2016年第6期27卷 938-942页

作者： hua Chen shun-Kai Xing Fang Gao Na li Xiao-liu li Ming Meng Key Laboratory of Chemical Biology of Hebei Province College of Chemistry and Environmental Science Hebei University Medical School Hebei University

Several novel C-pseudonucleosides containing thiazolidin-4-one and phenyl connected by acetamide bond were rationally designed and easily synthesized at room temperature by using the unprotected sugar aldehyde as the starting material. The effects of the compounds on Con A-induced T cell proliferation were evaluated at five concentrations of 5, 10, 25, 50, and 100 mmol/L Interestingly,compounds 7a and 8a（n = 2, R = H） exhibited immunostimulating activities, while compounds 5a, 6a（n = 1, R = H） and 7b, 8b（n = 2, R = CH3） showed immunosuppressive activities. Another two compounds 5 b and 6b（n = 1, R = CH3） had no immunomodulating activities. These initial biological results suggested that subtle structural changes to the phenyl and acetamide bond of C-pseudonucleosides could have a significant effect on T cell proliferation bias, although it was difficult to formulate a rigorous structureactivity relationship based on the observed activities.

关键词： Immunomodulator C-Pseudonucleosides Thiazolidin-4-one Acetamide bond SAR analysis

Reduced SARS-CoV-2 infection risk is associated with the use of Seven-Flavor Herb Tea:A multi-center observational study in Shanghai,China

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Journal of Integrative Medicine 2023年第4期21卷 369-376页

作者： shun-xian Zhang Xiao-xu Chen Yong Zheng Bing-hua Cai Wei Shi Ming Ru Hui li Dan-dan Zhang Yu Tian Yue-lai Chen Clinical Research Center Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Medical Affairs Department Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Medical Affairs Department of Minhang District Health Committee Shanghai 201199China Medical Affairs Department of Fengxian District Health Committee Shanghai 201499China Medical Affairs Department of Jinshan District Health Committee Shanghai 200540China Medical Affairs Department of Xuhui District Health Committee Shanghai 200030China Medical Affairs Department of Changning District Health Committee Shanghai 200050China Medical Affairs Department Jinshan TCM-Integrated HospitalShanghai 201501China Sleep Medicine Center Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China

Objective:Omicron,a severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variant,is responsible for numerous infections in China.This study investigates the association between the use of Seven-Flavor Herb Tea(SFHT)and the risk of SARS-CoV-2 infection to develop precise and differentiated strategies for control of the coronavirus disease 2019(COVID-19).Methods:This case-control study was conducted at shelter hospitals and quarantine hotels in China.A total of 5348 laboratory-confirmed COVID-19 patients were enrolled between April 1 and May 31,2022,while 2190 uninfected individuals served as healthy controls.Structured questionnaires were used to collect data on demographics,underlying diseases,vaccination status,and use of SFHT.Patients were propensity-score-matched using 1:1 nearest-neighbor matching of the logit of the propensity score.Subsequently,a conditional logistic regression model was used for data analysis.Results:Overall,7538 eligible subjects were recruited,with an average age of[45.54±16.94]years.The age of COVID-19 patients was significantly higher than that of uninfected individuals([48.25±17.48]years vs[38.92±13.41]years;t=22.437,Plinical trial are warranted to confirm the finding.

关键词： COVID-19 SARS-CoV-2 Seven-Flavor Herb Tea

Hydrogels of Chemically Cross-linked and Organ-metallic Complexed Interpenetrating PEG Networks

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Chinese Journal of Polymer Science 2016年第5期34卷 637-648页

作者： Meng-jiao Dong shun-li liu lin-hua Tan 岑莲付国东 School of Chemistry and Chemical Engineering Southeast UniversityJiangning DistrictNanjing 211189China Shanghai Key Laboratory of Multiphase Materials Chemical Engineering Department of Product EngineeringSchool of Chemical EngineeringEast China University of Science and TechnologyShanghai 200237China National Tissue Engineering Center of China Shanghai 200241China

The aim of the present work was to prepare a well-defined hydrogel of chemically cross-linked and organ-metallic complexed interpenetrating PEG networks. The hydrogel was synthesized via the reaction of copper（I）- catalyzed 1,3-dipolar azide-alkyne cycloaddition（CuA AC） with poly（ethylene glycol）-dopamine（PEG-DA）（“Click Chemistry”） followed by complexation with Fe-（3＋） ions to crosslink the polymeric network. The chemical composition and morphology of the resulting hydrogels were characterized by Fourier transform infrared spectroscopy（FTIR）, -1H-NMR and scanning electron microscopy（SEM）. Swelling ratio, mechanical strength, conductivity, and degradation behaviors of the hydrogels were also studied. The effect of the polymer chain length on properties of hydrogels was explored. The compressive strength of hydrogels could reach as high as 13.1 MPa with a conductivity of 2.2 × 10^-5 S·cm^-1. The hydrogels also exhibited excellent thermal stability even at a temperature of 300 °C, whereas degradation of the hydrogel after 7 weeks was observed under a physiological condition. In addition, the hydrogel exhibited a good biocompatibility based on its in vivo performance through an in vivo subcutaneous implantation model. No inflammation and no obvious abnormality of the surrounding tissue were observed when the hydrogel was subcutaneously implanted for 2 weeks. This work is a step towards creating a new pathway to synthesize hydrogels of interpenetrating networks which could be of important applications in the future research.

关键词： hydrogel metallic implanted subcutaneous implantation compressive azide chemically polymeric glycol

Three-dimensional assessment of movement patterns of Sichuan snub-nosed monkeys affected by habitat structure in temperate forests

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Zoological Research 2023年第2期44卷 361-365页

作者： Hai-Tao Yang shun li Rong Hou Wen-Tao Song Yan-Wen Fu Yong-Bo li Xiao-Wei Wang Gang He Colin A.Chapman Qing-hua Guo Bao-Guo li Shaanxi Key Laboratory for Animal Conservation College of Life SciencesNorthwest UniversityXi’anShaanxi 710069China Institute of Remote Sensing and Geographic Information System School of Earth and Space SciencesPeking UniversityBeijing 100871China Ministry of Education Key Laboratory of Poyang Lake Wetland and Watershed Research College of Geography and EnvironmentJiangxi Normal UniversityNanchangJiangxi 330022China College of life Sciences Beijing Normal UniversityBeijing 100875China Shaanxi Key Laboratory for Animal Conservation Shaanxi Institute of ZoologyShaanxi Academy of SciencesXi’anShaanxi 710032China Biology Department Vancouver Island UniversityBritish ColumbiaV5K0A1 Canada School of life Sciences University of KwaZulu-NatalPietermaritzburg 3200South Africa

DEAR EDITOR,Movement patterns can reflect species-specific characteristics of individuals and animal groups at a given scale. Accurate three-dimensional(3D) assessment can quantify the relationship between movement patterns of an animal and its unique habitat. Here, we evaluated the effects of habitat structure on movement patterns of the golden snub-nosed monkey(Rhinopithecus roxellana). We used airborne light detection and ranging(liDAR) to map the 3D structure of the temperate forest in the Qinling Mountains(Shaanxi, China).

关键词： habitat movement temperate