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Preparation and Characterization of PD liA/Chondroitin Sulfate/Chitosan Seaffold for Peripheral Nerve Regeneration

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Journal of Wuhan University of Technology(Materials Science) 2008年第2期23卷 230-233页

作者： XU Haixing YAN Yuhua WAN Tao li shipu Biomedical Materials and Engineering Research Center Wuhan University of Technology Wuhan 430070 China Department of Pharmaceutical Engineering School of Chemical Engineering Wuhan University of Technology Wuhan 430070 China

A novel bioactive and bioresorbable PDLLA/chondroitin sulfate/chitosan scaffold was prepared via layer-by-layer（LBL） electrostatic-self-assembly （ESA） and the thermally induced phase separation （TIPS） technique. Chondroitin sulfate and chitosan were alternately deposited on the activated PDLLA substrate. The deposition process was monitored by UV-Vis absorbance spectroscopy. After frozen and lyophilized, the scaffold was characterized by attenuated total reflection （ATR）-FT-IR, XPS, SEM and AFM. The results showed that the scaffold was modified uniformly with a dense inner layer with few detectable pores and a porous sponge outer layer with the pore size about 5 μm, there was an obvious across section and the average thickness of each layer was about 9.4 nm.

关键词： PDLLA chondroitin sulfate chitosan peripheral nerve

In vitro Biological Evaluation of Graphene on Neuronal Cells

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Journal of Wuhan University of Technology(Materials Science) 2016年第4期31卷 925-930页

作者：易继凌赵政 li shipu YIN Yixia WANG Xinyu State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 China Biomedical Materials and Engineering Research Center of Hubei Province Wuhan University of Technology Wuhan 430070 China

We prepared graphene（GE） with a mean size of 3087 *** transition of graphene oxide（GO） to GE was confirmed by UV-visible spectroscopy,Fourier transform infrared spectroscopy（FTIR） and X-ray diffraction（XRD）.The experimental results of optical microscopic observation indicated that the GE ranged from 5 to 20 μg/mL did not affect the cell morphologies of the PC12 *** results of cell viability and membrane integrity assay supported that of optical microscopic observation and demonstrated that the GE ranged from 5 to 20 μg/mL presented no obvious ***,reactive oxygen species（ROS） assay suggested that an elevation of ROS level could be detected when the GE ranged from 20 to 100 μg/*** results showed that the GE ranged from 5 to 10 μg/mL presented an excellent in vitro biocompatibility and was one kind of potential biomaterials for neural tissue engineering.

关键词： ranged visible microscopic integrity suspensions elevation agglomeration purity cytotoxicity sputtering

Degradable PRGD/PDLLA/β-TCP/NGF composites promote differentiation and regulate gene expression in rat pheochromocytoma cells

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Chinese Science Bulletin 2013年第24期58卷 2979-2983页

作者： QIU Tong YU Chang YAN QiongJiao li shipu Biomedical Materials and Engineering Center Wuhan University of Technology State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology

In this study, we synthesized degradable PRGD/PDLLA/β-TCP/NGF composites to facilitate neuronal repair. To this end, we (1) examined the release of nerve growth factor (NGF) from the composites, (2) evaluated the differentiation status of the cells and (3) address how transcriptional activity may regulate the differentiation mechanism of these cells. NGF content was determined using enzyme-linked immunosorbent assay, while the cellular mRNA expression was examined by real-time PCR analysis. Our results indicated that NGF release was robust during the first 10 days and then stabilized at a lower level thereafter. Treatment of PC12 cells with the extract of the NGF-embedded composites induced the formation of neurites and, in some cases, net-like neurites. Analysis of the expression level of differentiation-related genes, such as TrkA, VGF, Rab1, GAP43 and β-tubulin Ⅱ, were significantly up-regulated. These findings suggest that these composites might be a suitable delivery system for growth factors like NGF that can be used to facilitate neuronal repair after injury.

关键词：细胞分化复合材料 PDLLA 基因表达合成降解嗜铬细胞瘤神经生长因子神经元损伤

Effect of Hydroxyapatitc Nanoparticles on K562 Cells in vitro

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Journal of Wuhan University of Technology(Materials Science) 2008年第2期23卷 222-224页

作者： CHEN Pei DAI Honglian HAN Yingchao YIN Meizhen li shipu Biomedical Materials and Engineering Center Wuhan University of Technology Wuhan 430070 China Nursing school Wuhan Railway Vocational College of Technology Wuhan 430205 China

Stable and single-dispersed hydroxyapatite （HAP） nanoparticles were synthesized with ultrasonic-assisted method. HAP nanoparticles were characterized by dynamic light scattering, XRD （X-ray diffraction） and TEM （Transmission Electron Microscopy）. The effect of HAP nanoparticles on the K562 human myelogenous leukemia cell line was investigated by MTT assay and cell count test, and the mechanism was studied through the changes of cell cycle and ultrastructure. The results showed that HAP nanoparticles inhibited the proliferation of K562 cells dramatically in vitro. HAP nanoparticles entered the cytoplasm of K562 cells and the cells were arrested at G/M phase, thus, the cells died directly.

关键词： hydroxyapatite(HAP) nanoparticles K562 cells MTT assay cell cycle

Preparation of Nano-TiO_2 by liquid Hydrolysis and Characterization of Its Antibacterial Activity

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Journal of Wuhan University of Technology(Materials Science) 2014年第2期29卷 407-409页

作者：张启焕 YAN Xin 邵荣 DAI Honglian li shipu College of Chemical and Biological Engineering Yancheng Institute of Technology The Biomedical Materials and Engineering Research Center Wuhan University of Technology

The nano-TiO_2 particles were prepared by liquid hydrolysis method and characterized using XRD. Its antibacterial activity against two representative bacterial, Escherichia eoli and Staphylococcus aureus, was also studied. The experimental results showed that the nano-TiO_2 caleinated at 600-700℃ contained the obvious anatase phase and exerted exeeUent antibacterial activity. The feature of antibacterial activity of nano- TiO_2 was non-strains specificity and exerted best antibacterial activity at concentration of 0.8 g/L.

关键词： liquid hydrolysis method Nano-TiO_2 antibacterial activity

Preparation and Drug-release Behavior of β-TCP Ceramics Drug Carrier in vitro

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Journal of Wuhan University of Technology(Materials Science) 2012年第6期27卷 1058-1060页

作者：张启焕 YAN Xin YAN Yuhua DAI Honglian JIANG Xin li shipu College of Chemical and Biological Engineering Yancheng Institute of Technology Biomedical Materials and Engineering Center Wuhan University of TechnologyWuhan 430070China

β-TCP ceramics drug carrier was first prepared and characterized. SEM showed that β-TCP carrier was in porous amorphous structure with diameters around 10 μm. The physical properties including apparent porosity, volume-weight, tensile strength and the permeability were measured and the results indicated those properties fit the clinical usage of β-TCP drug carrier. Furthermore, drug release experiment in vitro showed that the carrier could prolong drug release in simulated body fluid which provides basis for the clinical use of β-TCP ceramics as drug carrier.

关键词： β-TCP ceramics drug carrier physicochemical properties drug release clinical use

Effects of β-TCP Ceramics on Osteoblast Cellular Proliferating,Mineralization and Osteocalcin Expression

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Journal of Wuhan University of Technology(Materials Science) 2012年第1期27卷 107-109页

作者： QI Zhitao ZHANG Qihuan ZHENG Qiang DAI Honglian WANG Zisheng QIU Ming li shipu School of Chemical and Biological Engineering Yancheng Institute of Technology The Bio-material Research and Engineering Center Wuhan University of Technology Yancheng Tianbang Feed Technology Co.Ltd

After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.

关键词： β-TCP ceramics osteoblast cellular proliferating mineralization osteocalcin

Effects of the Interaction between Hydroxyapatite Nanoparticles and Hepatoma Cells

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Journal of Wuhan University of Technology(Materials Science) 2014年第3期29卷 635-642页

作者：尹美珍 XU Weiguo CUI Bingcun 戴红莲 HAN Yingchao YIN Yixia li shipu School of Medicine Hubei Polytechnic University Tongji Hospital Tongji Medical College Huazhong University of Science and Technology State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Biomedical Materials and Engineering Center Wuhan University of Technology

To gain a better understanding of the anticancer effects of hydroxyapatite （HAP） nanoparticles in vivo and in vitro, the effects of the interaction of HAP nanoparticles with hepatoma cells were explored. HAP nanoparticles were prepared by homogeneous precipitation and characterized by laser particle analysis and transmission electron microscopy （TEM）. HAP nanoparticles were observed to be uniformly distributed, with rod-like shapes and diameters in the range of 42.1-87.1 nm. Overnight attached, suspended, and proliferating Bel-7402 cells were incubated with HAP nanoparticles. Inverted microscopy observation revealed that HAP nanoparticles with a cell membrane showed good adsorption. TEM demonstrated that HAP nanoparticles were present on the surface of cells, continuously taken up by cells through endocytosis, and transported in vesicles close to the nucleus. Fluorescence microscopy showed that the concentrations of intracellular Ca2＋ labeled with Fluo-3 calcium fluorescent probe were significantly enhanced. In addition, inverted microscopy observation revealed that suspended cells treated with HAP nanoparticles did not adhere to the culture bottle, resulting in cell death. After the overnight attached cells were treated with HAP nanoparticles for 96 h with increasing doses of HAP nanoparticles, inverted microscopy observation revealed that cell proliferation was slowed and ceU-ceU adhesion was weakened. Feulgen staining and image analysis indicated that the nuclear DNA content of the cells was markedly reduced, and argyrophilic nucleolar organizer region （AgNOR） staining and image analysis indicated that the number of AgNORs was significantly decreased. Therefore, hepatoma cells brought about the adsorption, uptake, transport and degradation of HAP nanoparticles. In addition, HAP nanoparticles affected hepatoma cells with regard to cell-cell adhesion, cell and extracellular matrix adhesion, and DNA and protein synthesis; thus inhibiting cell proliferation. This und

关键词： hydroxyapatite nanoparticles hepatoma cells interaction effects cell adhesion

RGD Gifted PDLLA-PRGD Conduits Promotes the Sciatic Nerve Regeneration

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Journal of Wuhan University of Technology(Materials Science) 2014年第3期29卷 620-625页

作者：方晓青邱彤 XIE lijuan YIN Yixia li Binbin YAN Qiongjiao DAI Honglian WANG Xinyu li shipu Department of Paediatrics Wuhan General Hospital of Guangzhou Military Biomedical Materials and Engineering Center Wuhan University of Technology State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology

Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA films with RGD in vivo. The results revealed that RGD coating with the concentration of 100-500 ug/mL promoted the cell proliferation and boosted the cell migration. Molecularly, RGD coating also enhanced the expression of the proliferation related genes （c-fos and c-jun） and the cell behavior related genes （actin, tublin, tau and MAP1） at first stages of the seeding, which is similar to the effects from laminin coating. In vivo, RGD addition improved the recovery efficiency of the transected nerve in regard of the more survived Schwann cells in vivo and the formation of more mature myelin sheath. Taken together, RGD peptides are good candidates to enhance the biocompatibility of the biomaterials and facilitate the peripheral nerve regeneration by prompting responses in Schwann ceils.

关键词： ROD Schwann cells cell behavior PDLLA-PRGD conduit sciatic nerve regeneration