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Production of porcine cloned transgenic embryos expressing green fluorescent protein by somatic cell nuclear transfer

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Science china({1.fe Sciences) 2006年第2期49卷 164-171页

作者： ZHANG Yunhai1, PAN Dengke1,2. SUN Xiuzhu1, SUN Guojie1, WANG Xiaobo1, liU Xiaohui1, li Yan1, DAI Yunping1 & li ning1 1. state key {2. for agrobiotechnology, china agricultural university, Beijing 100094, china 2. College of Animal Science and Technology, china agricultural university, Beijing 100094, china state {1. {2. for {2. China Agricultural University Beijing 100094 China College of Animal Science Technology China Agricultural University Beijing 100094 China

In the present study, nuclear transferred embryos (NTEs) were reconstructed by using pig fetal fibroblasts as donors; in vitro matured oocytes as recipients. The effects of G41. selection on donor cells, duration of IVM of prepubertal gilt oocytes; oxygen tension in IVM of oocytes were investigated. The results were as follows: (i) When G41. selected cells expressing GFP were used as donors, the cleavage rate of NTEs decreased drastically in comparison to NTEs derived from donors without antibiotic selection (47.5% vs. 71.6%, p>0.05). For the blastocyst rate, no significant difference was observed between two groups (1.% vs. 1..4%, pduration time was extended from 36 to 42.h (83.6% vs. 96.7%, p>0.05). However, no statistical difference was observed between NTEs derived from oocytes of 36 h IVM group; NTEs from oocytes of 42.h IVM group in the rates of cleavage (59.3% vs. 73.6%, p1..2., p2. tension; NTEs derived from oocytes matured under higher oxygen tension (2.% O2. in cleavage rate (70.6% vs. 67.1., p1..8% vs. 1.2}3%, p1. selection does not have a significant effect on cleavage rate of NTEs expressing GFP. (ii) Nuclear maturation is greatly improved by prolonging IVM duration from 36 to 42.h, while no significant differences were observed for developmental potential of transgenic embryos. Thus IVM 42.h is the better choice in order to obtain maximum number of MII oocytes as recipients. (iii) Lower oxygen tension; higher oxygen tension in IVM have no significant effect on development of cloned embryos.

关键词： transgenic cloning, GFP, Pig, early development.

Genetic analysis of segregation distortion of mating-type factors in Lentinula edodes

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Progress in Natural Science:Materials International 2005年第8期15卷 684-688页

作者： CHENG Shuiming 1,2. liN Fanxue1, XU Xuefeng1, li Anzheng1 and liN Fangcan1*(1. state key laboratory of {2. Microbiology and Institute of Applied Mycology, Huazhong {2. university, Wuhan 430070, china 2. National Engineering Research Center For Wheat, Henan {2. university, Zhengzhou 450002. china) state {1. {2. of {2. Microbiology and Institute of Applied Mycology Huazhong Agricultural University Wuhan 430070 China. National Engineering Research Center For Wheat Henan Agricultural University Zhengzhou 450002 China

Analyses of the mating-type ratio of protoplast monokaryons to spores in 2. strains of Lentinula edodes were performed through statistical methods. The results showed that the segregation distortion phenomenon commonly exists in both types of Lentinula edodes, 1. out of the 2. samples did not display the expected 1.1.ratio of protoplast monokaryons; and among them 8 fruiting strains distorted 1.1.1.1.segregation of spores. The degree of distortion in all tested wild strains was significantly lower than that of cultured strains. In fruiting test of positive-negative parent, predominant spores were those with the mating type identical to dikaryotic parent, indicating that the predominance of nuclei mainly depends on the mating type of dikaryotic parent. Data from di-mon mating test verified that monokaryons with acceptor nuclear type are present exclusively in a large amount after dedikaryotization, suggesting that the specificities among B factors and some other unknown factors in cytoplasm are probably responsible for the phenomenon of skewed segregation.

关键词： Lentinula edodes mating-type factors segregation distortion cytoplasm.

A current-mode RF transmitter for 6–9 GHz MB-OFDM UWB application

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Science china(Information Sciences) 2011年第2期54卷 419-428页

作者： CHEN YunFeng1, FAN JinHan1, li Wei1, li {2. & REN JunYan1,2.1state key {2. of ASIC & System, Fudan university, Shanghai 2.12.3, china 2.icro-/Nano-Electronics Science and Technology Innovation Platform, Fudan university, Shanghai 2.12.3, china 1. state {1. {2. of ASIC & System Fudan University Shanghai 201203 China2. Micro-/Nano-Electronics Science and Technology Innovation Platform Fudan University Shanghai 201203 China

This paper presents a current-mode 6–9 GHz RF transmitter for MB-OFDM UWB systems. The proposed current-mode transmitter consists of current-mode IQ LPF, current mirror amplifier, IQ up-conversion mixer (up-mixer), differential power driver amplifier (PA) and high-speed divider with LO buffers for generating IQ LO signals. All circuits are designed to possess high linearity and wide bandwidth. The transmitter is fabricated in TSMC 0.1. μm 1.8M RF CMOS technology with a die area of 1.56 mm2. Measurement results show that a maximum output power of -0.7 dBm with an LO leakage/sideband rejection ratio (LOL/SB RR) around -33/ - 35 dBc is achieved, and that this transmitter excels the traditional voltage-mode counterpart in terms of high linearity (about +1.dBm output 1.dB compression point, +1. dBm output IP3 in average) with comparable power consumption (drawing 48 mA current from 1.2.V supply).

关键词： current-mode RF transmitter high linearity MB-OFDM UWB high speed divider CMOS

A 6.2.9.5 GHz receiver for Wimedia MB-OFDM and{1. UWB standard

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Science china(Information Sciences) 2011年第2期54卷 407-418页

作者： GAO Ting1, ZHOU Feng1, li Wei1, LAN Fei1, li {2. & REN JunYan1,2.1state key {2. of ASIC & System, Fudan university, Shanghai 2.12.3, china 2.icro-/Nano-Electronics Science and Technology Innovation Platform, Shanghai 2.12.3, china 1. state {1. {2. of ASIC & System Fudan University Shanghai 201203 China2. Micro-/Nano-Electronics Science and Technology Innovation Platform Shanghai 201203 China

An integrated 6.2.9.5 GHz UWB receiver for both WiMedia MB-OFDM and{1. UWB standard is proposed. The receiver can cover Group#3, Group#4 for WiMedia and Group#2.5 for{1. UWB standard respectively. The fully differential receiver consists of a wideband LNA and a down conversion mixer with high gain and low gain mode. A low-pass filter with controllable gain and cut-o? frequency along with a programmable gain amp{2.fier are also included. The chip was fabricated in TSMC 0.1.-μm RF CMOS process. Measurement results show that the receiver achieves voltage gain from 2. to 69 dB with 2.dB/step and an NF of 3.2.4.6 dB over 6.2.9.5 GHz and 1.dB compression point of ?2. dBm. The intermediate frequency of the receiver can be switched between 2.4 and 1.2.MHz, which enables the receiver app{2.cable for both WiMedia and{1. UWB standard. Power consumption is only 47 mA with 1.2.V supply voltage.

关键词： CMOS ultra-wideband (UWB) MB-OFDM china UWB standard radio frequency (RF) low noise amplifier (LNA) quadrature mixer low-pass filter (LPF) Gm-C noise figure

Polystyrene nanoplastics induce glyco{1.pid metabo{1.sm disorder via NF-κB and MAPK signa{1.ng pathway in mice

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Journal of Environmental Sciences 2024年第3期137卷 553-566页

作者： Xingpei Fan Xiaoyan li Jiaxin li Yuxia Zhang Xiangjuan Wei Hailong Hu Boya Zhang Hai{2. du Meimei Zhao Ruijiao Zhu Daqian Yang Yuri Oh {2. Gu School of life Science and Technology Harbin Institute of TechnologyHarbin 150001China state {1. {2. of Urban Water Resource and Environment Harbin Institute of TechnologyHarbin 150006China Department of Medicine Renal Electrolyte and Hypertension DivisionDepartment of GeneticsPerelman School of MedicineUniversity of PennsylvaniaPhiladelphia 19104USA Faculty of Education Wakayama UniversityWakayama 640-8441Japan

Nanoplastics-induced developmental and reproductive toxicity,neurotoxicity and immunotoxicity are a focus of widespread ***,the effects of nanoplastics(NPs)on glycolipid metabolism and the precise underlying mechanisms are unclear at ***,we showed that oral administration of polystyrene nanoparticles(PS-NPs)disrupts glycolipid metabolism,with reactive oxygen species(ROS)identified as a potential key signaling *** PS-NPs treatment,excessive production of ROS induced the infammatory response and activated the antioxidant pathway through nuclear factor-erythroid factor 2.related factor *** activation of nuclear factor-κB(NFκB)signaling pathway induced the phosphorylation of the mitogen-activated protein kinases(MAPK)signaling pathway,which induced the activation of extracellular regulated kinases(ERK)and *** activation of the MAPK signaling proteins induced high continued phosphorylation of insulin receptor substrate-1,in turn,leading to decreased protein kinase B(Akt)activity,which weakened the sensitivity of liver cells to insulin signals and induced insulin *** parallel,phosphorylation of Akt led to loss of control of Fo XO1,a key gene of gluconeogenesis,activating transcription of glucose-6-phosphatase(G6PC)and phosphoenolpyruvate carboxykinase(PEPCK)in a manner dependent on PGC1α.Moreover,the activated ERK promoted lipid accumulation through ERK-PPARγ***,sterol regulatory element-binding protein-1 and levels of its downstream lipogenic enzymes,ACC-1,were *** treatment with the antioxidant resveratrol,PS-NPs-induced glucose and lipid metabolic disorders were improved by inhibiting ROS-induced activation of NFκB and MAPK signaling pathway in *** on above,PS-NPs exposure disrupts glycolipid metabolism in mice,with ROS identified as a potential key signaling molecule.

关键词： Nanoplastics Resveratrol Hyperglycemia lipid metabolism Infammatory response Reactive oxygen species(ROS)

A formal semantics for program debugging

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Science china(Information Sciences) 2012年第1期55卷 133-148页

作者： li Wei1.li {2.,2.1.4} key {2. of Software Development Environment,School of Computer Science and Engineering,Beihang university,Beijing 1.01.1.china 2.7} Ship Research and Development Academy,Beijing 1.01.2.china 1. state {1. {2. of Software Development Environment School of Computer Science and Engineering Beihang University Beijing 100191 China2. China Ship Research and Development Academy Beijing 100192 China

This work aims to build a semantic framework for automated debugging.A debugging process consists of tracing,locating,and fixing processes *** first two processes are accomplished by a tracing proce{1.re and a locating proce{1.re,*** tracing proce{1.re repro{1.ces the execution of the failed test case with well-designed data structures and saves necessary information for locating *** locating proce{1.re will use the information obtained from the tracing proce{1.re to locate ill-designed statements and to generate a system of fix-equations,whose solution will be used to fix the bugs.A structural operational semantics is given to define the functions of the tracing and locating *** of them are proved to *** main task of fixing process is to solve the *** turns out that for a given failed test case,there exist four types of fix-equations and three dierent solutions:1.The bug is solvable,i.e.,there exists a solution of the system of fix-equations,and the program can be repaired.2.There exists a structural design error in the program,i.e.,the system of fix-equations generated at each round of the locating proce{1.re is solvable,but a new bug will arise when the old bug is being fixed.3)There exists a logical design error,and the system of fix-equations is not solvable.

关键词： debugging operational semantics failed test case fix-equations

Numerical Simulation of Pollutant Transport and Accumulation Areas in the Hangzhou Bay

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Transactions of Tianjin university 2009年第6期15卷 400-407页

作者：李宁毛志华张庆河 School of Civil Engineering Tianjin University state {1. {2. of Satellite Ocean Environment Dynamics The Second Institute of Oceanography State Oceanic Administration

Based on the COHERENS model (a coupled hydrodynamic ecological model for regional and shelf seas), a numerical hydrodynamic model of the Hangzhou Bay, influenced by tide, regional winds and freshwater from the Yangtze River and the Qiantangjiang River was established. The Lagrangian particle tracking was simulated to provide tracer trajectories. For convenience, the modeling area was divided into 8 subdomains and the modeling focused on March (dry season) and July (wet season). Numerical simulation and analysis indicate that the tracer trajectories originated in different subdomains are quite different. Most particles released in the mouth of the bay move outside the bay quickly and reach the farthest place at 1.3}.5°E; while particles released in the inner part of the bay mostly remain in the same subdomain, with only minor migrations in two opposite directions along the shore. The tracer experiments also indicate that the northwest region of the bay is an area where pollutant can easily accumulate in both wet and dry seasons, and that the southeast region of the bay is another area for pollutant to accumulate in dry season because it is the main path for the contaminant.

关键词： Hangzhou Bay pollutant transport tracer trajectory COHERENS model wet and dry seasons

Layer-by-layer assembly carbon nanotubes thin film based gas sensors for ammonia detection

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Science china(Information Sciences) 2011年第12期54卷 2700-2706页

作者： SUN Ping 1,2., JIANG YaDong 1* , XIE GuangZhong 1 , du XiaoSong 1 , li Xian 1 & HU Jia 1 1 School of Optoelectronic Information, state {2. laboratory of Electronic Thin Films and Integrated Devices, {2. of Electronic Science and Technology of china (UESTC), Chengdu, 610054, china 2.life and Physical Science College, Sichuan agricultural {2., Ya’an 62.014, china 1. School of Optoelectronic Information State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of China (UESTC) Chengdu 610054 China2. Life and Physical Science College Sichuan Agricultural University Ya’an 625014 China

Single walled carbon nanotubes (SWNTs) were deposited on interdigital electrode (IDE) devices and quartz crystal microbalance (QCM) devices through layer-by-layer self-assembly method to fabricate resistance sensitive and mass sensitive gas sensors, respectively. Their ammonia gas-sensitive characteristics were detected at different temperature. It is revealed that the SWNTs based mass sensitive gas sensor has good sensing properties at room temperature. However, the SWNTs based resistance sensitive gas sensor has irreversible response for ammonia gas. When the temperature increases, the noise of SWNTs based mass sensitive gas sensor increases and the gas sensitive characteristic decreases. Meanwhile, the humidity effect is also discussed. It is important to optimize gas sensor by making it work at an appropriate external environment.

关键词： layer-by-layer self-assembly carbon nanotube gas sensor

A membrane-tethered transcription factor ANAC089 negatively regulates floral initiation in Arabidopsis thaliana

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Science china({1.fe Sciences) 2010年第11期53卷 1299-1306页

作者： li JianQin1,2. ZHANG Juan1, WANG XueChen1 & CHEN Jia1 1 state {2. laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,{2., Beijing 100193, china 2.College of life Science and Technology, Guangxi university, Nanning 530005, china 1. state {1. {2. of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100193 China2. College of Life Science and Technology Guangxi University Nanning 530005 China

The plant-specific NAC (NAM, ATAF1.2.and CUC2. transcription factors have a regulatory function in developmental processes and stress responses. Notably a group of NAC members named NTLs (NTM1.like) are membrane-tethered, ensuring plants rapidly respond to developmental changes and environmental stimuli. Our results indicated that ANAC089 was a membrane-tethered transcription factor and its truncated form was responsible for the physiological function in flowering time control.

关键词： Arabidopsis thaliana ANAC089 floral initiation membrane-tethered transcription factor